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The map label for this gene is folD [H]

Identifier: 220917811

GI number: 220917811

Start: 2991167

End: 2992015

Strand: Direct

Name: folD [H]

Synonym: A2cp1_2712

Alternate gene names: 220917811

Gene position: 2991167-2992015 (Clockwise)

Preceding gene: 220917806

Following gene: 220917812

Centisome position: 59.47

GC content: 72.67

Gene sequence:

>849_bases
ATGAACCTCATCGACGGCAAGCAGATCGCGGCGAAGGTGAAGGGCGAGGTGGCGACCGAGGTCCGCGCGCTGGCCGCCCG
CGGCGTGCAGACGGGCCTCACCGTGGTCCGGGTGGGCGACGACCCCGCCTCCGCGATCTACGTGCGCGGGAAGCGCAAGG
ACTGCGAGGAGGTTGGCATCACCTCGGTGGAGCACCACCTCCCGGCCACCACCACGCAGGCCGAGCTGCTCGCCCTCATC
GCGCGGCTGAACGCCGACCCGGCGGTGCACGGCATCCTGGTGCAGCTCCCGCTGCCGAAGCACGTGGACGAGCGCGCCGT
GCTCGACGCCATCTCGCCCGCCAAGGACGCGGACGGCTTCCATCCGTTCAACGTGGGCGCGCTCTCGATCGGCATCGCCG
GCGTGCCGCGCCCCTGCACGCCCGCCGGCGTGATGCGCATGCTCGACGAGGCGAAGGTCGATCCGAAGGGCAAGCGCGCG
CTGGTGGTCGGCCGCTCCAACATCGTCGGCAAGCCCATGGCGATGATGCTGCTCGAGCGGCACGCCACCGTGACCCTCGC
CCACTCCCGCACCGCGGATCTCGCCGGGGAGGTCGGCCGCGCCGACATCCTGGTGGCCGCCATCGGCAAGGCCGAGCTGG
TGAAGGGCGCCTGGGTCAAGGAGGGCGCGGTGGTGATCGACGTCGGCATGAACCGCCTCGCCGACGGGAAGCTGGTGGGC
GACGTGGAGTTCGCGGCGGCCGCCGCGCGCGCCTCGGCGATCACGCCGGTGCCGGGCGGCGTCGGCCCGATGACGCGCGC
CATGCTGCTCGTCAACACGGTGGAGCTGGCGAAGCGGACGGTGCGGTGA

Upstream 100 bases:

>100_bases
AGGGGTGGTCCGCCGAGCACGGTTCGCACGCGCCGGGCGCGCCACAAGCGTCCAGGATGGGGGACACGGGCGCGCGGCGT
GCTCTATACTCGCGCGCCTC

Downstream 100 bases:

>100_bases
GGCGCTGCGCCCGGGCGCTCGCGGCCATGGCCGTGGCGCTCGTGGGCGCCGCCGGCTGCTGCACCTGCGTCGACCTCGAC
TACACCTACCGGAGCGGCGC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MNLIDGKQIAAKVKGEVATEVRALAARGVQTGLTVVRVGDDPASAIYVRGKRKDCEEVGITSVEHHLPATTTQAELLALI
ARLNADPAVHGILVQLPLPKHVDERAVLDAISPAKDADGFHPFNVGALSIGIAGVPRPCTPAGVMRMLDEAKVDPKGKRA
LVVGRSNIVGKPMAMMLLERHATVTLAHSRTADLAGEVGRADILVAAIGKAELVKGAWVKEGAVVIDVGMNRLADGKLVG
DVEFAAAAARASAITPVPGGVGPMTRAMLLVNTVELAKRTVR

Sequences:

>Translated_282_residues
MNLIDGKQIAAKVKGEVATEVRALAARGVQTGLTVVRVGDDPASAIYVRGKRKDCEEVGITSVEHHLPATTTQAELLALI
ARLNADPAVHGILVQLPLPKHVDERAVLDAISPAKDADGFHPFNVGALSIGIAGVPRPCTPAGVMRMLDEAKVDPKGKRA
LVVGRSNIVGKPMAMMLLERHATVTLAHSRTADLAGEVGRADILVAAIGKAELVKGAWVKEGAVVIDVGMNRLADGKLVG
DVEFAAAAARASAITPVPGGVGPMTRAMLLVNTVELAKRTVR
>Mature_282_residues
MNLIDGKQIAAKVKGEVATEVRALAARGVQTGLTVVRVGDDPASAIYVRGKRKDCEEVGITSVEHHLPATTTQAELLALI
ARLNADPAVHGILVQLPLPKHVDERAVLDAISPAKDADGFHPFNVGALSIGIAGVPRPCTPAGVMRMLDEAKVDPKGKRA
LVVGRSNIVGKPMAMMLLERHATVTLAHSRTADLAGEVGRADILVAAIGKAELVKGAWVKEGAVVIDVGMNRLADGKLVG
DVEFAAAAARASAITPVPGGVGPMTRAMLLVNTVELAKRTVR

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=45.4861111111111, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=43.3898305084746, Blast_Score=201, Evalue=4e-52,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=44.2567567567568, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI36796743, Length=207, Percent_Identity=27.0531400966184, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786741, Length=274, Percent_Identity=55.1094890510949, Blast_Score=283, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17568735, Length=256, Percent_Identity=44.921875, Blast_Score=193, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=43.9446366782007, Blast_Score=243, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=44.4816053511706, Blast_Score=240, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=47.4576271186441, Blast_Score=234, Evalue=5e-62,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=47.4576271186441, Blast_Score=234, Evalue=6e-62,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=45.993031358885, Blast_Score=227, Evalue=7e-60,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=45.993031358885, Blast_Score=227, Evalue=7e-60,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=45.993031358885, Blast_Score=227, Evalue=8e-60,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=45.993031358885, Blast_Score=227, Evalue=8e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29367; Mature: 29367

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00766 THF_DHG_CYH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIDGKQIAAKVKGEVATEVRALAARGVQTGLTVVRVGDDPASAIYVRGKRKDCEEVGI
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCEEEEEECCCCCCEEEEECCCCCHHHHCC
TSVEHHLPATTTQAELLALIARLNADPAVHGILVQLPLPKHVDERAVLDAISPAKDADGF
CHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCCC
HPFNVGALSIGIAGVPRPCTPAGVMRMLDEAKVDPKGKRALVVGRSNIVGKPMAMMLLER
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHH
HATVTLAHSRTADLAGEVGRADILVAAIGKAELVKGAWVKEGAVVIDVGMNRLADGKLVG
CCEEEEECCCCHHHHCCCCCCEEEEEECCHHHHHCCCEEECCCEEEEECCCCCCCCCEEC
DVEFAAAAARASAITPVPGGVGPMTRAMLLVNTVELAKRTVR
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLIDGKQIAAKVKGEVATEVRALAARGVQTGLTVVRVGDDPASAIYVRGKRKDCEEVGI
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCEEEEEECCCCCCEEEEECCCCCHHHHCC
TSVEHHLPATTTQAELLALIARLNADPAVHGILVQLPLPKHVDERAVLDAISPAKDADGF
CHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCCC
HPFNVGALSIGIAGVPRPCTPAGVMRMLDEAKVDPKGKRALVVGRSNIVGKPMAMMLLER
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHH
HATVTLAHSRTADLAGEVGRADILVAAIGKAELVKGAWVKEGAVVIDVGMNRLADGKLVG
CCEEEEECCCCHHHHCCCCCCEEEEEECCHHHHHCCCEEECCCEEEEECCCCCCCCCEEC
DVEFAAAAARASAITPVPGGVGPMTRAMLLVNTVELAKRTVR
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA