Click here to switch to the map view.

The map label for this gene is lcfB [H]

Identifier: 220918057

GI number: 220918057

Start: 3270192

End: 3271763

Strand: Direct

Name: lcfB [H]

Synonym: A2cp1_2958

Alternate gene names: 220918057

Gene position: 3270192-3271763 (Clockwise)

Preceding gene: 220918053

Following gene: 220918058

Centisome position: 65.02

GC content: 74.87

Gene sequence:

>1572_bases
GTGCGATCTCCGTTCCTACACGACTGGCTGTTCGCGCACGCCGAGCGGCGCCCCGAGGCGCCGGCGGTGGCGACGCCCTC
CATGCGCGTGACGTACGGCGAGCTCGCCGGGCGCGTGCGCGCGCTCGCGGGCCACCTCGCGGCCCGGGGCGTACGTCCCG
GCGACCGGGTCGTGCTCGCGCTGCCGAACCTTCCGGCGACCGTCGCGGCGGGGCTCGCCGTGAACGCGCTCGGCGCCACC
ACCGTCGAGGTGAACCGGGAGTGGTCGCCGGAGATCCTCGGGCGCATCCTCGCGCAGACCCGGACGCGCCACGCGCTGGT
CTGGGGTCGCGACGCACGGACGTGGGGTGGGCTCTGCCGGAGCCACCCGCTCGAGCGACTCTGGGTCGTGCACCCCGGCG
CCCCGCCGGCGGCGCTCCTCGACGCGCTCGGCGGCACCCCTGCGACCTGGGTGATCGAGGACGGCCGGGTGGATCCGGCC
GAGGGTGCGGCGCCGCCCCCGCCCTCCCCGGCGCTCTCGCCCGACCAGCCCGCGCTCGTCCTCTACACCTCCGGCAGCAC
CGGCCAGCCGCGCGGCGTGGTGCAGACGTTCCGGAACGTGGACGCGAACTCGCGCTCCATCGTCCAGTACCTCGAGCTCG
GCGAGTCCGACCGCGCGCTGCTGATCCTCCCGCTCTACTACTGCTACGGGCGCAGCGTGCTGCAGACGCACCTGCTGGCC
GGCGGCTCAATGTTCCTGGACGGCCGCTTCGCGTTCCCGCGCGTGGTCCTCGAGGCCATGACCGGCGAGGGCTGCACCGG
CTTCGCCGGCGTCCCGCTCACGTTCGAGATCATCCGCCGCCAGGTGGACGTCGCGTCCATGCGCTTCCCCACCCTGCGCT
ACCTGACGCAGGCCGGCGGCGCGATGGCGCCGGACACGGTCGCCTGGGTGCGCAACGCGTTCCAGCCCGCGCGGCTGTTC
GTCATGTACGGGCAGACCGAGGCGACCGCGCGGCTCGCCTACCTGCCGCCGGAGCGTGGCGAGGAGAAGCTCGGCTCGAT
GGGCGTCGCCATCCCGGGCGTCGAGCTCCGGGTGGTGGACGACGGCGGCCGGGAGCTGGCCACCGGCGAGACCGGCCACC
TGGTGGCGCGCGGCGACAACGTGACGCTCGGTTACCTGGACGAGCCCGAGGAGACCGCCGCCATCCTGCACGACGGGTGG
CTGTGGACGGGCGACCTCGCCTCGCGCGACGCGGACGGCTTCTTCTTCCACCGCGGCCGGTCGAAGGAGATCCTCAAGGT
CGGCGGGCACCGCGTGAGCCCGATCGAGATCGAGCACGCGGTGGCCCGCCACCCCGACGTCGCCGAGGCGGCGGTCGTCG
GGGTGCAGGACGCGCTGATGGGCGAGCTGCCGGTGGCGTTCGTGGTGCCGCGCCCCGGCGCGTCGCCGACCGAGGACGAC
CTGCGCCGCTTCTGCCGCGAGCACCTGCCGGCGTACCAGGTCCCGGTGCGCTTCACGTTCGTGGACGCGCTGCCGCGGAA
CGAGTCGGGGAAGCTGCTGCGTGCGGAGCTGGCGCGGAGGACGGGGCCGTGA

Upstream 100 bases:

>100_bases
TTCCATGAGCACGGGACGCCGCGCGGCATTGCGCGCGCGGCCGCGTGGGGGTACCGATGGTGGCGCGGTGCGGGTCTGGC
GACCAGGGGTGGGCGCTGCC

Downstream 100 bases:

>100_bases
GGCGGATCGCGATGGTCGGGCACGGAGACTACTCCGCCGCGCTCGAGCTCATCGCGCGCGGCGAGCCCGAGCCGTACTTC
GGCATGGCCTACACCCTCCG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MRSPFLHDWLFAHAERRPEAPAVATPSMRVTYGELAGRVRALAGHLAARGVRPGDRVVLALPNLPATVAAGLAVNALGAT
TVEVNREWSPEILGRILAQTRTRHALVWGRDARTWGGLCRSHPLERLWVVHPGAPPAALLDALGGTPATWVIEDGRVDPA
EGAAPPPPSPALSPDQPALVLYTSGSTGQPRGVVQTFRNVDANSRSIVQYLELGESDRALLILPLYYCYGRSVLQTHLLA
GGSMFLDGRFAFPRVVLEAMTGEGCTGFAGVPLTFEIIRRQVDVASMRFPTLRYLTQAGGAMAPDTVAWVRNAFQPARLF
VMYGQTEATARLAYLPPERGEEKLGSMGVAIPGVELRVVDDGGRELATGETGHLVARGDNVTLGYLDEPEETAAILHDGW
LWTGDLASRDADGFFFHRGRSKEILKVGGHRVSPIEIEHAVARHPDVAEAAVVGVQDALMGELPVAFVVPRPGASPTEDD
LRRFCREHLPAYQVPVRFTFVDALPRNESGKLLRAELARRTGP

Sequences:

>Translated_523_residues
MRSPFLHDWLFAHAERRPEAPAVATPSMRVTYGELAGRVRALAGHLAARGVRPGDRVVLALPNLPATVAAGLAVNALGAT
TVEVNREWSPEILGRILAQTRTRHALVWGRDARTWGGLCRSHPLERLWVVHPGAPPAALLDALGGTPATWVIEDGRVDPA
EGAAPPPPSPALSPDQPALVLYTSGSTGQPRGVVQTFRNVDANSRSIVQYLELGESDRALLILPLYYCYGRSVLQTHLLA
GGSMFLDGRFAFPRVVLEAMTGEGCTGFAGVPLTFEIIRRQVDVASMRFPTLRYLTQAGGAMAPDTVAWVRNAFQPARLF
VMYGQTEATARLAYLPPERGEEKLGSMGVAIPGVELRVVDDGGRELATGETGHLVARGDNVTLGYLDEPEETAAILHDGW
LWTGDLASRDADGFFFHRGRSKEILKVGGHRVSPIEIEHAVARHPDVAEAAVVGVQDALMGELPVAFVVPRPGASPTEDD
LRRFCREHLPAYQVPVRFTFVDALPRNESGKLLRAELARRTGP
>Mature_523_residues
MRSPFLHDWLFAHAERRPEAPAVATPSMRVTYGELAGRVRALAGHLAARGVRPGDRVVLALPNLPATVAAGLAVNALGAT
TVEVNREWSPEILGRILAQTRTRHALVWGRDARTWGGLCRSHPLERLWVVHPGAPPAALLDALGGTPATWVIEDGRVDPA
EGAAPPPPSPALSPDQPALVLYTSGSTGQPRGVVQTFRNVDANSRSIVQYLELGESDRALLILPLYYCYGRSVLQTHLLA
GGSMFLDGRFAFPRVVLEAMTGEGCTGFAGVPLTFEIIRRQVDVASMRFPTLRYLTQAGGAMAPDTVAWVRNAFQPARLF
VMYGQTEATARLAYLPPERGEEKLGSMGVAIPGVELRVVDDGGRELATGETGHLVARGDNVTLGYLDEPEETAAILHDGW
LWTGDLASRDADGFFFHRGRSKEILKVGGHRVSPIEIEHAVARHPDVAEAAVVGVQDALMGELPVAFVVPRPGASPTEDD
LRRFCREHLPAYQVPVRFTFVDALPRNESGKLLRAELARRTGP

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=546, Percent_Identity=29.8534798534799, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI38505220, Length=546, Percent_Identity=28.021978021978, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI187761345, Length=393, Percent_Identity=29.0076335877863, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI187761343, Length=393, Percent_Identity=29.0076335877863, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI42544132, Length=359, Percent_Identity=28.9693593314763, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI157311624, Length=353, Percent_Identity=28.6118980169972, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI157311622, Length=353, Percent_Identity=28.6118980169972, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI115511026, Length=367, Percent_Identity=28.3378746594005, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI58082049, Length=352, Percent_Identity=28.6931818181818, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI122937307, Length=250, Percent_Identity=30.8, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI28416953, Length=546, Percent_Identity=24.3589743589744, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI8923896, Length=537, Percent_Identity=25.3258845437616, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI13375727, Length=382, Percent_Identity=27.4869109947644, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI253970489, Length=362, Percent_Identity=26.7955801104972, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI57165412, Length=363, Percent_Identity=28.099173553719, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI57165410, Length=366, Percent_Identity=28.6885245901639, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI40807491, Length=352, Percent_Identity=26.7045454545455, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI27477105, Length=373, Percent_Identity=25.7372654155496, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=30.4267161410019, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI145693145, Length=510, Percent_Identity=29.0196078431373, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI221142682, Length=495, Percent_Identity=26.6666666666667, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1790505, Length=516, Percent_Identity=26.7441860465116, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1786810, Length=533, Percent_Identity=27.0168855534709, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789201, Length=352, Percent_Identity=30.1136363636364, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1788595, Length=514, Percent_Identity=24.7081712062257, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786529, Length=557, Percent_Identity=25.4937163375224, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786801, Length=536, Percent_Identity=26.6791044776119, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=523, Percent_Identity=29.6367112810707, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17538037, Length=520, Percent_Identity=27.8846153846154, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17559526, Length=522, Percent_Identity=26.4367816091954, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17560308, Length=567, Percent_Identity=28.2186948853616, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17558820, Length=505, Percent_Identity=25.7425742574257, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71996755, Length=521, Percent_Identity=25.1439539347409, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=500, Percent_Identity=26, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17560140, Length=528, Percent_Identity=25, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71994703, Length=531, Percent_Identity=25.4237288135593, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=531, Percent_Identity=25.4237288135593, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=531, Percent_Identity=25.4237288135593, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=540, Percent_Identity=24.0740740740741, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71985884, Length=374, Percent_Identity=25.668449197861, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983001, Length=531, Percent_Identity=25.9887005649718, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71982997, Length=531, Percent_Identity=25.9887005649718, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25141359, Length=360, Percent_Identity=22.7777777777778, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=346, Percent_Identity=30.9248554913295, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323182, Length=561, Percent_Identity=26.7379679144385, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319264, Length=559, Percent_Identity=26.1180679785331, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24581924, Length=526, Percent_Identity=26.8060836501901, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18859661, Length=532, Percent_Identity=27.2556390977444, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI21355181, Length=481, Percent_Identity=27.8586278586279, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21355225, Length=544, Percent_Identity=26.2867647058824, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21356947, Length=348, Percent_Identity=26.1494252873563, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648260, Length=353, Percent_Identity=25.7790368271955, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922652, Length=365, Percent_Identity=28.2191780821918, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI62472339, Length=548, Percent_Identity=25.5474452554745, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20130357, Length=452, Percent_Identity=24.5575221238938, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24667955, Length=527, Percent_Identity=25.426944971537, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356441, Length=512, Percent_Identity=24.4140625, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653035, Length=226, Percent_Identity=30.9734513274336, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281365686, Length=328, Percent_Identity=26.219512195122, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648253, Length=522, Percent_Identity=23.7547892720307, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667959, Length=369, Percent_Identity=26.8292682926829, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648255, Length=522, Percent_Identity=23.9463601532567, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI161076582, Length=239, Percent_Identity=29.7071129707113, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24656500, Length=388, Percent_Identity=27.5773195876289, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358303, Length=152, Percent_Identity=31.5789473684211, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17933690, Length=338, Percent_Identity=24.8520710059172, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648257, Length=361, Percent_Identity=24.3767313019391, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56569; Mature: 56569

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSPFLHDWLFAHAERRPEAPAVATPSMRVTYGELAGRVRALAGHLAARGVRPGDRVVLA
CCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LPNLPATVAAGLAVNALGATTVEVNREWSPEILGRILAQTRTRHALVWGRDARTWGGLCR
CCCCCHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
SHPLERLWVVHPGAPPAALLDALGGTPATWVIEDGRVDPAEGAAPPPPSPALSPDQPALV
CCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEE
LYTSGSTGQPRGVVQTFRNVDANSRSIVQYLELGESDRALLILPLYYCYGRSVLQTHLLA
EEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHCHHHHHHHHHC
GGSMFLDGRFAFPRVVLEAMTGEGCTGFAGVPLTFEIIRRQVDVASMRFPTLRYLTQAGG
CCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCC
AMAPDTVAWVRNAFQPARLFVMYGQTEATARLAYLPPERGEEKLGSMGVAIPGVELRVVD
CCCCHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCHHHHCCCCEEECCEEEEEEE
DGGRELATGETGHLVARGDNVTLGYLDEPEETAAILHDGWLWTGDLASRDADGFFFHRGR
CCCCEECCCCCCEEEEECCCEEEEECCCCHHHHHEEECCCEEECCCCCCCCCCEEEECCC
SKEILKVGGHRVSPIEIEHAVARHPDVAEAAVVGVQDALMGELPVAFVVPRPGASPTEDD
CCHHHHHCCCCCCCEEHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
LRRFCREHLPAYQVPVRFTFVDALPRNESGKLLRAELARRTGP
HHHHHHHCCCCEECCEEEEEEECCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MRSPFLHDWLFAHAERRPEAPAVATPSMRVTYGELAGRVRALAGHLAARGVRPGDRVVLA
CCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LPNLPATVAAGLAVNALGATTVEVNREWSPEILGRILAQTRTRHALVWGRDARTWGGLCR
CCCCCHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
SHPLERLWVVHPGAPPAALLDALGGTPATWVIEDGRVDPAEGAAPPPPSPALSPDQPALV
CCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEE
LYTSGSTGQPRGVVQTFRNVDANSRSIVQYLELGESDRALLILPLYYCYGRSVLQTHLLA
EEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHCHHHHHHHHHC
GGSMFLDGRFAFPRVVLEAMTGEGCTGFAGVPLTFEIIRRQVDVASMRFPTLRYLTQAGG
CCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCC
AMAPDTVAWVRNAFQPARLFVMYGQTEATARLAYLPPERGEEKLGSMGVAIPGVELRVVD
CCCCHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCHHHHCCCCEEECCEEEEEEE
DGGRELATGETGHLVARGDNVTLGYLDEPEETAAILHDGWLWTGDLASRDADGFFFHRGR
CCCCEECCCCCCEEEEECCCEEEEECCCCHHHHHEEECCCEEECCCCCCCCCCEEEECCC
SKEILKVGGHRVSPIEIEHAVARHPDVAEAAVVGVQDALMGELPVAFVVPRPGASPTEDD
CCHHHHHCCCCCCCEEHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
LRRFCREHLPAYQVPVRFTFVDALPRNESGKLLRAELARRTGP
HHHHHHHCCCCEECCEEEEEEECCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]