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The map label for this gene is rffA [H]

Identifier: 220918071

GI number: 220918071

Start: 3290629

End: 3291750

Strand: Reverse

Name: rffA [H]

Synonym: A2cp1_2972

Alternate gene names: 220918071

Gene position: 3291750-3290629 (Counterclockwise)

Preceding gene: 220918072

Following gene: 220918066

Centisome position: 65.45

GC content: 71.57

Gene sequence:

>1122_bases
GTGATTCCGTTCAACAAGCCGGTGTGGCTGGGCACCGAGATCCTGCGCATCACCGAGGCCATCACCACGCACGGGCACGT
CGCGGGCGGCGGGCCCTTCGCGCGCGCGTGCGAGGAGAGCCTCGCGCGGATGCTCGGCCAGCCGGCGCTCCTGGTCACCT
CGTGCACGCACGCGCTCGAGATGGCGGCGCTGCTGCTCGACGTGAAGGAGGGGGACGAGTTCATCGTCCCCTCCTACACG
TTCGTCTCCAGCGCGAACGCGTTCGTGCTGCGCGGCGCGCGCCCGGTGTTCGCCGACGTCGATCCGAACGGCAACCTGGA
TCCGCGGGAGGTGACGCGCCTCCGGACGCCGCGCACCCGCGCGGTCGTGGCGGTCCACTACGCCGGCAGCTCGTGCGACA
TGACCGACCTGCTGGACCGCTGCGGCCGGATCCCGCTGGTGGAGGACGCGGCCCAGGCGCTCGGGGCCACCTTCGACGGC
AAGCCGCTCGGCACGTTCGGCGTCGCCGGGGCGATCAGCTTCCACGAGACGAAGAACGTGGGCTGCGGGGAGGGCGGGGC
GCTGACCGTGGCCGATCCCGCGCTGCTGGAGCGGGCCGAGTACCTGCGCGACAAGGGCACCAACCGGCGTCGATTCCTGA
CCGGCCTCGTGGACAAGTACACCTGGGTGGACGTGGGCTCCAGCTACGCGCTCTCGGACCTGAACGCCGCCTACCTGTCG
GCGCAGCTCGACGCCTTCGAGCGGATCCAGGCGCGACGCGCGACGCTCCACGATCGGTACGTCCGCGAGCTGGAGCGTGC
GGTGGAGCGAAAGGGCGGGTACCTGGTGCGCCCGGACCCGCGGAACCGCCCGAACCACCACCTGCTGGGGCTCGTGCTCC
GCGCCCGCGAGCAGCGCGACCGGTTCATCGCGCACATGAAGGCGCACGGGATCACCACGCCCTTCCACTACGTCGCGCTC
CACCTCTCGCCCATGGGGAAGCGCTTCCACGGCGGCGAGGCGCTGCCCGGCGCGGAGCGGCTCACCGACTGCCTCGTCCG
CCTGCCGCTGTTCTTCAACATGACGGACGCGGAGCAGGACGAGGTCATCGCGCGGACGCTGGAGTTCGTCGACGCGCTCT
GA

Upstream 100 bases:

>100_bases
CCCTGCCGAAGACCGGGACGGACGGTACTTGAGGGGCCCCTGCATTCCGCCGAGAATGCCGCCCTTGGTCGCCGTGGGTC
GGCGCCGGGGGGGAAGGACA

Downstream 100 bases:

>100_bases
TCGCCCGGGAACCGCGGTGCTCCCGGCCCGGCCCGACGCGTCCGGCGGACGACGTGCGGGCGCTCACGGCGGCGGTCGAG
CCGCGACCGGCACGGACCGG

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MIPFNKPVWLGTEILRITEAITTHGHVAGGGPFARACEESLARMLGQPALLVTSCTHALEMAALLLDVKEGDEFIVPSYT
FVSSANAFVLRGARPVFADVDPNGNLDPREVTRLRTPRTRAVVAVHYAGSSCDMTDLLDRCGRIPLVEDAAQALGATFDG
KPLGTFGVAGAISFHETKNVGCGEGGALTVADPALLERAEYLRDKGTNRRRFLTGLVDKYTWVDVGSSYALSDLNAAYLS
AQLDAFERIQARRATLHDRYVRELERAVERKGGYLVRPDPRNRPNHHLLGLVLRAREQRDRFIAHMKAHGITTPFHYVAL
HLSPMGKRFHGGEALPGAERLTDCLVRLPLFFNMTDAEQDEVIARTLEFVDAL

Sequences:

>Translated_373_residues
MIPFNKPVWLGTEILRITEAITTHGHVAGGGPFARACEESLARMLGQPALLVTSCTHALEMAALLLDVKEGDEFIVPSYT
FVSSANAFVLRGARPVFADVDPNGNLDPREVTRLRTPRTRAVVAVHYAGSSCDMTDLLDRCGRIPLVEDAAQALGATFDG
KPLGTFGVAGAISFHETKNVGCGEGGALTVADPALLERAEYLRDKGTNRRRFLTGLVDKYTWVDVGSSYALSDLNAAYLS
AQLDAFERIQARRATLHDRYVRELERAVERKGGYLVRPDPRNRPNHHLLGLVLRAREQRDRFIAHMKAHGITTPFHYVAL
HLSPMGKRFHGGEALPGAERLTDCLVRLPLFFNMTDAEQDEVIARTLEFVDAL
>Mature_373_residues
MIPFNKPVWLGTEILRITEAITTHGHVAGGGPFARACEESLARMLGQPALLVTSCTHALEMAALLLDVKEGDEFIVPSYT
FVSSANAFVLRGARPVFADVDPNGNLDPREVTRLRTPRTRAVVAVHYAGSSCDMTDLLDRCGRIPLVEDAAQALGATFDG
KPLGTFGVAGAISFHETKNVGCGEGGALTVADPALLERAEYLRDKGTNRRRFLTGLVDKYTWVDVGSSYALSDLNAAYLS
AQLDAFERIQARRATLHDRYVRELERAVERKGGYLVRPDPRNRPNHHLLGLVLRAREQRDRFIAHMKAHGITTPFHYVAL
HLSPMGKRFHGGEALPGAERLTDCLVRLPLFFNMTDAEQDEVIARTLEFVDAL

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=379, Percent_Identity=45.910290237467, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI145693159, Length=380, Percent_Identity=30.5263157894737, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 41056; Mature: 41056

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFNKPVWLGTEILRITEAITTHGHVAGGGPFARACEESLARMLGQPALLVTSCTHALE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MAALLLDVKEGDEFIVPSYTFVSSANAFVLRGARPVFADVDPNGNLDPREVTRLRTPRTR
HHHHHHHHCCCCCEECCCCHHHCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHCCCCCE
AVVAVHYAGSSCDMTDLLDRCGRIPLVEDAAQALGATFDGKPLGTFGVAGAISFHETKNV
EEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCEEEECCCCCC
GCGEGGALTVADPALLERAEYLRDKGTNRRRFLTGLVDKYTWVDVGSSYALSDLNAAYLS
CCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHH
AQLDAFERIQARRATLHDRYVRELERAVERKGGYLVRPDPRNRPNHHLLGLVLRAREQRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHH
RFIAHMKAHGITTPFHYVALHLSPMGKRFHGGEALPGAERLTDCLVRLPLFFNMTDAEQD
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHH
EVIARTLEFVDAL
HHHHHHHHHHHCC
>Mature Secondary Structure
MIPFNKPVWLGTEILRITEAITTHGHVAGGGPFARACEESLARMLGQPALLVTSCTHALE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MAALLLDVKEGDEFIVPSYTFVSSANAFVLRGARPVFADVDPNGNLDPREVTRLRTPRTR
HHHHHHHHCCCCCEECCCCHHHCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHCCCCCE
AVVAVHYAGSSCDMTDLLDRCGRIPLVEDAAQALGATFDGKPLGTFGVAGAISFHETKNV
EEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCEEEECCCCCC
GCGEGGALTVADPALLERAEYLRDKGTNRRRFLTGLVDKYTWVDVGSSYALSDLNAAYLS
CCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHH
AQLDAFERIQARRATLHDRYVRELERAVERKGGYLVRPDPRNRPNHHLLGLVLRAREQRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHH
RFIAHMKAHGITTPFHYVALHLSPMGKRFHGGEALPGAERLTDCLVRLPLFFNMTDAEQD
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHH
EVIARTLEFVDAL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]