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The map label for this gene is nanH [H]

Identifier: 221232413

GI number: 221232413

Start: 1616449

End: 1617366

Strand: Reverse

Name: nanH [H]

Synonym: SPN23F_16770

Alternate gene names: 221232413

Gene position: 1617366-1616449 (Counterclockwise)

Preceding gene: 221232414

Following gene: 221232412

Centisome position: 72.81

GC content: 43.03

Gene sequence:

>918_bases
ATGTCAGATTTGAAAAAATACGAAGGTGTCATTCCAGCCTTCTACGCATGTTATGATGAGCAAGGAGAAGTAAGTCCAGA
GCGTACGCGTGCCTTGGTTCAATACTTCATTGATAAAGGTGTTCAAGGTCTTTATGTCAATGGTTCTTCTGGTGAATGTA
TCTACCAAAGCGTTGAAGATCGCAAGTTGATTTTGGAAGAAGTCATGGCGGTAGCCAAAGGTAAATTGACCATTATTGCC
CATGTTGCTTGCAATAATACTAAAGATAGTATGGAACTTGCTCGCCATGCTGAAAGCTTGGGAGTAGATGCTATTGCAAC
GATTCCACCAATTTACTTCCGCTTGCCAGAATACTCAGTTGCCAAATACTGGAACGATATTAGTTCTGCAGCTCCAAACA
CAGACTACGTGATTTACAACATTCCTCAATTGGCAGGGGTTGCTTTGACTTCAAGCCTTTACACAGAAATGTTGAAAAAT
CCTCGTGTTATCGGTGTTAAGAACTCTTCAATGCCAGTTCAAGATATCCAAACCTTTGTCAGCCTTGGCGGGGAAGACCA
TATTGTCTTTAACGGTCCAGATGAACAGTTCCTAGGTGGCCGCCTCATGGGTGCTAAGGCTGGTATCGGTGGTACTTATG
GTGCTATGCCAGAACTCTTCTTGAAACTCAATCAGTTGATTGCGGATAAAGATTTAGAAACAGCGCGTGAATTGCAGTAT
GCTATCAACGCAATCATTGGTAAACTCACTGCTGCACATGGAAATATGTACGGTGTCATCAAAGAAGTTTTGAAGATCAA
TGAAGGCTTGAATATTGGTTCTGTTCGTTCACCATTGACACCAGTGACTGAAGACGATCGTCCAGTTGTAGAAGCGGCTG
CAGCCTTGATTCGTGAAACCAAGGAGCGCTTCCTCTAA

Upstream 100 bases:

>100_bases
AGTGTCTATAAGATATTGAAAGCCTTTTCGGTAAAGTGTATACTAAAGAAGTAAAGAAAAATCTGCTTTAGCAGAAAATA
AAAATAATAGGAGAAAATCT

Downstream 100 bases:

>100_bases
TCTAAAAGGAGGTATTTATGACATACTACGTTGCAATTGATATCGGTGGAACTAACATCAAGTATGGTTTGGTTGATCAA
GAGGGGCAACTTCTTGAATC

Product: N-acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGLYVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIA
HVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKN
PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQY
AINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVRSPLTPVTEDDRPVVEAAAALIRETKERFL

Sequences:

>Translated_305_residues
MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGLYVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIA
HVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKN
PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQY
AINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVRSPLTPVTEDDRPVVEAAAALIRETKERFL
>Mature_304_residues
SDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGLYVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAH
VACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKNP
RVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQYA
INAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVRSPLTPVTEDDRPVVEAAAALIRETKERFL

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=300, Percent_Identity=29.3333333333333, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1789620, Length=293, Percent_Identity=32.4232081911263, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI87082415, Length=240, Percent_Identity=26.6666666666667, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786463, Length=234, Percent_Identity=25.6410256410256, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788823, Length=289, Percent_Identity=21.7993079584775, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 33372; Mature: 33240

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGLYVNGSSGECIYQSVED
CCCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
RKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSV
HHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCEEECCCHHH
AKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKNPRVIGVKNSSMPVQDIQTFV
HHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
SLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQY
HCCCCCEEEECCCCHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVRSPLTPVTEDDRPVVEAAAALIRET
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KERFL
HHHCC
>Mature Secondary Structure 
SDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGLYVNGSSGECIYQSVED
CCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
RKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSV
HHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCEEECCCHHH
AKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKNPRVIGVKNSSMPVQDIQTFV
HHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
SLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQY
HCCCCCEEEECCCCHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVRSPLTPVTEDDRPVVEAAAALIRET
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KERFL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA