Click here to switch to the map view.

The map label for this gene is nylA [H]

Identifier: 220918675

GI number: 220918675

Start: 4001570

End: 4003057

Strand: Direct

Name: nylA [H]

Synonym: A2cp1_3583

Alternate gene names: 220918675

Gene position: 4001570-4003057 (Clockwise)

Preceding gene: 220918674

Following gene: 220918679

Centisome position: 79.56

GC content: 78.56

Gene sequence:

>1488_bases
GTGCGACTGCCGGAGTACGACCGCCTCGACGCGCTCGGCCTGGCCGAGCTGGTCGCGCGCCGCGAGGTGTCCGCGCCCGA
GCTGCTGGAGGCGGCGCTGGAGCGCGCCGACCGGCGCAATCCGTCCATCAACGCCATCGTCGCCCGCTACGACGACGAGG
CCCGCGCCCGCGCCCGCGGCCCGCTCCCCGCCGGCCCGCTCTCGGGTGTCCCGTTCCTGCTGAAGGACCTGCTGGCCGCG
TGGAAGGGCCACCCGTACACCTGCTCCTCGCGGCTGCTGGCCGGGTTCGTGGCGCCGGAGGACGCGGAGACGGTGCGCCG
GTTCCAGGCGGCCGGGCTGGTGCTGTTCGGGCAGACCAACACGCCGGAGCTGGGCGTCATGGGCGTCACCGAGCCGGCGC
TGCGCGGGCCGACGCGGAACCCGTGGAACCCGGCGTTCACCTCGGGCGGCTCGTCGGGCGGGAGCGCGGCGGCGGTGGCG
GCGCGGATCGTCCCCGCCGCGCACGGCAACGACGGCGGCGGCTCGCTGCGCATCCCGGCGTCCGTGTGCGGGCTGTTCGC
GCTGAAGGCGACGCGCGGGCGCGTCACGCTGGCGCCGGCGTTCGGCGAGGCGCTGGGCGGGTTCGCGATCGACGGCGTGC
TGACGCGCTCGGTGCGCGACTCCGCGGCGCTGCTCGACGCCATCGACGGCCCGGCGCCGGGCGATCCGTACGCGGCGCCG
CCGAAGGAGCGACCGTTCCTCGCCGAGGTGGGCGCGCCGCCGGGGCGCCTGCGCATCGCGTTCACGCGCGAGGGCCTGTT
CGCGCGCTCCACCGACGCGGAGTGCGCCGCGGCGGTGGACGCGGCGGCGCGGCTGCTCGCCGACCTCGGGCACGAGCTGG
TCGAGGCGCGCCCGAAGTTCCCGCGCGACGCGCTGGCGCGGGCGTACCTGGTGACGCTCGCGGCGCAGACCGCGGCCGAC
GTCGCGCTCGCCGCCCGCCACGCGGGCCGCCGCCCGCGCGCCGGGGACCTCGAGCCGGAGACGGCCGCGCTCGCGGCGGG
CGGCCGCGCGCTCTCGGCGCAGGACCTGGTGCTGGCCCGCGTCGAGCTGGAGCAGGCCTCGCGGGGCGTGGCGGCGTTCT
TCGAGCGGCACGACGCGCTGCTCACGCCCACGCTGGCGCGCCCGCCGGTCCGGGTCGGGGCGCTGGCGGCGCGCTCGCAC
GAGCGGCTCGCGCTGCGGCTGGTGGCCCGCCTCGGCTCGCGCCGGCTGCTGGAGCGCCTCTTCGACGAGCTGGGCAGCCG
CTCGTTCGACGCCACCGGCAACACCATGCTGTTCAACCAGACCGGCCAGCCGGCGATGAGCCTGCCGCTCCACTTCAGCC
GCGAGGGCCTGCCCATCGGCGTGCAGGTGGTGGGGCGCTTCGGGGCCGAGGCGACGCTGTTCCGGCTCGCGGCGCAGGTG
GAGCAGGCCGCGCCGTGGGCCGGCCGGGCGCCGCCGCTCAGCGCGTGA

Upstream 100 bases:

>100_bases
TCCTAGCTTCCGCCCCCGGCGGGGGTGCGGAGGCCCGCGGGCACCCCGCGCCCCGGCCCGGGGCTATCCTGGGAGGTGAC
GTCCCCCGGGAGGCCACGCC

Downstream 100 bases:

>100_bases
ACGGCCAGATCAGCGGGACGCCCAGGGTGAGCAGCGCCACGATCGCGAGCGTGAGCGGGGTCCCCACGCGCACGAAGTCC
GAGAAGCGGTACCGGCCCGC

Product: Amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRLPEYDRLDALGLAELVARREVSAPELLEAALERADRRNPSINAIVARYDDEARARARGPLPAGPLSGVPFLLKDLLAA
WKGHPYTCSSRLLAGFVAPEDAETVRRFQAAGLVLFGQTNTPELGVMGVTEPALRGPTRNPWNPAFTSGGSSGGSAAAVA
ARIVPAAHGNDGGGSLRIPASVCGLFALKATRGRVTLAPAFGEALGGFAIDGVLTRSVRDSAALLDAIDGPAPGDPYAAP
PKERPFLAEVGAPPGRLRIAFTREGLFARSTDAECAAAVDAAARLLADLGHELVEARPKFPRDALARAYLVTLAAQTAAD
VALAARHAGRRPRAGDLEPETAALAAGGRALSAQDLVLARVELEQASRGVAAFFERHDALLTPTLARPPVRVGALAARSH
ERLALRLVARLGSRRLLERLFDELGSRSFDATGNTMLFNQTGQPAMSLPLHFSREGLPIGVQVVGRFGAEATLFRLAAQV
EQAAPWAGRAPPLSA

Sequences:

>Translated_495_residues
MRLPEYDRLDALGLAELVARREVSAPELLEAALERADRRNPSINAIVARYDDEARARARGPLPAGPLSGVPFLLKDLLAA
WKGHPYTCSSRLLAGFVAPEDAETVRRFQAAGLVLFGQTNTPELGVMGVTEPALRGPTRNPWNPAFTSGGSSGGSAAAVA
ARIVPAAHGNDGGGSLRIPASVCGLFALKATRGRVTLAPAFGEALGGFAIDGVLTRSVRDSAALLDAIDGPAPGDPYAAP
PKERPFLAEVGAPPGRLRIAFTREGLFARSTDAECAAAVDAAARLLADLGHELVEARPKFPRDALARAYLVTLAAQTAAD
VALAARHAGRRPRAGDLEPETAALAAGGRALSAQDLVLARVELEQASRGVAAFFERHDALLTPTLARPPVRVGALAARSH
ERLALRLVARLGSRRLLERLFDELGSRSFDATGNTMLFNQTGQPAMSLPLHFSREGLPIGVQVVGRFGAEATLFRLAAQV
EQAAPWAGRAPPLSA
>Mature_495_residues
MRLPEYDRLDALGLAELVARREVSAPELLEAALERADRRNPSINAIVARYDDEARARARGPLPAGPLSGVPFLLKDLLAA
WKGHPYTCSSRLLAGFVAPEDAETVRRFQAAGLVLFGQTNTPELGVMGVTEPALRGPTRNPWNPAFTSGGSSGGSAAAVA
ARIVPAAHGNDGGGSLRIPASVCGLFALKATRGRVTLAPAFGEALGGFAIDGVLTRSVRDSAALLDAIDGPAPGDPYAAP
PKERPFLAEVGAPPGRLRIAFTREGLFARSTDAECAAAVDAAARLLADLGHELVEARPKFPRDALARAYLVTLAAQTAAD
VALAARHAGRRPRAGDLEPETAALAAGGRALSAQDLVLARVELEQASRGVAAFFERHDALLTPTLARPPVRVGALAARSH
ERLALRLVARLGSRRLLERLFDELGSRSFDATGNTMLFNQTGQPAMSLPLHFSREGLPIGVQVVGRFGAEATLFRLAAQV
EQAAPWAGRAPPLSA

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=520, Percent_Identity=26.9230769230769, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI195972892, Length=240, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=212, Percent_Identity=31.6037735849057, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=308, Percent_Identity=30.8441558441558, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=310, Percent_Identity=27.741935483871, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=173, Percent_Identity=35.8381502890173, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=239, Percent_Identity=31.3807531380753, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556276, Length=239, Percent_Identity=31.3807531380753, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=175, Percent_Identity=33.1428571428571, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648113, Length=450, Percent_Identity=31.5555555555556, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24644968, Length=514, Percent_Identity=26.2645914396887, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45550774, Length=244, Percent_Identity=30.7377049180328, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648435, Length=244, Percent_Identity=30.7377049180328, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648437, Length=244, Percent_Identity=30.7377049180328, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356731, Length=238, Percent_Identity=30.672268907563, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI161078093, Length=537, Percent_Identity=24.2085661080074, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24652985, Length=211, Percent_Identity=28.436018957346, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922090, Length=211, Percent_Identity=28.436018957346, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652981, Length=211, Percent_Identity=28.436018957346, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652983, Length=211, Percent_Identity=28.436018957346, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648441, Length=175, Percent_Identity=30.2857142857143, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24648439, Length=175, Percent_Identity=30.2857142857143, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 51950; Mature: 51950

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPEYDRLDALGLAELVARREVSAPELLEAALERADRRNPSINAIVARYDDEARARARG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCC
PLPAGPLSGVPFLLKDLLAAWKGHPYTCSSRLLAGFVAPEDAETVRRFQAAGLVLFGQTN
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCCC
TPELGVMGVTEPALRGPTRNPWNPAFTSGGSSGGSAAAVAARIVPAAHGNDGGGSLRIPA
CCCCEEECCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECHH
SVCGLFALKATRGRVTLAPAFGEALGGFAIDGVLTRSVRDSAALLDAIDGPAPGDPYAAP
HHHHHHHHHCCCCCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKERPFLAEVGAPPGRLRIAFTREGLFARSTDAECAAAVDAAARLLADLGHELVEARPKF
CCCCCEEEECCCCCCCEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRDALARAYLVTLAAQTAADVALAARHAGRRPRAGDLEPETAALAAGGRALSAQDLVLAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHH
VELEQASRGVAAFFERHDALLTPTLARPPVRVGALAARSHERLALRLVARLGSRRLLERL
HHHHHHHCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDELGSRSFDATGNTMLFNQTGQPAMSLPLHFSREGLPIGVQVVGRFGAEATLFRLAAQV
HHHHCCCCCCCCCCEEEEECCCCCCEECCCEECCCCCCCHHHHHHHCCCHHHHHHHHHHH
EQAAPWAGRAPPLSA
HHCCCCCCCCCCCCC
>Mature Secondary Structure
MRLPEYDRLDALGLAELVARREVSAPELLEAALERADRRNPSINAIVARYDDEARARARG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCC
PLPAGPLSGVPFLLKDLLAAWKGHPYTCSSRLLAGFVAPEDAETVRRFQAAGLVLFGQTN
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCCC
TPELGVMGVTEPALRGPTRNPWNPAFTSGGSSGGSAAAVAARIVPAAHGNDGGGSLRIPA
CCCCEEECCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECHH
SVCGLFALKATRGRVTLAPAFGEALGGFAIDGVLTRSVRDSAALLDAIDGPAPGDPYAAP
HHHHHHHHHCCCCCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKERPFLAEVGAPPGRLRIAFTREGLFARSTDAECAAAVDAAARLLADLGHELVEARPKF
CCCCCEEEECCCCCCCEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRDALARAYLVTLAAQTAADVALAARHAGRRPRAGDLEPETAALAAGGRALSAQDLVLAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHH
VELEQASRGVAAFFERHDALLTPTLARPPVRVGALAARSHERLALRLVARLGSRRLLERL
HHHHHHHCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDELGSRSFDATGNTMLFNQTGQPAMSLPLHFSREGLPIGVQVVGRFGAEATLFRLAAQV
HHHHCCCCCCCCCCEEEEECCCCCCEECCCEECCCCCCCHHHHHHHCCCHHHHHHHHHHH
EQAAPWAGRAPPLSA
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]