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The map label for this gene is prfB [H]

Identifier: 220918000

GI number: 220918000

Start: 3210093

End: 3211022

Strand: Reverse

Name: prfB [H]

Synonym: A2cp1_2901

Alternate gene names: 220918000

Gene position: 3211022-3210093 (Counterclockwise)

Preceding gene: 220918001

Following gene: 220917995

Centisome position: 63.85

GC content: 71.4

Gene sequence:

>930_bases
GTGACCCAGGCCTGCGCCGCGGTCCGCCGCGCGCTCGACGACGCGCAGGCGCTGTGCGAGCTCGCCGAGGAGGCGCGCGA
CGAGGCCACCCGCGCCGAGGCGGCCGAGGCCGCCGAGGCGGTGGGCCGCCAGCTCGAGGGGCTCGAGCTGCAGAAGATGC
TCTCCGGCCCGCACGACGCGGCCGGCGCCATCGTCGAGGTGAAGAGCGGCGCGGGCGGCGTGGAGGCGATGGACTGGGCC
GCCATGCTGTACCGGATGTACGTGCGCTACTGCGAGCGGCGCGGCTGGGAGGTCGAGCCGGCCGACCTGGTCGCGGGCGA
GGAGGCCGGGATCAGCTCCGCCTCGTTCATCGTCCGCGGCGATCACCCCTACGGCTGGCTGAAGGCCGAGAAGGGCGTGC
ACCGGCTGGTCCGCATCAGCCCGTTCGACCAGAACGCGCGCCGCCAGACCAGCTTCGCGGCGGTGGACGTGACCCCGGAG
ATCGAGGACGACATCGTCATCGACATCAAGGAGGCCGACGTCCGCATCGACACGTACCGGTCGTCGGGCGCCGGCGGCCA
GAAGGTGAACAAGACCGACTCCGCGGTGCGCATGACGCACCTGCCCACCGGCATCGTGGTGGCGGTGCAGAACGAGCGCA
GCCAGCAGAAGAACCGCAGCGTGGCCTGGAAGATCCTGCGCGCCAAGCTCTACGACCTCGAGGAGAAGAAGCGCGCGGCG
GCCCGCGACGCCGCCGAGGCGCTGAAGAAGGACATCGACTTCGGCTCGCAGATCCGCAGCTACGTGCTGCAGCCCTACCA
GATGGTGAAGGACCTGCGCACCGGGGTGGAGACGGGCAAGGTGGACGACGTCCTGGACGGCGACCTCGACCCGTTCATCC
AGCCCTACCTGATGGGCGTGCGGCGCACCGACCGCGCCGTGGACGAGTGA

Upstream 100 bases:

>100_bases
GAGGGTCGCGGAGATCTCGAAGCTCTCCGAGGACCCGGCGTTCTGGACCGACAACGTCAAGGCCCAGGCGCTGCTGAAGG
AGAAGGCCCAGCTCGAGCAG

Downstream 100 bases:

>100_bases
CCCGGGCGGCGCGCCGCTACCGCGGCGTGTAGACGATGGGCTGGCGCGCGAGCGTGAAGCGGTCGATGGCGTCCATCGCC
CAGCCCGGCAGCGCGAGCAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTQACAAVRRALDDAQALCELAEEARDEATRAEAAEAAEAVGRQLEGLELQKMLSGPHDAAGAIVEVKSGAGGVEAMDWA
AMLYRMYVRYCERRGWEVEPADLVAGEEAGISSASFIVRGDHPYGWLKAEKGVHRLVRISPFDQNARRQTSFAAVDVTPE
IEDDIVIDIKEADVRIDTYRSSGAGGQKVNKTDSAVRMTHLPTGIVVAVQNERSQQKNRSVAWKILRAKLYDLEEKKRAA
ARDAAEALKKDIDFGSQIRSYVLQPYQMVKDLRTGVETGKVDDVLDGDLDPFIQPYLMGVRRTDRAVDE

Sequences:

>Translated_309_residues
MTQACAAVRRALDDAQALCELAEEARDEATRAEAAEAAEAVGRQLEGLELQKMLSGPHDAAGAIVEVKSGAGGVEAMDWA
AMLYRMYVRYCERRGWEVEPADLVAGEEAGISSASFIVRGDHPYGWLKAEKGVHRLVRISPFDQNARRQTSFAAVDVTPE
IEDDIVIDIKEADVRIDTYRSSGAGGQKVNKTDSAVRMTHLPTGIVVAVQNERSQQKNRSVAWKILRAKLYDLEEKKRAA
ARDAAEALKKDIDFGSQIRSYVLQPYQMVKDLRTGVETGKVDDVLDGDLDPFIQPYLMGVRRTDRAVDE
>Mature_308_residues
TQACAAVRRALDDAQALCELAEEARDEATRAEAAEAAEAVGRQLEGLELQKMLSGPHDAAGAIVEVKSGAGGVEAMDWAA
MLYRMYVRYCERRGWEVEPADLVAGEEAGISSASFIVRGDHPYGWLKAEKGVHRLVRISPFDQNARRQTSFAAVDVTPEI
EDDIVIDIKEADVRIDTYRSSGAGGQKVNKTDSAVRMTHLPTGIVVAVQNERSQQKNRSVAWKILRAKLYDLEEKKRAAA
RDAAEALKKDIDFGSQIRSYVLQPYQMVKDLRTGVETGKVDDVLDGDLDPFIQPYLMGVRRTDRAVDE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=244, Percent_Identity=38.5245901639344, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI34577120, Length=312, Percent_Identity=31.0897435897436, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI166795305, Length=146, Percent_Identity=41.7808219178082, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI2367172, Length=290, Percent_Identity=52.7586206896552, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1787462, Length=245, Percent_Identity=43.6734693877551, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=207, Percent_Identity=35.2657004830918, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=308, Percent_Identity=34.7402597402597, Blast_Score=142, Evalue=6e-35,
Organism=Drosophila melanogaster, GI19921226, Length=246, Percent_Identity=39.4308943089431, Blast_Score=157, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 34111; Mature: 33980

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQACAAVRRALDDAQALCELAEEARDEATRAEAAEAAEAVGRQLEGLELQKMLSGPHDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCC
AGAIVEVKSGAGGVEAMDWAAMLYRMYVRYCERRGWEVEPADLVAGEEAGISSASFIVRG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCEEEEEC
DHPYGWLKAEKGVHRLVRISPFDQNARRQTSFAAVDVTPEIEDDIVIDIKEADVRIDTYR
CCCCCHHHHHHHHHHHEEECCCCCCHHHHCCEEEEECCCCCCCCEEEEEECCCEEEEECC
SSGAGGQKVNKTDSAVRMTHLPTGIVVAVQNERSQQKNRSVAWKILRAKLYDLEEKKRAA
CCCCCCCCCCCCCHHEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARDAAEALKKDIDFGSQIRSYVLQPYQMVKDLRTGVETGKVDDVLDGDLDPFIQPYLMGV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHH
RRTDRAVDE
HHHHHCCCC
>Mature Secondary Structure 
TQACAAVRRALDDAQALCELAEEARDEATRAEAAEAAEAVGRQLEGLELQKMLSGPHDA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCC
AGAIVEVKSGAGGVEAMDWAAMLYRMYVRYCERRGWEVEPADLVAGEEAGISSASFIVRG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCEEEEEC
DHPYGWLKAEKGVHRLVRISPFDQNARRQTSFAAVDVTPEIEDDIVIDIKEADVRIDTYR
CCCCCHHHHHHHHHHHEEECCCCCCHHHHCCEEEEECCCCCCCCEEEEEECCCEEEEECC
SSGAGGQKVNKTDSAVRMTHLPTGIVVAVQNERSQQKNRSVAWKILRAKLYDLEEKKRAA
CCCCCCCCCCCCCHHEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARDAAEALKKDIDFGSQIRSYVLQPYQMVKDLRTGVETGKVDDVLDGDLDPFIQPYLMGV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHH
RRTDRAVDE
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA