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The map label for this gene is pbp2A [H]

Identifier: 221232732

GI number: 221232732

Start: 1973324

End: 1975519

Strand: Reverse

Name: pbp2A [H]

Synonym: SPN23F_20310

Alternate gene names: 221232732

Gene position: 1975519-1973324 (Counterclockwise)

Preceding gene: 221232734

Following gene: 221232731

Centisome position: 88.93

GC content: 41.35

Gene sequence:

>2196_bases
ATGAAATTAGATAAATTATTTGAGAAATTTCTTTCTCTTTTTAAAAAAGAAACAAGTGAACTAGAGGACTCTGATTCTAC
TATCTTACGTCGCTCTCGTAGTGATCGAAAAAAATTAGCCCAAGTAGGTCCGATTCGAAAATTCTGGCGTCGTTATCATC
TAACAAAGATTATCCTTATACTAGGTTTGAGTGCAGGCTTGCTAGTTGGAATCTATTTGTTTGCTGTAGCCAAGTCGACC
AATGTCAATGATTTGCAAAATGCCTTGAAAACTCGGACTCTTATTTTTGACCGTGAAGAAAAAGAGGCTGGTGCCTTGTC
TGGTCAAAAGGGAACCTATGTTGAGCTGACTGACATCAGTAAAAACTTGCAGAATGCTGTTATTGCGACAGAAGACCGTT
CTTTCTATAAAAATGACGGGATTAACTATGGCCGTTTCTTCTTGGCTATTGTCACTGCTGGACGTTCAGGTGGTGGCTCT
ACCATTACCCAACAGCTGGCTAAAAACGCCTATTTATCGCAGGATCAAACTGTTGAGAGAAAAGCGAAAGAATTTTTCCT
TGCCTTAGAATTAAGCAAAAAATATAGTAAGGAGCAAATTCTAACCATGTACCTTAACAACGCTTATTTTGGAAATGGTG
TGTGGGGTGTAGAAGATGCGAGTAAGAAATACTTTGGAGTTTCTGCATCAGAAGTGAGTCTGGATCAAGCTGCGACTCTG
GCAGGGATGCTCAAGGGGCCGGAACTGTATAATCCCTTGAATTCCGTAGAAGATTCTACTAATCGGCGCGATACTGTCTT
GAAGAATATGGTTGCAGCAGGATATATTGATAAAAACCAAGAAACCGAAGCTGCTGAAGTTGATATGACTTCGCAATTGC
ACGATAAGTATGAAGGAAAAATCTCAGATTACCGTTACCCCTCTTATTTTGATGCGGTGGTTAATGAAGCTGTTTCCAAG
TATAATCTAACAGAGGAAGAGATTGTCAATAATGGCTACCGCATTTACACAGAGCTGGACCAAAACTACCAAGCAAATAT
GCAGATTGTTTATGAAAACACATCGCTATTTCCGAGGGCAGAGGATGGAACGTTTGCTCAATCAGGAAGTGTAGCTCTCG
AACCGAAAACAGGGGGAGTTCGTGGAGTTGTCGGTCAAGTTGCTGACAATGATAAAACTGGATTCCGGAATTTCAACTAT
GCAACCCAATCAAAGCGTAGTCCTGGTTCTACAATTAAGCCTTTAGTTGTTTATACGCCAGCAGTTGAAGCAGGCTGGGC
TTTGAATAAGCAGTTGGATAACCATACCATGCAGTATAACAGCTATAAGGTTGATAACTATGCAGGGATCAAAACGAGTC
GAGAAGTTCCTATGTATCAAGCCTTGGCAGAATCGCTTAATCTACCTGCTGTTGCCACTGTTAATGATTTGGGTGTTGAC
AAGGCTTTTGAGGCAGGCGAAAAATTCGGACTCAACATGGAAAAGGTCGACCGTGTTCTTGGTGTCGCCTTGGGAAGCGG
TGTTGAAACCAACCCTCTTCAAATGGCTCAAGCATACGCTGCCTTTGCAAATGAAGGTTTAATGCCTGAAGCTCATTTTA
TTAGTAGAATTGAAAATGCTAGTGGACAAGTTATTGCGAGTCATAAAAATTCACAAAAACGGGTGATTGATAAGTCTGTA
GCTGACAAGATGACCAGTATGATGTTGGGGACTTTCACCAACGGTACCGGTATTAGTTCATCGCCTGCAGACTATATTAT
GGCAGGGAAAACTGGAACAACTGAAGCAGTTTTCAATCCGGAGTACACAAGTGACCAGTGGGTAATTGGTTATACTCCGG
ATGTAGTGATTAGCCACTGGCTTGGCTTTCCTACCACTGATGAAAATCACTATCTAGCTGGCTCTACTTCAAACGGTGCA
GCTCATGTCTTTAGAAACATTGCCAATACCATTTTACCTTATACGCCAGGAAGTACCTTTACGGTTGAAAATGCTTATAA
GCAAAATGGAATTGCACCAGCCAATACAAAAAGACAAGTACAAACCAATGATAATAGCCAGACAGATGATAATTTGTCTG
ATATTCGAGGGCGTGCGCAAAGTCTAGTAGATGAGGCTAGCCGGGCTATCTCAGATGCGAAGATTAAGGAAAAGGCTCAA
ACAATATGGGATTCGATAGTCAATCTATTTCGTTAA

Upstream 100 bases:

>100_bases
AATGTGAATTGCATACAGATATTGTAACAAAAAAAGCCCTATTTGGATAGGAAATAGCTAAATTCTTGTCTTCCTATGAT
GAAGATGATAAAATAAACGC

Downstream 100 bases:

>100_bases
GATGCTTGTCAAAGCCTAGCTTTCTTGTTATAATGGATAAGATGGAGGCGTTATGGCACTAAAAAAAGCAAGCCTAGCTT
GTGCGGTTTGTGGTTCGAGA

Product: penicillin-binding protein 2a

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGPIRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKST
NVNDLQNALKTRTLIFDREEKEAGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVEDASKKYFGVSASEVSLDQAATL
AGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYIDKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSK
YNLTEEEIVNNGYRIYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDKTGFRNFNY
ATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYNSYKVDNYAGIKTSREVPMYQALAESLNLPAVATVNDLGVD
KAFEAGEKFGLNMEKVDRVLGVALGSGVETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSV
ADKMTSMMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFPTTDENHYLAGSTSNGA
AHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQTNDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQ
TIWDSIVNLFR

Sequences:

>Translated_731_residues
MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGPIRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKST
NVNDLQNALKTRTLIFDREEKEAGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVEDASKKYFGVSASEVSLDQAATL
AGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYIDKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSK
YNLTEEEIVNNGYRIYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDKTGFRNFNY
ATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYNSYKVDNYAGIKTSREVPMYQALAESLNLPAVATVNDLGVD
KAFEAGEKFGLNMEKVDRVLGVALGSGVETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSV
ADKMTSMMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFPTTDENHYLAGSTSNGA
AHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQTNDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQ
TIWDSIVNLFR
>Mature_731_residues
MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGPIRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKST
NVNDLQNALKTRTLIFDREEKEAGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVEDASKKYFGVSASEVSLDQAATL
AGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYIDKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSK
YNLTEEEIVNNGYRIYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDKTGFRNFNY
ATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYNSYKVDNYAGIKTSREVPMYQALAESLNLPAVATVNDLGVD
KAFEAGEKFGLNMEKVDRVLGVALGSGVETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSV
ADKMTSMMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFPTTDENHYLAGSTSNGA
AHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQTNDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQ
TIWDSIVNLFR

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=563, Percent_Identity=28.0639431616341, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI87082258, Length=311, Percent_Identity=30.8681672025724, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1788867, Length=535, Percent_Identity=27.6635514018692, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1789601, Length=166, Percent_Identity=35.5421686746988, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 80796; Mature: 80796

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGPIRKFWRRYHLTKIILI
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKEAGALSGQKGTYVELTDIS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCHHHHCCCCCCCCCEEEEHHHH
KNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGSTITQQLAKNAYLSQDQTVER
HHHHHCEEEECCCCHHHCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHH
KAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVEDASKKYFGVSASEVSLDQAATL
HHHHHHHHHHHHHHCCHHHEEHHEECCCEECCCCCCCCCCCCHHCCCCHHHHHHHHHHHH
AGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYIDKNQETEAAEVDMTSQLHDKYEGK
HHHHCCCHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
ISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYRIYTELDQNYQANMQIVYENTSLFPRA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCCC
EDGTFAQSGSVALEPKTGGVRGVVGQVADNDKTGFRNFNYATQSKRSPGSTIKPLVVYTP
CCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEECC
AVEAGWALNKQLDNHTMQYNSYKVDNYAGIKTSREVPMYQALAESLNLPAVATVNDLGVD
CCCCCCHHHHHHCCCEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHCCCH
KAFEAGEKFGLNMEKVDRVLGVALGSGVETNPLQMAQAYAAFANEGLMPEAHFISRIENA
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
SGQVIASHKNSQKRVIDKSVADKMTSMMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNP
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCEEECC
EYTSDQWVIGYTPDVVISHWLGFPTTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTF
CCCCCCEEEECCHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCE
TVENAYKQNGIAPANTKRQVQTNDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQ
EECCHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIWDSIVNLFR
HHHHHHHHHHC
>Mature Secondary Structure
MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGPIRKFWRRYHLTKIILI
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKEAGALSGQKGTYVELTDIS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCHHHHCCCCCCCCCEEEEHHHH
KNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGSTITQQLAKNAYLSQDQTVER
HHHHHCEEEECCCCHHHCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHH
KAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVEDASKKYFGVSASEVSLDQAATL
HHHHHHHHHHHHHHCCHHHEEHHEECCCEECCCCCCCCCCCCHHCCCCHHHHHHHHHHHH
AGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYIDKNQETEAAEVDMTSQLHDKYEGK
HHHHCCCHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
ISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYRIYTELDQNYQANMQIVYENTSLFPRA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCCC
EDGTFAQSGSVALEPKTGGVRGVVGQVADNDKTGFRNFNYATQSKRSPGSTIKPLVVYTP
CCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEECC
AVEAGWALNKQLDNHTMQYNSYKVDNYAGIKTSREVPMYQALAESLNLPAVATVNDLGVD
CCCCCCHHHHHHCCCEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHCCCH
KAFEAGEKFGLNMEKVDRVLGVALGSGVETNPLQMAQAYAAFANEGLMPEAHFISRIENA
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
SGQVIASHKNSQKRVIDKSVADKMTSMMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNP
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCEEECC
EYTSDQWVIGYTPDVVISHWLGFPTTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTF
CCCCCCEEEECCHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCE
TVENAYKQNGIAPANTKRQVQTNDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQ
EECCHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIWDSIVNLFR
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]