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The map label for this gene is splB [H]

Identifier: 220933059

GI number: 220933059

Start: 2427276

End: 2428298

Strand: Direct

Name: splB [H]

Synonym: Hore_22270

Alternate gene names: 220933059

Gene position: 2427276-2428298 (Clockwise)

Preceding gene: 220933055

Following gene: 220933074

Centisome position: 94.15

GC content: 39.98

Gene sequence:

>1023_bases
TTGCCAGACTTCACACCACAGACGGTATTTGTCCGGGAAAACGCCATGGATTATCCCCTGGGGAAAAAATTATATAATAA
ATTTAAAGAAAGTGGCATTGATACCAGAATAATAGCCTCCCGGGGCCCTTTTCCCTTAAACCCCAATGTGTCTTTCCAGA
AGAAGTTTATCAGGGCCAAAAGGACGGTAGTCGTGTCAGTCCGTTCCCTGGGGCAATTTCAATCCTGTAAACCCTCTGCC
CATTATCAATTACCCCTGGTCACGGGTTGTCCTGCCCATTGCCATTATTGTTATCTCTCTACCAATCTCGGGAAAAACCC
CTATATCAAAATATATGTAAATATTGAAGAAATCCTGAAAAAGGCCGAAAAATATATGGAAGAACGAAAACCGGATATTA
CTATTTTTGAGGGTTCAGCCACTTCAGACCCGGTTCCTGTTGAAGAATGGAGCGGTTCCCTGAAAAGAACTATAAATTAT
TTTGCCGGGACAAAGCTGGGCCGGTTCAGATTTGTAACCAAATTCAGTAACATAGATTCTCTGCTCCACATAAAGCATAA
TAATCATACTGAATTCCGTTTTAGTATAAATAGCAGCTACCCCATAAAAAAATTTGAACCAACCACACCCTCTCCCCGGG
AAAGGATTGAGGCTGCTACCAGGGTTTTTAAGGCCGGTTATCCGATGGGTTTTCTAATAGCCCCTATTTTTATATACGAA
GGATGGCAGGAAGAATACGGTATTCTTTTTAAAGAATTAAAAGATAAACTTAAAACCAGTGGTAAAGGAATATTTTTTGA
GCTCATTACCCACCGCTTTACCAAACGGGCCAAAGAGATTATAGAAGAAATATACCCCAATACCAGACTCCCCATGAATG
AAGAAACCCGCCAGTTTAAATATGGACAATTCGGGTATGGAAAGTATGTTTATCCCCCGGAGACAATGAAAAAAATTGAG
TCATTTATGAGGGAGACAATTAATAAATACTTCCCTGAGGCAGAAATAAAATATTTTGTTTAA

Upstream 100 bases:

>100_bases
GAAACCCTCGCTCAGTTTTATAACCAGAAAATCAATTGCAAAAATAATTTAAGCAGGAAGTTTACTATAACCCTAACCTT
TACTCAAGGAGGTAAATATC

Downstream 100 bases:

>100_bases
ATAAGGGTTATTCATATCAAGGGCTTTCGGAATTGATATTTATAATATATCAGAACTTATTAAAAAATTTACTCAAAAAT
AAAAAACCGGGCGGAACCAT

Product: spore photoproduct lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MPDFTPQTVFVRENAMDYPLGKKLYNKFKESGIDTRIIASRGPFPLNPNVSFQKKFIRAKRTVVVSVRSLGQFQSCKPSA
HYQLPLVTGCPAHCHYCYLSTNLGKNPYIKIYVNIEEILKKAEKYMEERKPDITIFEGSATSDPVPVEEWSGSLKRTINY
FAGTKLGRFRFVTKFSNIDSLLHIKHNNHTEFRFSINSSYPIKKFEPTTPSPRERIEAATRVFKAGYPMGFLIAPIFIYE
GWQEEYGILFKELKDKLKTSGKGIFFELITHRFTKRAKEIIEEIYPNTRLPMNEETRQFKYGQFGYGKYVYPPETMKKIE
SFMRETINKYFPEAEIKYFV

Sequences:

>Translated_340_residues
MPDFTPQTVFVRENAMDYPLGKKLYNKFKESGIDTRIIASRGPFPLNPNVSFQKKFIRAKRTVVVSVRSLGQFQSCKPSA
HYQLPLVTGCPAHCHYCYLSTNLGKNPYIKIYVNIEEILKKAEKYMEERKPDITIFEGSATSDPVPVEEWSGSLKRTINY
FAGTKLGRFRFVTKFSNIDSLLHIKHNNHTEFRFSINSSYPIKKFEPTTPSPRERIEAATRVFKAGYPMGFLIAPIFIYE
GWQEEYGILFKELKDKLKTSGKGIFFELITHRFTKRAKEIIEEIYPNTRLPMNEETRQFKYGQFGYGKYVYPPETMKKIE
SFMRETINKYFPEAEIKYFV
>Mature_339_residues
PDFTPQTVFVRENAMDYPLGKKLYNKFKESGIDTRIIASRGPFPLNPNVSFQKKFIRAKRTVVVSVRSLGQFQSCKPSAH
YQLPLVTGCPAHCHYCYLSTNLGKNPYIKIYVNIEEILKKAEKYMEERKPDITIFEGSATSDPVPVEEWSGSLKRTINYF
AGTKLGRFRFVTKFSNIDSLLHIKHNNHTEFRFSINSSYPIKKFEPTTPSPRERIEAATRVFKAGYPMGFLIAPIFIYEG
WQEEYGILFKELKDKLKTSGKGIFFELITHRFTKRAKEIIEEIYPNTRLPMNEETRQFKYGQFGYGKYVYPPETMKKIES
FMRETINKYFPEAEIKYFV

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 39712; Mature: 39581

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFTPQTVFVRENAMDYPLGKKLYNKFKESGIDTRIIASRGPFPLNPNVSFQKKFIRAK
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
RTVVVSVRSLGQFQSCKPSAHYQLPLVTGCPAHCHYCYLSTNLGKNPYIKIYVNIEEILK
HHHEEEHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEEECHHHHHH
KAEKYMEERKPDITIFEGSATSDPVPVEEWSGSLKRTINYFAGTKLGRFRFVTKFSNIDS
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCEEEEHHHHCCCC
LLHIKHNNHTEFRFSINSSYPIKKFEPTTPSPRERIEAATRVFKAGYPMGFLIAPIFIYE
EEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
GWQEEYGILFKELKDKLKTSGKGIFFELITHRFTKRAKEIIEEIYPNTRLPMNEETRQFK
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCC
YGQFGYGKYVYPPETMKKIESFMRETINKYFPEAEIKYFV
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCEEECC
>Mature Secondary Structure 
PDFTPQTVFVRENAMDYPLGKKLYNKFKESGIDTRIIASRGPFPLNPNVSFQKKFIRAK
CCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
RTVVVSVRSLGQFQSCKPSAHYQLPLVTGCPAHCHYCYLSTNLGKNPYIKIYVNIEEILK
HHHEEEHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEEECHHHHHH
KAEKYMEERKPDITIFEGSATSDPVPVEEWSGSLKRTINYFAGTKLGRFRFVTKFSNIDS
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCEEEEHHHHCCCC
LLHIKHNNHTEFRFSINSSYPIKKFEPTTPSPRERIEAATRVFKAGYPMGFLIAPIFIYE
EEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
GWQEEYGILFKELKDKLKTSGKGIFFELITHRFTKRAKEIIEEIYPNTRLPMNEETRQFK
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCC
YGQFGYGKYVYPPETMKKIESFMRETINKYFPEAEIKYFV
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]