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The map label for this gene is miaB

Identifier: 221229948

GI number: 221229948

Start: 1155771

End: 1157324

Strand: Direct

Name: miaB

Synonym: MLBr_00989

Alternate gene names: 221229948

Gene position: 1155771-1157324 (Clockwise)

Preceding gene: 221229947

Following gene: 221229949

Centisome position: 35.37

GC content: 59.07

Gene sequence:

>1554_bases
GTGGTGACGCGTGACGCAGGCATGGCGGATGATGCTGCGACTGGATCGACACGCACTTATCAGGTCCGCACCTACGGCTG
TCAGATGAACGTTCATGATTCTGAGCGGTTGGCGGGTCTGCTTGAGGCCGCCGGTTACCAGCGGGCGGCTGACGAAGCAG
ATGTTGGAGACGCCGATGTGGTGGTTTTCAACACCTGCGCTGTTCGTGAGAACGCTGATAACAGGCTCTACGGTAACCTC
AGCCACCTGGCGCCACGCAAGCGGAACAATCCCGACATGCAAATTGCGGTAGGTGGCTGCCTGGCTCAGAAAGACAAACA
CACGGTGCTGAGCAAGGCGCCGTGGGTCGATATCGTTTTCGGCACTCACAACCTGGGATCGCTGCCTACGCTCCTTGACC
GGGCTCGCCACAATAAGGTCGCTCAGGTGGAAATTGTTGAAGCGCTCCAACATTTTCCGTCATCATTGCCGAGTGCCCGC
GAATCCGATTATGCTGCTTGGGTTTCTATCTCAGTTGGATGCAACAACAGCTGTACCTTCTGTATTGTTCCGTCGCTACG
AGGTAAGGAGGTCGATCGCAGTCCTGCTGACATCTTGGCTGAGGTGGAAGCACTGGTTGCTGACGGCGTGCTAGAAGTAA
CTTTGTTGGGGCAGAACGTCAATGCTTACGGGGTCTCTTTCGCTGACCCGGCGCTTGCCCGCAATCGTGGTTCCTTCGCC
GAATTGCTGCGGTCCTGCGGGAGTATCGATGGACTCGAACGCGTACGGTTCACCTCTCCGCATCCTGCCGAATTCACTTA
CGATGTCATCGAGGCAATGGCGCAGACGCCCAACGTGTGTCCCTCCTTGCACATGCCGCTGCAGTCCGGGTCTGACCGGG
TGCTACGTGCGATGCGGCGGTCTTACCGCGTCGAGCGTTATCTTGGCATTATCGGCCAGGTTCGGGCGGCCATGCCACAC
GCCGCCATCACCACAGATCTCATTGTGGGATTTCCCGGTGAGACCGAAGAGGACTTTGCGGCCACGCTTGATGTGGTGCG
TCAGGTCCGGTTTACGGCGGCGTTCACCTTCCAGTACTCTAAGCGCCCCGGAACCCCGGCCGCTGAACTTGGTGGGCAGC
TGCCGAAGGCCGTTGTGCAGGAACGCTACGAACGGCTAGTTGAGTTGCAGGAGCAGATTTGTATGGAGGAAAATCGTGTG
CTGATTGGGCGGATTGTCGAACTGCTAGTCACCACTGGCGAAGGACGCAAAGATGCCCGCACCGCGCGCAGGACTGGGCG
CGCACGCGATGGCCGGTTGGTGCATTTCGCCTTGGACACGCCCTGGGAGGCGCAGGTGCGGCCCGGTGATATCATCACCG
TGATGGTCACTGAAGCAGCGCCGCACCATCTGATAGCCGATGCGGGTATATTAACTCACCGCCGCACCCGTGCTGGGGAT
GCACACGCCGCCAGGCAGTGCCCACGCAGCATTGGGCTTGGCCAAATTCCTTTGGGTATGCCGAGTGTCGGTCAGCATTC
GGCTTCGATTGGGTCTGCTGGATGTGCCCGATGA

Upstream 100 bases:

>100_bases
GTCGCCGAGCTTGCAGCCGAACGAGAGCGGCGCCGGGTCTAATTAAGCTGCGGGTTAACTCGGTGGGACCCGGTCGCCGT
ACTATTGCCCCGTGACTTCG

Downstream 100 bases:

>100_bases
ACGAAGAACCCAATATCGTGGACTTCCCGGATTCCAATCCCCTCCAGGCTGCACTTGAGGCAGAGGAACTTCGGGTAGTC
CGTGAGATTGATTCTGGAGC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MVTRDAGMADDAATGSTRTYQVRTYGCQMNVHDSERLAGLLEAAGYQRAADEADVGDADVVVFNTCAVRENADNRLYGNL
SHLAPRKRNNPDMQIAVGGCLAQKDKHTVLSKAPWVDIVFGTHNLGSLPTLLDRARHNKVAQVEIVEALQHFPSSLPSAR
ESDYAAWVSISVGCNNSCTFCIVPSLRGKEVDRSPADILAEVEALVADGVLEVTLLGQNVNAYGVSFADPALARNRGSFA
ELLRSCGSIDGLERVRFTSPHPAEFTYDVIEAMAQTPNVCPSLHMPLQSGSDRVLRAMRRSYRVERYLGIIGQVRAAMPH
AAITTDLIVGFPGETEEDFAATLDVVRQVRFTAAFTFQYSKRPGTPAAELGGQLPKAVVQERYERLVELQEQICMEENRV
LIGRIVELLVTTGEGRKDARTARRTGRARDGRLVHFALDTPWEAQVRPGDIITVMVTEAAPHHLIADAGILTHRRTRAGD
AHAARQCPRSIGLGQIPLGMPSVGQHSASIGSAGCAR

Sequences:

>Translated_517_residues
MVTRDAGMADDAATGSTRTYQVRTYGCQMNVHDSERLAGLLEAAGYQRAADEADVGDADVVVFNTCAVRENADNRLYGNL
SHLAPRKRNNPDMQIAVGGCLAQKDKHTVLSKAPWVDIVFGTHNLGSLPTLLDRARHNKVAQVEIVEALQHFPSSLPSAR
ESDYAAWVSISVGCNNSCTFCIVPSLRGKEVDRSPADILAEVEALVADGVLEVTLLGQNVNAYGVSFADPALARNRGSFA
ELLRSCGSIDGLERVRFTSPHPAEFTYDVIEAMAQTPNVCPSLHMPLQSGSDRVLRAMRRSYRVERYLGIIGQVRAAMPH
AAITTDLIVGFPGETEEDFAATLDVVRQVRFTAAFTFQYSKRPGTPAAELGGQLPKAVVQERYERLVELQEQICMEENRV
LIGRIVELLVTTGEGRKDARTARRTGRARDGRLVHFALDTPWEAQVRPGDIITVMVTEAAPHHLIADAGILTHRRTRAGD
AHAARQCPRSIGLGQIPLGMPSVGQHSASIGSAGCAR
>Mature_517_residues
MVTRDAGMADDAATGSTRTYQVRTYGCQMNVHDSERLAGLLEAAGYQRAADEADVGDADVVVFNTCAVRENADNRLYGNL
SHLAPRKRNNPDMQIAVGGCLAQKDKHTVLSKAPWVDIVFGTHNLGSLPTLLDRARHNKVAQVEIVEALQHFPSSLPSAR
ESDYAAWVSISVGCNNSCTFCIVPSLRGKEVDRSPADILAEVEALVADGVLEVTLLGQNVNAYGVSFADPALARNRGSFA
ELLRSCGSIDGLERVRFTSPHPAEFTYDVIEAMAQTPNVCPSLHMPLQSGSDRVLRAMRRSYRVERYLGIIGQVRAAMPH
AAITTDLIVGFPGETEEDFAATLDVVRQVRFTAAFTFQYSKRPGTPAAELGGQLPKAVVQERYERLVELQEQICMEENRV
LIGRIVELLVTTGEGRKDARTARRTGRARDGRLVHFALDTPWEAQVRPGDIITVMVTEAAPHHLIADAGILTHRRTRAGD
AHAARQCPRSIGLGQIPLGMPSVGQHSASIGSAGCAR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=486, Percent_Identity=34.156378600823, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI28872782, Length=486, Percent_Identity=34.156378600823, Blast_Score=262, Evalue=5e-70,
Organism=Homo sapiens, GI93277076, Length=398, Percent_Identity=27.6381909547739, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1786882, Length=453, Percent_Identity=40.8388520971302, Blast_Score=328, Evalue=6e-91,
Organism=Escherichia coli, GI1787057, Length=449, Percent_Identity=31.6258351893096, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17553146, Length=477, Percent_Identity=32.0754716981132, Blast_Score=224, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI71996771, Length=223, Percent_Identity=35.8744394618834, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21356207, Length=431, Percent_Identity=36.4269141531323, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI19922432, Length=411, Percent_Identity=29.9270072992701, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_MYCLE (Q49842)

Other databases:

- EMBL:   U00019
- EMBL:   AL583920
- PIR:   G87032
- PIR:   S72937
- RefSeq:   NP_301734.1
- ProteinModelPortal:   Q49842
- SMR:   Q49842
- EnsemblBacteria:   EBMYCT00000029271
- GeneID:   910281
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0989
- NMPDR:   fig|272631.1.peg.606
- Leproma:   ML0989
- GeneTree:   EBGT00050000015784
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14326
- BioCyc:   MLEP272631:ML0989-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 56123; Mature: 56123

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTRDAGMADDAATGSTRTYQVRTYGCQMNVHDSERLAGLLEAAGYQRAADEADVGDADV
CCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCCCCCCE
VVFNTCAVRENADNRLYGNLSHLAPRKRNNPDMQIAVGGCLAQKDKHTVLSKAPWVDIVF
EEEEEEEECCCCCCCEECCHHHHCCCCCCCCCEEEEECCHHCCCCCHHHHCCCCEEEEEE
GTHNLGSLPTLLDRARHNKVAQVEIVEALQHFPSSLPSARESDYAAWVSISVGCNNSCTF
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCE
CIVPSLRGKEVDRSPADILAEVEALVADGVLEVTLLGQNVNAYGVSFADPALARNRGSFA
EEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHCCCCHHH
ELLRSCGSIDGLERVRFTSPHPAEFTYDVIEAMAQTPNVCPSLHMPLQSGSDRVLRAMRR
HHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
SYRVERYLGIIGQVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRQVRFTAAFTFQYS
HHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEC
KRPGTPAAELGGQLPKAVVQERYERLVELQEQICMEENRVLIGRIVELLVTTGEGRKDAR
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHH
TARRTGRARDGRLVHFALDTPWEAQVRPGDIITVMVTEAAPHHLIADAGILTHRRTRAGD
HHHHCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHCCCHHHCCCCCCCC
AHAARQCPRSIGLGQIPLGMPSVGQHSASIGSAGCAR
HHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVTRDAGMADDAATGSTRTYQVRTYGCQMNVHDSERLAGLLEAAGYQRAADEADVGDADV
CCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCCCCCCE
VVFNTCAVRENADNRLYGNLSHLAPRKRNNPDMQIAVGGCLAQKDKHTVLSKAPWVDIVF
EEEEEEEECCCCCCCEECCHHHHCCCCCCCCCEEEEECCHHCCCCCHHHHCCCCEEEEEE
GTHNLGSLPTLLDRARHNKVAQVEIVEALQHFPSSLPSARESDYAAWVSISVGCNNSCTF
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCE
CIVPSLRGKEVDRSPADILAEVEALVADGVLEVTLLGQNVNAYGVSFADPALARNRGSFA
EEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHCCCCHHH
ELLRSCGSIDGLERVRFTSPHPAEFTYDVIEAMAQTPNVCPSLHMPLQSGSDRVLRAMRR
HHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
SYRVERYLGIIGQVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRQVRFTAAFTFQYS
HHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEC
KRPGTPAAELGGQLPKAVVQERYERLVELQEQICMEENRVLIGRIVELLVTTGEGRKDAR
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHH
TARRTGRARDGRLVHFALDTPWEAQVRPGDIITVMVTEAAPHHLIADAGILTHRRTRAGD
HHHHCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHCCCHHHCCCCCCCC
AHAARQCPRSIGLGQIPLGMPSVGQHSASIGSAGCAR
HHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002