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The map label for this gene is trpE

Identifier: 221230128

GI number: 221230128

Start: 1512163

End: 1513752

Strand: Direct

Name: trpE

Synonym: MLBr_01269

Alternate gene names: 221230128

Gene position: 1512163-1513752 (Clockwise)

Preceding gene: 221230127

Following gene: 221230129

Centisome position: 46.27

GC content: 62.2

Gene sequence:

>1590_bases
GTGCACGCCCACCTCGCCGCCACCACGTCACGTGAGGACTTTCGCCAGCTAGCTGTCGATCATCGGGTGGTTCCAGTGAC
CCGCAAAGTCTTGGCCGATAGCGAAACGCCGCTATCGGCATACCGGAAGCTCGCCGCCAATCGTCCGAGCACCTTCCTGC
TAGAGTCGGCCGAGAACGGCAGATCCTGGTCGCAGTGGTCGTTCATTGGCGTGGGTGCCCCATCGGCTTTGACAATTCGT
GACGGCGAAGCGGTGTGGCTGGGCACTGTACCGCAGGACGCGCCCACTGGCGGTGACCCCCTGCATGTCCTGCAGGCCAC
GCTCGAGCTACTTGCCACCGCGGCAATGCCCGGGTTGCCACCGCTATCGAGTGGTATGGTGGGCTTCTTCGCCTACGACA
TGGTGCGGCGCCTGGAGCGCCTGCCTGAACTGGCCTTAAATGATTTGCAGTTGCCCGATATGCTGCTGCTGTTGGCCACC
GATGTAGCGGCCGTTGACCACCACGAGGGCACGATCACCCTGATTGCCAACGCTGTGAACTGGAACGGTACCGATGAGCG
GGTAGATCAGGCCTACGACGATGCGATCGCGCGCTTGGACGTAATGACCGCAGCACTGGGCCAGCCGCTGCCATCGACCA
TTGCCACGTTCAGCCGGCCCGACCCTCGGCGCCGAGCGCAATGCACCATCGAGGAATACGGTGCGATCGTCGACCACCTC
GTCGACCAGATCGCGGCCGGTGAGGCCTTTCAAGTGGTGCCCTCGCAGCGCTTCGAGGTGGATACCGATGTCGATCCGAT
CGATGTCTATCGCATGCTGCGGGTCACCAACCCTAGCCCTTACATGTATCTGCTGCATGTGCCTAATAGCGATGGAGCAA
CTGGCTTTTCGATCGTTGGATCCAGCCCGGAGGCGTTGGTGACCGTCAAGGACGGTCGGGTGACGACGCATCCGATCGCT
GGAACTCGCTGGCGAGGCCAGACCGAAGAAGAGGATCAGCTACTGGAAAAAGAGTTGCTCGCCGACGAGAAGGAACGAGC
AGAGCACTTAATGCTTGTCGATCTCGGTCGCAACGACCTTGGTCGGGTCTGTACGCCAGGCACCGTACGTGTCGAGGATT
ACAGCCACGTTGAACGTTACAGTCACGTAATGCACATGGTGTCTACGGTGACCGGGCTGCTCGGTGAAGGCCGCACCGCC
CTGGACGCGGTGACCGCCTGCTTCCCTGCCGGCACGCTGTCGGGTGCCCCGAAGGTGCGGTCCATGGAGCTAATCGAGGA
AGTGGAGAAGACGCGCCGCGGCCTTTATGGTGGCGTGGTCGGTTACCTCGACTTCGCTGGTAACGCTGACTTCGCGATAG
CAATCCGTACAGCGCTGATGCGTGACGGCATCGCGTATGTCCAAGCCGGCGGTGGGGTAGTGGCCGACTCCAACGGGCCC
TACGAATACATCGAGGCGAGTAACAAGGCTCGAGCGGTGTTGAACGCGATCGCCGCCGCCGAGACGTTGACCTCTTTGGA
CTTCGGTGTTGCCCTCGCCCCTGGCCGCGTGGCGGCCAGGGGCGAGGCGGGCAATCAAGGGAGGCTGTGA

Upstream 100 bases:

>100_bases
CACTCAATCGCCCGCGCTTACGTCTGCACATCGGCACGCCATGTAGAACGGTAGTTTAGGGGCGTTCGGACGTGATAAGC
CCATCTGGAAGGATGAATCG

Downstream 100 bases:

>100_bases
TGGCTCCTGATATCAAGAGCGCCCGGGCCGGCCGGCTGACGATTCAAATAGCGCAGTTGTTGCTGGTGGTTGCTGCTGGC
GCATTGTGGATGGCGGCCCG

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MHAHLAATTSREDFRQLAVDHRVVPVTRKVLADSETPLSAYRKLAANRPSTFLLESAENGRSWSQWSFIGVGAPSALTIR
DGEAVWLGTVPQDAPTGGDPLHVLQATLELLATAAMPGLPPLSSGMVGFFAYDMVRRLERLPELALNDLQLPDMLLLLAT
DVAAVDHHEGTITLIANAVNWNGTDERVDQAYDDAIARLDVMTAALGQPLPSTIATFSRPDPRRRAQCTIEEYGAIVDHL
VDQIAAGEAFQVVPSQRFEVDTDVDPIDVYRMLRVTNPSPYMYLLHVPNSDGATGFSIVGSSPEALVTVKDGRVTTHPIA
GTRWRGQTEEEDQLLEKELLADEKERAEHLMLVDLGRNDLGRVCTPGTVRVEDYSHVERYSHVMHMVSTVTGLLGEGRTA
LDAVTACFPAGTLSGAPKVRSMELIEEVEKTRRGLYGGVVGYLDFAGNADFAIAIRTALMRDGIAYVQAGGGVVADSNGP
YEYIEASNKARAVLNAIAAAETLTSLDFGVALAPGRVAARGEAGNQGRL

Sequences:

>Translated_529_residues
MHAHLAATTSREDFRQLAVDHRVVPVTRKVLADSETPLSAYRKLAANRPSTFLLESAENGRSWSQWSFIGVGAPSALTIR
DGEAVWLGTVPQDAPTGGDPLHVLQATLELLATAAMPGLPPLSSGMVGFFAYDMVRRLERLPELALNDLQLPDMLLLLAT
DVAAVDHHEGTITLIANAVNWNGTDERVDQAYDDAIARLDVMTAALGQPLPSTIATFSRPDPRRRAQCTIEEYGAIVDHL
VDQIAAGEAFQVVPSQRFEVDTDVDPIDVYRMLRVTNPSPYMYLLHVPNSDGATGFSIVGSSPEALVTVKDGRVTTHPIA
GTRWRGQTEEEDQLLEKELLADEKERAEHLMLVDLGRNDLGRVCTPGTVRVEDYSHVERYSHVMHMVSTVTGLLGEGRTA
LDAVTACFPAGTLSGAPKVRSMELIEEVEKTRRGLYGGVVGYLDFAGNADFAIAIRTALMRDGIAYVQAGGGVVADSNGP
YEYIEASNKARAVLNAIAAAETLTSLDFGVALAPGRVAARGEAGNQGRL
>Mature_529_residues
MHAHLAATTSREDFRQLAVDHRVVPVTRKVLADSETPLSAYRKLAANRPSTFLLESAENGRSWSQWSFIGVGAPSALTIR
DGEAVWLGTVPQDAPTGGDPLHVLQATLELLATAAMPGLPPLSSGMVGFFAYDMVRRLERLPELALNDLQLPDMLLLLAT
DVAAVDHHEGTITLIANAVNWNGTDERVDQAYDDAIARLDVMTAALGQPLPSTIATFSRPDPRRRAQCTIEEYGAIVDHL
VDQIAAGEAFQVVPSQRFEVDTDVDPIDVYRMLRVTNPSPYMYLLHVPNSDGATGFSIVGSSPEALVTVKDGRVTTHPIA
GTRWRGQTEEEDQLLEKELLADEKERAEHLMLVDLGRNDLGRVCTPGTVRVEDYSHVERYSHVMHMVSTVTGLLGEGRTA
LDAVTACFPAGTLSGAPKVRSMELIEEVEKTRRGLYGGVVGYLDFAGNADFAIAIRTALMRDGIAYVQAGGGVVADSNGP
YEYIEASNKARAVLNAIAAAETLTSLDFGVALAPGRVAARGEAGNQGRL

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family

Homologues:

Organism=Escherichia coli, GI1787518, Length=381, Percent_Identity=42.5196850393701, Blast_Score=268, Evalue=8e-73,
Organism=Escherichia coli, GI1788114, Length=458, Percent_Identity=31.8777292576419, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1786809, Length=248, Percent_Identity=29.4354838709677, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082077, Length=201, Percent_Identity=26.3681592039801, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320935, Length=482, Percent_Identity=39.8340248962656, Blast_Score=306, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6324361, Length=401, Percent_Identity=23.6907730673317, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPE_MYCLE (Q9X7C5)

Other databases:

- EMBL:   AL049913
- EMBL:   AL583921
- PIR:   T45254
- RefSeq:   NP_301914.1
- ProteinModelPortal:   Q9X7C5
- SMR:   Q9X7C5
- EnsemblBacteria:   EBMYCT00000029079
- GeneID:   910378
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1269
- NMPDR:   fig|272631.1.peg.786
- Leproma:   ML1269
- GeneTree:   EBGT00050000015088
- HOGENOM:   HBG507440
- OMA:   PRFNGGL
- ProtClustDB:   PRK13571
- BioCyc:   MLEP272631:ML1269-MONOMER
- BRENDA:   4.1.3.27
- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236
- PRINTS:   PR00095
- TIGRFAMs:   TIGR00564

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =4.1.3.27

Molecular weight: Translated: 57032; Mature: 57032

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAHLAATTSREDFRQLAVDHRVVPVTRKVLADSETPLSAYRKLAANRPSTFLLESAENG
CCCCEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCC
RSWSQWSFIGVGAPSALTIRDGEAVWLGTVPQDAPTGGDPLHVLQATLELLATAAMPGLP
CCCCCEEEEEECCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PLSSGMVGFFAYDMVRRLERLPELALNDLQLPDMLLLLATDVAAVDHHEGTITLIANAVN
CCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHEECCCCEEEEEEECCC
WNGTDERVDQAYDDAIARLDVMTAALGQPLPSTIATFSRPDPRRRAQCTIEEYGAIVDHL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHCCCHHHHHHHHHHH
VDQIAAGEAFQVVPSQRFEVDTDVDPIDVYRMLRVTNPSPYMYLLHVPNSDGATGFSIVG
HHHHHCCCEEEECCCCCEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEC
SSPEALVTVKDGRVTTHPIAGTRWRGQTEEEDQLLEKELLADEKERAEHLMLVDLGRNDL
CCCCEEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCEEEEEECCCCCC
GRVCTPGTVRVEDYSHVERYSHVMHMVSTVTGLLGEGRTALDAVTACFPAGTLSGAPKVR
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHH
SMELIEEVEKTRRGLYGGVVGYLDFAGNADFAIAIRTALMRDGIAYVQAGGGVVADSNGP
HHHHHHHHHHHHHHHHCCHHHEEECCCCCCCHHHHHHHHHHCCCEEEECCCCEEECCCCC
YEYIEASNKARAVLNAIAAAETLTSLDFGVALAPGRVAARGEAGNQGRL
CHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCCCCCCCCC
>Mature Secondary Structure
MHAHLAATTSREDFRQLAVDHRVVPVTRKVLADSETPLSAYRKLAANRPSTFLLESAENG
CCCCEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCC
RSWSQWSFIGVGAPSALTIRDGEAVWLGTVPQDAPTGGDPLHVLQATLELLATAAMPGLP
CCCCCEEEEEECCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PLSSGMVGFFAYDMVRRLERLPELALNDLQLPDMLLLLATDVAAVDHHEGTITLIANAVN
CCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHEECCCCEEEEEEECCC
WNGTDERVDQAYDDAIARLDVMTAALGQPLPSTIATFSRPDPRRRAQCTIEEYGAIVDHL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHCCCHHHHHHHHHHH
VDQIAAGEAFQVVPSQRFEVDTDVDPIDVYRMLRVTNPSPYMYLLHVPNSDGATGFSIVG
HHHHHCCCEEEECCCCCEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEC
SSPEALVTVKDGRVTTHPIAGTRWRGQTEEEDQLLEKELLADEKERAEHLMLVDLGRNDL
CCCCEEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCEEEEEECCCCCC
GRVCTPGTVRVEDYSHVERYSHVMHMVSTVTGLLGEGRTALDAVTACFPAGTLSGAPKVR
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHH
SMELIEEVEKTRRGLYGGVVGYLDFAGNADFAIAIRTALMRDGIAYVQAGGGVVADSNGP
HHHHHHHHHHHHHHHHCCHHHEEECCCCCCCHHHHHHHHHHCCCEEEECCCCEEECCCCC
YEYIEASNKARAVLNAIAAAETLTSLDFGVALAPGRVAARGEAGNQGRL
CHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002