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The map label for this gene is recA

Identifier: 221229946

GI number: 221229946

Start: 1153096

End: 1155231

Strand: Direct

Name: recA

Synonym: MLBr_00987

Alternate gene names: 221229946

Gene position: 1153096-1155231 (Clockwise)

Preceding gene: 221229945

Following gene: 221229947

Centisome position: 35.28

GC content: 56.04

Gene sequence:

>2136_bases
ATGGCACAAGTCCCCGACCGTGAGAAAGCTCTCGAGCTGGCGATGGCCCAGATCGAGAAAAACTATGGCAAAGGTTCGGT
GATGCGTCTCGGTGACGAGATGTGTCAGCCAATCTCGGTCATTCCGACCGGCTCCATCGCACTGGACGTGGCTCTGGGTA
TTGGCGGTCTGCCGCGTGGCCGGATTGTGGAAATCTACGGTCCGGAGTCCTCGGGCAAAACTACTGTTGCACTACACGCT
GTGGCCAATGCCCAGGCGGTCGGCGGTGTCGCAGCGTTCATCGACGCCGAGCACGCGCTGGAACCGGAGTATGCTAAGAA
GCTCGGGGTTGATACCGATTCCTTGCTGGTAAGTCAACCTGATACCGGTGAACAAGCCCTCGAGATCGCCGACATGCTGA
TCCGTTCTGGCGCGCTCGACATTGTGGTCATCGACTCGGTTGCTGCGCTGGTGCCGCGCGCGGAACTAGAAGGTGAGATG
GGGGACAGCTATGTCGGATTGCAAGCCCGGCTGATGAGTCAGGCACTGAGGAAAATGACTGGTGCGCTGAGTAATTCGGG
AACCACGGCGATCTTCATCAACCAGCTACGTGAGAAGATTGGGGTGATGTTCGGGTGCATGAATTACTCGACCCGCGTGA
CTCTTGCGGACGGTTCCACTGAAAAGATCGGCAAGATCGTCAACAACAAAATGGATGTCAGGGTGTTGTCCTACGACCCG
GTGACGGACCGGATTGTGCCACGCAAGGTAGTGAACTGGTTCAATAATGGTCCAGCTGAACAGTTCCTCCAATTTACTGT
CGAAAAGTCCGGTAGTAACGGCAAGTCACAGTTTGCGGCGACGCCCAACCACCTCATTCGCACACCTGGCGGCTGGACAG
AAGCCGGTAACCTTATTGCCGGTGATCGGGTGCTTGCCGTCGAACCACATATGCTGAGCGATCAGCAGTTCCAAGTTGTG
TTGGGCTCACTGATGGGTGACGGGAACCTCTCGCCCAATCTGTGCGATCGTAACGGTGTCCGGTTCCGGTTGTTGGGATA
CGGCTGCAAGCAGGTGGAATATCTGCAGTGGAAGAAGGCGCTAATGGGCAACATTCGGCATACGGTGCGGGAGAATTCCA
TGGGCGCGAGTTTTATCGACTTCACCCCATTGCCAGAGCTCGTCGAGCTACAGCGCGCGGTCTACCTCGGAGACGGTAAG
AAGTTCTTATCTGAAGAGTATCTCAAGGCGCTGACGCCGTTGGTTCTGGCTATTTGGTATATGGACGATGGTTCATTCAC
AGTGGGTTCCAAGAGGGTGCAGGAACGTACCGCCGGTGGCAGCGGGCGCATTGAGATCTGTGTAGATGCCATGACCGAAG
GCACACGTGTACGTTTGCGCGACTATCTGTGCGACACACACGGCTTGGATGTGCGTTTGCGAGAAGTTGGTTCGGCCGGT
AAGGCGGTGCTGGTGTTTTCCACTGCGGCCACTGCGAAGTTCCAGTCATTGATCGCTCCATATGTGGCGCCGTCCATGGA
GTACAAGTTGTTGCCGCAGTTCCGTGGTCGGGGGAGCGTGACACCGCAGTTCGTCGAACCCACGCAGCAGTTGGTGCCGG
CCCGCGTTCTCGATGTTCATGTCAAACTGTCTACCCGATCAATGAACCGGTTCGATATCGAGGTTGAGGGCAATCACAAC
TACTTCGTTGACGGAGTGATGGTACACAATTCTCCCGAAACCACGACGGGTGGAAAGGCGTTGAAATTCTATGCGTCGGT
GCGCATGGACGTGCGTCGGATTGAGACACTTAAGGACGGTGTCGATGCGGTCGGCAACCGGACCCGGGTCAAAATCGTCA
AAAATAAGGTGTCGCCACCTTTCAAGCAGGCCGAGTTCGACATCCTGTACGGCAAGGGCATTAGCAGGGAGGGTTCACTG
ATCGATATGGGTGTGGAACAAGGCTTTGTCCGCAAGTCTGGATCCTGGTTCACTTATGAGGGCGAACAGCTTGGCCAGGG
CAAGGAGAATGCGCGCAATTTCTTGCTGGAGAACGCCGATGTGGCCAATGAGATCGAGAAGAAGATCAAGGAGAAGCTTG
GCATTGGCGCGGTCGTGACCGATGACGACATCCTGCCCACCCCCGTCGATTTCTGA

Upstream 100 bases:

>100_bases
CGGATATTGTGGTGGTTATTCAGCGATGTCGGCTTGTCGGTGGCTCTCTAGTGTCACGTCCAACCGACCGGTACCGGTTA
CCGACTACAGGAGAAGTAAC

Downstream 100 bases:

>100_bases
TCGCGAAGAGCAGGCACGGATATTGTGCCTGCGCCTGCTCACCGCGAGATCGCGAACGCGAGCCCAGCTATTCGGCCAGC
TGGCGAAACGCGGATACGCC

Product: RecA protein

Products: NA

Alternate protein names: Recombinase A

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MAQVPDREKALELAMAQIEKNYGKGSVMRLGDEMCQPISVIPTGSIALDVALGIGGLPRGRIVEIYGPESSGKTTVALHA
VANAQAVGGVAAFIDAEHALEPEYAKKLGVDTDSLLVSQPDTGEQALEIADMLIRSGALDIVVIDSVAALVPRAELEGEM
GDSYVGLQARLMSQALRKMTGALSNSGTTAIFINQLREKIGVMFGCMNYSTRVTLADGSTEKIGKIVNNKMDVRVLSYDP
VTDRIVPRKVVNWFNNGPAEQFLQFTVEKSGSNGKSQFAATPNHLIRTPGGWTEAGNLIAGDRVLAVEPHMLSDQQFQVV
LGSLMGDGNLSPNLCDRNGVRFRLLGYGCKQVEYLQWKKALMGNIRHTVRENSMGASFIDFTPLPELVELQRAVYLGDGK
KFLSEEYLKALTPLVLAIWYMDDGSFTVGSKRVQERTAGGSGRIEICVDAMTEGTRVRLRDYLCDTHGLDVRLREVGSAG
KAVLVFSTAATAKFQSLIAPYVAPSMEYKLLPQFRGRGSVTPQFVEPTQQLVPARVLDVHVKLSTRSMNRFDIEVEGNHN
YFVDGVMVHNSPETTTGGKALKFYASVRMDVRRIETLKDGVDAVGNRTRVKIVKNKVSPPFKQAEFDILYGKGISREGSL
IDMGVEQGFVRKSGSWFTYEGEQLGQGKENARNFLLENADVANEIEKKIKEKLGIGAVVTDDDILPTPVDF

Sequences:

>Translated_711_residues
MAQVPDREKALELAMAQIEKNYGKGSVMRLGDEMCQPISVIPTGSIALDVALGIGGLPRGRIVEIYGPESSGKTTVALHA
VANAQAVGGVAAFIDAEHALEPEYAKKLGVDTDSLLVSQPDTGEQALEIADMLIRSGALDIVVIDSVAALVPRAELEGEM
GDSYVGLQARLMSQALRKMTGALSNSGTTAIFINQLREKIGVMFGCMNYSTRVTLADGSTEKIGKIVNNKMDVRVLSYDP
VTDRIVPRKVVNWFNNGPAEQFLQFTVEKSGSNGKSQFAATPNHLIRTPGGWTEAGNLIAGDRVLAVEPHMLSDQQFQVV
LGSLMGDGNLSPNLCDRNGVRFRLLGYGCKQVEYLQWKKALMGNIRHTVRENSMGASFIDFTPLPELVELQRAVYLGDGK
KFLSEEYLKALTPLVLAIWYMDDGSFTVGSKRVQERTAGGSGRIEICVDAMTEGTRVRLRDYLCDTHGLDVRLREVGSAG
KAVLVFSTAATAKFQSLIAPYVAPSMEYKLLPQFRGRGSVTPQFVEPTQQLVPARVLDVHVKLSTRSMNRFDIEVEGNHN
YFVDGVMVHNSPETTTGGKALKFYASVRMDVRRIETLKDGVDAVGNRTRVKIVKNKVSPPFKQAEFDILYGKGISREGSL
IDMGVEQGFVRKSGSWFTYEGEQLGQGKENARNFLLENADVANEIEKKIKEKLGIGAVVTDDDILPTPVDF
>Mature_710_residues
AQVPDREKALELAMAQIEKNYGKGSVMRLGDEMCQPISVIPTGSIALDVALGIGGLPRGRIVEIYGPESSGKTTVALHAV
ANAQAVGGVAAFIDAEHALEPEYAKKLGVDTDSLLVSQPDTGEQALEIADMLIRSGALDIVVIDSVAALVPRAELEGEMG
DSYVGLQARLMSQALRKMTGALSNSGTTAIFINQLREKIGVMFGCMNYSTRVTLADGSTEKIGKIVNNKMDVRVLSYDPV
TDRIVPRKVVNWFNNGPAEQFLQFTVEKSGSNGKSQFAATPNHLIRTPGGWTEAGNLIAGDRVLAVEPHMLSDQQFQVVL
GSLMGDGNLSPNLCDRNGVRFRLLGYGCKQVEYLQWKKALMGNIRHTVRENSMGASFIDFTPLPELVELQRAVYLGDGKK
FLSEEYLKALTPLVLAIWYMDDGSFTVGSKRVQERTAGGSGRIEICVDAMTEGTRVRLRDYLCDTHGLDVRLREVGSAGK
AVLVFSTAATAKFQSLIAPYVAPSMEYKLLPQFRGRGSVTPQFVEPTQQLVPARVLDVHVKLSTRSMNRFDIEVEGNHNY
FVDGVMVHNSPETTTGGKALKFYASVRMDVRRIETLKDGVDAVGNRTRVKIVKNKVSPPFKQAEFDILYGKGISREGSLI
DMGVEQGFVRKSGSWFTYEGEQLGQGKENARNFLLENADVANEIEKKIKEKLGIGAVVTDDDILPTPVDF

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG1372

COG function: function code L; Intein/homing endonuclease

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DOD-type homing endonuclease domain

Homologues:

Organism=Escherichia coli, GI1789051, Length=205, Percent_Identity=65.3658536585366, Blast_Score=276, Evalue=4e-75,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RECA_MYCLE (P35901)

Other databases:

- EMBL:   X73822
- EMBL:   U00019
- EMBL:   AL583920
- PIR:   S37025
- RefSeq:   NP_301732.1
- ProteinModelPortal:   P35901
- EnsemblBacteria:   EBMYCT00000029168
- GeneID:   910009
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0987
- NMPDR:   fig|272631.1.peg.604
- Leproma:   ML0987
- GeneTree:   EBGT00050000016655
- HOGENOM:   HBG129071
- BioCyc:   MLEP272631:ML0987-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00268
- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR004042
- InterPro:   IPR006141
- InterPro:   IPR004860
- InterPro:   IPR020587
- PANTHER:   PTHR22942:SF1
- PRINTS:   PR00142
- SMART:   SM00382
- SMART:   SM00305
- SMART:   SM00306
- TIGRFAMs:   TIGR01443
- TIGRFAMs:   TIGR01445
- TIGRFAMs:   TIGR02012

Pfam domain/function: PF03161 LAGLIDADG_2; PF00154 RecA

EC number: NA

Molecular weight: Translated: 77639; Mature: 77508

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50818 INTEIN_C_TER; PS50819 INTEIN_ENDONUCLEASE; PS50817 INTEIN_N_TER; PS00321 RECA_1; PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVPDREKALELAMAQIEKNYGKGSVMRLGDEMCQPISVIPTGSIALDVALGIGGLPRG
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCHHHCCCEEEECCCCEEEEEEECCCCCCCC
RIVEIYGPESSGKTTVALHAVANAQAVGGVAAFIDAEHALEPEYAKKLGVDTDSLLVSQP
CEEEEECCCCCCCEEEEEEEECCCHHHCCEEEEECHHHCCCCHHHHHHCCCCHHEEEECC
DTGEQALEIADMLIRSGALDIVVIDSVAALVPRAELEGEMGDSYVGLQARLMSQALRKMT
CCCHHHHHHHHHHHHCCCEEEEEECHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHH
GALSNSGTTAIFINQLREKIGVMFGCMNYSTRVTLADGSTEKIGKIVNNKMDVRVLSYDP
HHHCCCCCEEEHHHHHHHHHHHEEEEECCCEEEEEECCCHHHHHHHHCCCCEEEEEECCC
VTDRIVPRKVVNWFNNGPAEQFLQFTVEKSGSNGKSQFAATPNHLIRTPGGWTEAGNLIA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEE
GDRVLAVEPHMLSDQQFQVVLGSLMGDGNLSPNLCDRNGVRFRLLGYGCKQVEYLQWKKA
CCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
LMGNIRHTVRENSMGASFIDFTPLPELVELQRAVYLGDGKKFLSEEYLKALTPLVLAIWY
HHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHEEEEE
MDDGSFTVGSKRVQERTAGGSGRIEICVDAMTEGTRVRLRDYLCDTHGLDVRLREVGSAG
ECCCCEECCHHHHHHHCCCCCCEEEEEEECCCCCCEEEHHHHHCCCCCCCEEEEECCCCC
KAVLVFSTAATAKFQSLIAPYVAPSMEYKLLPQFRGRGSVTPQFVEPTQQLVPARVLDVH
CEEEEEECHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCHHHHCCCEEEEEE
VKLSTRSMNRFDIEVEGNHNYFVDGVMVHNSPETTTGGKALKFYASVRMDVRRIETLKDG
EEEEECCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
VDAVGNRTRVKIVKNKVSPPFKQAEFDILYGKGISREGSLIDMGVEQGFVRKSGSWFTYE
HHHHCCCEEEEEEECCCCCCHHCCCEEEEECCCCCCCCCEEEECCCCCHHCCCCCEEEEC
GEQLGQGKENARNFLLENADVANEIEKKIKEKLGIGAVVTDDDILPTPVDF
CHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
AQVPDREKALELAMAQIEKNYGKGSVMRLGDEMCQPISVIPTGSIALDVALGIGGLPRG
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHCHHHCCCEEEECCCCEEEEEEECCCCCCCC
RIVEIYGPESSGKTTVALHAVANAQAVGGVAAFIDAEHALEPEYAKKLGVDTDSLLVSQP
CEEEEECCCCCCCEEEEEEEECCCHHHCCEEEEECHHHCCCCHHHHHHCCCCHHEEEECC
DTGEQALEIADMLIRSGALDIVVIDSVAALVPRAELEGEMGDSYVGLQARLMSQALRKMT
CCCHHHHHHHHHHHHCCCEEEEEECHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHH
GALSNSGTTAIFINQLREKIGVMFGCMNYSTRVTLADGSTEKIGKIVNNKMDVRVLSYDP
HHHCCCCCEEEHHHHHHHHHHHEEEEECCCEEEEEECCCHHHHHHHHCCCCEEEEEECCC
VTDRIVPRKVVNWFNNGPAEQFLQFTVEKSGSNGKSQFAATPNHLIRTPGGWTEAGNLIA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEE
GDRVLAVEPHMLSDQQFQVVLGSLMGDGNLSPNLCDRNGVRFRLLGYGCKQVEYLQWKKA
CCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
LMGNIRHTVRENSMGASFIDFTPLPELVELQRAVYLGDGKKFLSEEYLKALTPLVLAIWY
HHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHEEEEE
MDDGSFTVGSKRVQERTAGGSGRIEICVDAMTEGTRVRLRDYLCDTHGLDVRLREVGSAG
ECCCCEECCHHHHHHHCCCCCCEEEEEEECCCCCCEEEHHHHHCCCCCCCEEEEECCCCC
KAVLVFSTAATAKFQSLIAPYVAPSMEYKLLPQFRGRGSVTPQFVEPTQQLVPARVLDVH
CEEEEEECHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCHHHHCCCEEEEEE
VKLSTRSMNRFDIEVEGNHNYFVDGVMVHNSPETTTGGKALKFYASVRMDVRRIETLKDG
EEEEECCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
VDAVGNRTRVKIVKNKVSPPFKQAEFDILYGKGISREGSLIDMGVEQGFVRKSGSWFTYE
HHHHCCCEEEEEEECCCCCCHHCCCEEEEECCCCCCCCCEEEECCCCCHHCCCCCEEEEC
GEQLGQGKENARNFLLENADVANEIEKKIKEKLGIGAVVTDDDILPTPVDF
CHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7508863; 11234002