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The map label for this gene is clpB

Identifier: 221230822

GI number: 221230822

Start: 2963087

End: 2965633

Strand: Reverse

Name: clpB

Synonym: MLBr_02490

Alternate gene names: 221230822

Gene position: 2965633-2963087 (Counterclockwise)

Preceding gene: 221230824

Following gene: 221230821

Centisome position: 90.75

GC content: 61.8

Gene sequence:

>2547_bases
GTGGACTCTTTCAACCCGACCACCAAAATGCAGGTGGCTCTAACTTCAGCGCTGCAGGCAGCGTCGTCCGCAGGTAATCC
CGAGATCAGACCTGTGCATTTGCTATTGGCGATGCTCATGCAAAACGACGGAATCGCCGCACCGTTGTTGGAGACTGTCG
GTGTGGAGCCCGACATCGTTCGCAATGAGGCACAGCGGCTCCTGGAGCGATTACCCCAGGCCAGCGGCTGCAGCTCGCAG
CCGCAGTTGTCGCGAGAGTCGTTGGCTGCCATCACCACCGCTCAGCAGCTCGCTACCGAGATGGACGACGAGTACGTGTC
GACCGAGCACCTGATGCTAGGGTTAGCCATGGGCGACTCCGATGTCGCGAAGCTGCTTACCGGGCACGGTGCCTCGCCGC
AGGTGTTACGGGAGGCGTTCGTCAAGATACGTGGCAGTGCCCGAGTCACTAGCTCCGATCCCGAGTTCACCTATCAAGCG
TTGGAGAAGTACTCCACAGATCTGACCGCCCAGTCCGGCGAAGGCAAACTTGACCCAGTTATCGGGCGCGACAACGAGAT
CCGTCGTGTTGTGCAGGTGCTGTCCCGTCGCACCAAGAACAACCCGGTGCTTATCGGCGAGCCTGGTGTCGGCAAGACCG
CGATCGTGGAGGGCTTGGCTCAGCGCATCGTTGCCGGCGATGTGCCGGAGAGCCTGCGTGACAAGACCTTGGTAGCGCTG
GATCTCGGGTCAATGGTGGCTGGCGCCAAGTATCGCGGCGAGTTCGAGCAACGACTCAAGGTCGTGCTCGACGACATCAA
ATTCTCTGCTGGACAGATCATCACGTTTATCGATGAGTTGCACACTATCGTCGGAGCCGGTGCGACTGGTGAGTCGGCGA
TGGATGCTGGCAACATGATCAAGCCGATGCTGGCCCGTGGCGAATTGCGCCTGGTCGGCGCCACCACGCTTGACGAGTAC
CGCAGGTACATCGAGAAGGATGCAGCGCTGGAGCGGCGCTTCCAGCAGATCTTCGTCGGCGAGCCGTCGGTGGAGGACAC
TGTTGGCATCCTGCGTGGGCTGAAGGACCGTTACGAGGTGCACCACGGTGTGCGCATCACCGACTCTGCTCTGGTAGCTG
CGGCAGCCCTCTCCGACCGTTATATCACCGCCCGGTTCCTGCCGGACAAGGCCATCGACCTGGTCGACGAAGCGGCTAGC
CGGTTACGCATGGAGATCGACTCGCGGCCTGTCGAGATCGACGAGGTTGAGCGGCTGGTGCGTCGCTTCGAGATCGAAGA
GATGGCTCTTGTGAACGAAGAAGACGAAGCATCCAAGGAGCGTTTGGAGACGTTGCGTGCGGAACTGGCTGACCAGAAGG
AGCGGCTCGCCGAGCTAACCGCGAGGTGGCAGAATGAAAAGAACGCGATAGATGTCGTCCGAGAACTTAAGGAACAGTTG
GAGACGTTGCGCGGGGAATCCGAGCGTGCTGAGCGTGACGGTGACCTGGCCAAGGCCGCTGAGCTGCGGTATGGGCGCAT
CCCCGAGGTTGAAAAGAAGCTGGAAGCGGCGGTGCCGACGGCCCAGGCCCGCGAGGACGTGATGCTCAAGGAAGAGGTCG
GTCCTGACGATATCGCCAACGTGGTGTCAGCGTGGACCGGCATACCGGCTGGCCGGTTGTTGGAGGGCGAGACCGCCAAG
CTGCTGCGTATGGAGGACGAACTGGGCAAGCGTGTGGTCGGGCAGAAGAAGGCAGTGCACGCGGTCTCTGACGCGGTGCG
ACGCTCGCGGGCCGGGGTTGCCGACCCTAACCGGCCGACCGGCTCGTTTTTGTTTCTTGGCCCGACCGGTGTCGGCAAGA
CCGAGCTGGCCAAAGCACTGGCTGATTTCCTGTTTGACGACCAGCGCGCGATGGTCCGTGTGGACATGAGCGAGTACGGT
GAGAAGCATTCGGTGGCCCGTCTGGTGGGTGCACCGCCCGGCTACATTGGCCACGATCAGGGTGGTCAGCTGACCGAGGC
GGTGCGCCGACGGCCTTACACGGTGGTGCTTTTCGACGAGGTCGAAAAGGCCCACTCGGATGTCTTCGATGTGCTACTGC
AGGTGCTCGACGAAGGCCGTTTGACTGACGGGCAGGGCCGCACGGTCGACTTTCGCAACACGATCCCCATCTTGACCTCC
AACCTGGGGTCGGGCGGCAGCGAGGAGCAGGTAATGGCAGCGGTGCGCTCGGTGTTCAAGCCGGAGTTCATCAACCGCCT
CGACGACGTACTGATCTTCGACGGGCTCAACCCCGAAGAGTTGGTGCGGATTGTCGACATTCAGCTTGAGCAGCTTGGCA
AGCGGCTCGCGCAGCGGCGGCTTCAACTGGAGGTTTCGTTGCCGGCTAAGCGCTGGTTGGCACAGCACGGCTTCGATCCT
GTATACGGCGCCCGGCCATTGCGTCGACTGGTACAGCAAGCGATCGGCGATCAATTGGCTAAAATGCTGCTGGCCGGCGA
GGTGCATGACGGGGACACCCTTCCGGTCAACGTCAGACCCGACGGGGAGGCGTTGATCCTGGGCTGA

Upstream 100 bases:

>100_bases
GGTCACCACCAAAGATAAAATTGAGTGGAACAGACTTAATGTTGACGGCGTTAAACAACCCTACAAGCATTTTTTAGACG
TACCCTGAATGGAGATCGTC

Downstream 100 bases:

>100_bases
TCGGCTCTAGGGAGTCGGCCTGTGACGGGGTTGTGATCTGATCGCATTCTCGGTTTCGGGCTGACAGCAGATACTCTGTC
TGGGATGGTTCCACTTTGGT

Product: heat shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 848; Mature: 848

Protein sequence:

>848_residues
MDSFNPTTKMQVALTSALQAASSAGNPEIRPVHLLLAMLMQNDGIAAPLLETVGVEPDIVRNEAQRLLERLPQASGCSSQ
PQLSRESLAAITTAQQLATEMDDEYVSTEHLMLGLAMGDSDVAKLLTGHGASPQVLREAFVKIRGSARVTSSDPEFTYQA
LEKYSTDLTAQSGEGKLDPVIGRDNEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRDKTLVAL
DLGSMVAGAKYRGEFEQRLKVVLDDIKFSAGQIITFIDELHTIVGAGATGESAMDAGNMIKPMLARGELRLVGATTLDEY
RRYIEKDAALERRFQQIFVGEPSVEDTVGILRGLKDRYEVHHGVRITDSALVAAAALSDRYITARFLPDKAIDLVDEAAS
RLRMEIDSRPVEIDEVERLVRRFEIEEMALVNEEDEASKERLETLRAELADQKERLAELTARWQNEKNAIDVVRELKEQL
ETLRGESERAERDGDLAKAAELRYGRIPEVEKKLEAAVPTAQAREDVMLKEEVGPDDIANVVSAWTGIPAGRLLEGETAK
LLRMEDELGKRVVGQKKAVHAVSDAVRRSRAGVADPNRPTGSFLFLGPTGVGKTELAKALADFLFDDQRAMVRVDMSEYG
EKHSVARLVGAPPGYIGHDQGGQLTEAVRRRPYTVVLFDEVEKAHSDVFDVLLQVLDEGRLTDGQGRTVDFRNTIPILTS
NLGSGGSEEQVMAAVRSVFKPEFINRLDDVLIFDGLNPEELVRIVDIQLEQLGKRLAQRRLQLEVSLPAKRWLAQHGFDP
VYGARPLRRLVQQAIGDQLAKMLLAGEVHDGDTLPVNVRPDGEALILG

Sequences:

>Translated_848_residues
MDSFNPTTKMQVALTSALQAASSAGNPEIRPVHLLLAMLMQNDGIAAPLLETVGVEPDIVRNEAQRLLERLPQASGCSSQ
PQLSRESLAAITTAQQLATEMDDEYVSTEHLMLGLAMGDSDVAKLLTGHGASPQVLREAFVKIRGSARVTSSDPEFTYQA
LEKYSTDLTAQSGEGKLDPVIGRDNEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRDKTLVAL
DLGSMVAGAKYRGEFEQRLKVVLDDIKFSAGQIITFIDELHTIVGAGATGESAMDAGNMIKPMLARGELRLVGATTLDEY
RRYIEKDAALERRFQQIFVGEPSVEDTVGILRGLKDRYEVHHGVRITDSALVAAAALSDRYITARFLPDKAIDLVDEAAS
RLRMEIDSRPVEIDEVERLVRRFEIEEMALVNEEDEASKERLETLRAELADQKERLAELTARWQNEKNAIDVVRELKEQL
ETLRGESERAERDGDLAKAAELRYGRIPEVEKKLEAAVPTAQAREDVMLKEEVGPDDIANVVSAWTGIPAGRLLEGETAK
LLRMEDELGKRVVGQKKAVHAVSDAVRRSRAGVADPNRPTGSFLFLGPTGVGKTELAKALADFLFDDQRAMVRVDMSEYG
EKHSVARLVGAPPGYIGHDQGGQLTEAVRRRPYTVVLFDEVEKAHSDVFDVLLQVLDEGRLTDGQGRTVDFRNTIPILTS
NLGSGGSEEQVMAAVRSVFKPEFINRLDDVLIFDGLNPEELVRIVDIQLEQLGKRLAQRRLQLEVSLPAKRWLAQHGFDP
VYGARPLRRLVQQAIGDQLAKMLLAGEVHDGDTLPVNVRPDGEALILG
>Mature_848_residues
MDSFNPTTKMQVALTSALQAASSAGNPEIRPVHLLLAMLMQNDGIAAPLLETVGVEPDIVRNEAQRLLERLPQASGCSSQ
PQLSRESLAAITTAQQLATEMDDEYVSTEHLMLGLAMGDSDVAKLLTGHGASPQVLREAFVKIRGSARVTSSDPEFTYQA
LEKYSTDLTAQSGEGKLDPVIGRDNEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRDKTLVAL
DLGSMVAGAKYRGEFEQRLKVVLDDIKFSAGQIITFIDELHTIVGAGATGESAMDAGNMIKPMLARGELRLVGATTLDEY
RRYIEKDAALERRFQQIFVGEPSVEDTVGILRGLKDRYEVHHGVRITDSALVAAAALSDRYITARFLPDKAIDLVDEAAS
RLRMEIDSRPVEIDEVERLVRRFEIEEMALVNEEDEASKERLETLRAELADQKERLAELTARWQNEKNAIDVVRELKEQL
ETLRGESERAERDGDLAKAAELRYGRIPEVEKKLEAAVPTAQAREDVMLKEEVGPDDIANVVSAWTGIPAGRLLEGETAK
LLRMEDELGKRVVGQKKAVHAVSDAVRRSRAGVADPNRPTGSFLFLGPTGVGKTELAKALADFLFDDQRAMVRVDMSEYG
EKHSVARLVGAPPGYIGHDQGGQLTEAVRRRPYTVVLFDEVEKAHSDVFDVLLQVLDEGRLTDGQGRTVDFRNTIPILTS
NLGSGGSEEQVMAAVRSVFKPEFINRLDDVLIFDGLNPEELVRIVDIQLEQLGKRLAQRRLQLEVSLPAKRWLAQHGFDP
VYGARPLRRLVQQAIGDQLAKMLLAGEVHDGDTLPVNVRPDGEALILG

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family

Homologues:

Organism=Homo sapiens, GI13540606, Length=327, Percent_Identity=33.9449541284404, Blast_Score=193, Evalue=6e-49,
Organism=Escherichia coli, GI1788943, Length=847, Percent_Identity=54.7815820543093, Blast_Score=914, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=408, Percent_Identity=41.1764705882353, Blast_Score=277, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=696, Percent_Identity=53.8793103448276, Blast_Score=752, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=862, Percent_Identity=42.3433874709977, Blast_Score=664, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPB_MYCLE (Q9CB26)

Other databases:

- EMBL:   AL583925
- PIR:   G87220
- RefSeq:   NP_302608.1
- ProteinModelPortal:   Q9CB26
- EnsemblBacteria:   EBMYCT00000028809
- GeneID:   908472
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2490
- NMPDR:   fig|272631.1.peg.1480
- Leproma:   ML2490
- GeneTree:   EBGT00070000031953
- HOGENOM:   HBG413133
- OMA:   GDQLAKK
- ProtClustDB:   CLSK790482
- BioCyc:   MLEP272631:ML2490-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- Gene3D:   G3DSA:1.10.1780.10
- PRINTS:   PR00300
- SMART:   SM00382
- TIGRFAMs:   TIGR03346

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 93208; Mature: 93208

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSFNPTTKMQVALTSALQAASSAGNPEIRPVHLLLAMLMQNDGIAAPLLETVGVEPDIV
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
RNEAQRLLERLPQASGCSSQPQLSRESLAAITTAQQLATEMDDEYVSTEHLMLGLAMGDS
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEECCCH
DVAKLLTGHGASPQVLREAFVKIRGSARVTSSDPEFTYQALEKYSTDLTAQSGEGKLDPV
HHHHHHHCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
IGRDNEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRDKTLVAL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHCCEEEEE
DLGSMVAGAKYRGEFEQRLKVVLDDIKFSAGQIITFIDELHTIVGAGATGESAMDAGNMI
EHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCHHH
KPMLARGELRLVGATTLDEYRRYIEKDAALERRFQQIFVGEPSVEDTVGILRGLKDRYEV
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HHGVRITDSALVAAAALSDRYITARFLPDKAIDLVDEAASRLRMEIDSRPVEIDEVERLV
HCCCEECHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RRFEIEEMALVNEEDEASKERLETLRAELADQKERLAELTARWQNEKNAIDVVRELKEQL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ETLRGESERAERDGDLAKAAELRYGRIPEVEKKLEAAVPTAQAREDVMLKEEVGPDDIAN
HHHCCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHH
VVSAWTGIPAGRLLEGETAKLLRMEDELGKRVVGQKKAVHAVSDAVRRSRAGVADPNRPT
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GSFLFLGPTGVGKTELAKALADFLFDDQRAMVRVDMSEYGEKHSVARLVGAPPGYIGHDQ
CCEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEEEHHHCCCHHHHHHHHCCCCCCCCCCC
GGQLTEAVRRRPYTVVLFDEVEKAHSDVFDVLLQVLDEGRLTDGQGRTVDFRNTIPILTS
CCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHCC
NLGSGGSEEQVMAAVRSVFKPEFINRLDDVLIFDGLNPEELVRIVDIQLEQLGKRLAQRR
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
LQLEVSLPAKRWLAQHGFDPVYGARPLRRLVQQAIGDQLAKMLLAGEVHDGDTLPVNVRP
HHEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
DGEALILG
CCCEEEEC
>Mature Secondary Structure
MDSFNPTTKMQVALTSALQAASSAGNPEIRPVHLLLAMLMQNDGIAAPLLETVGVEPDIV
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
RNEAQRLLERLPQASGCSSQPQLSRESLAAITTAQQLATEMDDEYVSTEHLMLGLAMGDS
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEECCCH
DVAKLLTGHGASPQVLREAFVKIRGSARVTSSDPEFTYQALEKYSTDLTAQSGEGKLDPV
HHHHHHHCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
IGRDNEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRDKTLVAL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHCCEEEEE
DLGSMVAGAKYRGEFEQRLKVVLDDIKFSAGQIITFIDELHTIVGAGATGESAMDAGNMI
EHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCHHH
KPMLARGELRLVGATTLDEYRRYIEKDAALERRFQQIFVGEPSVEDTVGILRGLKDRYEV
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HHGVRITDSALVAAAALSDRYITARFLPDKAIDLVDEAASRLRMEIDSRPVEIDEVERLV
HCCCEECHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RRFEIEEMALVNEEDEASKERLETLRAELADQKERLAELTARWQNEKNAIDVVRELKEQL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ETLRGESERAERDGDLAKAAELRYGRIPEVEKKLEAAVPTAQAREDVMLKEEVGPDDIAN
HHHCCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHH
VVSAWTGIPAGRLLEGETAKLLRMEDELGKRVVGQKKAVHAVSDAVRRSRAGVADPNRPT
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GSFLFLGPTGVGKTELAKALADFLFDDQRAMVRVDMSEYGEKHSVARLVGAPPGYIGHDQ
CCEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEEEHHHCCCHHHHHHHHCCCCCCCCCCC
GGQLTEAVRRRPYTVVLFDEVEKAHSDVFDVLLQVLDEGRLTDGQGRTVDFRNTIPILTS
CCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHCC
NLGSGGSEEQVMAAVRSVFKPEFINRLDDVLIFDGLNPEELVRIVDIQLEQLGKRLAQRR
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
LQLEVSLPAKRWLAQHGFDPVYGARPLRRLVQQAIGDQLAKMLLAGEVHDGDTLPVNVRP
HHEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
DGEALILG
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002