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The map label for this gene is miaA

Identifier: 220917543

GI number: 220917543

Start: 2701395

End: 2702312

Strand: Reverse

Name: miaA

Synonym: A2cp1_2443

Alternate gene names: 220917543

Gene position: 2702312-2701395 (Counterclockwise)

Preceding gene: 220917544

Following gene: 220917542

Centisome position: 53.73

GC content: 78.65

Gene sequence:

>918_bases
ATGCGGCTGGTGGTGGTCGCCGGGCCGACCGCGTCCGGCAAGACCGCGCTCGCGATCGCGCTGGCGCGTCGGCTCGGCGG
CGAGATCGTCAACGCCGACTCGCAGCAGGTCTACCGCGGGCTCGACGTCGGGACCGCCAAGCCCACCGCCGAGGAGCGCG
GCGCCGCCCCGCACCACCTGCTCGACCTCGTCGAGCCGGGGGAGGGGATGGACGCGGCCCGCTTCGCGGCGCTCGCGGAT
GCCGCCATCGCCGGGATCGCCGCGCGCGGACGCGTGCCCATCGTGGCGGGAGGCACCGGGCTGTACGTCCGGGCCCTCCT
GCACGGCGTGGTGGAGGCGCCCGGGCGAGACCCGGCGCTCCGGGCGGCGCTGGAGGAGGAGGCGGCGCGACTGGGCCGGC
CGGCGGTCCACGCCCGGCTCGCCGCCGTGGACCCCGCCGCGGCGGAGCGCATCCGGCCCAACGACCTGGTGCGGGTGGTG
CGCGCGCTGGAGATCGCCGCCGGCGGCCGCACGCCCTCCGAGCTCTACCAGGCGCACGCGTTCCGCGAGGACCGCTACGA
CGCGGCGCTGCTCGCGCTCGACCCGCCGCGCGCCGAGCTGCACGCGCGCATCGACGCGCGCGTCCGCGCGATGTTCGCGG
GCGGCCTGCTCGACGAGGCGCGCGCGCTCGAGGCGCGCTTCGTCGGCGCGCTGCCGGCCCGGCTCCCCATCGGCTACGCG
GAGGCGGCGGCGCACCTGCGCGGCGAGCTCGATCTGGAGGAGGCCATCCGGCGCGTCCAGGTGGCGCACCGCCGCTACGC
GCGCCGCCAGGTGATCTGGCTCCGCAGGGAGCGCGGCGTCGCCTGGATCGCGCCGCCGCACGACGTCGAGGCGCTGTCGC
GCCGAGTCCTGGAGCCGCGACCTCCCGCGATCCGGTGA

Upstream 100 bases:

>100_bases
CGGCGGCGGCGCCGCTGCCGGAGGACGTCGAGCCGCTGCTCGCGCGCGCGGCGTCGCTCGTCCCCGGGCTGTTCGAGGCG
CGCGGCGCCGGGGCTCGCTG

Downstream 100 bases:

>100_bases
CCCGCGATCGCGGCACACGTCGCAGCCGATTTCGCCACACCCGCCTGATCTTCGTCACTCCGGCACACAGTCGACTACAG
TGCGGCCGCTGTCTCACGTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRLVVVAGPTASGKTALAIALARRLGGEIVNADSQQVYRGLDVGTAKPTAEERGAAPHHLLDLVEPGEGMDAARFAALAD
AAIAGIAARGRVPIVAGGTGLYVRALLHGVVEAPGRDPALRAALEEEAARLGRPAVHARLAAVDPAAAERIRPNDLVRVV
RALEIAAGGRTPSELYQAHAFREDRYDAALLALDPPRAELHARIDARVRAMFAGGLLDEARALEARFVGALPARLPIGYA
EAAAHLRGELDLEEAIRRVQVAHRRYARRQVIWLRRERGVAWIAPPHDVEALSRRVLEPRPPAIR

Sequences:

>Translated_305_residues
MRLVVVAGPTASGKTALAIALARRLGGEIVNADSQQVYRGLDVGTAKPTAEERGAAPHHLLDLVEPGEGMDAARFAALAD
AAIAGIAARGRVPIVAGGTGLYVRALLHGVVEAPGRDPALRAALEEEAARLGRPAVHARLAAVDPAAAERIRPNDLVRVV
RALEIAAGGRTPSELYQAHAFREDRYDAALLALDPPRAELHARIDARVRAMFAGGLLDEARALEARFVGALPARLPIGYA
EAAAHLRGELDLEEAIRRVQVAHRRYARRQVIWLRRERGVAWIAPPHDVEALSRRVLEPRPPAIR
>Mature_305_residues
MRLVVVAGPTASGKTALAIALARRLGGEIVNADSQQVYRGLDVGTAKPTAEERGAAPHHLLDLVEPGEGMDAARFAALAD
AAIAGIAARGRVPIVAGGTGLYVRALLHGVVEAPGRDPALRAALEEEAARLGRPAVHARLAAVDPAAAERIRPNDLVRVV
RALEIAAGGRTPSELYQAHAFREDRYDAALLALDPPRAELHARIDARVRAMFAGGLLDEARALEARFVGALPARLPIGYA
EAAAHLRGELDLEEAIRRVQVAHRRYARRQVIWLRRERGVAWIAPPHDVEALSRRVLEPRPPAIR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=346, Percent_Identity=32.9479768786127, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI1790613, Length=295, Percent_Identity=43.728813559322, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI25144712, Length=237, Percent_Identity=29.535864978903, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=31.8339100346021, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24649830, Length=271, Percent_Identity=35.0553505535055, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_ANAD2 (B8JBF4)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002492847.1
- ProteinModelPortal:   B8JBF4
- SMR:   B8JBF4
- GeneID:   7300103
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2443
- HOGENOM:   HBG559459
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 32708; Mature: 32708

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVVVAGPTASGKTALAIALARRLGGEIVNADSQQVYRGLDVGTAKPTAEERGAAPHHL
CEEEEEECCCCCCCHHHHHHHHHHHCCCEECCCHHHHHHCCCCCCCCCCHHHCCCCHHHH
LDLVEPGEGMDAARFAALADAAIAGIAARGRVPIVAGGTGLYVRALLHGVVEAPGRDPAL
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHH
RAALEEEAARLGRPAVHARLAAVDPAAAERIRPNDLVRVVRALEIAAGGRTPSELYQAHA
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FREDRYDAALLALDPPRAELHARIDARVRAMFAGGLLDEARALEARFVGALPARLPIGYA
HHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHH
EAAAHLRGELDLEEAIRRVQVAHRRYARRQVIWLRRERGVAWIAPPHDVEALSRRVLEPR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCHHHHHHHHCCCC
PPAIR
CCCCC
>Mature Secondary Structure
MRLVVVAGPTASGKTALAIALARRLGGEIVNADSQQVYRGLDVGTAKPTAEERGAAPHHL
CEEEEEECCCCCCCHHHHHHHHHHHCCCEECCCHHHHHHCCCCCCCCCCHHHCCCCHHHH
LDLVEPGEGMDAARFAALADAAIAGIAARGRVPIVAGGTGLYVRALLHGVVEAPGRDPAL
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHH
RAALEEEAARLGRPAVHARLAAVDPAAAERIRPNDLVRVVRALEIAAGGRTPSELYQAHA
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FREDRYDAALLALDPPRAELHARIDARVRAMFAGGLLDEARALEARFVGALPARLPIGYA
HHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHH
EAAAHLRGELDLEEAIRRVQVAHRRYARRQVIWLRRERGVAWIAPPHDVEALSRRVLEPR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCHHHHHHHHCCCC
PPAIR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA