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The map label for this gene is asnS [H]

Identifier: 220918011

GI number: 220918011

Start: 3225744

End: 3227048

Strand: Direct

Name: asnS [H]

Synonym: A2cp1_2912

Alternate gene names: 220918011

Gene position: 3225744-3227048 (Clockwise)

Preceding gene: 220918008

Following gene: 220918038

Centisome position: 64.14

GC content: 68.12

Gene sequence:

>1305_bases
ATGTACCCGCCCGTCCACGTCGCCGACATCGCCCAGCACGAGGGGAAGGAGATCACGCTCCACGGCTGGCTCCACAACCG
CCGCTCGTCGGGCAAGCTCCACTTCCTCCAGCTGCGCGACGGCACCGGCACGATCCAGTGCGTGGTGTTCAAGGGGAACG
TGACGCCGGAGGTGTTCCAGGCCTCGGACCACCTCCCGCAGGAGACCAGCCTCACGGTGACGGGCCTCGTGAAGAAGGAC
GAGCGCTCGCCCATCGGCTTCGAGCTGGACGTGCGCGACCTGCAGGTGGTGTCGCGCCCGGCCCAGGAGTTCCCCATCGG
GCACAAGGAGCACGGGGTCGCGTTCCTGATGGAGCAGCGGCACCTGTGGCTCCGCTCGCAGCGGCAGCAGGTGATCCTGC
GCGTCCGCCACACCGTCGAGAAGGCGATCCGCGACTTCTTCGACCAGCGCGGCTTCACGCTGGTGGACGCGCCCATCTTC
ACGCCGTCCGCCTGCGAGGGCACCTCCACGCTGTTCGAGGTCCCCTACTTCGACCTGGGCAAGGCCTACCTGACGCAGTC
CGGCCAGCTCTACATGGAGGCCGCGGCCATGGCGCTCGGGAAGGTCTACTGCTTCGGCCCGACCTTCCGCGCCGAGAAGT
CCAAGACGCGCCGCCACCTCACCGAGTTCTGGATGGTCGAGCCGGAGGTCGCGTACATGGACCTCGACGGCGACATGGAG
CTGATGGAGCAGTTCGTCTCCTACGTGGTCCAGACCGCGCTGGAGAAGCACCGCGTCGAGCTCGCCACGGTGCTGGAGCG
CGACGTCTCGAAGCTCGAGAACGTGAAGGCGCCGTTCCCGCGCATCACCTATACCGAGGCGGTGGAGGTCATCCAGAAGG
CCGGCCACCCGATGAAGTGGGGCGACGACATCGGCGGCGACGAGGAGACGGTGGTCGCGAGGCAGTTCGACCGTCCGGTG
ATGGTGCACCGCTACCCCGCCGAGATGAAGGCGTTCTACTTCAAGAAGGACCCCGCCGATCCCAAGGTCGCGCTCGGCTG
CGACGTGCTCGCGCCGGAGGGCTATGGCGAGATCATCGGCGGCGGCCAGCGCGAGGACGACCTCGCCACCCTCGAGGCCG
CCATCGATCACCACCAGCTGCCGAAGCAGGCGTTCGAGTGGTACCTCGACCTGCGCCGCTACGGCACCGTCCCGCACGCC
GGCTTCGGCATGGGGGTGGAGCGCTGCGTGGCCTGGATCTGCGGCCTCCACCACGTCCGCGAGACCATCCCGTTCGCGCG
CATGATGGAGAGGATCACGCCGTGA

Upstream 100 bases:

>100_bases
GACATAGGGGTGCGGATTCTAGCGGACGTCGCGCGTTTCGTCTGCTCAGGTATATTGCCCGCGAAACCACGTCCACCGCC
CATCCAGCACGGGGTCCCGC

Downstream 100 bases:

>100_bases
GTGATCTGACGGGGCTGCCGCCCCGCCCCATGGAGCGGAGCTCCATGGGGCCCCACCCCGCGCGCGGGGCCCGTGACCTC
GCGCGCCCGCTGACGCGGGT

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MYPPVHVADIAQHEGKEITLHGWLHNRRSSGKLHFLQLRDGTGTIQCVVFKGNVTPEVFQASDHLPQETSLTVTGLVKKD
ERSPIGFELDVRDLQVVSRPAQEFPIGHKEHGVAFLMEQRHLWLRSQRQQVILRVRHTVEKAIRDFFDQRGFTLVDAPIF
TPSACEGTSTLFEVPYFDLGKAYLTQSGQLYMEAAAMALGKVYCFGPTFRAEKSKTRRHLTEFWMVEPEVAYMDLDGDME
LMEQFVSYVVQTALEKHRVELATVLERDVSKLENVKAPFPRITYTEAVEVIQKAGHPMKWGDDIGGDEETVVARQFDRPV
MVHRYPAEMKAFYFKKDPADPKVALGCDVLAPEGYGEIIGGGQREDDLATLEAAIDHHQLPKQAFEWYLDLRRYGTVPHA
GFGMGVERCVAWICGLHHVRETIPFARMMERITP

Sequences:

>Translated_434_residues
MYPPVHVADIAQHEGKEITLHGWLHNRRSSGKLHFLQLRDGTGTIQCVVFKGNVTPEVFQASDHLPQETSLTVTGLVKKD
ERSPIGFELDVRDLQVVSRPAQEFPIGHKEHGVAFLMEQRHLWLRSQRQQVILRVRHTVEKAIRDFFDQRGFTLVDAPIF
TPSACEGTSTLFEVPYFDLGKAYLTQSGQLYMEAAAMALGKVYCFGPTFRAEKSKTRRHLTEFWMVEPEVAYMDLDGDME
LMEQFVSYVVQTALEKHRVELATVLERDVSKLENVKAPFPRITYTEAVEVIQKAGHPMKWGDDIGGDEETVVARQFDRPV
MVHRYPAEMKAFYFKKDPADPKVALGCDVLAPEGYGEIIGGGQREDDLATLEAAIDHHQLPKQAFEWYLDLRRYGTVPHA
GFGMGVERCVAWICGLHHVRETIPFARMMERITP
>Mature_434_residues
MYPPVHVADIAQHEGKEITLHGWLHNRRSSGKLHFLQLRDGTGTIQCVVFKGNVTPEVFQASDHLPQETSLTVTGLVKKD
ERSPIGFELDVRDLQVVSRPAQEFPIGHKEHGVAFLMEQRHLWLRSQRQQVILRVRHTVEKAIRDFFDQRGFTLVDAPIF
TPSACEGTSTLFEVPYFDLGKAYLTQSGQLYMEAAAMALGKVYCFGPTFRAEKSKTRRHLTEFWMVEPEVAYMDLDGDME
LMEQFVSYVVQTALEKHRVELATVLERDVSKLENVKAPFPRITYTEAVEVIQKAGHPMKWGDDIGGDEETVVARQFDRPV
MVHRYPAEMKAFYFKKDPADPKVALGCDVLAPEGYGEIIGGGQREDDLATLEAAIDHHQLPKQAFEWYLDLRRYGTVPHA
GFGMGVERCVAWICGLHHVRETIPFARMMERITP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4758762, Length=444, Percent_Identity=32.6576576576577, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI226491502, Length=441, Percent_Identity=34.4671201814059, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI45439306, Length=462, Percent_Identity=29.004329004329, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI40789249, Length=254, Percent_Identity=26.7716535433071, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787161, Length=460, Percent_Identity=38.2608695652174, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1788173, Length=247, Percent_Identity=25.9109311740891, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=444, Percent_Identity=34.6846846846847, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI17551876, Length=454, Percent_Identity=27.7533039647577, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17556372, Length=409, Percent_Identity=29.0953545232274, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321807, Length=434, Percent_Identity=35.4838709677419, Blast_Score=260, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6319872, Length=457, Percent_Identity=30.8533916849015, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6323011, Length=472, Percent_Identity=30.7203389830508, Blast_Score=160, Evalue=3e-40,
Organism=Drosophila melanogaster, GI19921528, Length=444, Percent_Identity=32.6576576576577, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI19922416, Length=446, Percent_Identity=32.5112107623318, Blast_Score=214, Evalue=7e-56,
Organism=Drosophila melanogaster, GI17136276, Length=461, Percent_Identity=27.765726681128, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 49395; Mature: 49395

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPPVHVADIAQHEGKEITLHGWLHNRRSSGKLHFLQLRDGTGTIQCVVFKGNVTPEVFQ
CCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCEEEEEEEECCCCHHHHH
ASDHLPQETSLTVTGLVKKDERSPIGFELDVRDLQVVSRPAQEFPIGHKEHGVAFLMEQR
HCCCCCCCCCEEEEEEEECCCCCCCCEEECHHHHHHHHCCHHHCCCCCCCCCHHHHHHHH
HLWLRSQRQQVILRVRHTVEKAIRDFFDQRGFTLVDAPIFTPSACEGTSTLFEVPYFDLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCHHCC
KAYLTQSGQLYMEAAAMALGKVYCFGPTFRAEKSKTRRHLTEFWMVEPEVAYMDLDGDME
HHHHCCCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHEECCCEEEEECCCCHH
LMEQFVSYVVQTALEKHRVELATVLERDVSKLENVKAPFPRITYTEAVEVIQKAGHPMKW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
GDDIGGDEETVVARQFDRPVMVHRYPAEMKAFYFKKDPADPKVALGCDVLAPEGYGEIIG
CCCCCCCCHHHHHHHCCCCEEEEECCHHHHHEEEECCCCCCCEEECCEEECCCCCHHHCC
GGQREDDLATLEAAIDHHQLPKQAFEWYLDLRRYGTVPHAGFGMGVERCVAWICGLHHVR
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
ETIPFARMMERITP
HHCCHHHHHHHHCC
>Mature Secondary Structure
MYPPVHVADIAQHEGKEITLHGWLHNRRSSGKLHFLQLRDGTGTIQCVVFKGNVTPEVFQ
CCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCEEEEEEEECCCCHHHHH
ASDHLPQETSLTVTGLVKKDERSPIGFELDVRDLQVVSRPAQEFPIGHKEHGVAFLMEQR
HCCCCCCCCCEEEEEEEECCCCCCCCEEECHHHHHHHHCCHHHCCCCCCCCCHHHHHHHH
HLWLRSQRQQVILRVRHTVEKAIRDFFDQRGFTLVDAPIFTPSACEGTSTLFEVPYFDLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCHHCC
KAYLTQSGQLYMEAAAMALGKVYCFGPTFRAEKSKTRRHLTEFWMVEPEVAYMDLDGDME
HHHHCCCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHEECCCEEEEECCCCHH
LMEQFVSYVVQTALEKHRVELATVLERDVSKLENVKAPFPRITYTEAVEVIQKAGHPMKW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
GDDIGGDEETVVARQFDRPVMVHRYPAEMKAFYFKKDPADPKVALGCDVLAPEGYGEIIG
CCCCCCCCHHHHHHHCCCCEEEEECCHHHHHEEEECCCCCCCEEECCEEECCCCCHHHCC
GGQREDDLATLEAAIDHHQLPKQAFEWYLDLRRYGTVPHAGFGMGVERCVAWICGLHHVR
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
ETIPFARMMERITP
HHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA