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The map label for this gene is pucG [H]

Identifier: 220918291

GI number: 220918291

Start: 3538083

End: 3539273

Strand: Direct

Name: pucG [H]

Synonym: A2cp1_3194

Alternate gene names: 220918291

Gene position: 3538083-3539273 (Clockwise)

Preceding gene: 220918289

Following gene: 220918293

Centisome position: 70.35

GC content: 73.97

Gene sequence:

>1191_bases
ATGAGCACCCCCGTCGTCTCCGAGCTGGCGCCTCCCCTGCGCCTGCTCCTCGGCCCCGGTCCCAGCATGGTCCACCCGCG
CGTGCTCCGCGCGATGTCCACCCCGCTGCTCGGCCACCTCGATCCGAAGTTCCTCGAGATCATGAACGAGGTGCAGGCGC
AGCTCCGCGCCGTGTTCCGCACCGAGAACCCGTTCACCATCGCGGTGTCGGGCACGGGCTCGGCCGGCATGGAGGCGGCG
CTCGTCAACCTGCTCGAGCCGGGCGACACCGCCGTGGTGGTGGTGGCGGGCGTGTTCGGCGGGCGCATGGCGGACATCGT
GGGCCGCTGCGGCGCCCGGCTGGTGAAGATCGACGTGCCCTGGGGCGAGGTGTGCGATCCGGCCCGCATCGAGGAGGCGC
TGAAGCGGGAGGGCAAGGTGAAGGTGGTGGCGCTGGTCCACGCCGAGACCTCCACCGGCGCGCACCAGCCGCTCGAGGGG
CTGGGCGCCCTGTGCCACGCGCACGGCGCGCTGCTCGTCGCGGACACGGTCACCTCGCTCGGCGGCGTGCCGGTCGAGGT
GGACGCCTGGGGCGTGGACGCGTGCTACTCCGGCACGCAGAAGTGCCTGTCCTGCCCGCCCGGGCTGGCCCCGCTGACGC
TCTCGCCGCGCGCGCTCGAGGCGGTGAAGGCGCGCAAGACCAAGGTGCAGAGCTGGTACCTCGACGCCGGCATGGTGGCG
GACTACTGGGCCGAGGGGAAGCGCGTGTACCACCACACCGCGCCCATCTCGATGGTGTACGCGCTCCGCGAGTCGCTCCG
CCTGGTGCTCGAGGAGGGGCTCGAGGCGCGCTTCGCGCGGCACCGCCGCCACTCCGCCGCGCTCATGGCCGGCGTCGCCG
CGCTCGGGTTCGAGCCGCAGGCGCAGGAGGGTCACCGCCTCCCGTCGCTGAACTGCATCAAGGTGCCGGCCGGCGTCGAC
GAGGCGGCCGCGCGCAAGGGCCTGCTCGCCGATCACTCCATCGAGATCGGCGGCGGCCTCGGCCCGCTCGCGGGCAAGGT
GTGGCGCATCGGGCTCATGGGCGAGTCGGCCCGGCAGGAGAACGTGCTCGCGGTGCTCGCCGCGATCGAGCAGGCGCTCG
CGAAGCAGGGCAAGCTCGCCCGCGCCGGCGCGTCGCTCGCCGCCGCGCTCGAGTCCTACGCGCGGTCGTAG

Upstream 100 bases:

>100_bases
GCGAATCCCCTCCCGGGGTCGCGCGGGCCGCCGGCGATCGCGTAAGCTCGTCGACCTCCTCTCCCCAGGCCGGCCCCGCG
CCGGCGCCCACGAGGTCCCC

Downstream 100 bases:

>100_bases
GCGGACGTCGCCGCGGGGTGTGCCGGGAGGAGGTGCATCCCGCGGCGCTACGCAGGCTGTACGAGGGTCAGCGCCGGCAC
CGGTACCCGCGCCAGCAACC

Product: class V aminotransferase

Products: pyruvate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSTPVVSELAPPLRLLLGPGPSMVHPRVLRAMSTPLLGHLDPKFLEIMNEVQAQLRAVFRTENPFTIAVSGTGSAGMEAA
LVNLLEPGDTAVVVVAGVFGGRMADIVGRCGARLVKIDVPWGEVCDPARIEEALKREGKVKVVALVHAETSTGAHQPLEG
LGALCHAHGALLVADTVTSLGGVPVEVDAWGVDACYSGTQKCLSCPPGLAPLTLSPRALEAVKARKTKVQSWYLDAGMVA
DYWAEGKRVYHHTAPISMVYALRESLRLVLEEGLEARFARHRRHSAALMAGVAALGFEPQAQEGHRLPSLNCIKVPAGVD
EAAARKGLLADHSIEIGGGLGPLAGKVWRIGLMGESARQENVLAVLAAIEQALAKQGKLARAGASLAAALESYARS

Sequences:

>Translated_396_residues
MSTPVVSELAPPLRLLLGPGPSMVHPRVLRAMSTPLLGHLDPKFLEIMNEVQAQLRAVFRTENPFTIAVSGTGSAGMEAA
LVNLLEPGDTAVVVVAGVFGGRMADIVGRCGARLVKIDVPWGEVCDPARIEEALKREGKVKVVALVHAETSTGAHQPLEG
LGALCHAHGALLVADTVTSLGGVPVEVDAWGVDACYSGTQKCLSCPPGLAPLTLSPRALEAVKARKTKVQSWYLDAGMVA
DYWAEGKRVYHHTAPISMVYALRESLRLVLEEGLEARFARHRRHSAALMAGVAALGFEPQAQEGHRLPSLNCIKVPAGVD
EAAARKGLLADHSIEIGGGLGPLAGKVWRIGLMGESARQENVLAVLAAIEQALAKQGKLARAGASLAAALESYARS
>Mature_395_residues
STPVVSELAPPLRLLLGPGPSMVHPRVLRAMSTPLLGHLDPKFLEIMNEVQAQLRAVFRTENPFTIAVSGTGSAGMEAAL
VNLLEPGDTAVVVVAGVFGGRMADIVGRCGARLVKIDVPWGEVCDPARIEEALKREGKVKVVALVHAETSTGAHQPLEGL
GALCHAHGALLVADTVTSLGGVPVEVDAWGVDACYSGTQKCLSCPPGLAPLTLSPRALEAVKARKTKVQSWYLDAGMVAD
YWAEGKRVYHHTAPISMVYALRESLRLVLEEGLEARFARHRRHSAALMAGVAALGFEPQAQEGHRLPSLNCIKVPAGVDE
AAARKGLLADHSIEIGGGLGPLAGKVWRIGLMGESARQENVLAVLAAIEQALAKQGKLARAGASLAAALESYARS

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=372, Percent_Identity=41.6666666666667, Blast_Score=284, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17536281, Length=370, Percent_Identity=44.5945945945946, Blast_Score=304, Evalue=6e-83,
Organism=Saccharomyces cerevisiae, GI6321079, Length=377, Percent_Identity=27.5862068965517, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17530823, Length=369, Percent_Identity=40.9214092140921, Blast_Score=278, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.44

Molecular weight: Translated: 41647; Mature: 41516

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPVVSELAPPLRLLLGPGPSMVHPRVLRAMSTPLLGHLDPKFLEIMNEVQAQLRAVFR
CCCCCHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
TENPFTIAVSGTGSAGMEAALVNLLEPGDTAVVVVAGVFGGRMADIVGRCGARLVKIDVP
CCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEHHCCHHHHHHHHCCCEEEEEECC
WGEVCDPARIEEALKREGKVKVVALVHAETSTGAHQPLEGLGALCHAHGALLVADTVTSL
CCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHH
GGVPVEVDAWGVDACYSGTQKCLSCPPGLAPLTLSPRALEAVKARKTKVQSWYLDAGMVA
CCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHEECCCHHH
DYWAEGKRVYHHTAPISMVYALRESLRLVLEEGLEARFARHRRHSAALMAGVAALGFEPQ
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AQEGHRLPSLNCIKVPAGVDEAAARKGLLADHSIEIGGGLGPLAGKVWRIGLMGESARQE
CCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCEEECCCCCCCCHHHEEEEECCCCCHHH
NVLAVLAAIEQALAKQGKLARAGASLAAALESYARS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STPVVSELAPPLRLLLGPGPSMVHPRVLRAMSTPLLGHLDPKFLEIMNEVQAQLRAVFR
CCCCHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
TENPFTIAVSGTGSAGMEAALVNLLEPGDTAVVVVAGVFGGRMADIVGRCGARLVKIDVP
CCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEHHCCHHHHHHHHCCCEEEEEECC
WGEVCDPARIEEALKREGKVKVVALVHAETSTGAHQPLEGLGALCHAHGALLVADTVTSL
CCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHH
GGVPVEVDAWGVDACYSGTQKCLSCPPGLAPLTLSPRALEAVKARKTKVQSWYLDAGMVA
CCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHEECCCHHH
DYWAEGKRVYHHTAPISMVYALRESLRLVLEEGLEARFARHRRHSAALMAGVAALGFEPQ
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AQEGHRLPSLNCIKVPAGVDEAAARKGLLADHSIEIGGGLGPLAGKVWRIGLMGESARQE
CCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCEEECCCCCCCCHHHEEEEECCCCCHHH
NVLAVLAAIEQALAKQGKLARAGASLAAALESYARS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-alanine; glyoxylate

Specific reaction: L-alanine + glyoxylate = pyruvate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]