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The map label for this gene is mshA

Identifier: 221230798

GI number: 221230798

Start: 2917898

End: 2919184

Strand: Reverse

Name: mshA

Synonym: MLBr_02443

Alternate gene names: 221230798

Gene position: 2919184-2917898 (Counterclockwise)

Preceding gene: 221230799

Following gene: 221230797

Centisome position: 89.32

GC content: 64.18

Gene sequence:

>1287_bases
GTGCTGGCGGTGCACACCTCACCGCTGGCTCAGCCGGGCATCGGCGACGCAGGCGGCATGAATGTTTACGTGCTGCAGAG
CGCGTTGCACCTGGCTCGGCGTGGCATTGAGGTGGAGATCTTTACCCGGGCCACTGCCTCTGCCGATCCGCCCATCGTGT
GGGTAGCGCCCGGAGTGTTAGTGCGCAACGTCGTAGCAGGGCCGTTCGAGGGCCTGGATAAATACGACTTACCTACCCAG
CTGTGTGCGTTTGCTGCCGGGGTGCTGCGCGCAGAAGCGGCTCACGAACCGGGCTACTACGATATCGTCCACTCGCATTA
TTGGCTGTCTGGGCAGGTCGGCTGGCTGGCCCGGGACCGCTGGGCGGTGCCGTTGGTGCATACCGCGCACACGTTGGCCG
CGGTGAAGAACGCGGCGCTGGCCGATGGTGACGCGGCTGAGCCGCCGTTGCGTAGTGTCGGCGAACAGCAGGTCGTCGAC
GAGGCCGATCGGATGATCGTCAACACCGATGATGAAGCAAGGCAATTGATTTCGATTCACCGTGCCGATCCAGCGAAAAT
TGACGTGGCCCATCCTGGTGTTGATCTGGACATGTTCCGTCCAGGTGACCGGCGGGCTGCTCGGGCTGCGCTGGGGCTGC
CGCTCGACGGCAACGTGGTGGCCTTCGTCGGACGGATCCAGCCGTTGAAGGCACCTGACATCGTGTTACGTGCCGCCGCG
AAACTGCCGCAGGTGCGCATCGTTGTGGCTGGCGGACCGTCAGGCAGTGGTTTGGCCTCCCCGGACGGGCTGGTTAGGTT
GGCCGACGAACTTGGTATCACGGCCCGGGTGACGTTCTTGCCGCCGCAATCCCGTACGAATTTGGCTACAGTCTTCCAAG
CCGCCGATCTAGTTGCGGTGCCGAGTTATTCAGAGTCGTTTGGCCTGGTCGCCGTCGAGGCGCAGGCTTGTGGCACTCCG
GTGGTAGCGGCGGCGGTCGGCGGCTTGCCGGTAGCGGTGCGGGACGGGGTCACCGGCACTTTGGTGTTCGGACATAATGT
CGGTCATTGGGCGGACGCCGTCGATCAGCTGTTACGACTCAGTGCCGGGCCGCAGGCAAGGGCGATAAGTCGCGCGGCTG
TCGTGCATGCGGCCCAATTCTCCTGGGACAACACCACTGATGCGCTGCTAGCCAGCTACCGTCGTGCGATCGGTGATTTC
ACCGCGACGCGTCAGCATCGGGTCCGCGACTTGGTGGCAACGCGCAAGCCCCGTCGTTGGATCTCACGCCGTGGGATGGG
CGCATGA

Upstream 100 bases:

>100_bases
CCCGTTTCGGGCGGTTCCAGATCGGTCCTGTAAAGATGACTAATGTGCGGGCCATCGAATTGAAACGTTTCCGCCAGTGA
ATGATCTCCATCGGGTCGCG

Downstream 100 bases:

>100_bases
CCCGTACCGGCGACGATGCAGAACGAAGCCATGAGGCAGGGGCATCTCCTGCTTGCAGGGGAGCGGGGCGCCAATGAGCA
CAGCCTGGGATACGGTGTGG

Product: putative glycosyl transferase

Products: NA

Alternate protein names: N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferase; GlcNAc-Ins-P N-acetylglucosaminyltransferase

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MLAVHTSPLAQPGIGDAGGMNVYVLQSALHLARRGIEVEIFTRATASADPPIVWVAPGVLVRNVVAGPFEGLDKYDLPTQ
LCAFAAGVLRAEAAHEPGYYDIVHSHYWLSGQVGWLARDRWAVPLVHTAHTLAAVKNAALADGDAAEPPLRSVGEQQVVD
EADRMIVNTDDEARQLISIHRADPAKIDVAHPGVDLDMFRPGDRRAARAALGLPLDGNVVAFVGRIQPLKAPDIVLRAAA
KLPQVRIVVAGGPSGSGLASPDGLVRLADELGITARVTFLPPQSRTNLATVFQAADLVAVPSYSESFGLVAVEAQACGTP
VVAAAVGGLPVAVRDGVTGTLVFGHNVGHWADAVDQLLRLSAGPQARAISRAAVVHAAQFSWDNTTDALLASYRRAIGDF
TATRQHRVRDLVATRKPRRWISRRGMGA

Sequences:

>Translated_428_residues
MLAVHTSPLAQPGIGDAGGMNVYVLQSALHLARRGIEVEIFTRATASADPPIVWVAPGVLVRNVVAGPFEGLDKYDLPTQ
LCAFAAGVLRAEAAHEPGYYDIVHSHYWLSGQVGWLARDRWAVPLVHTAHTLAAVKNAALADGDAAEPPLRSVGEQQVVD
EADRMIVNTDDEARQLISIHRADPAKIDVAHPGVDLDMFRPGDRRAARAALGLPLDGNVVAFVGRIQPLKAPDIVLRAAA
KLPQVRIVVAGGPSGSGLASPDGLVRLADELGITARVTFLPPQSRTNLATVFQAADLVAVPSYSESFGLVAVEAQACGTP
VVAAAVGGLPVAVRDGVTGTLVFGHNVGHWADAVDQLLRLSAGPQARAISRAAVVHAAQFSWDNTTDALLASYRRAIGDF
TATRQHRVRDLVATRKPRRWISRRGMGA
>Mature_428_residues
MLAVHTSPLAQPGIGDAGGMNVYVLQSALHLARRGIEVEIFTRATASADPPIVWVAPGVLVRNVVAGPFEGLDKYDLPTQ
LCAFAAGVLRAEAAHEPGYYDIVHSHYWLSGQVGWLARDRWAVPLVHTAHTLAAVKNAALADGDAAEPPLRSVGEQQVVD
EADRMIVNTDDEARQLISIHRADPAKIDVAHPGVDLDMFRPGDRRAARAALGLPLDGNVVAFVGRIQPLKAPDIVLRAAA
KLPQVRIVVAGGPSGSGLASPDGLVRLADELGITARVTFLPPQSRTNLATVFQAADLVAVPSYSESFGLVAVEAQACGTP
VVAAAVGGLPVAVRDGVTGTLVFGHNVGHWADAVDQLLRLSAGPQARAISRAAVVHAAQFSWDNTTDALLASYRRAIGDF
TATRQHRVRDLVATRKPRRWISRRGMGA

Specific function: Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2- acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family. MshA subfamily

Homologues:

Organism=Escherichia coli, GI1790061, Length=192, Percent_Identity=26.0416666666667, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSHA_MYCLB (B8ZT88)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002504214.1
- ProteinModelPortal:   B8ZT88
- SMR:   B8ZT88
- EnsemblBacteria:   EBMYCT00000085304
- GeneID:   7324757
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015521
- HOGENOM:   HBG726846
- OMA:   PLKAPDV
- ProtClustDB:   CLSK871811
- HAMAP:   MF_01695
- InterPro:   IPR001296
- InterPro:   IPR017814
- TIGRFAMs:   TIGR03449

Pfam domain/function: PF00534 Glycos_transf_1

EC number: =2.4.1.250

Molecular weight: Translated: 45292; Mature: 45292

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00039 DEAD_ATP_HELICASE

Important sites: BINDING 5-5 BINDING 19-19 BINDING 74-74 BINDING 107-107 BINDING 131-131 BINDING 151-151 BINDING 225-225 BINDING 230-230 BINDING 283-283 BINDING 305-305 BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAVHTSPLAQPGIGDAGGMNVYVLQSALHLARRGIEVEIFTRATASADPPIVWVAPGVL
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCHHH
VRNVVAGPFEGLDKYDLPTQLCAFAAGVLRAEAAHEPGYYDIVHSHYWLSGQVGWLARDR
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECEEEECCCCCEEECCC
WAVPLVHTAHTLAAVKNAALADGDAAEPPLRSVGEQQVVDEADRMIVNTDDEARQLISIH
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHCCEEECCCHHHHHHHHHH
RADPAKIDVAHPGVDLDMFRPGDRRAARAALGLPLDGNVVAFVGRIQPLKAPDIVLRAAA
CCCCCEEEEECCCCCEECCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
KLPQVRIVVAGGPSGSGLASPDGLVRLADELGITARVTFLPPQSRTNLATVFQAADLVAV
CCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCEEEE
PSYSESFGLVAVEAQACGTPVVAAAVGGLPVAVRDGVTGTLVFGHNVGHWADAVDQLLRL
CCCCCCCCEEEEEHHHCCCCHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHH
SAGPQARAISRAAVVHAAQFSWDNTTDALLASYRRAIGDFTATRQHRVRDLVATRKPRRW
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ISRRGMGA
HHHCCCCC
>Mature Secondary Structure
MLAVHTSPLAQPGIGDAGGMNVYVLQSALHLARRGIEVEIFTRATASADPPIVWVAPGVL
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCHHH
VRNVVAGPFEGLDKYDLPTQLCAFAAGVLRAEAAHEPGYYDIVHSHYWLSGQVGWLARDR
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECEEEECCCCCEEECCC
WAVPLVHTAHTLAAVKNAALADGDAAEPPLRSVGEQQVVDEADRMIVNTDDEARQLISIH
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHCCEEECCCHHHHHHHHHH
RADPAKIDVAHPGVDLDMFRPGDRRAARAALGLPLDGNVVAFVGRIQPLKAPDIVLRAAA
CCCCCEEEEECCCCCEECCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
KLPQVRIVVAGGPSGSGLASPDGLVRLADELGITARVTFLPPQSRTNLATVFQAADLVAV
CCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCEEEE
PSYSESFGLVAVEAQACGTPVVAAAVGGLPVAVRDGVTGTLVFGHNVGHWADAVDQLLRL
CCCCCCCCEEEEEHHHCCCCHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHH
SAGPQARAISRAAVVHAAQFSWDNTTDALLASYRRAIGDFTATRQHRVRDLVATRKPRRW
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ISRRGMGA
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA