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The map label for this gene is ilvB [H]

Identifier: 220931924

GI number: 220931924

Start: 1176553

End: 1178220

Strand: Direct

Name: ilvB [H]

Synonym: Hore_10830

Alternate gene names: 220931924

Gene position: 1176553-1178220 (Clockwise)

Preceding gene: 220931923

Following gene: 220931925

Centisome position: 45.64

GC content: 40.17

Gene sequence:

>1668_bases
ATGTCACTTATGACCGGGGCTGAAATTGTTGTTCAATCTCTCCTTAAAGAGAAGGTTAAAGATATTTTTGGTTATCCAGG
AGGAGCGGTATTACCTATATATGATGTTTTATATGATTCCCCTATAAATCATTATCTTACCAGGCACGAACAGGGAGCCA
TACATGCAGCTGATGGTTATGCCCGCAGTACCGGAGAGGTTGGTGTCTGTATAGCTACCTCTGGTCCAGGGAGTACCAAC
CTGGTTACCGGTCTGGCAACAGCTTATATGGATTCTGTACCTGTAGTTGCCTTTACAGGTCAGGTAACTACTAATTTTAT
AGGAAAAGACGCCTTCCAGGAGGCTGATATAAGGGGAATAACTATTCCTATAACCAAACACAATTACCTGGTTACTGATA
TTCAAGACCTGGCCAGAACCATAAAAGAAGCCTTTTATCTGGCCAGAACGGGAAGGCCGGGTCCTGTATTAATAGATATC
CCTAAAGATATTACCAGGGATAAATGTGAATTTGTTTATCCAGATAAAGTTGAGCTTCCGGGTTATGATCCAACCTACAG
GGGACATAAACTACAGATTAACAAGGCTGCCCATGAAATAAATAAAGCAGAGAAGCCTGTTATTTTTGCCGGAGGTGGAG
TAATAGCCTCAGAGGCCTCAACAGAATTAAGAAAACTGGTTAAAAAGTCCAGTATTCCGATTACAACTTCATTAATGGGG
CTTGGTATATATCCTGAAAATGATTTTTTGTCTCTGGGAATGCCGGGTATGCATGGTACCAGGTATGCCAATTATGCTAT
ATCTGAAGCTGACCTTTTAGTAGCTGTTGGGGTCAGGTTTGATGACAGGGTTACCGGTAAGATAGAAGAATTCGCTCCAG
ACGCAAAAATAATTCATATTGATATTGACCCGGCTGAGATATGTAAAAATGTTGAAGCCCATATACCCATTGTAGGGGAT
GCCAGAAATATTTTAAAAGAATTATTACCCCTTATCGAGCATAAAGAACGTGGTGCCTGGCTTCAGCAAATTAAAGAATG
GAAGGAAAAAAATCCATTAAAATATGAACAGAGTGAAACCAATATAAAACCACAATATGTTATTGAAAAATTATATGAAC
TGACCGGTGGAGAGGCAATAATCTCTACTGAGGTAGGTCAAAATCAAATGTGGGCTGCCCAGTATTATAAGTACAGTCAC
CCTAGAACATTCTTATCATCCGGTGGACTGGGAACCATGGGTTATGGCTTTCCAGCAGCTATAGGGGCTCAGGTTGGTAA
CCCGGACAGGGTTGTTATTGATATTGCCGGTGATGGTAGTATCCAGATGAACATCCAGGAACTGGCTACTGTTAGTAATT
ATAATTTACCTGTAAAAATAATTATCCTCAATAATAGTTATCTGGGTATGGTCCGGCAGTGGCAGGAATTATTCTATGAT
AAAAGGTATTCATCCACAGGCCTAAAAAATCCAGATTTTATTAAACTGGCTGAAGCTTACGGTGTCTATGCCCTAAAAAT
AGATAAAAAAGATGAGGTGAAACCGGCTTTAGAAAAGATCCTATCGGTTAATGGCCCTGCTTTACTGGATGTCAGAATTG
AGGAAGAAGAAAATGTATTTCCCATGGTACCACAGGGAGCAGGAATTAAAAAAATGATAGGAGGTTAA

Upstream 100 bases:

>100_bases
GAAGGGTATTAACAATGCTTATTTTTCTATGTAATAGATAACCCTGGGTTAACTACACCCAGGGTTTTCTTATATTCACA
GGAAATGGAGGTGGGATGGT

Downstream 100 bases:

>100_bases
CAGTTATGACTCATATACTTTCGGTATCTGTTTTAAATAAGCCGGGTGTTTTAACCAGAATCGCCAGCCTTTTTAGCCGG
AGGAATTTTAATATTGAAAG

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSLMTGAEIVVQSLLKEKVKDIFGYPGGAVLPIYDVLYDSPINHYLTRHEQGAIHAADGYARSTGEVGVCIATSGPGSTN
LVTGLATAYMDSVPVVAFTGQVTTNFIGKDAFQEADIRGITIPITKHNYLVTDIQDLARTIKEAFYLARTGRPGPVLIDI
PKDITRDKCEFVYPDKVELPGYDPTYRGHKLQINKAAHEINKAEKPVIFAGGGVIASEASTELRKLVKKSSIPITTSLMG
LGIYPENDFLSLGMPGMHGTRYANYAISEADLLVAVGVRFDDRVTGKIEEFAPDAKIIHIDIDPAEICKNVEAHIPIVGD
ARNILKELLPLIEHKERGAWLQQIKEWKEKNPLKYEQSETNIKPQYVIEKLYELTGGEAIISTEVGQNQMWAAQYYKYSH
PRTFLSSGGLGTMGYGFPAAIGAQVGNPDRVVIDIAGDGSIQMNIQELATVSNYNLPVKIIILNNSYLGMVRQWQELFYD
KRYSSTGLKNPDFIKLAEAYGVYALKIDKKDEVKPALEKILSVNGPALLDVRIEEEENVFPMVPQGAGIKKMIGG

Sequences:

>Translated_555_residues
MSLMTGAEIVVQSLLKEKVKDIFGYPGGAVLPIYDVLYDSPINHYLTRHEQGAIHAADGYARSTGEVGVCIATSGPGSTN
LVTGLATAYMDSVPVVAFTGQVTTNFIGKDAFQEADIRGITIPITKHNYLVTDIQDLARTIKEAFYLARTGRPGPVLIDI
PKDITRDKCEFVYPDKVELPGYDPTYRGHKLQINKAAHEINKAEKPVIFAGGGVIASEASTELRKLVKKSSIPITTSLMG
LGIYPENDFLSLGMPGMHGTRYANYAISEADLLVAVGVRFDDRVTGKIEEFAPDAKIIHIDIDPAEICKNVEAHIPIVGD
ARNILKELLPLIEHKERGAWLQQIKEWKEKNPLKYEQSETNIKPQYVIEKLYELTGGEAIISTEVGQNQMWAAQYYKYSH
PRTFLSSGGLGTMGYGFPAAIGAQVGNPDRVVIDIAGDGSIQMNIQELATVSNYNLPVKIIILNNSYLGMVRQWQELFYD
KRYSSTGLKNPDFIKLAEAYGVYALKIDKKDEVKPALEKILSVNGPALLDVRIEEEENVFPMVPQGAGIKKMIGG
>Mature_554_residues
SLMTGAEIVVQSLLKEKVKDIFGYPGGAVLPIYDVLYDSPINHYLTRHEQGAIHAADGYARSTGEVGVCIATSGPGSTNL
VTGLATAYMDSVPVVAFTGQVTTNFIGKDAFQEADIRGITIPITKHNYLVTDIQDLARTIKEAFYLARTGRPGPVLIDIP
KDITRDKCEFVYPDKVELPGYDPTYRGHKLQINKAAHEINKAEKPVIFAGGGVIASEASTELRKLVKKSSIPITTSLMGL
GIYPENDFLSLGMPGMHGTRYANYAISEADLLVAVGVRFDDRVTGKIEEFAPDAKIIHIDIDPAEICKNVEAHIPIVGDA
RNILKELLPLIEHKERGAWLQQIKEWKEKNPLKYEQSETNIKPQYVIEKLYELTGGEAIISTEVGQNQMWAAQYYKYSHP
RTFLSSGGLGTMGYGFPAAIGAQVGNPDRVVIDIAGDGSIQMNIQELATVSNYNLPVKIIILNNSYLGMVRQWQELFYDK
RYSSTGLKNPDFIKLAEAYGVYALKIDKKDEVKPALEKILSVNGPALLDVRIEEEENVFPMVPQGAGIKKMIGG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=557, Percent_Identity=25.3141831238779, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI21361361, Length=563, Percent_Identity=23.8010657193606, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=48.4098939929329, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=45.9312839059675, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=34.2198581560284, Blast_Score=334, Evalue=8e-93,
Organism=Escherichia coli, GI1787096, Length=547, Percent_Identity=29.7989031078611, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1788716, Length=569, Percent_Identity=26.5377855887522, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=574, Percent_Identity=27.1777003484321, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=574, Percent_Identity=27.1777003484321, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17542570, Length=572, Percent_Identity=24.1258741258741, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=46.8641114982578, Blast_Score=510, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6320816, Length=491, Percent_Identity=24.2362525458248, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=494, Percent_Identity=23.6842105263158, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=490, Percent_Identity=23.0612244897959, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=497, Percent_Identity=23.1388329979879, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320123, Length=492, Percent_Identity=22.5609756097561, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=483, Percent_Identity=25.6728778467909, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61053; Mature: 60922

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLMTGAEIVVQSLLKEKVKDIFGYPGGAVLPIYDVLYDSPINHYLTRHEQGAIHAADGY
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCEEECCCC
ARSTGEVGVCIATSGPGSTNLVTGLATAYMDSVPVVAFTGQVTTNFIGKDAFQEADIRGI
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHCCCCCEE
TIPITKHNYLVTDIQDLARTIKEAFYLARTGRPGPVLIDIPKDITRDKCEFVYPDKVELP
EEEEECCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEECCCCEECC
GYDPTYRGHKLQINKAAHEINKAEKPVIFAGGGVIASEASTELRKLVKKSSIPITTSLMG
CCCCCCCCCEEEEHHHHHHHHHCCCCEEEECCCEEECHHHHHHHHHHHHCCCCEEEHHEE
LGIYPENDFLSLGMPGMHGTRYANYAISEADLLVAVGVRFDDRVTGKIEEFAPDAKIIHI
EECCCCCCEEECCCCCCCCCEECCEEECCCCEEEEECCCCCCCCCCCHHHHCCCCEEEEE
DIDPAEICKNVEAHIPIVGDARNILKELLPLIEHKERGAWLQQIKEWKEKNPLKYEQSET
ECCHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
NIKPQYVIEKLYELTGGEAIISTEVGQNQMWAAQYYKYSHPRTFLSSGGLGTMGYGFPAA
CCCHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHCCCCHHHHHCCCCCCCCCCCHHH
IGAQVGNPDRVVIDIAGDGSIQMNIQELATVSNYNLPVKIIILNNSYLGMVRQWQELFYD
HCCCCCCCCEEEEEEECCCEEEEEHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
KRYSSTGLKNPDFIKLAEAYGVYALKIDKKDEVKPALEKILSVNGPALLDVRIEEEENVF
CCCCCCCCCCCCCEEEEHHCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCC
PMVPQGAGIKKMIGG
CCCCCCCCCHHHHCC
>Mature Secondary Structure 
SLMTGAEIVVQSLLKEKVKDIFGYPGGAVLPIYDVLYDSPINHYLTRHEQGAIHAADGY
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCEEECCCC
ARSTGEVGVCIATSGPGSTNLVTGLATAYMDSVPVVAFTGQVTTNFIGKDAFQEADIRGI
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHCCCCCEE
TIPITKHNYLVTDIQDLARTIKEAFYLARTGRPGPVLIDIPKDITRDKCEFVYPDKVELP
EEEEECCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEECCCCEECC
GYDPTYRGHKLQINKAAHEINKAEKPVIFAGGGVIASEASTELRKLVKKSSIPITTSLMG
CCCCCCCCCEEEEHHHHHHHHHCCCCEEEECCCEEECHHHHHHHHHHHHCCCCEEEHHEE
LGIYPENDFLSLGMPGMHGTRYANYAISEADLLVAVGVRFDDRVTGKIEEFAPDAKIIHI
EECCCCCCEEECCCCCCCCCEECCEEECCCCEEEEECCCCCCCCCCCHHHHCCCCEEEEE
DIDPAEICKNVEAHIPIVGDARNILKELLPLIEHKERGAWLQQIKEWKEKNPLKYEQSET
ECCHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
NIKPQYVIEKLYELTGGEAIISTEVGQNQMWAAQYYKYSHPRTFLSSGGLGTMGYGFPAA
CCCHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHCCCCHHHHHCCCCCCCCCCCHHH
IGAQVGNPDRVVIDIAGDGSIQMNIQELATVSNYNLPVKIIILNNSYLGMVRQWQELFYD
HCCCCCCCCEEEEEEECCCEEEEEHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
KRYSSTGLKNPDFIKLAEAYGVYALKIDKKDEVKPALEKILSVNGPALLDVRIEEEENVF
CCCCCCCCCCCCCEEEEHHCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCC
PMVPQGAGIKKMIGG
CCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]