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The map label for this gene is proC

Identifier: 221230789

GI number: 221230789

Start: 2905388

End: 2906272

Strand: Reverse

Name: proC

Synonym: MLBr_02430

Alternate gene names: 221230789

Gene position: 2906272-2905388 (Counterclockwise)

Preceding gene: 221230790

Following gene: 221230788

Centisome position: 88.93

GC content: 60.68

Gene sequence:

>885_bases
ATGCTTTCCAGCATGGCAAGAATCGCGATCATCGGTGGTGGCAGTATTGGTGAAGCGTTGTTGTCGGGGCTTCTGCGGGC
GGGGAGGCAGGTCAAAGACCTGGTGGTGGCCGAACGGATGCCTGATCGCGCCCGATACCTAGCGGACACTTACTCGGTGT
TGGTGACGTCAGTAACCGACGCGGTTGAGAACGCAATGTTTGTCGTTGTCGCTGTGAAGCCGACCGACGTTGAGTCAGTG
ATGGGGGACCTGGTTCAGGCAGCCGCAGTCGCGAATGACAGCGCTGAGCAAGTGTTAGTCACCGTGGCTGCTGGTGTTAC
GATCACCTATCTCGAGTCCAAGCTTCCGGCTGGTACGCCGGTGGTTCGTGCCATGCCGAACGCAGCCGCGTTGGTGGGCG
CGGGGGTTACCGTGTTGGCCAAGGGGCGTTTCGTTACTGGACAACAGTTCGAGGATGTTTTGGCCATGTTCGACGCGGTG
GGTGGCGTTCTGACGGTTCCTGAGTCGCAAATGGATGCGGTTACCGCGGTATCGGGTTCAGGCCCGGCATATTTCTTCTT
GCTGGTCGAAGCTCTGGTGGATGCCGGCGTCGCGGTGGGTCTGACTCGTCAGGTGGCTACCGAACTGGCCGCGCAGACCA
TGGCGGGGTCCGCGGCAATGCTACTGGAGCGTATGGATCAAGATCGGCATTCAGCCGAGGTGGCGCCTCTGGGCGCGCAG
GTGGATGTCCCGGCGGCGCAACTACGCGCCACGATTACCTCGCCTGGTGGTACTACTGCCGCTGCGCTGCGCGAACTCGA
ACGTGGCGGACTGCGGATGGTGGTCGACGCAGCGGTTCAGGCCGCTAAAATCCGGTCTGAACAGCTCAGAATTACATCGG
AATAA

Upstream 100 bases:

>100_bases
TCACATCGTCGTCGACCAAGTTGTCCGTGTCGTGGCGCAGTTGTGCTACCTGGCGTTTGGTACTTCTGCCCGGTAGTACG
GTCGAGCCGAGCTGTCTGGG

Downstream 100 bases:

>100_bases
TTCAAGATTTTTGAACTGATTTCCCCCATCAGTACCAGTAACCCCACTAGTACCGCTATCCTCTTTTATTGTATGCACGT
GTGGGTGCCAGCGGAGGGGA

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase; P5CR

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLSSMARIAIIGGGSIGEALLSGLLRAGRQVKDLVVAERMPDRARYLADTYSVLVTSVTDAVENAMFVVVAVKPTDVESV
MGDLVQAAAVANDSAEQVLVTVAAGVTITYLESKLPAGTPVVRAMPNAAALVGAGVTVLAKGRFVTGQQFEDVLAMFDAV
GGVLTVPESQMDAVTAVSGSGPAYFFLLVEALVDAGVAVGLTRQVATELAAQTMAGSAAMLLERMDQDRHSAEVAPLGAQ
VDVPAAQLRATITSPGGTTAAALRELERGGLRMVVDAAVQAAKIRSEQLRITSE

Sequences:

>Translated_294_residues
MLSSMARIAIIGGGSIGEALLSGLLRAGRQVKDLVVAERMPDRARYLADTYSVLVTSVTDAVENAMFVVVAVKPTDVESV
MGDLVQAAAVANDSAEQVLVTVAAGVTITYLESKLPAGTPVVRAMPNAAALVGAGVTVLAKGRFVTGQQFEDVLAMFDAV
GGVLTVPESQMDAVTAVSGSGPAYFFLLVEALVDAGVAVGLTRQVATELAAQTMAGSAAMLLERMDQDRHSAEVAPLGAQ
VDVPAAQLRATITSPGGTTAAALRELERGGLRMVVDAAVQAAKIRSEQLRITSE
>Mature_294_residues
MLSSMARIAIIGGGSIGEALLSGLLRAGRQVKDLVVAERMPDRARYLADTYSVLVTSVTDAVENAMFVVVAVKPTDVESV
MGDLVQAAAVANDSAEQVLVTVAAGVTITYLESKLPAGTPVVRAMPNAAALVGAGVTVLAKGRFVTGQQFEDVLAMFDAV
GGVLTVPESQMDAVTAVSGSGPAYFFLLVEALVDAGVAVGLTRQVATELAAQTMAGSAAMLLERMDQDRHSAEVAPLGAQ
VDVPAAQLRATITSPGGTTAAALRELERGGLRMVVDAAVQAAKIRSEQLRITSE

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family

Homologues:

Organism=Homo sapiens, GI198041662, Length=284, Percent_Identity=32.7464788732394, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI21361454, Length=293, Percent_Identity=34.1296928327645, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI24797097, Length=293, Percent_Identity=34.1296928327645, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI24797095, Length=293, Percent_Identity=34.1296928327645, Blast_Score=124, Evalue=8e-29,
Organism=Escherichia coli, GI1786585, Length=285, Percent_Identity=34.0350877192982, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17569021, Length=284, Percent_Identity=35.9154929577465, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17540664, Length=293, Percent_Identity=31.3993174061433, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320861, Length=307, Percent_Identity=28.6644951140065, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21358587, Length=291, Percent_Identity=34.7079037800687, Blast_Score=151, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24648116, Length=291, Percent_Identity=32.9896907216495, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24647700, Length=185, Percent_Identity=38.9189189189189, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): P5CR_MYCLE (P46725)

Other databases:

- EMBL:   U00018
- EMBL:   AL583925
- PIR:   S72897
- RefSeq:   NP_302575.1
- ProteinModelPortal:   P46725
- SMR:   P46725
- EnsemblBacteria:   EBMYCT00000027960
- GeneID:   908565
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2430
- NMPDR:   fig|272631.1.peg.1447
- Leproma:   ML2430
- GeneTree:   EBGT00050000017740
- HOGENOM:   HBG726602
- OMA:   AMKPKDV
- ProtClustDB:   PRK11880
- BioCyc:   MLEP272631:ML2430-MONOMER
- BRENDA:   1.5.1.2
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11645
- PIRSF:   PIRSF000193
- TIGRFAMs:   TIGR00112

Pfam domain/function: PF03807 F420_oxidored; SSF48179 6DGDH_C_like

EC number: =1.5.1.2

Molecular weight: Translated: 30237; Mature: 30237

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSSMARIAIIGGGSIGEALLSGLLRAGRQVKDLVVAERMPDRARYLADTYSVLVTSVTD
CCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AVENAMFVVVAVKPTDVESVMGDLVQAAAVANDSAEQVLVTVAAGVTITYLESKLPAGTP
HHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHEEEHHCCCEEEEEEHHCCCCCCC
VVRAMPNAAALVGAGVTVLAKGRFVTGQQFEDVLAMFDAVGGVLTVPESQMDAVTAVSGS
HHHHCCCHHHHHHCCEEEEECCCEECCHHHHHHHHHHHHHCCEEECCCCHHHHEEEECCC
GPAYFFLLVEALVDAGVAVGLTRQVATELAAQTMAGSAAMLLERMDQDRHSAEVAPLGAQ
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHCCCCCCCCCC
VDVPAAQLRATITSPGGTTAAALRELERGGLRMVVDAAVQAAKIRSEQLRITSE
CCCCHHHHEEEEECCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MLSSMARIAIIGGGSIGEALLSGLLRAGRQVKDLVVAERMPDRARYLADTYSVLVTSVTD
CCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AVENAMFVVVAVKPTDVESVMGDLVQAAAVANDSAEQVLVTVAAGVTITYLESKLPAGTP
HHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHEEEHHCCCEEEEEEHHCCCCCCC
VVRAMPNAAALVGAGVTVLAKGRFVTGQQFEDVLAMFDAVGGVLTVPESQMDAVTAVSGS
HHHHCCCHHHHHHCCEEEEECCCEECCHHHHHHHHHHHHHCCEEECCCCHHHHEEEECCC
GPAYFFLLVEALVDAGVAVGLTRQVATELAAQTMAGSAAMLLERMDQDRHSAEVAPLGAQ
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHCCCCCCCCCC
VDVPAAQLRATITSPGGTTAAALRELERGGLRMVVDAAVQAAKIRSEQLRITSE
CCCCHHHHEEEEECCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002