Click here to switch to the map view.

The map label for this gene is echA12

Identifier: 221230110

GI number: 221230110

Start: 1476575

End: 1477459

Strand: Direct

Name: echA12

Synonym: MLBr_01241

Alternate gene names: 221230110

Gene position: 1476575-1477459 (Clockwise)

Preceding gene: 221230106

Following gene: 221230111

Centisome position: 45.18

GC content: 58.53

Gene sequence:

>885_bases
GTGAGTCAGACTGATGCATCGTGCACTATTGCAGAGCTTCCCTATAGATCCGTCACGGACTTGGTCGTGCTGGATTTTCC
GCGACCTGAGGTCGCGTTGATCACTCTTAATCGGCCCGGCCGGATGAATTCCATGGCTCTCGACCTTATGAAGTCGCTCA
AGCAGGTTCTCAAAAGGATTACCTATGACCACTCGGTGAGGGTGGTCGTGCTTACTGGCGCGGGTCGAGGATTCTGCTCT
GGTGCAGATCAAAAGTTCACGGCACCTGTGCCACAGGTCGAGGGGTTGACACAGCCGGTTCGCGCGTTGCGTGCCATGGA
GCTTCTTGAAGAAGTCATCCTGGCTCTGCGGCGATTGCACCAACCTGTGATCGCCGCGATCAATGGTCCGGCCATCGGCG
GTGGGTTGTGTCTGGCGTTGGCCGCAGACGTCCGGGTGGCTTCAACTAGGGCCTACTTTCGAGCTGCTGGCATCAACAAC
GGCCTTAGTGCCAGTGAGCTGGGGCTGAGCTATTTGCTGCCTCGGGCCGTCGGATCGTCGCGGGCTTTTGAAATCATGCT
TAGCGGTCGCGATGTCGGGGCGGAGGAAGCCGAGCAGATCGGGCTGGTGTCATATCGGGTGTCGGACGATCGGCTTCTGG
ACACTTGCTACTCCATCGCCGCGCGGATGGCGACGTTCTCGCGGTCTGGAACTGAGTTGACGAAGCGGGCTTTGTGGGGT
GGCTTGGATGCCGCCAGTCTGGATAAGCACATGCAAAGCGAGAGCCTGGCACAGCTCTTTATAGCATTGCATACCAGCAA
TTTTGAAGAAGCGGCTGCCCCATGCACCGAGAAGCGGCCAACGGTGTTAGTCGATGCCAGAGGCTGCGCCACAAGCCCGG
GGTAA

Upstream 100 bases:

>100_bases
TAACTTTACTGTAGTCCTTTTGCACCATATACGTGTTGTTTTTCGCATTTATACGCGTTTCAGCCGATGTTTCCGGTTAT
AATGGCTGCAAGCTGTTCGT

Downstream 100 bases:

>100_bases
GGCTTTCGAATAACCATACTTCACGCTGATTGTTTGCTAACAAAGATTTTTTGCTAACAAGCATGGTCATTCCATGTCTT
CCAAGTCACGCGATGAATTT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSQTDASCTIAELPYRSVTDLVVLDFPRPEVALITLNRPGRMNSMALDLMKSLKQVLKRITYDHSVRVVVLTGAGRGFCS
GADQKFTAPVPQVEGLTQPVRALRAMELLEEVILALRRLHQPVIAAINGPAIGGGLCLALAADVRVASTRAYFRAAGINN
GLSASELGLSYLLPRAVGSSRAFEIMLSGRDVGAEEAEQIGLVSYRVSDDRLLDTCYSIAARMATFSRSGTELTKRALWG
GLDAASLDKHMQSESLAQLFIALHTSNFEEAAAPCTEKRPTVLVDARGCATSPG

Sequences:

>Translated_294_residues
MSQTDASCTIAELPYRSVTDLVVLDFPRPEVALITLNRPGRMNSMALDLMKSLKQVLKRITYDHSVRVVVLTGAGRGFCS
GADQKFTAPVPQVEGLTQPVRALRAMELLEEVILALRRLHQPVIAAINGPAIGGGLCLALAADVRVASTRAYFRAAGINN
GLSASELGLSYLLPRAVGSSRAFEIMLSGRDVGAEEAEQIGLVSYRVSDDRLLDTCYSIAARMATFSRSGTELTKRALWG
GLDAASLDKHMQSESLAQLFIALHTSNFEEAAAPCTEKRPTVLVDARGCATSPG
>Mature_293_residues
SQTDASCTIAELPYRSVTDLVVLDFPRPEVALITLNRPGRMNSMALDLMKSLKQVLKRITYDHSVRVVVLTGAGRGFCSG
ADQKFTAPVPQVEGLTQPVRALRAMELLEEVILALRRLHQPVIAAINGPAIGGGLCLALAADVRVASTRAYFRAAGINNG
LSASELGLSYLLPRAVGSSRAFEIMLSGRDVGAEEAEQIGLVSYRVSDDRLLDTCYSIAARMATFSRSGTELTKRALWGG
LDAASLDKHMQSESLAQLFIALHTSNFEEAAAPCTEKRPTVLVDARGCATSPG

Specific function: Could possibly oxidize fatty acids using specific components

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=29.296875, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI68989263, Length=181, Percent_Identity=34.2541436464088, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI70995211, Length=245, Percent_Identity=31.0204081632653, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI31542718, Length=225, Percent_Identity=29.7777777777778, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI37594469, Length=184, Percent_Identity=27.1739130434783, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594471, Length=184, Percent_Identity=27.1739130434783, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45643119, Length=199, Percent_Identity=29.1457286432161, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI260275230, Length=199, Percent_Identity=29.1457286432161, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI260274832, Length=199, Percent_Identity=29.1457286432161, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI20127408, Length=185, Percent_Identity=30.2702702702703, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI116235487, Length=164, Percent_Identity=29.2682926829268, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI221136756, Length=164, Percent_Identity=29.2682926829268, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI221136753, Length=164, Percent_Identity=29.2682926829268, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=164, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=32.6771653543307, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787659, Length=210, Percent_Identity=34.7619047619048, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788597, Length=201, Percent_Identity=31.3432835820896, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI221142681, Length=196, Percent_Identity=33.1632653061224, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790281, Length=209, Percent_Identity=29.1866028708134, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788682, Length=275, Percent_Identity=28.3636363636364, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=274, Percent_Identity=28.8321167883212, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=35.0393700787402, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=260, Percent_Identity=31.1538461538462, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=185, Percent_Identity=35.1351351351351, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=211, Percent_Identity=32.2274881516588, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=180, Percent_Identity=33.8888888888889, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=185, Percent_Identity=28.1081081081081, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=213, Percent_Identity=28.6384976525822, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=246, Percent_Identity=22.7642276422764, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=31.3725490196078, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=31.3725490196078, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653139, Length=220, Percent_Identity=33.6363636363636, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19920382, Length=240, Percent_Identity=29.5833333333333, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24654903, Length=170, Percent_Identity=31.7647058823529, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21357171, Length=206, Percent_Identity=31.0679611650485, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922422, Length=247, Percent_Identity=25.5060728744939, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=30.2439024390244, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ECH12_MYCLE (P53526)

Other databases:

- EMBL:   U00010
- EMBL:   AL049913
- EMBL:   AL583921
- PIR:   S72706
- RefSeq:   NP_301896.1
- ProteinModelPortal:   P53526
- SMR:   P53526
- EnsemblBacteria:   EBMYCT00000028127
- GeneID:   910343
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1241
- NMPDR:   fig|272631.1.peg.768
- Leproma:   ML1241
- GeneTree:   EBGT00050000015506
- HOGENOM:   HBG748731
- OMA:   LEGHMQA
- ProtClustDB:   PRK05864
- BioCyc:   MLEP272631:ML1241-MONOMER
- BRENDA:   4.2.1.17
- InterPro:   IPR001753
- InterPro:   IPR018376

Pfam domain/function: PF00378 ECH

EC number: =4.2.1.17

Molecular weight: Translated: 31492; Mature: 31360

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTDASCTIAELPYRSVTDLVVLDFPRPEVALITLNRPGRMNSMALDLMKSLKQVLKRI
CCCCCCCEEEECCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
TYDHSVRVVVLTGAGRGFCSGADQKFTAPVPQVEGLTQPVRALRAMELLEEVILALRRLH
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
QPVIAAINGPAIGGGLCLALAADVRVASTRAYFRAAGINNGLSASELGLSYLLPRAVGSS
CHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
RAFEIMLSGRDVGAEEAEQIGLVSYRVSDDRLLDTCYSIAARMATFSRSGTELTKRALWG
CEEEEEEECCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
GLDAASLDKHMQSESLAQLFIALHTSNFEEAAAPCTEKRPTVLVDARGCATSPG
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
SQTDASCTIAELPYRSVTDLVVLDFPRPEVALITLNRPGRMNSMALDLMKSLKQVLKRI
CCCCCCEEEECCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
TYDHSVRVVVLTGAGRGFCSGADQKFTAPVPQVEGLTQPVRALRAMELLEEVILALRRLH
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
QPVIAAINGPAIGGGLCLALAADVRVASTRAYFRAAGINNGLSASELGLSYLLPRAVGSS
CHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
RAFEIMLSGRDVGAEEAEQIGLVSYRVSDDRLLDTCYSIAARMATFSRSGTELTKRALWG
CEEEEEEECCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
GLDAASLDKHMQSESLAQLFIALHTSNFEEAAAPCTEKRPTVLVDARGCATSPG
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002