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The map label for this gene is nfsA [H]

Identifier: 221232446

GI number: 221232446

Start: 1653824

End: 1654537

Strand: Reverse

Name: nfsA [H]

Synonym: SPN23F_17110

Alternate gene names: 221232446

Gene position: 1654537-1653824 (Counterclockwise)

Preceding gene: 221232447

Following gene: 221232445

Centisome position: 74.48

GC content: 45.52

Gene sequence:

>714_bases
ATGACAGAAACCATTAAATTGATGAAGGCTCATACTTCAGTGCGCAGGTTTAAAGAGCAAGAAATTCCCCAAGTAGACTT
AAATGAGATTTTGACAGCAGCCCAGATGGCATCATCTTGGAAGAATTTCCAATCCTACTCTGTGATTGTGGTACGAAGTC
AAGAGAAGAAAGATGCCTTGTATGAATTGGTACCTCAAGAAGCCATTCGCCAGTCTGCTGTTTTCCTTCTCTTTGTCGGA
GATTTGAACCGAGCAGAAAAGGGAGCCAGACTTCATACCGACACCTTCCAACCCCAAGGTGTGGAAGGTCTCTTGATTAG
TTCGGTCGATGCAGCTCTTGCTGGACAAAACGCCTTGTTGGCAGCTGAAAGCTTGGGCTATGGTGGTGTGATTATCGGTT
TGGTTCGATACAAGTCTGAAGAAGTGGCAGAGCTCTTTAACCTACCTGACTACACCTATTCTGTCTTTGGGATGGCACTG
GGTGTGCCAAATCAACATCATGATATGAAACCGAGACTGCCACTAGAGAATGTTGTCTTTGAGGAAGAATACCAAGAACA
GTCAACTGAGGCAATCCAAGCTTATGACCGTGTTCAGGCTGACTATGCTGGGGCGCGTGCGACCACAAGCTGGAGTCAGC
GCCTAGCAGAACAGTTTGGTCAAGCTGAACCAAGCTCAACTAGAAAAAATCTTGAACAGAAGAAATTATTGTAG

Upstream 100 bases:

>100_bases
AAGTGAAACATGGAAATGACGAAACCTGGGAAGCCAGACGGGTCATGGAACGCATCCGTTATTTGGCTGAGGAGACTCGT
TTAGAAGGAGTAAACCGTCG

Downstream 100 bases:

>100_bases
AAAGTGAGAAATTATGGCCCTACCAACTATTGCCATTGTAGGACGTCCCAATGTTGGGAAATCAACCCTATTTAATCGGA
TCGCTGGTGAGCGAATCTCC

Product: oxygen-insensitive NADPH nitroreductase

Products: NA

Alternate protein names: NAD(P)H-dependent FMN reductase; NAD(P)H-dependent nitroreductase; NAD(P)H-dependent oxidoreductase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVG
DLNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL

Sequences:

>Translated_237_residues
MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVG
DLNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL
>Mature_236_residues
TETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGD
LNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMALG
VPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones, chromates and azo dyes. It could supply the reduced form of FMN to luciferase-like protein and contribute to the degradation of aromatic compounds [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=220, Percent_Identity=35.9090909090909, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 26544; Mature: 26413

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDAL
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHH
YELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALL
HHHCCHHHHHHHHEEEEEECCCCCHHCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCHHE
AAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMALGVPNQHHDMKPRLPLENVVF
EHHHCCCCHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
EEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDAL
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHH
YELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALL
HHHCCHHHHHHHHEEEEEECCCCCHHCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCHHE
AAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMALGVPNQHHDMKPRLPLENVVF
EHHHCCCCHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
EEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]