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The map label for this gene is ilvA

Identifier: 221230090

GI number: 221230090

Start: 1428278

End: 1429561

Strand: Direct

Name: ilvA

Synonym: MLBr_01209

Alternate gene names: 221230090

Gene position: 1428278-1429561 (Clockwise)

Preceding gene: 221230089

Following gene: 221230091

Centisome position: 43.7

GC content: 61.45

Gene sequence:

>1284_bases
ATGTCCGCCGAACCTAGTAGAAACCCCAGGACCCCACCGGTGTCCGCGGTTGACATTGACGGTGCGGCGAAGCGGATCGC
GCCGGTGGTCACGCCTACCCCGTTGCAACTTAGCGATCGGTTGTCGGCGATCACTGGTGCAGCGGTGTACCTCAAGCGTG
AAGACCTGCAGACGGTGCGCTCTTACAAACTGCGCGGCGCCTACAACCTGTTGGTGCAGCTGACCGACGAGGAGATCGCC
GCCGGCGTTGTGTGCTCTTCGGCGGGTAACCATGCACAGGGTGTCGCGTATGCATGTCGGTCACTGGGTGTGCACGGCCG
TGTCTATGTACCTGCCAAGACACCCAAACAAAAGTGGGATCGGATCCGCTACCATGGCGGGGCATTCATCGAGCTGATCG
TCGGGAGATCGACCTACGATCTCGCCGCTGCCGCGGCGGTCGACGACATTGAACGCACCGGCGCGACGTTGGTACCTCCT
TATGACGACGTCCGCGTCATCGCTGGTCAGGGCACCATTGCCGTCGAGTTGCTGGAGCAACTCAACACCGAGCCTGACCT
AGTGGTGGTCCCGGTGGGCGGCGGTGGCTGCATCGCCGGCATGACCACTTACTTGGCCGAACGAACGGCGAACACTGCTG
TGCTTGGTGTCGAGCCGGCTGGTGCCGCGGCTATGATGGCCGCGCTGGCCGCGGGTGAGCCGGTGACACTGGACTACGTC
GATCAGTTCGTGGATGGCGCTGCGGTGAACCGGGTGGGAACGTTGCCTTATGCCGCGTTGACTGCCGCAGGTGACATGGT
GTCCATCACCACCGTCGACGAGGGGGCGGTGTGCACTGCGATGCTCGACCTTTACCAGAACGAGGGCATTATAGCTGAGC
CGGCGGGCGCACTGTCAGTAGCTGGGCTGCTGGAAACTGACATTGAACCCGGGTCCACCGTGGTTTGCCTGATCTCGGGC
GGCAACAACGACGTATTGCGCTACGGTGAGGTGTTGGAGCGTTCGCTGATCCACCTAGGTCTCAAACACTATTTCCTGGT
AGACTTCCCCCAGAAGCCTGGTGCCTTGCGCCGTTTTCTTGACGAAGTGCTTGGACCCAACGACGACATCACCTTGTTCG
AGTACGTCAAGCGCAACAACCGAGAGACCGGCGAGGCGCTGGTAGGTATCCAGCTGGGGTCTGCTGTCGACTTAGACGTC
CTGCTGGCCCGGATGCAGGGGACCGAAATGCACGTCGAAACCCTACAACCAGGTTCGCCGGCCTACCGCTACCTGTTGCT
TTAG

Upstream 100 bases:

>100_bases
ACTACGTCAACTACCAGACCAAAACTGAACGTTAGATTCCGGTCTTTGTGCTGACTCCGGTGAGCTGATTGCTTGTCGGA
CGACATGGCACCATGGACCT

Downstream 100 bases:

>100_bases
ACGAGAACGCACGTAGTTGTACACGTTTTACGGCGTATCGCTGTGCAAATACGCGCGCTCACAGCAGGCGAGTGGCGAGT
CCAGGTTTGCGGTCAGGTAT

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSAEPSRNPRTPPVSAVDIDGAAKRIAPVVTPTPLQLSDRLSAITGAAVYLKREDLQTVRSYKLRGAYNLLVQLTDEEIA
AGVVCSSAGNHAQGVAYACRSLGVHGRVYVPAKTPKQKWDRIRYHGGAFIELIVGRSTYDLAAAAAVDDIERTGATLVPP
YDDVRVIAGQGTIAVELLEQLNTEPDLVVVPVGGGGCIAGMTTYLAERTANTAVLGVEPAGAAAMMAALAAGEPVTLDYV
DQFVDGAAVNRVGTLPYAALTAAGDMVSITTVDEGAVCTAMLDLYQNEGIIAEPAGALSVAGLLETDIEPGSTVVCLISG
GNNDVLRYGEVLERSLIHLGLKHYFLVDFPQKPGALRRFLDEVLGPNDDITLFEYVKRNNRETGEALVGIQLGSAVDLDV
LLARMQGTEMHVETLQPGSPAYRYLLL

Sequences:

>Translated_427_residues
MSAEPSRNPRTPPVSAVDIDGAAKRIAPVVTPTPLQLSDRLSAITGAAVYLKREDLQTVRSYKLRGAYNLLVQLTDEEIA
AGVVCSSAGNHAQGVAYACRSLGVHGRVYVPAKTPKQKWDRIRYHGGAFIELIVGRSTYDLAAAAAVDDIERTGATLVPP
YDDVRVIAGQGTIAVELLEQLNTEPDLVVVPVGGGGCIAGMTTYLAERTANTAVLGVEPAGAAAMMAALAAGEPVTLDYV
DQFVDGAAVNRVGTLPYAALTAAGDMVSITTVDEGAVCTAMLDLYQNEGIIAEPAGALSVAGLLETDIEPGSTVVCLISG
GNNDVLRYGEVLERSLIHLGLKHYFLVDFPQKPGALRRFLDEVLGPNDDITLFEYVKRNNRETGEALVGIQLGSAVDLDV
LLARMQGTEMHVETLQPGSPAYRYLLL
>Mature_426_residues
SAEPSRNPRTPPVSAVDIDGAAKRIAPVVTPTPLQLSDRLSAITGAAVYLKREDLQTVRSYKLRGAYNLLVQLTDEEIAA
GVVCSSAGNHAQGVAYACRSLGVHGRVYVPAKTPKQKWDRIRYHGGAFIELIVGRSTYDLAAAAAVDDIERTGATLVPPY
DDVRVIAGQGTIAVELLEQLNTEPDLVVVPVGGGGCIAGMTTYLAERTANTAVLGVEPAGAAAMMAALAAGEPVTLDYVD
QFVDGAAVNRVGTLPYAALTAAGDMVSITTVDEGAVCTAMLDLYQNEGIIAEPAGALSVAGLLETDIEPGSTVVCLISGG
NNDVLRYGEVLERSLIHLGLKHYFLVDFPQKPGALRRFLDEVLGPNDDITLFEYVKRNNRETGEALVGIQLGSAVDLDVL
LARMQGTEMHVETLQPGSPAYRYLLL

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family

Homologues:

Organism=Homo sapiens, GI11345492, Length=319, Percent_Identity=27.8996865203762, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI33469958, Length=323, Percent_Identity=30.030959752322, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI19923959, Length=324, Percent_Identity=29.320987654321, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790207, Length=371, Percent_Identity=37.7358490566038, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789505, Length=322, Percent_Identity=35.7142857142857, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537387, Length=382, Percent_Identity=32.7225130890052, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71991565, Length=336, Percent_Identity=31.8452380952381, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17508781, Length=318, Percent_Identity=27.6729559748428, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6320930, Length=369, Percent_Identity=34.6883468834688, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6322631, Length=316, Percent_Identity=32.5949367088608, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321412, Length=366, Percent_Identity=29.7814207650273, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21355833, Length=356, Percent_Identity=34.8314606741573, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24645328, Length=249, Percent_Identity=33.7349397590361, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): THD1_MYCLE (Q9X7F1)

Other databases:

- EMBL:   AL049478
- EMBL:   AL583921
- PIR:   C87060
- RefSeq:   NP_301876.1
- ProteinModelPortal:   Q9X7F1
- SMR:   Q9X7F1
- EnsemblBacteria:   EBMYCT00000028346
- GeneID:   910312
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1209
- NMPDR:   fig|272631.1.peg.748
- Leproma:   ML1209
- GeneTree:   EBGT00050000015763
- HOGENOM:   HBG714501
- OMA:   VDPFVDG
- ProtClustDB:   PRK08639
- BioCyc:   MLEP272631:ML1209-MONOMER
- BRENDA:   4.3.1.19
- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820
- TIGRFAMs:   TIGR02079

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.3.1.19

Molecular weight: Translated: 45172; Mature: 45041

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEPSRNPRTPPVSAVDIDGAAKRIAPVVTPTPLQLSDRLSAITGAAVYLKREDLQTVR
CCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHCCEEEEEEHHHHHHHH
SYKLRGAYNLLVQLTDEEIAAGVVCSSAGNHAQGVAYACRSLGVHGRVYVPAKTPKQKWD
HHHHCCEEEEEEEECCHHHHHCEEECCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHH
RIRYHGGAFIELIVGRSTYDLAAAAAVDDIERTGATLVPPYDDVRVIAGQGTIAVELLEQ
HHEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEECCCCCCEEEEECCCHHHHHHHHH
LNTEPDLVVVPVGGGGCIAGMTTYLAERTANTAVLGVEPAGAAAMMAALAAGEPVTLDYV
HCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEHHHH
DQFVDGAAVNRVGTLPYAALTAAGDMVSITTVDEGAVCTAMLDLYQNEGIIAEPAGALSV
HHHHCCHHHHHCCCCCHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCEEECCCCHHHH
AGLLETDIEPGSTVVCLISGGNNDVLRYGEVLERSLIHLGLKHYFLVDFPQKPGALRRFL
HHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
DEVLGPNDDITLFEYVKRNNRETGEALVGIQLGSAVDLDVLLARMQGTEMHVETLQPGSP
HHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCC
AYRYLLL
CEEEEEC
>Mature Secondary Structure 
SAEPSRNPRTPPVSAVDIDGAAKRIAPVVTPTPLQLSDRLSAITGAAVYLKREDLQTVR
CCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHCCEEEEEEHHHHHHHH
SYKLRGAYNLLVQLTDEEIAAGVVCSSAGNHAQGVAYACRSLGVHGRVYVPAKTPKQKWD
HHHHCCEEEEEEEECCHHHHHCEEECCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHH
RIRYHGGAFIELIVGRSTYDLAAAAAVDDIERTGATLVPPYDDVRVIAGQGTIAVELLEQ
HHEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEECCCCCCEEEEECCCHHHHHHHHH
LNTEPDLVVVPVGGGGCIAGMTTYLAERTANTAVLGVEPAGAAAMMAALAAGEPVTLDYV
HCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEHHHH
DQFVDGAAVNRVGTLPYAALTAAGDMVSITTVDEGAVCTAMLDLYQNEGIIAEPAGALSV
HHHHCCHHHHHCCCCCHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCEEECCCCHHHH
AGLLETDIEPGSTVVCLISGGNNDVLRYGEVLERSLIHLGLKHYFLVDFPQKPGALRRFL
HHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
DEVLGPNDDITLFEYVKRNNRETGEALVGIQLGSAVDLDVLLARMQGTEMHVETLQPGSP
HHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCC
AYRYLLL
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002