BacMap

Definition	Anaeromyxobacter dehalogenans 2CP-1 chromosome, complete genome.
Accession	NC_011891
Length	5,029,329

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The map label for this gene is yogA [H]

Identifier: 220918124

GI number: 220918124

Start: 3351524

End: 3352552

Strand: Reverse

Name: yogA [H]

Synonym: A2cp1_3027

Alternate gene names: 220918124

Gene position: 3352552-3351524 (Counterclockwise)

Preceding gene: 220918125

Following gene: 220918123

Centisome position: 66.66

GC content: 74.93

Gene sequence:

>1029_bases
ATGAAGGCGGTCCTGATCCGCGGCCACGGCGGCCCAGAGCGCGTCGAGGTCGGCGAGCTTCCGCTGCCGGAGCCCGGCCC
GGGCGAGGTCCGGGTGCGGGTGCGCGCAGCGGCGCTCAACCACCTCGACCTCTGGGTCCGCAAGGGCTGGAGCGGCCTCA
TGCTGTCGTTCCCGCACGTGCTCGGCTCGGACGTGGCCGGCGTGGTGGACGCGGTGGGGCCCGGGGTGGACGAGCCGAAG
GTGGGGGCGGAGGTGGTGCTCGCGCCCGGGCTCTCCTGCGGGCGCTGCGTGCGCTGCCGCTCCGGCGAGGAGAACCTGTG
CCGCGGCTACGCCATCCTGGGCGAGCACGTCTCCGGCGGCCAGGCCGAGGCGGTGGTGGTCCCGGCCCGCAACGCGCTGC
CGCTCCCGCCGAACCTGTCCTTCGAGGAGGCGGCGGCGGTGCCGCTCACGTTCCTCACCGCCTGGCACGCGCTGGTGGCG
CGCGCACGCGTGCGGCCCGGCGAGACGGTGCTGGTGCACGCGGCCGGCTCGGGCGTCGGCGTCGCGGCGGTGCAGATCGC
GAAGCTGCACGGGGCGCGCGTCATCGCGACCGCCGGCTCGGACGCCAAGCTGGAGCGGGCGCGGGCGCTCGGCGCGGACG
AGGTCGTGAATTACGAGGCGAAGGACTTCGTCCAGGAGGTGAAGCGGCTCACGCAGCGCCGCGGGGTGGACGTGGTGTTC
GAGCACGTGGGCAGGAAGACCTGGGAGGGCTCGATCCTGGCCTGCGCGCCCGGCGGCCGCATCGTCACGGTCGGCGCGAC
CACCGGCTACGATCCCCCGACCGACCTGCGGCACGTGTTCTACCGCCAGCTCTCCATCCTCGGCTCGACCATGGGCACCT
CCGGCGAGCTGCTCGAGGTGCTCCGCTTCGTGGGCGAGGGGAGGCTGCGGCCGGTGGTGGACCGGGTGCTCCCGCTCGCC
CGGGTGCGCGAGGCGCAGGCGCTGCTTGCCGATCGCGCGCAGTTCGGGAAGATCGTCCTCACGCCCTGA

Upstream 100 bases:

>100_bases
GGCGGACTTCCAGACGAGCGGCACCGCCGCCGTGATCTGCGCGGCTCGCTGGTTCGACCAGAATCCGACGTACCAGTCCT
TCTAGCGAACGGGAGCGCAC

Downstream 100 bases:

>100_bases
CGCAGGAGCCCATCATGAAGACGCGCAACGCCCTCTCCGCCGCCGCTCTCGCTGCCACCGCGCTCCTCGTGGCCGGCTGC
GGCAACGACACCTGCCCGAC

Product: alcohol dehydrogenase zinc-binding domain-containing protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKAVLIRGHGGPERVEVGELPLPEPGPGEVRVRVRAAALNHLDLWVRKGWSGLMLSFPHVLGSDVAGVVDAVGPGVDEPK
VGAEVVLAPGLSCGRCVRCRSGEENLCRGYAILGEHVSGGQAEAVVVPARNALPLPPNLSFEEAAAVPLTFLTAWHALVA
RARVRPGETVLVHAAGSGVGVAAVQIAKLHGARVIATAGSDAKLERARALGADEVVNYEAKDFVQEVKRLTQRRGVDVVF
EHVGRKTWEGSILACAPGGRIVTVGATTGYDPPTDLRHVFYRQLSILGSTMGTSGELLEVLRFVGEGRLRPVVDRVLPLA
RVREAQALLADRAQFGKIVLTP

Sequences:

>Translated_342_residues
MKAVLIRGHGGPERVEVGELPLPEPGPGEVRVRVRAAALNHLDLWVRKGWSGLMLSFPHVLGSDVAGVVDAVGPGVDEPK
VGAEVVLAPGLSCGRCVRCRSGEENLCRGYAILGEHVSGGQAEAVVVPARNALPLPPNLSFEEAAAVPLTFLTAWHALVA
RARVRPGETVLVHAAGSGVGVAAVQIAKLHGARVIATAGSDAKLERARALGADEVVNYEAKDFVQEVKRLTQRRGVDVVF
EHVGRKTWEGSILACAPGGRIVTVGATTGYDPPTDLRHVFYRQLSILGSTMGTSGELLEVLRFVGEGRLRPVVDRVLPLA
RVREAQALLADRAQFGKIVLTP
>Mature_342_residues
MKAVLIRGHGGPERVEVGELPLPEPGPGEVRVRVRAAALNHLDLWVRKGWSGLMLSFPHVLGSDVAGVVDAVGPGVDEPK
VGAEVVLAPGLSCGRCVRCRSGEENLCRGYAILGEHVSGGQAEAVVVPARNALPLPPNLSFEEAAAVPLTFLTAWHALVA
RARVRPGETVLVHAAGSGVGVAAVQIAKLHGARVIATAGSDAKLERARALGADEVVNYEAKDFVQEVKRLTQRRGVDVVF
EHVGRKTWEGSILACAPGGRIVTVGATTGYDPPTDLRHVFYRQLSILGSTMGTSGELLEVLRFVGEGRLRPVVDRVLPLA
RVREAQALLADRAQFGKIVLTP

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=359, Percent_Identity=33.1476323119777, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI22538444, Length=359, Percent_Identity=33.1476323119777, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI194239674, Length=351, Percent_Identity=28.4900284900285, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI13236495, Length=351, Percent_Identity=28.4900284900285, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI18379349, Length=369, Percent_Identity=28.9972899728997, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI24308257, Length=370, Percent_Identity=27.8378378378378, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194239676, Length=342, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI47519420, Length=340, Percent_Identity=27.9411764705882, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI41872631, Length=246, Percent_Identity=30.8943089430894, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4501939, Length=357, Percent_Identity=26.3305322128852, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI156523966, Length=357, Percent_Identity=26.3305322128852, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI34577061, Length=255, Percent_Identity=31.3725490196078, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4501933, Length=350, Percent_Identity=27.7142857142857, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI71565152, Length=363, Percent_Identity=26.1707988980716, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI4501929, Length=355, Percent_Identity=27.6056338028169, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI197927207, Length=193, Percent_Identity=29.5336787564767, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI71565154, Length=283, Percent_Identity=28.9752650176678, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI28557745, Length=261, Percent_Identity=30.2681992337165, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI156627571, Length=325, Percent_Identity=28, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI71743840, Length=374, Percent_Identity=25.4010695187166, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI262073058, Length=374, Percent_Identity=25.4010695187166, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI226056130, Length=182, Percent_Identity=31.8681318681319, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI226059159, Length=169, Percent_Identity=31.9526627218935, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI226059133, Length=169, Percent_Identity=31.9526627218935, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790485, Length=347, Percent_Identity=31.1239193083574, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1787863, Length=347, Percent_Identity=28.2420749279539, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI87081918, Length=326, Percent_Identity=29.7546012269939, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790045, Length=284, Percent_Identity=31.3380281690141, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI226510992, Length=294, Percent_Identity=28.2312925170068, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788407, Length=343, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788075, Length=354, Percent_Identity=26.271186440678, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788073, Length=213, Percent_Identity=30.5164319248826, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082401, Length=338, Percent_Identity=28.1065088757396, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082125, Length=359, Percent_Identity=26.4623955431755, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786518, Length=325, Percent_Identity=26.7692307692308, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786552, Length=254, Percent_Identity=29.5275590551181, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789651, Length=292, Percent_Identity=26.3698630136986, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI226510941, Length=121, Percent_Identity=31.404958677686, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17507255, Length=347, Percent_Identity=26.8011527377522, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17562876, Length=266, Percent_Identity=34.2105263157895, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17562878, Length=216, Percent_Identity=34.2592592592593, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17562584, Length=307, Percent_Identity=26.3843648208469, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988145, Length=340, Percent_Identity=25.5882352941176, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562582, Length=311, Percent_Identity=27.3311897106109, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI212642053, Length=248, Percent_Identity=26.6129032258064, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71987554, Length=387, Percent_Identity=24.2894056847545, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319621, Length=300, Percent_Identity=30.6666666666667, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6319520, Length=350, Percent_Identity=26.8571428571429, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323961, Length=327, Percent_Identity=28.1345565749235, Blast_Score=105, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6324486, Length=339, Percent_Identity=26.8436578171091, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=331, Percent_Identity=25.6797583081571, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320033, Length=295, Percent_Identity=27.7966101694915, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6319955, Length=342, Percent_Identity=27.1929824561404, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322619, Length=342, Percent_Identity=27.1929824561404, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319258, Length=317, Percent_Identity=28.0757097791798, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323099, Length=265, Percent_Identity=27.9245283018868, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221330659, Length=233, Percent_Identity=30.4721030042918, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19920632, Length=233, Percent_Identity=30.4721030042918, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24581345, Length=225, Percent_Identity=29.7777777777778, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137530, Length=228, Percent_Identity=28.5087719298246, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17737895, Length=258, Percent_Identity=25.968992248062, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 36212; Mature: 36212

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVLIRGHGGPERVEVGELPLPEPGPGEVRVRVRAAALNHLDLWVRKGWSGLMLSFPHV
CCEEEEECCCCCCCEEECCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHH
LGSDVAGVVDAVGPGVDEPKVGAEVVLAPGLSCGRCVRCRSGEENLCRGYAILGEHVSGG
HCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHCCCCCCCC
QAEAVVVPARNALPLPPNLSFEEAAAVPLTFLTAWHALVARARVRPGETVLVHAAGSGVG
CCCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
VAAVQIAKLHGARVIATAGSDAKLERARALGADEVVNYEAKDFVQEVKRLTQRRGVDVVF
HHHHHHHHHCCCEEEEECCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
EHVGRKTWEGSILACAPGGRIVTVGATTGYDPPTDLRHVFYRQLSILGSTMGTSGELLEV
HHCCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
LRFVGEGRLRPVVDRVLPLARVREAQALLADRAQFGKIVLTP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure
MKAVLIRGHGGPERVEVGELPLPEPGPGEVRVRVRAAALNHLDLWVRKGWSGLMLSFPHV
CCEEEEECCCCCCCEEECCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHH
LGSDVAGVVDAVGPGVDEPKVGAEVVLAPGLSCGRCVRCRSGEENLCRGYAILGEHVSGG
HCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHCCCCCCCC
QAEAVVVPARNALPLPPNLSFEEAAAVPLTFLTAWHALVARARVRPGETVLVHAAGSGVG
CCCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
VAAVQIAKLHGARVIATAGSDAKLERARALGADEVVNYEAKDFVQEVKRLTQRRGVDVVF
HHHHHHHHHCCCEEEEECCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
EHVGRKTWEGSILACAPGGRIVTVGATTGYDPPTDLRHVFYRQLSILGSTMGTSGELLEV
HHCCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
LRFVGEGRLRPVVDRVLPLARVREAQALLADRAQFGKIVLTP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]