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The map label for this gene is argF [H]

Identifier: 221230217

GI number: 221230217

Start: 1692068

End: 1693030

Strand: Direct

Name: argF [H]

Synonym: MLBr_01410

Alternate gene names: 221230217

Gene position: 1692068-1693030 (Clockwise)

Preceding gene: 221230216

Following gene: 221230218

Centisome position: 51.78

GC content: 62.62

Gene sequence:

>963_bases
GTGGGGGGCGCTTCCCGCTTGCGGGGAAGAGGAGCGGCGCAGTTGACTAGGCATTTTTTGCGTGATGACGATCTGTCGCC
GACTGAACAGGCCGACATTCTGGCGCTCGCTGCAGATCTGAAGAAGGCCCCGTTCAGCCGCCGTCCTCTTGCGGGCCCGC
GTGGGGTTGCGGTCATCTTCGACAAGAACTCCACGCGAACCCGGTTCTCATTCGATATAGGCATTGCGCAGCTCGGTGGG
CATGCCGTTGTTGTTGACGGGCTCAGCACTCAACTGGGTCGCGACGAGACCTTGCAAGACACGGCAAGGGTGTTGTCTGG
CTACGTCGATGCCATCGTCTGGCGGACCTTCGGGCAACACCGGCTAGATGCCATGGCCGCTACCGCGACGGTGCCGGTGG
TCAATGCGCTGTCCGACGAATTCCATCCGTGCCAAGTGCTGGCCGATCTGCAGACGATCGCCGAGCGCAAGGGATCATTG
AACGGCTTGCGACTGTCCTACTTCGGCGATGGTGCCAACAACATGGCTCATTCGCTTATGCTCGGTGCAGTCACCGCAGG
TGTCCACGTCACCGTCGCTACCCCAGTAGGTTTTACGCCGGATCCGTCGGTGCTGGCCGCGGCTAAGAAGCGTGCGGAGG
CGACTGGCGCGTCGGTGACCGTGACTGTCGACGCGGACACAGCCGCCGCGGGCGCCGATGTCCTGGTCACCGACACCTGG
ACGTCGATGGGACAGGAGAACGACGGGCTGGACCGGGTCAAGCCGTTTTGGCCGTTCCAGGTCAACGCCCGACTCGTGGG
GCTGGCCGACTCGGAAGCTATCGTGCTGCACTGCCTTCCAGCTCATCGTGGTGACGAGATCACCAACGAGGTAATGGACG
GACCGACCAGCGCGATCTGGGATGAGGCCGAAAACCGGCTGCACGCGCAGAAGGCACTGTTGGTGTGGCTACTGGAACGG
TAA

Upstream 100 bases:

>100_bases
GACAGCTTCATCACTGCCTTGCCAGGCATTCTGGATGCCTCCATAGCTGAGCTGGGAAAAAAGGCATGACAGCGCCGGTG
ACGATGCAGAGAGAAACGAT

Downstream 100 bases:

>100_bases
CGATGACCCACGGCGCTTCGAAGACCACACCCGAGACCACTCGGGCGGGTCGACAGGCCCGTATCGTGGCGATCCTGTCA
TCGACGTCGGTGCGCAGTCA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MGGASRLRGRGAAQLTRHFLRDDDLSPTEQADILALAADLKKAPFSRRPLAGPRGVAVIFDKNSTRTRFSFDIGIAQLGG
HAVVVDGLSTQLGRDETLQDTARVLSGYVDAIVWRTFGQHRLDAMAATATVPVVNALSDEFHPCQVLADLQTIAERKGSL
NGLRLSYFGDGANNMAHSLMLGAVTAGVHVTVATPVGFTPDPSVLAAAKKRAEATGASVTVTVDADTAAAGADVLVTDTW
TSMGQENDGLDRVKPFWPFQVNARLVGLADSEAIVLHCLPAHRGDEITNEVMDGPTSAIWDEAENRLHAQKALLVWLLER

Sequences:

>Translated_320_residues
MGGASRLRGRGAAQLTRHFLRDDDLSPTEQADILALAADLKKAPFSRRPLAGPRGVAVIFDKNSTRTRFSFDIGIAQLGG
HAVVVDGLSTQLGRDETLQDTARVLSGYVDAIVWRTFGQHRLDAMAATATVPVVNALSDEFHPCQVLADLQTIAERKGSL
NGLRLSYFGDGANNMAHSLMLGAVTAGVHVTVATPVGFTPDPSVLAAAKKRAEATGASVTVTVDADTAAAGADVLVTDTW
TSMGQENDGLDRVKPFWPFQVNARLVGLADSEAIVLHCLPAHRGDEITNEVMDGPTSAIWDEAENRLHAQKALLVWLLER
>Mature_319_residues
GGASRLRGRGAAQLTRHFLRDDDLSPTEQADILALAADLKKAPFSRRPLAGPRGVAVIFDKNSTRTRFSFDIGIAQLGGH
AVVVDGLSTQLGRDETLQDTARVLSGYVDAIVWRTFGQHRLDAMAATATVPVVNALSDEFHPCQVLADLQTIAERKGSLN
GLRLSYFGDGANNMAHSLMLGAVTAGVHVTVATPVGFTPDPSVLAAAKKRAEATGASVTVTVDADTAAAGADVLVTDTWT
SMGQENDGLDRVKPFWPFQVNARLVGLADSEAIVLHCLPAHRGDEITNEVMDGPTSAIWDEAENRLHAQKALLVWLLER

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=41.9672131147541, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=27.0096463022508, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=34.9544072948328, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1786469, Length=324, Percent_Identity=35.8024691358025, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI2367364, Length=317, Percent_Identity=32.4921135646688, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI48994908, Length=349, Percent_Identity=27.5071633237822, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=284, Percent_Identity=28.8732394366197, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322373, Length=312, Percent_Identity=35.2564102564103, Blast_Score=181, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6322331, Length=183, Percent_Identity=32.2404371584699, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24642586, Length=310, Percent_Identity=27.741935483871, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 34124; Mature: 33993

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGASRLRGRGAAQLTRHFLRDDDLSPTEQADILALAADLKKAPFSRRPLAGPRGVAVIF
CCCCHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
DKNSTRTRFSFDIGIAQLGGHAVVVDGLSTQLGRDETLQDTARVLSGYVDAIVWRTFGQH
ECCCCCEEEEEEECHHHHCCCEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHH
RLDAMAATATVPVVNALSDEFHPCQVLADLQTIAERKGSLNGLRLSYFGDGANNMAHSLM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
LGAVTAGVHVTVATPVGFTPDPSVLAAAKKRAEATGASVTVTVDADTAAAGADVLVTDTW
HHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEECH
TSMGQENDGLDRVKPFWPFQVNARLVGLADSEAIVLHCLPAHRGDEITNEVMDGPTSAIW
HHCCCCCCCCCCCCCCCCEEECEEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCCHHHH
DEAENRLHAQKALLVWLLER
HHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
GGASRLRGRGAAQLTRHFLRDDDLSPTEQADILALAADLKKAPFSRRPLAGPRGVAVIF
CCCHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
DKNSTRTRFSFDIGIAQLGGHAVVVDGLSTQLGRDETLQDTARVLSGYVDAIVWRTFGQH
ECCCCCEEEEEEECHHHHCCCEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHH
RLDAMAATATVPVVNALSDEFHPCQVLADLQTIAERKGSLNGLRLSYFGDGANNMAHSLM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
LGAVTAGVHVTVATPVGFTPDPSVLAAAKKRAEATGASVTVTVDADTAAAGADVLVTDTW
HHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEECH
TSMGQENDGLDRVKPFWPFQVNARLVGLADSEAIVLHCLPAHRGDEITNEVMDGPTSAIW
HHCCCCCCCCCCCCCCCCEEECEEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCCHHHH
DEAENRLHAQKALLVWLLER
HHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]