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The map label for this gene is cheB [H]

Identifier: 220932859

GI number: 220932859

Start: 2186716

End: 2187735

Strand: Direct

Name: cheB [H]

Synonym: Hore_20270

Alternate gene names: 220932859

Gene position: 2186716-2187735 (Clockwise)

Preceding gene: 220932847

Following gene: 220932860

Centisome position: 84.82

GC content: 40.98

Gene sequence:

>1020_bases
ATGATAAAAGTAATGGTTGTAGATGACTCTGCCTTTATAAGGCAAATTATGAAAAAAACCATTAATCAGGAAGACGATAT
TGAAGTTATTGCTGTTGCCAGAAATGGTCAGGAATGTCTCGACCTGATTGAAGAAAAACACCCTGACGTAATCACTCTGG
ACATTGATATGCCCGGTAAAGACGGGCTGGAAATACTTAAAGTATTAATGGATAAATACAGTAATATTCCTGTTATTATG
TTAAGTGCTATTGACAACCGGGATACAGTTATGAAAGCCCTGAAACTGGGGGCCTTTGACTTTATTCCAAAACCCTCCGG
GAACATTTCTTTAAATATTAATGATGTTTCAAAACAGTTAATAACTAAAATAAAGGCCGCAGCAGGCAGTAATATATCCT
GTAGTAAGCAGGGTAAGCAAAAGGCCACTGAATTTGATCATAAGCCAGGAGATTTCCCGGTTATTGCCATCGGTAGCTCC
TCGGGAGGGCCAAAGGCCCTGAAAGAAATACTACCTGTTTTTCCGAACAATCTAAAGGCAGCTCTGGTAGTTGTCCAACA
TATGCCACCCGGTTTTACAGCGTCTCTGGCCAAAAGGTTACATCAGGAGTCGAAGATTACCGTAAAAGAAGCCAGCGAAT
ATGATTATCTGCAACCGGGGGTAGCCCTGATAGCCCCGGGGGGCTATCACCTGGAATTTAATCCTGAAGGAAGAGTTGTT
TTAAACCAGAAACCACCGAAATGGGGTGTCCGTCCCTGTGTAGATTATATGCTCAAATCGGTAGCAGACATTTATAAAGA
ACGGGTCATCGGAATAATTTTAACCGGCATGGGCAGAGACGGGGCTGAAGGCATGAAACAGGTAAAAAAATATAATGGTT
ATGGAATTGTTGAGGATAAAAGCACTGCCCTCGTTTATGGAATGCCCGGAGCTACTATTGAAGCCAGAGCTTATGATGAA
ATTCTTCCCCTTCATCAAATACCATTAAAAATAATAGACTTAATAGAAAGGAGAAAATAA

Upstream 100 bases:

>100_bases
AAATCAAATAAAATTTATTATAAAATAAGTAACACCGGTAAATTTATGAAACCAGTATAATTAAAATAAATATATAATAC
CACTTAGGAGGTTACACAAT

Downstream 100 bases:

>100_bases
TGGGCCTGAGTTTCAACGAATTTAAAGATGAAGCAATTAAAATTCTGAACATTGATCTTGATAGTTATAAAATAAAAAGG
GTAAAAAGGCGGACCGACAG

Product: response regulator receiver modulated CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MIKVMVVDDSAFIRQIMKKTINQEDDIEVIAVARNGQECLDLIEEKHPDVITLDIDMPGKDGLEILKVLMDKYSNIPVIM
LSAIDNRDTVMKALKLGAFDFIPKPSGNISLNINDVSKQLITKIKAAAGSNISCSKQGKQKATEFDHKPGDFPVIAIGSS
SGGPKALKEILPVFPNNLKAALVVVQHMPPGFTASLAKRLHQESKITVKEASEYDYLQPGVALIAPGGYHLEFNPEGRVV
LNQKPPKWGVRPCVDYMLKSVADIYKERVIGIILTGMGRDGAEGMKQVKKYNGYGIVEDKSTALVYGMPGATIEARAYDE
ILPLHQIPLKIIDLIERRK

Sequences:

>Translated_339_residues
MIKVMVVDDSAFIRQIMKKTINQEDDIEVIAVARNGQECLDLIEEKHPDVITLDIDMPGKDGLEILKVLMDKYSNIPVIM
LSAIDNRDTVMKALKLGAFDFIPKPSGNISLNINDVSKQLITKIKAAAGSNISCSKQGKQKATEFDHKPGDFPVIAIGSS
SGGPKALKEILPVFPNNLKAALVVVQHMPPGFTASLAKRLHQESKITVKEASEYDYLQPGVALIAPGGYHLEFNPEGRVV
LNQKPPKWGVRPCVDYMLKSVADIYKERVIGIILTGMGRDGAEGMKQVKKYNGYGIVEDKSTALVYGMPGATIEARAYDE
ILPLHQIPLKIIDLIERRK
>Mature_339_residues
MIKVMVVDDSAFIRQIMKKTINQEDDIEVIAVARNGQECLDLIEEKHPDVITLDIDMPGKDGLEILKVLMDKYSNIPVIM
LSAIDNRDTVMKALKLGAFDFIPKPSGNISLNINDVSKQLITKIKAAAGSNISCSKQGKQKATEFDHKPGDFPVIAIGSS
SGGPKALKEILPVFPNNLKAALVVVQHMPPGFTASLAKRLHQESKITVKEASEYDYLQPGVALIAPGGYHLEFNPEGRVV
LNQKPPKWGVRPCVDYMLKSVADIYKERVIGIILTGMGRDGAEGMKQVKKYNGYGIVEDKSTALVYGMPGATIEARAYDE
ILPLHQIPLKIIDLIERRK

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=349, Percent_Identity=35.5300859598854, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1790299, Length=102, Percent_Identity=37.2549019607843, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082052, Length=105, Percent_Identity=34.2857142857143, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 37287; Mature: 37287

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVMVVDDSAFIRQIMKKTINQEDDIEVIAVARNGQECLDLIEEKHPDVITLDIDMPGK
CEEEEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCC
DGLEILKVLMDKYSNIPVIMLSAIDNRDTVMKALKLGAFDFIPKPSGNISLNINDVSKQL
CHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHH
ITKIKAAAGSNISCSKQGKQKATEFDHKPGDFPVIAIGSSSGGPKALKEILPVFPNNLKA
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEE
ALVVVQHMPPGFTASLAKRLHQESKITVKEASEYDYLQPGVALIAPGGYHLEFNPEGRVV
EEEEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCEE
LNQKPPKWGVRPCVDYMLKSVADIYKERVIGIILTGMGRDGAEGMKQVKKYNGYGIVEDK
ECCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCEEEECC
STALVYGMPGATIEARAYDEILPLHQIPLKIIDLIERRK
CCEEEEECCCCEEEHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKVMVVDDSAFIRQIMKKTINQEDDIEVIAVARNGQECLDLIEEKHPDVITLDIDMPGK
CEEEEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCC
DGLEILKVLMDKYSNIPVIMLSAIDNRDTVMKALKLGAFDFIPKPSGNISLNINDVSKQL
CHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHH
ITKIKAAAGSNISCSKQGKQKATEFDHKPGDFPVIAIGSSSGGPKALKEILPVFPNNLKA
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEE
ALVVVQHMPPGFTASLAKRLHQESKITVKEASEYDYLQPGVALIAPGGYHLEFNPEGRVV
EEEEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCEE
LNQKPPKWGVRPCVDYMLKSVADIYKERVIGIILTGMGRDGAEGMKQVKKYNGYGIVEDK
ECCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCEEEECC
STALVYGMPGATIEARAYDEILPLHQIPLKIIDLIERRK
CCEEEEECCCCEEEHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]