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The map label for this gene is hmeA [H]

Identifier: 220917624

GI number: 220917624

Start: 2791094

End: 2792074

Strand: Reverse

Name: hmeA [H]

Synonym: A2cp1_2524

Alternate gene names: 220917624

Gene position: 2792074-2791094 (Counterclockwise)

Preceding gene: 220917625

Following gene: 220917623

Centisome position: 55.52

GC content: 69.32

Gene sequence:

>981_bases
ATGACCCCGAACAAGAGCCTGCCGGTCCTGGGCGGCGCGGACGCGCCGCCCCCGTCGCCGCGCGAAGGCATCGACCCCAT
CGGCGACGGCCCGCTCGACCCGGCGCGGCGCGCGTTCCTCCGCACCGCCGCCGCCGGCACCGCGGTCGCGGCGCTCGCGT
CGTGCAGCAGCGGCCTGGACATGGAGGACTTCCTCCGCAAGCACTTCAAGGAGCTCTCGCCGGACGAGCTGAAGAAGGTG
CTCGCCAAGGTGGAGAAGCGGAACCAGGAGCGCTTCGGGAAGAAGACCACCGTCTCCGCGAAGGGGCCGCAGCCCGGCGT
CGAGTACGGCTACGGCCTCGACATCTCCCGCTGCGTGGGCTGCCGCCGATGCGTCTACGCCTGCGTGAAGGAGAACAACC
AGTCCCGCACCGAGCCGCAGATCCACTGGATCCAGGTGCTGGAGATGGACAAGGAGCACGGCGTGGACCTGGCCCACGCC
GACCTCTACTACGAGGCCGACGCGGTGCCGCGCCCGGGCAAGTTCTACTTCCCGGTGCAGTGCCAGCAGTGCCGGAACCC
GCCCTGCGTGAAGGTCTGCCCGACGCAGGCCACGTGGAAGGAGCAGGACGGCATCGTGGTCGTGGACTACGACTGGTGCA
TCGGCTGCCGCTGCTGCATGTCGGCCTGCCCGTACGGCGCGCGCCACTTCAACTGGGCCACGCCGAACCTGCCGGCCGAG
GAGCTGAACCCGGACCAGCACTACCTCGGGAACCGCCCGCGCCCGAAGGGCGTGGTCGAGAAGTGCACCTTCTGCATCCA
GCGCGTGCGCGAGGGCCGCTACACGGCCTGCGTCGAGGTGTGCCCGGTGGGCGCCCGGAAGTTCGGGAACCTGCTCGATC
CCGAGAGCGAGATCCGCAAGGTGATGGAGACGAAGCGGGTCTTCGTCTTCAAGGAGGAGCTCGCCACCCACCCGCAGTTC
TTCTACTTCTACGCGACCTAG

Upstream 100 bases:

>100_bases
GCGTCCAGGAGATCGTCTCCGGCGGGAAGAAGACCGTGGCCCCGACCCTGACCTACCTCAAGCCCGAGCCGCGGCCGAAG
CGCCCGGAGGAGCAGCGCCG

Downstream 100 bases:

>100_bases
CGGAGAGGGCCCGACGATGTCCGGATTCCTCGAGTTCGTGAAGGGCTGCGTCCGCCAGGTGCTCTCGGGCGGCCGCGCCT
ACAAGGCCTGGATGGCGTTC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTPNKSLPVLGGADAPPPSPREGIDPIGDGPLDPARRAFLRTAAAGTAVAALASCSSGLDMEDFLRKHFKELSPDELKKV
LAKVEKRNQERFGKKTTVSAKGPQPGVEYGYGLDISRCVGCRRCVYACVKENNQSRTEPQIHWIQVLEMDKEHGVDLAHA
DLYYEADAVPRPGKFYFPVQCQQCRNPPCVKVCPTQATWKEQDGIVVVDYDWCIGCRCCMSACPYGARHFNWATPNLPAE
ELNPDQHYLGNRPRPKGVVEKCTFCIQRVREGRYTACVEVCPVGARKFGNLLDPESEIRKVMETKRVFVFKEELATHPQF
FYFYAT

Sequences:

>Translated_326_residues
MTPNKSLPVLGGADAPPPSPREGIDPIGDGPLDPARRAFLRTAAAGTAVAALASCSSGLDMEDFLRKHFKELSPDELKKV
LAKVEKRNQERFGKKTTVSAKGPQPGVEYGYGLDISRCVGCRRCVYACVKENNQSRTEPQIHWIQVLEMDKEHGVDLAHA
DLYYEADAVPRPGKFYFPVQCQQCRNPPCVKVCPTQATWKEQDGIVVVDYDWCIGCRCCMSACPYGARHFNWATPNLPAE
ELNPDQHYLGNRPRPKGVVEKCTFCIQRVREGRYTACVEVCPVGARKFGNLLDPESEIRKVMETKRVFVFKEELATHPQF
FYFYAT
>Mature_325_residues
TPNKSLPVLGGADAPPPSPREGIDPIGDGPLDPARRAFLRTAAAGTAVAALASCSSGLDMEDFLRKHFKELSPDELKKVL
AKVEKRNQERFGKKTTVSAKGPQPGVEYGYGLDISRCVGCRRCVYACVKENNQSRTEPQIHWIQVLEMDKEHGVDLAHAD
LYYEADAVPRPGKFYFPVQCQQCRNPPCVKVCPTQATWKEQDGIVVVDYDWCIGCRCCMSACPYGARHFNWATPNLPAEE
LNPDQHYLGNRPRPKGVVEKCTFCIQRVREGRYTACVEVCPVGARKFGNLLDPESEIRKVMETKRVFVFKEELATHPQFF
YFYAT

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787122, Length=197, Percent_Identity=34.010152284264, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787872, Length=197, Percent_Identity=34.5177664974619, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI2367345, Length=218, Percent_Identity=31.1926605504587, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787740, Length=133, Percent_Identity=36.8421052631579, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI226510944, Length=218, Percent_Identity=29.8165137614679, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787478, Length=132, Percent_Identity=35.6060606060606, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789370, Length=213, Percent_Identity=32.3943661971831, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787749, Length=182, Percent_Identity=31.8681318681319, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790326, Length=200, Percent_Identity=29, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787963, Length=179, Percent_Identity=29.608938547486, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082180, Length=175, Percent_Identity=28, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082179, Length=191, Percent_Identity=31.413612565445, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36553; Mature: 36422

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPNKSLPVLGGADAPPPSPREGIDPIGDGPLDPARRAFLRTAAAGTAVAALASCSSGLD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
MEDFLRKHFKELSPDELKKVLAKVEKRNQERFGKKTTVSAKGPQPGVEYGYGLDISRCVG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHH
CRRCVYACVKENNQSRTEPQIHWIQVLEMDKEHGVDLAHADLYYEADAVPRPGKFYFPVQ
HHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHHHCCCEEEHHEEEECCCCCCCCCEEECCC
CQQCRNPPCVKVCPTQATWKEQDGIVVVDYDWCIGCRCCMSACPYGARHFNWATPNLPAE
HHHCCCCCCEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
ELNPDQHYLGNRPRPKGVVEKCTFCIQRVREGRYTACVEVCPVGARKFGNLLDPESEIRK
HCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHHCCCCCCHHHHHH
VMETKRVFVFKEELATHPQFFYFYAT
HHHHHHHEEEHHHHCCCCEEEEEEEC
>Mature Secondary Structure 
TPNKSLPVLGGADAPPPSPREGIDPIGDGPLDPARRAFLRTAAAGTAVAALASCSSGLD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
MEDFLRKHFKELSPDELKKVLAKVEKRNQERFGKKTTVSAKGPQPGVEYGYGLDISRCVG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHH
CRRCVYACVKENNQSRTEPQIHWIQVLEMDKEHGVDLAHADLYYEADAVPRPGKFYFPVQ
HHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHHHCCCEEEHHEEEECCCCCCCCCEEECCC
CQQCRNPPCVKVCPTQATWKEQDGIVVVDYDWCIGCRCCMSACPYGARHFNWATPNLPAE
HHHCCCCCCEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
ELNPDQHYLGNRPRPKGVVEKCTFCIQRVREGRYTACVEVCPVGARKFGNLLDPESEIRK
HCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHHCCCCCCHHHHHH
VMETKRVFVFKEELATHPQFFYFYAT
HHHHHHHEEEHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]