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The map label for this gene is pheS [H]

Identifier: 221230211

GI number: 221230211

Start: 1680970

End: 1682022

Strand: Direct

Name: pheS [H]

Synonym: MLBr_01401

Alternate gene names: 221230211

Gene position: 1680970-1682022 (Clockwise)

Preceding gene: 221230210

Following gene: 221230212

Centisome position: 51.44

GC content: 60.97

Gene sequence:

>1053_bases
GTGGGTGATCAACCCGTTGATCTGTCGATGTTGTCGTCGGAAGCACTGGCCAAGGCAGTTAACACCGCCCGCCAAGCCAT
CGCACTTGCCGTCAATCTCGACGCGTTGGCGCTCATCAAGGCCGAGCACCTCGGTGACCGCTCGCCGCTGGCGCTCGCAC
GACAAGCGCTTGCTAGCCTGCCCAAGATTGACCGGGCCGCCGTCGGCAAGCAGGTCAACACGGCCCGCAGCGAGGTCCAG
CGTGACTACGACGAACGACTGGCGACGTTGCAAGCCGAGCGCGACGCGGCCGTGCTGGTCACCGAACGTATCGATGTCAC
TCTGCCTTCAACAAGGCAGCCGACCGGAGCACGGCACCCGATAACGATATTGGCCGAGCATATCGCCGATACCTTCATTG
CGATGGGATGGGAACTGGTCGACGGACCCGAAGTCGAGACTGAGCAGTTTAACTTTGACGCTCTTAACTTTCCTGCCGAC
CACCCCGCACGCAGTGAACAGGACACCTTTTATATTGCGCCGGACGGTTCACGGCAGCTACTGCGAACCCATACTTCACC
AGTGCAGGTGCGGACTTTGCTCGAGCGTGAGCTGCCGGTCTACGTCATCTCGATCGGTCGAGCCTTCCGCACCGACGAAG
TCGATGCCACCCACACGCCCGTCTTCCATCAAGTCGAAGGCCTGGCAGTGGACCGCGGTCTTTCCATGGCGCATCTGCGG
GGAACGTTGGACATCTTCGCACGCGCGGAGTTCGGGCCGTCGACGCGCACCCGGATCCGGCCGCACTTCTTCCCATTCAC
CGAACCGTCGGCCGAGGTCGATGTATGGTTTGCGAGCAAAAAGGATGGCGCTGACTGGGTGGAATGGGGCGGTTGCGGAA
TGGTGCATCCAAACGTGTTGCGGGCAACAGGAATTGACCCGGATATCTACTCCGGTTTCGCATTCGGGATGGGCCTGGAA
CGCACCCTGCAGTTCCGCAACAGCATTCCCAACATGCGCGATATGGTCGAAGGGGACGTTCGGTTTTCGTTGCCGTTCGG
GGTGGGGGCTTGA

Upstream 100 bases:

>100_bases
GCCGATGCAGCGGACTGAAGAGAAGCCGCGCAAGTCCAGCCCAATTCAGCGCCGATGCGGGCAGTCCGACCAGCTTCGTC
AGCAAGGAGAGTGTCGCCGC

Downstream 100 bases:

>100_bases
TGCGCGTTCCGTATAGTTGGCTGTGCGAAGTGGTTACGGTTGGTGCACCGGATTGGGATGTTTCCGCGAGCGATCTCAAG
CAGACGCTGGTACGCATCGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MGDQPVDLSMLSSEALAKAVNTARQAIALAVNLDALALIKAEHLGDRSPLALARQALASLPKIDRAAVGKQVNTARSEVQ
RDYDERLATLQAERDAAVLVTERIDVTLPSTRQPTGARHPITILAEHIADTFIAMGWELVDGPEVETEQFNFDALNFPAD
HPARSEQDTFYIAPDGSRQLLRTHTSPVQVRTLLERELPVYVISIGRAFRTDEVDATHTPVFHQVEGLAVDRGLSMAHLR
GTLDIFARAEFGPSTRTRIRPHFFPFTEPSAEVDVWFASKKDGADWVEWGGCGMVHPNVLRATGIDPDIYSGFAFGMGLE
RTLQFRNSIPNMRDMVEGDVRFSLPFGVGA

Sequences:

>Translated_350_residues
MGDQPVDLSMLSSEALAKAVNTARQAIALAVNLDALALIKAEHLGDRSPLALARQALASLPKIDRAAVGKQVNTARSEVQ
RDYDERLATLQAERDAAVLVTERIDVTLPSTRQPTGARHPITILAEHIADTFIAMGWELVDGPEVETEQFNFDALNFPAD
HPARSEQDTFYIAPDGSRQLLRTHTSPVQVRTLLERELPVYVISIGRAFRTDEVDATHTPVFHQVEGLAVDRGLSMAHLR
GTLDIFARAEFGPSTRTRIRPHFFPFTEPSAEVDVWFASKKDGADWVEWGGCGMVHPNVLRATGIDPDIYSGFAFGMGLE
RTLQFRNSIPNMRDMVEGDVRFSLPFGVGA
>Mature_349_residues
GDQPVDLSMLSSEALAKAVNTARQAIALAVNLDALALIKAEHLGDRSPLALARQALASLPKIDRAAVGKQVNTARSEVQR
DYDERLATLQAERDAAVLVTERIDVTLPSTRQPTGARHPITILAEHIADTFIAMGWELVDGPEVETEQFNFDALNFPADH
PARSEQDTFYIAPDGSRQLLRTHTSPVQVRTLLERELPVYVISIGRAFRTDEVDATHTPVFHQVEGLAVDRGLSMAHLRG
TLDIFARAEFGPSTRTRIRPHFFPFTEPSAEVDVWFASKKDGADWVEWGGCGMVHPNVLRATGIDPDIYSGFAFGMGLER
TLQFRNSIPNMRDMVEGDVRFSLPFGVGA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=275, Percent_Identity=30.5454545454545, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI5729820, Length=275, Percent_Identity=29.4545454545455, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788007, Length=331, Percent_Identity=45.619335347432, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=30.6569343065693, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=30.6569343065693, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=310, Percent_Identity=29.6774193548387, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=369, Percent_Identity=28.1842818428184, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6325304, Length=285, Percent_Identity=27.719298245614, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18858079, Length=271, Percent_Identity=31.7343173431734, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17137424, Length=281, Percent_Identity=29.1814946619217, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38566; Mature: 38435

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDQPVDLSMLSSEALAKAVNTARQAIALAVNLDALALIKAEHLGDRSPLALARQALASL
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCHHEEHHHHHCCCCCHHHHHHHHHHHC
PKIDRAAVGKQVNTARSEVQRDYDERLATLQAERDAAVLVTERIDVTLPSTRQPTGARHP
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCH
ITILAEHIADTFIAMGWELVDGPEVETEQFNFDALNFPADHPARSEQDTFYIAPDGSRQL
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCHHH
LRTHTSPVQVRTLLERELPVYVISIGRAFRTDEVDATHTPVFHQVEGLAVDRGLSMAHLR
HHHCCCHHHHHHHHHCCCCEEEEEECCEECCCCCCCCCCCHHHHHCCEECCCCCCHHHHH
GTLDIFARAEFGPSTRTRIRPHFFPFTEPSAEVDVWFASKKDGADWVEWGGCGMVHPNVL
HHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCE
RATGIDPDIYSGFAFGMGLERTLQFRNSIPNMRDMVEGDVRFSLPFGVGA
EECCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCC
>Mature Secondary Structure 
GDQPVDLSMLSSEALAKAVNTARQAIALAVNLDALALIKAEHLGDRSPLALARQALASL
CCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCHHEEHHHHHCCCCCHHHHHHHHHHHC
PKIDRAAVGKQVNTARSEVQRDYDERLATLQAERDAAVLVTERIDVTLPSTRQPTGARHP
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCH
ITILAEHIADTFIAMGWELVDGPEVETEQFNFDALNFPADHPARSEQDTFYIAPDGSRQL
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCHHH
LRTHTSPVQVRTLLERELPVYVISIGRAFRTDEVDATHTPVFHQVEGLAVDRGLSMAHLR
HHHCCCHHHHHHHHHCCCCEEEEEECCEECCCCCCCCCCCHHHHHCCEECCCCCCHHHHH
GTLDIFARAEFGPSTRTRIRPHFFPFTEPSAEVDVWFASKKDGADWVEWGGCGMVHPNVL
HHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCE
RATGIDPDIYSGFAFGMGLERTLQFRNSIPNMRDMVEGDVRFSLPFGVGA
EECCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]