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The map label for this gene is melA [H]

Identifier: 220933162

GI number: 220933162

Start: 2549402

End: 2550700

Strand: Direct

Name: melA [H]

Synonym: Hore_23300

Alternate gene names: 220933162

Gene position: 2549402-2550700 (Clockwise)

Preceding gene: 220933161

Following gene: 220933178

Centisome position: 98.89

GC content: 44.5

Gene sequence:

>1299_bases
ATGCCTAAAATAACATTTCTCGGGGCTGGAAGTACTGTATTTGCTAAAAATGTTCTGGGGGATTGTATGTTAACCCCGGC
CTTAAGAGATTCCCATATCGCCCTGTATGACATTGATCACCAGCGCTTACGGGAATCTGAACAAATGCTTAAAAGTATTA
ATAGAAACTCAAACCAAAACAGGGCAGAAATTGTTGCTTATACAGACCGTAAAGAAGCCCTCCGGGATTCTGACTACGTT
GTTAACGCTATCCAGGTAGGTGGATACAAACCCTGTACTGTTACCGATTTTGAGGTCCCGAAAAAATACGGTTTACGACA
GACCATCGGGGATACCCTGGGTATCGGTGGTATTTTCAGGGCCTTAAGAACTATACCGATCCTCCTGGATTTTGCCAGGG
ATATTGAAGAAGTATGTCCTGACGCCTGGTTTTTAAATTATACCAATCCCATGGCCATTTTAACCGGGGCTATGCTCAAA
GCAACCAATGTTAAGACAGTTGGCCTCTGCCACAGTGTCCAGACCTGTGTTCCTGATCTACTTAAAGACCTGGGAATGAG
TACTGAAAACGTCCAGTGGAAGATAGCCGGTATTAACCACATGGCCTGGCTTTTGGAAATAACCAGAAACGGCCGGGACC
TTTATCCTGAAATTAAAGAAAAGGCCCGGGCCCGAAAAAAACCCCATGATGACATGGTCAGGTATGAAATAATGGAACGC
TTTGGCTACTATGTCACCGAATCCAGTGAACACAATGCTGAATATATGCCCTATTTCATCAAAAGCAACTACCCTGAATT
AATCGAAAAATATAATATTCCTCTCGATGAGTATCCTCGCCGTTGTGAACAACAAATAAAAGACTGGGAAGAGATGAAAG
ACAAACTGGTCCACAATGATAATCTTGAACACAGTCGGACCCATGAATATGCTTCCTATATTATGGAAGCCATGGAAACA
GATAAGCCCTATAAAATTGGGGGGAACGTCCTGAATACAGGGCTTATTACCAACCTCCCTGAAGATGCCTGTGTAGAGGT
TCCCTGCCTGGTGGACAGGAGTGGGGTTACCCCCTGTTATGTGGGAGACCTGCCACCTCAACTGGCTGCCCTGAACCGGA
CAAATATCAATGTTCAGTTGCTGACCATTGAAGCTGCCCTGACCCGGAAAAAAGAATACATTTACCAGGCAGCCATGCTG
GATCCTCATACAGCTGCCGAATTATCTATTGATGAGATTTATGCCCTGGTTGATGATATGATTGAAGCCCATGGTGACTG
GCTCCCTGAATATAAATAA

Upstream 100 bases:

>100_bases
AAGTGTCACATTATTACATATTGAAACAACATTAAACCATTTAAGTTTAATCAAAGTTATTATATACTACAGTTGCAATA
ATAAAAAGGGAGGAATCCAG

Downstream 100 bases:

>100_bases
AAATAACTTATAAGAGCAATTTAACCTTCAGTTGTAATCATTTGATTTTGACCACAATCATCTAATAAGATAAGTGACAG
CCTCGATGGCCCTCTAATTA

Product: glycoside hydrolase family 4

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPKITFLGAGSTVFAKNVLGDCMLTPALRDSHIALYDIDHQRLRESEQMLKSINRNSNQNRAEIVAYTDRKEALRDSDYV
VNAIQVGGYKPCTVTDFEVPKKYGLRQTIGDTLGIGGIFRALRTIPILLDFARDIEEVCPDAWFLNYTNPMAILTGAMLK
ATNVKTVGLCHSVQTCVPDLLKDLGMSTENVQWKIAGINHMAWLLEITRNGRDLYPEIKEKARARKKPHDDMVRYEIMER
FGYYVTESSEHNAEYMPYFIKSNYPELIEKYNIPLDEYPRRCEQQIKDWEEMKDKLVHNDNLEHSRTHEYASYIMEAMET
DKPYKIGGNVLNTGLITNLPEDACVEVPCLVDRSGVTPCYVGDLPPQLAALNRTNINVQLLTIEAALTRKKEYIYQAAML
DPHTAAELSIDEIYALVDDMIEAHGDWLPEYK

Sequences:

>Translated_432_residues
MPKITFLGAGSTVFAKNVLGDCMLTPALRDSHIALYDIDHQRLRESEQMLKSINRNSNQNRAEIVAYTDRKEALRDSDYV
VNAIQVGGYKPCTVTDFEVPKKYGLRQTIGDTLGIGGIFRALRTIPILLDFARDIEEVCPDAWFLNYTNPMAILTGAMLK
ATNVKTVGLCHSVQTCVPDLLKDLGMSTENVQWKIAGINHMAWLLEITRNGRDLYPEIKEKARARKKPHDDMVRYEIMER
FGYYVTESSEHNAEYMPYFIKSNYPELIEKYNIPLDEYPRRCEQQIKDWEEMKDKLVHNDNLEHSRTHEYASYIMEAMET
DKPYKIGGNVLNTGLITNLPEDACVEVPCLVDRSGVTPCYVGDLPPQLAALNRTNINVQLLTIEAALTRKKEYIYQAAML
DPHTAAELSIDEIYALVDDMIEAHGDWLPEYK
>Mature_431_residues
PKITFLGAGSTVFAKNVLGDCMLTPALRDSHIALYDIDHQRLRESEQMLKSINRNSNQNRAEIVAYTDRKEALRDSDYVV
NAIQVGGYKPCTVTDFEVPKKYGLRQTIGDTLGIGGIFRALRTIPILLDFARDIEEVCPDAWFLNYTNPMAILTGAMLKA
TNVKTVGLCHSVQTCVPDLLKDLGMSTENVQWKIAGINHMAWLLEITRNGRDLYPEIKEKARARKKPHDDMVRYEIMERF
GYYVTESSEHNAEYMPYFIKSNYPELIEKYNIPLDEYPRRCEQQIKDWEEMKDKLVHNDNLEHSRTHEYASYIMEAMETD
KPYKIGGNVLNTGLITNLPEDACVEVPCLVDRSGVTPCYVGDLPPQLAALNRTNINVQLLTIEAALTRKKEYIYQAAMLD
PHTAAELSIDEIYALVDDMIEAHGDWLPEYK

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=445, Percent_Identity=52.3595505617978, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1788029, Length=384, Percent_Identity=26.5625, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 49313; Mature: 49182

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKITFLGAGSTVFAKNVLGDCMLTPALRDSHIALYDIDHQRLRESEQMLKSINRNSNQN
CCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCC
RAEIVAYTDRKEALRDSDYVVNAIQVGGYKPCTVTDFEVPKKYGLRQTIGDTLGIGGIFR
CEEEEEECCHHHHHCCCCCEEEEEEECCCCCCEECCCCCCHHCCCHHHHHHHCCHHHHHH
ALRTIPILLDFARDIEEVCPDAWFLNYTNPMAILTGAMLKATNVKTVGLCHSVQTCVPDL
HHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LKDLGMSTENVQWKIAGINHMAWLLEITRNGRDLYPEIKEKARARKKPHDDMVRYEIMER
HHHHCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
FGYYVTESSEHNAEYMPYFIKSNYPELIEKYNIPLDEYPRRCEQQIKDWEEMKDKLVHND
CCCEEECCCCCCCCCCCEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NLEHSRTHEYASYIMEAMETDKPYKIGGNVLNTGLITNLPEDACVEVPCLVDRSGVTPCY
CCCHHHHHHHHHHHHHHHHCCCCEECCCCEECCCCCCCCCCHHHEECCEEECCCCCCCEE
VGDLPPQLAALNRTNINVQLLTIEAALTRKKEYIYQAAMLDPHTAAELSIDEIYALVDDM
ECCCCHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHH
IEAHGDWLPEYK
HHHCCCCCCCCC
>Mature Secondary Structure 
PKITFLGAGSTVFAKNVLGDCMLTPALRDSHIALYDIDHQRLRESEQMLKSINRNSNQN
CCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCC
RAEIVAYTDRKEALRDSDYVVNAIQVGGYKPCTVTDFEVPKKYGLRQTIGDTLGIGGIFR
CEEEEEECCHHHHHCCCCCEEEEEEECCCCCCEECCCCCCHHCCCHHHHHHHCCHHHHHH
ALRTIPILLDFARDIEEVCPDAWFLNYTNPMAILTGAMLKATNVKTVGLCHSVQTCVPDL
HHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LKDLGMSTENVQWKIAGINHMAWLLEITRNGRDLYPEIKEKARARKKPHDDMVRYEIMER
HHHHCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
FGYYVTESSEHNAEYMPYFIKSNYPELIEKYNIPLDEYPRRCEQQIKDWEEMKDKLVHND
CCCEEECCCCCCCCCCCEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NLEHSRTHEYASYIMEAMETDKPYKIGGNVLNTGLITNLPEDACVEVPCLVDRSGVTPCY
CCCHHHHHHHHHHHHHHHHCCCCEECCCCEECCCCCCCCCCHHHEECCEEECCCCCCCEE
VGDLPPQLAALNRTNINVQLLTIEAALTRKKEYIYQAAMLDPHTAAELSIDEIYALVDDM
ECCCCHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHH
IEAHGDWLPEYK
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]