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The map label for this gene is trmB

Identifier: 221230894

GI number: 221230894

Start: 3132445

End: 3133137

Strand: Reverse

Name: trmB

Synonym: MLBr_02622

Alternate gene names: 221230894

Gene position: 3133137-3132445 (Counterclockwise)

Preceding gene: 221230897

Following gene: 221230893

Centisome position: 95.87

GC content: 59.74

Gene sequence:

>693_bases
GTGGCACGCCATCTACCAGCCACTGCTTTTCGTAAGCGACGCTCCGCCTTATCACACGCCCAGCGGCAGACCTGGGAGCG
GTTGTGGCCGGAAATAGGCATTTCGGCGGTGTCCCAAACCCGCAGCGCCGAGCGGCTAGACACCGGAGCGTGGTTCGGGC
GTTCCACCTTAGTGGTACTCGAGGTCGGCTGCGGCAGCGGCACTGCAACGCTGGCCATGGCGCAGCACGAACCCGACATC
GATGTGATCGCAGTAGAAGTTTACCGTCGCGGATTGGCGCAGCTGTTGTGCGCGATCGACCGAGACCAGGTGCACAACAT
CCGTATGATTCATGGGAACGCGCTCTACGTGCTGCAGTACCTTATCGCACCAAGGTCGCTGACCGGTGTTCGTGTCTTTT
TTCCCGATCCATGGCCGAAGGTTCGCCACCACAAACGCAGGTTCCTACAACCGGCCACGGTTGAACTGATCGCTGACCGG
CTACTCCCCGGTGGGGTCCTGCACACCGCGACGGACCACCCCGACTACGCAAAACAAATCGCCAAATTCGGCGACGGCGA
GCCACTGCTGAGTCGTGCTGATGGCAGAACCCAGCTGCCAATTTCAACCGTTCGCCCAACCACCAAGTACGAAACGAAAG
CGCAGCATGCCGGCAACGTCGTTACCGAGTTGATTTGGAAAAAACGCAGTTGA

Upstream 100 bases:

>100_bases
GGCGCGGAACCCAGCTAGCCCAAGCTGCGGAGCGGACTCGCACCCGTCGTTAGAGCCGCGTGAGACAAACCACTCTGAGC
CTGGCTTCGAACAAAGTGGT

Downstream 100 bases:

>100_bases
TTTGCAAAAAGTGAAGTTGATTTGCCGAAAACTCGAAATTGATCTCGAAAGCAATGCAATCGATCTTGATAAACCGATGA
CTATCCCAGTGACCGAAGAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MARHLPATAFRKRRSALSHAQRQTWERLWPEIGISAVSQTRSAERLDTGAWFGRSTLVVLEVGCGSGTATLAMAQHEPDI
DVIAVEVYRRGLAQLLCAIDRDQVHNIRMIHGNALYVLQYLIAPRSLTGVRVFFPDPWPKVRHHKRRFLQPATVELIADR
LLPGGVLHTATDHPDYAKQIAKFGDGEPLLSRADGRTQLPISTVRPTTKYETKAQHAGNVVTELIWKKRS

Sequences:

>Translated_230_residues
MARHLPATAFRKRRSALSHAQRQTWERLWPEIGISAVSQTRSAERLDTGAWFGRSTLVVLEVGCGSGTATLAMAQHEPDI
DVIAVEVYRRGLAQLLCAIDRDQVHNIRMIHGNALYVLQYLIAPRSLTGVRVFFPDPWPKVRHHKRRFLQPATVELIADR
LLPGGVLHTATDHPDYAKQIAKFGDGEPLLSRADGRTQLPISTVRPTTKYETKAQHAGNVVTELIWKKRS
>Mature_229_residues
ARHLPATAFRKRRSALSHAQRQTWERLWPEIGISAVSQTRSAERLDTGAWFGRSTLVVLEVGCGSGTATLAMAQHEPDID
VIAVEVYRRGLAQLLCAIDRDQVHNIRMIHGNALYVLQYLIAPRSLTGVRVFFPDPWPKVRHHKRRFLQPATVELIADRL
LPGGVLHTATDHPDYAKQIAKFGDGEPLLSRADGRTQLPISTVRPTTKYETKAQHAGNVVTELIWKKRS

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=223, Percent_Identity=34.9775784753363, Blast_Score=137, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_MYCLB (B8ZTI4)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002504310.1
- ProteinModelPortal:   B8ZTI4
- SMR:   B8ZTI4
- EnsemblBacteria:   EBMYCT00000087056
- GeneID:   7325534
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017066
- HOGENOM:   HBG322782
- OMA:   NIRLIRG
- ProtClustDB:   PRK00121
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25821; Mature: 25690

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: BINDING 61-61 BINDING 86-86 BINDING 113-113 BINDING 136-136 BINDING 140-140 BINDING 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARHLPATAFRKRRSALSHAQRQTWERLWPEIGISAVSQTRSAERLDTGAWFGRSTLVVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
EVGCGSGTATLAMAQHEPDIDVIAVEVYRRGLAQLLCAIDRDQVHNIRMIHGNALYVLQY
EECCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHCHHHCCEEEEEECCHHHHHHH
LIAPRSLTGVRVFFPDPWPKVRHHKRRFLQPATVELIADRLLPGGVLHTATDHPDYAKQI
HHCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHH
AKFGDGEPLLSRADGRTQLPISTVRPTTKYETKAQHAGNVVTELIWKKRS
HHCCCCCHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARHLPATAFRKRRSALSHAQRQTWERLWPEIGISAVSQTRSAERLDTGAWFGRSTLVVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
EVGCGSGTATLAMAQHEPDIDVIAVEVYRRGLAQLLCAIDRDQVHNIRMIHGNALYVLQY
EECCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHCHHHCCEEEEEECCHHHHHHH
LIAPRSLTGVRVFFPDPWPKVRHHKRRFLQPATVELIADRLLPGGVLHTATDHPDYAKQI
HHCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHH
AKFGDGEPLLSRADGRTQLPISTVRPTTKYETKAQHAGNVVTELIWKKRS
HHCCCCCHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA