Definition Halothermothrix orenii H 168 chromosome, complete genome.
Accession NC_011899
Length 2,578,146

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The map label for this gene is rnjA [H]

Identifier: 220932388

GI number: 220932388

Start: 1662349

End: 1664019

Strand: Direct

Name: rnjA [H]

Synonym: Hore_15520

Alternate gene names: 220932388

Gene position: 1662349-1664019 (Clockwise)

Preceding gene: 220932363

Following gene: 220932398

Centisome position: 64.48

GC content: 44.05

Gene sequence:

>1671_bases
ATGCCACAGAAAATTTCTATAATTCCTCTGGGTGGTTGTCGGGAAATAGGAAATAATATGATAGTTATTGAATCTAAAAA
AGACATCATAATCCTGGATTCAGGGGTGTTATTTCCCGAGGATGATGACTTCGGGTATGAACTTATAATTCCTGATATAT
CCTACCTGGTTGAAAGAAAAAAGAAAATACGGGGTATAATCTTCTCACATGGCCATGAAGACCACATTGGGGCTGTCCCG
TTTCTCCTGGAGAGAATCAATCCTCCTTTGTTTGGCACTGACCTGACGATGGGATTGATAAAAAGCAAGCTGGAAGACGC
CGGCCTTATAGACAGGGCCAGGCTAAAAACTGTCAATCACAGGAGCAAATTAATCCTGGGGGATTTTGAACTGGACTTTT
GCCATGTCAACCACAGTATCCCGGGGGCAGTGGCCACCAGGATTAAAACCCCGGTAGGTTATATTGTTTACACCGGTGAT
TATAAATTTGATCAGACCCCTGTCAACACCGGACAGACCGATTATCAGAAACTATCTGACTGGGGAAAAGAGGGAATCAG
GGTCCTGCTGGGGGATAGTACCAATGCTGAACGGGAAGGGCACAGCCTTTCAGAACGGGTTGTGGCTCAAAACATCGAAG
AAGGATTTAAGCTTATAAACGGGAGAATTATTGTAGCTACCTTCTCAACCAATATCCACCGCATCCAGCACATCATATCA
GCCGCCCGGAAGACCGGCCGAAAGGTTGCAGTGGCCGGAAGGAGTATGTTCAATACTATTCAGGTAGCTGCTGACCTTGG
TTATCTGGATATTCCACCAGGACTGTTAATAACCCTGGATGAAGCCAATAAAATGGAACCTGGTAATATAGTCCTCCTGA
TGACCGGGAGCCAGGGTGAATATGGAGCAGCCCTGACCAGGCTGTCCCGTGGAGACCACCGTGATATGGAAATCAAACCC
GGGGACACGGTTTTTATGTCAGCCAGTCCCATTCCGGGTAATGAAAAAGCCATCGGAAATACCATTAACAGGCTTTATAG
CCTGGGGGCAGAGGTTATCTATAATGGAATGCTGGATATTCATGCCTCCGGTCATGCCTGCCAGGAAGAAATAAAACTTA
TGATAAATCTGACAGGACCTGATTACCTGGTCCCGGTCCATGGTGAATTCCGCCATCTATTTCACCATGCCCAGCTGGCA
CGACAGGCCGGGGTTCCCGTCCAAAATATTTTAATCCCGGACAATGGCCAGAAGATTGATGTTTTTCCTAAAAGGGTAAA
AATGGGAAATAAAATTGAGGTTAATAAAACCCTTATTGAGGGTTATCGGGTCTGTGACAATGGAAAGGAAATACTGGGAG
AAAGAAAAAAAATGGCAAGACAGGGGTATATAAATATCCTTATGTTTGTCAATAAAAACGGTGAATTAAAAAATAAACCG
GTTATTATTTCCCGGGGCTTTACCAGCAATCGTGACTCCCGGGAACTCTTAAAAAAGGTTAAAGAAAAAGTCCAGAAACT
TATTGAAGAATTTCAAAATAACAGGCACGGTGACCCAGCTGACCTGAAATCAAGGGTCAACTATGAAGTCAATAACCTGA
TATATGATAAATTAAAGAGGGCCCCCCTGATTACACCGTTTATTATCCAGGTTAATCAGGATAGACGTTAA

Upstream 100 bases:

>100_bases
TATATTTCTTGCCCCGGGCACCCTTTTCCTGCCTCAACAATATAATTAAATATATCTAAAATGAATATAATAATAATCAG
ACTATAAGGGGGTTTTAGAT

Downstream 100 bases:

>100_bases
ATAATAGACCTTAAATATAGAGGTCGAGTAAAAGACCCCTGATCTCATCTATTTTTTGAACTATTTTTAAGACATCGGCC
TGCTCCTCCATGAACATCTC

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MPQKISIIPLGGCREIGNNMIVIESKKDIIILDSGVLFPEDDDFGYELIIPDISYLVERKKKIRGIIFSHGHEDHIGAVP
FLLERINPPLFGTDLTMGLIKSKLEDAGLIDRARLKTVNHRSKLILGDFELDFCHVNHSIPGAVATRIKTPVGYIVYTGD
YKFDQTPVNTGQTDYQKLSDWGKEGIRVLLGDSTNAEREGHSLSERVVAQNIEEGFKLINGRIIVATFSTNIHRIQHIIS
AARKTGRKVAVAGRSMFNTIQVAADLGYLDIPPGLLITLDEANKMEPGNIVLLMTGSQGEYGAALTRLSRGDHRDMEIKP
GDTVFMSASPIPGNEKAIGNTINRLYSLGAEVIYNGMLDIHASGHACQEEIKLMINLTGPDYLVPVHGEFRHLFHHAQLA
RQAGVPVQNILIPDNGQKIDVFPKRVKMGNKIEVNKTLIEGYRVCDNGKEILGERKKMARQGYINILMFVNKNGELKNKP
VIISRGFTSNRDSRELLKKVKEKVQKLIEEFQNNRHGDPADLKSRVNYEVNNLIYDKLKRAPLITPFIIQVNQDRR

Sequences:

>Translated_556_residues
MPQKISIIPLGGCREIGNNMIVIESKKDIIILDSGVLFPEDDDFGYELIIPDISYLVERKKKIRGIIFSHGHEDHIGAVP
FLLERINPPLFGTDLTMGLIKSKLEDAGLIDRARLKTVNHRSKLILGDFELDFCHVNHSIPGAVATRIKTPVGYIVYTGD
YKFDQTPVNTGQTDYQKLSDWGKEGIRVLLGDSTNAEREGHSLSERVVAQNIEEGFKLINGRIIVATFSTNIHRIQHIIS
AARKTGRKVAVAGRSMFNTIQVAADLGYLDIPPGLLITLDEANKMEPGNIVLLMTGSQGEYGAALTRLSRGDHRDMEIKP
GDTVFMSASPIPGNEKAIGNTINRLYSLGAEVIYNGMLDIHASGHACQEEIKLMINLTGPDYLVPVHGEFRHLFHHAQLA
RQAGVPVQNILIPDNGQKIDVFPKRVKMGNKIEVNKTLIEGYRVCDNGKEILGERKKMARQGYINILMFVNKNGELKNKP
VIISRGFTSNRDSRELLKKVKEKVQKLIEEFQNNRHGDPADLKSRVNYEVNNLIYDKLKRAPLITPFIIQVNQDRR
>Mature_555_residues
PQKISIIPLGGCREIGNNMIVIESKKDIIILDSGVLFPEDDDFGYELIIPDISYLVERKKKIRGIIFSHGHEDHIGAVPF
LLERINPPLFGTDLTMGLIKSKLEDAGLIDRARLKTVNHRSKLILGDFELDFCHVNHSIPGAVATRIKTPVGYIVYTGDY
KFDQTPVNTGQTDYQKLSDWGKEGIRVLLGDSTNAEREGHSLSERVVAQNIEEGFKLINGRIIVATFSTNIHRIQHIISA
ARKTGRKVAVAGRSMFNTIQVAADLGYLDIPPGLLITLDEANKMEPGNIVLLMTGSQGEYGAALTRLSRGDHRDMEIKPG
DTVFMSASPIPGNEKAIGNTINRLYSLGAEVIYNGMLDIHASGHACQEEIKLMINLTGPDYLVPVHGEFRHLFHHAQLAR
QAGVPVQNILIPDNGQKIDVFPKRVKMGNKIEVNKTLIEGYRVCDNGKEILGERKKMARQGYINILMFVNKNGELKNKPV
IISRGFTSNRDSRELLKKVKEKVQKLIEEFQNNRHGDPADLKSRVNYEVNNLIYDKLKRAPLITPFIIQVNQDRR

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 62230; Mature: 62099

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQKISIIPLGGCREIGNNMIVIESKKDIIILDSGVLFPEDDDFGYELIIPDISYLVERK
CCCEEEEEECCCHHHHCCCEEEEECCCCEEEEECCEECCCCCCCCEEEECCCHHHHHHHH
KKIRGIIFSHGHEDHIGAVPFLLERINPPLFGTDLTMGLIKSKLEDAGLIDRARLKTVNH
HHHCEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCC
RSKLILGDFELDFCHVNHSIPGAVATRIKTPVGYIVYTGDYKFDQTPVNTGQTDYQKLSD
CCEEEEEECEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHH
WGKEGIRVLLGDSTNAEREGHSLSERVVAQNIEEGFKLINGRIIVATFSTNIHRIQHIIS
HCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHH
AARKTGRKVAVAGRSMFNTIQVAADLGYLDIPPGLLITLDEANKMEPGNIVLLMTGSQGE
HHHHCCCEEEEECHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCEEEEEECCCCC
YGAALTRLSRGDHRDMEIKPGDTVFMSASPIPGNEKAIGNTINRLYSLGAEVIYNGMLDI
HHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHCCCEEE
HASGHACQEEIKLMINLTGPDYLVPVHGEFRHLFHHAQLARQAGVPVQNILIPDNGQKID
ECCCCHHHCEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCEEE
VFPKRVKMGNKIEVNKTLIEGYRVCDNGKEILGERKKMARQGYINILMFVNKNGELKNKP
CCHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
VIISRGFTSNRDSRELLKKVKEKVQKLIEEFQNNRHGDPADLKSRVNYEVNNLIYDKLKR
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHC
APLITPFIIQVNQDRR
CCCCCEEEEEECCCCC
>Mature Secondary Structure 
PQKISIIPLGGCREIGNNMIVIESKKDIIILDSGVLFPEDDDFGYELIIPDISYLVERK
CCEEEEEECCCHHHHCCCEEEEECCCCEEEEECCEECCCCCCCCEEEECCCHHHHHHHH
KKIRGIIFSHGHEDHIGAVPFLLERINPPLFGTDLTMGLIKSKLEDAGLIDRARLKTVNH
HHHCEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCC
RSKLILGDFELDFCHVNHSIPGAVATRIKTPVGYIVYTGDYKFDQTPVNTGQTDYQKLSD
CCEEEEEECEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHH
WGKEGIRVLLGDSTNAEREGHSLSERVVAQNIEEGFKLINGRIIVATFSTNIHRIQHIIS
HCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHH
AARKTGRKVAVAGRSMFNTIQVAADLGYLDIPPGLLITLDEANKMEPGNIVLLMTGSQGE
HHHHCCCEEEEECHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCEEEEEECCCCC
YGAALTRLSRGDHRDMEIKPGDTVFMSASPIPGNEKAIGNTINRLYSLGAEVIYNGMLDI
HHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHCCCEEE
HASGHACQEEIKLMINLTGPDYLVPVHGEFRHLFHHAQLARQAGVPVQNILIPDNGQKID
ECCCCHHHCEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCEEE
VFPKRVKMGNKIEVNKTLIEGYRVCDNGKEILGERKKMARQGYINILMFVNKNGELKNKP
CCHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
VIISRGFTSNRDSRELLKKVKEKVQKLIEEFQNNRHGDPADLKSRVNYEVNNLIYDKLKR
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHC
APLITPFIIQVNQDRR
CCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]