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The map label for this gene is cysS2 [H]

Identifier: 221230150

GI number: 221230150

Start: 1550253

End: 1551407

Strand: Direct

Name: cysS2 [H]

Synonym: MLBr_01302

Alternate gene names: 221230150

Gene position: 1550253-1551407 (Clockwise)

Preceding gene: 221230149

Following gene: 221230154

Centisome position: 47.44

GC content: 61.47

Gene sequence:

>1155_bases
GTGTCCGCTGCTGTTGCGCCCACTTGCAAGGCGACCATGTACGTCTGCGGGATCACTCCGTATGACGCCACTCATCTGGG
CCACGCCGCAACCTATTTGGCATTCGACCTGATCCACCGACTATGGCTGGACTTGGGTCACGAGGTGCATTATGTGCAGA
ACGTCACCGATGTTGACGATCCGTTGTTTGAACGCGCTGATCGTAACGGGGTCGATTGGCGCGACCTCGCCGAACGCGAG
GTCGCGCTGTTCCGCGATGATATGGCATCGTTGCGGATACTGCCGCCGCATGACTACGTGGCGGCGACTGAAACGATTGT
CGAGATCGTCGAACTAGTCGACAAGATGCTGGTTTCGGGGGCCGCGTATGTCATCGACGACGAGTACCCGGACATTTACT
TCCGAGCTGATGCAACGCTGCAGTTCGGCTACGAGTCAGGCTATGACCGAGACACCATGCTACGATTGTACGAGCAAAGC
GGCGGTGATCCGCGCCGGCCTGGAAAAAACGGCGAGCTTGACGCATTGCTGTGGCGGGCTGCACGACCCGGCGAGCCCAG
TTGGTCGTCACCGTTCGGTCCGGGACGGCCGGGCTGGCACGTCGAATGCGCGGCAATTGCGCTCAGCCGTATCGGCATCG
GCCTGGACATTCAGGGCGGGGGCAGCGACCTGATCTTTCCGCATCACGAGTTCACCGCGGCGCATGCTGAATGCGTCAGA
GGTGAACGGCGATTCGCCCGGCACTACGTGCACGCTGGGATGATCGGCTGGGACGAACACAAGATGTCTAAGAGCCGCGG
CAACCTGGTGCTGGTGTCGACGCTGCGCGCTCAAGGCGCCCCGCCCTCGGCTATCCGGCTGGGTCTGCTAGCCGGGCATT
ATCGTGCTGACAGGTTTTGGAGTTCGCAGCTGCTAGACGATGCTATCGCCCGGTTGCATCGCTGGCGCACCGCTGCCTCG
ATGCCCGCCGGTCCGGACGTCGCTGACGTTATTGCCCGGGTGCGCGGGTATTTGGCTGACGATCTTGACACTCCTAAAGC
GATTGCTGCATTGGATGGTTGGGTAACCGATGCGCTGGAGTACGGAGGCCACGATGCGGCGGCGCCAAAGTTGCTGGCAA
CCGCGATCGATGCGTTGCTGGGGGTGGACCTGTAA

Upstream 100 bases:

>100_bases
CAACGACATGCTGTCGTGGTCTTTCGCACAGGTTCCGGCGTTGCCGGGCCGCGGCCCGGAGCTCAGGCTGTACGACACCT
CTGATCGACAGGTACGTCCG

Downstream 100 bases:

>100_bases
TGGTCGCAGAGGCAAAGTGCGGTTTTCGGCCACGACATCGTTGCACGGCAAAGTCATGTTCATCACGGGTGGTGCCCAGG
TGAATCGGCGCACAGCTGGC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: L-Cys:GlcN-Ins ligase; Mycothiol ligase; MSH ligase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSAAVAPTCKATMYVCGITPYDATHLGHAATYLAFDLIHRLWLDLGHEVHYVQNVTDVDDPLFERADRNGVDWRDLAERE
VALFRDDMASLRILPPHDYVAATETIVEIVELVDKMLVSGAAYVIDDEYPDIYFRADATLQFGYESGYDRDTMLRLYEQS
GGDPRRPGKNGELDALLWRAARPGEPSWSSPFGPGRPGWHVECAAIALSRIGIGLDIQGGGSDLIFPHHEFTAAHAECVR
GERRFARHYVHAGMIGWDEHKMSKSRGNLVLVSTLRAQGAPPSAIRLGLLAGHYRADRFWSSQLLDDAIARLHRWRTAAS
MPAGPDVADVIARVRGYLADDLDTPKAIAALDGWVTDALEYGGHDAAAPKLLATAIDALLGVDL

Sequences:

>Translated_384_residues
MSAAVAPTCKATMYVCGITPYDATHLGHAATYLAFDLIHRLWLDLGHEVHYVQNVTDVDDPLFERADRNGVDWRDLAERE
VALFRDDMASLRILPPHDYVAATETIVEIVELVDKMLVSGAAYVIDDEYPDIYFRADATLQFGYESGYDRDTMLRLYEQS
GGDPRRPGKNGELDALLWRAARPGEPSWSSPFGPGRPGWHVECAAIALSRIGIGLDIQGGGSDLIFPHHEFTAAHAECVR
GERRFARHYVHAGMIGWDEHKMSKSRGNLVLVSTLRAQGAPPSAIRLGLLAGHYRADRFWSSQLLDDAIARLHRWRTAAS
MPAGPDVADVIARVRGYLADDLDTPKAIAALDGWVTDALEYGGHDAAAPKLLATAIDALLGVDL
>Mature_383_residues
SAAVAPTCKATMYVCGITPYDATHLGHAATYLAFDLIHRLWLDLGHEVHYVQNVTDVDDPLFERADRNGVDWRDLAEREV
ALFRDDMASLRILPPHDYVAATETIVEIVELVDKMLVSGAAYVIDDEYPDIYFRADATLQFGYESGYDRDTMLRLYEQSG
GDPRRPGKNGELDALLWRAARPGEPSWSSPFGPGRPGWHVECAAIALSRIGIGLDIQGGGSDLIFPHHEFTAAHAECVRG
ERRFARHYVHAGMIGWDEHKMSKSRGNLVLVSTLRAQGAPPSAIRLGLLAGHYRADRFWSSQLLDDAIARLHRWRTAASM
PAGPDVADVIARVRGYLADDLDTPKAIAALDGWVTDALEYGGHDAAAPKLLATAIDALLGVDL

Specific function: Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins [H]

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=371, Percent_Identity=32.8840970350404, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI303304987, Length=239, Percent_Identity=32.2175732217573, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI21269877, Length=239, Percent_Identity=32.2175732217573, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI10835051, Length=239, Percent_Identity=32.2175732217573, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI62240992, Length=239, Percent_Identity=32.2175732217573, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1786737, Length=383, Percent_Identity=36.8146214099217, Blast_Score=237, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17509795, Length=249, Percent_Identity=30.5220883534137, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17509797, Length=249, Percent_Identity=30.5220883534137, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324082, Length=356, Percent_Identity=28.0898876404494, Blast_Score=135, Evalue=8e-33,
Organism=Drosophila melanogaster, GI19922218, Length=366, Percent_Identity=34.6994535519126, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI19922364, Length=226, Percent_Identity=32.7433628318584, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR017812
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF01406 tRNA-synt_1e [H]

EC number: =6.3.1.13 [H]

Molecular weight: Translated: 42222; Mature: 42091

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAVAPTCKATMYVCGITPYDATHLGHAATYLAFDLIHRLWLDLGHEVHYVQNVTDVDD
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCCCCC
PLFERADRNGVDWRDLAEREVALFRDDMASLRILPPHDYVAATETIVEIVELVDKMLVSG
HHHHHCCCCCCCHHHHHHHHHHHHHHCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
AAYVIDDEYPDIYFRADATLQFGYESGYDRDTMLRLYEQSGGDPRRPGKNGELDALLWRA
CEEEEECCCCCEEEEECCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHC
ARPGEPSWSSPFGPGRPGWHVECAAIALSRIGIGLDIQGGGSDLIFPHHEFTAAHAECVR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHH
GERRFARHYVHAGMIGWDEHKMSKSRGNLVLVSTLRAQGAPPSAIRLGLLAGHYRADRFW
HHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHH
SSQLLDDAIARLHRWRTAASMPAGPDVADVIARVRGYLADDLDTPKAIAALDGWVTDALE
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
YGGHDAAAPKLLATAIDALLGVDL
HCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAAVAPTCKATMYVCGITPYDATHLGHAATYLAFDLIHRLWLDLGHEVHYVQNVTDVDD
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCCCCC
PLFERADRNGVDWRDLAEREVALFRDDMASLRILPPHDYVAATETIVEIVELVDKMLVSG
HHHHHCCCCCCCHHHHHHHHHHHHHHCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
AAYVIDDEYPDIYFRADATLQFGYESGYDRDTMLRLYEQSGGDPRRPGKNGELDALLWRA
CEEEEECCCCCEEEEECCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHC
ARPGEPSWSSPFGPGRPGWHVECAAIALSRIGIGLDIQGGGSDLIFPHHEFTAAHAECVR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHH
GERRFARHYVHAGMIGWDEHKMSKSRGNLVLVSTLRAQGAPPSAIRLGLLAGHYRADRFW
HHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHH
SSQLLDDAIARLHRWRTAASMPAGPDVADVIARVRGYLADDLDTPKAIAALDGWVTDALE
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
YGGHDAAAPKLLATAIDALLGVDL
HCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]