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The map label for this gene is trmI [H]

Identifier: 221230158

GI number: 221230158

Start: 1566134

End: 1566979

Strand: Direct

Name: trmI [H]

Synonym: MLBr_01313

Alternate gene names: 221230158

Gene position: 1566134-1566979 (Clockwise)

Preceding gene: 221230156

Following gene: 221230161

Centisome position: 47.92

GC content: 63.12

Gene sequence:

>846_bases
GTGTCCGCAACTGGCCCGTTCACCGTTGGCGAGCGCGTCCAGCTCACCGACACTAAAGGCCGCCACTACACGATGTCGCT
CACCCCTGGTGGAGAATTCCACACTCATCGCGGCTCGATTGCCCACGATGGGATCATCGGCTTGGAACAGGGCAGCGTGG
TGAAATCCAGCACCGGTGCCCTGTTCTTGGTCTTGCGCCCGTTGCTGATCGATTACGTGATGTCGATGCCGCGCGGGCCA
CAGGTGATCTACCCCAAGGATGCCGCTCAGATCGTGCACGAAGGTGATATCTTTCCCGGTGCCCGGGTACTGGAGGCCGG
GGCCGGATCCGGTGCGCTGACGTGCTCGCTGCTCCGAGCGGTCGGGCCGCAGGGGCAGGTGATTTCCTACGAACTGCGCA
CCGATCACGCTGAACACGCTCGTCGCAATGTGTCTAACTTCTTTTGTGTCTCCGGCGCCGAAGGCCGACTATCGGACAAT
TGGCAGCTCATCGTTAGTGATGTTGCGAATTCAGAGTTGCCCGACGGTTCCGTCGACCGGGTGGTGTTAGACATGCTGGC
GCCGTGGGAGGTGCTGGACACAGTCTCGCGGCTGGTGATCGCCGGCGGCGTATTGGTGATCTACGTGGCCACCGTCACCC
AGCTGTCGCGGGCTGTGGAGGCTCTGCGGGTTCAGCAATGTTGGGTTGAACCGCGGGCGTGGGAAACGCTTCAGAGGGGT
TGGAACGTGGTTGGTCTGGCGGTTCGGCCAGAGCATTTGATGCGTGGGCACACCGCGTTCCTGATTTTGGCCCGCCGGCT
GGCGCCCGGCGCTGTCGCGCCGGCGCCGTGGGGTCGCAAACGGTGA

Upstream 100 bases:

>100_bases
TCGAAGTGGCTTCGGCTGGTCGATCATGACGGCAGCCTAGGGCAGGACACCGACAACCCCAAGCACCGGCGGTGGCGAGT
CGGCTGGCACGCTAGACAGC

Downstream 100 bases:

>100_bases
AAACGGTGAGGAGCGTGACAGCTAGTGATGATCGCGAGTGCGACGAAGCCGACCGTAGCGGGTTATCACTATCGGATTTA
GCACTATCGACCGTACCGGA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m1A58)-methyltransferase; tRNA(m1A58)MTase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSATGPFTVGERVQLTDTKGRHYTMSLTPGGEFHTHRGSIAHDGIIGLEQGSVVKSSTGALFLVLRPLLIDYVMSMPRGP
QVIYPKDAAQIVHEGDIFPGARVLEAGAGSGALTCSLLRAVGPQGQVISYELRTDHAEHARRNVSNFFCVSGAEGRLSDN
WQLIVSDVANSELPDGSVDRVVLDMLAPWEVLDTVSRLVIAGGVLVIYVATVTQLSRAVEALRVQQCWVEPRAWETLQRG
WNVVGLAVRPEHLMRGHTAFLILARRLAPGAVAPAPWGRKR

Sequences:

>Translated_281_residues
MSATGPFTVGERVQLTDTKGRHYTMSLTPGGEFHTHRGSIAHDGIIGLEQGSVVKSSTGALFLVLRPLLIDYVMSMPRGP
QVIYPKDAAQIVHEGDIFPGARVLEAGAGSGALTCSLLRAVGPQGQVISYELRTDHAEHARRNVSNFFCVSGAEGRLSDN
WQLIVSDVANSELPDGSVDRVVLDMLAPWEVLDTVSRLVIAGGVLVIYVATVTQLSRAVEALRVQQCWVEPRAWETLQRG
WNVVGLAVRPEHLMRGHTAFLILARRLAPGAVAPAPWGRKR
>Mature_280_residues
SATGPFTVGERVQLTDTKGRHYTMSLTPGGEFHTHRGSIAHDGIIGLEQGSVVKSSTGALFLVLRPLLIDYVMSMPRGPQ
VIYPKDAAQIVHEGDIFPGARVLEAGAGSGALTCSLLRAVGPQGQVISYELRTDHAEHARRNVSNFFCVSGAEGRLSDNW
QLIVSDVANSELPDGSVDRVVLDMLAPWEVLDTVSRLVIAGGVLVIYVATVTQLSRAVEALRVQQCWVEPRAWETLQRGW
NVVGLAVRPEHLMRGHTAFLILARRLAPGAVAPAPWGRKR

Specific function: Catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in tRNA. Is required for cell growth at extreme temperatures [H]

COG id: COG2519

COG function: function code J; tRNA(1-methyladenosine) methyltransferase and related methyltransferases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmI family [H]

Homologues:

Organism=Homo sapiens, GI222831587, Length=248, Percent_Identity=32.6612903225806, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI123173772, Length=230, Percent_Identity=27.3913043478261, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17509459, Length=216, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24650257, Length=223, Percent_Identity=32.2869955156951, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014816 [H]

Pfam domain/function: PF08704 GCD14 [H]

EC number: =2.1.1.36 [H]

Molecular weight: Translated: 30455; Mature: 30324

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATGPFTVGERVQLTDTKGRHYTMSLTPGGEFHTHRGSIAHDGIIGLEQGSVVKSSTGA
CCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEECCCCC
LFLVLRPLLIDYVMSMPRGPQVIYPKDAAQIVHEGDIFPGARVLEAGAGSGALTCSLLRA
HHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCCCCCHHHEECCCCCCHHHHHHHHH
VGPQGQVISYELRTDHAEHARRNVSNFFCVSGAEGRLSDNWQLIVSDVANSELPDGSVDR
HCCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEHHCCCCCCCCCHHH
VVLDMLAPWEVLDTVSRLVIAGGVLVIYVATVTQLSRAVEALRVQQCWVEPRAWETLQRG
HHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
WNVVGLAVRPEHLMRGHTAFLILARRLAPGAVAPAPWGRKR
CCEEEEEECHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SATGPFTVGERVQLTDTKGRHYTMSLTPGGEFHTHRGSIAHDGIIGLEQGSVVKSSTGA
CCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEECCCCC
LFLVLRPLLIDYVMSMPRGPQVIYPKDAAQIVHEGDIFPGARVLEAGAGSGALTCSLLRA
HHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCCCCCHHHEECCCCCCHHHHHHHHH
VGPQGQVISYELRTDHAEHARRNVSNFFCVSGAEGRLSDNWQLIVSDVANSELPDGSVDR
HCCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEHHCCCCCCCCCHHH
VVLDMLAPWEVLDTVSRLVIAGGVLVIYVATVTQLSRAVEALRVQQCWVEPRAWETLQRG
HHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
WNVVGLAVRPEHLMRGHTAFLILARRLAPGAVAPAPWGRKR
CCEEEEEECHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA