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The map label for this gene is moeZ [H]

Identifier: 220917517

GI number: 220917517

Start: 2675328

End: 2676077

Strand: Reverse

Name: moeZ [H]

Synonym: A2cp1_2417

Alternate gene names: 220917517

Gene position: 2676077-2675328 (Counterclockwise)

Preceding gene: 220917518

Following gene: 220917516

Centisome position: 53.21

GC content: 77.6

Gene sequence:

>750_bases
ATGACCAGCCTCTCCGGCAAGCGCGTCCTCGTCATCGGCGCCGGCGGCCTCGGCGCCCCGGCCCTCCTCACGCTGGCGGC
GGCCGGGGTGGGGCAGCTCGTCCTGCTCGAGGACGACGCCGTCGAGACCTCGAACCTGAACCGGCAGCCGCTGTTCCAGG
AGGCCGACGTGGGCCGGCGCAAGGCGGCGGTCGCGGCCGCGCGGCTGCGGGCGATCTTCCCATCGGTCGCGGTGGACGCG
CGCGACGAGCGGTTCGACGCCGGCAACGCCGAGGCGCTGGCCGGCGGCGCCGACGTGATCGTGGACGGGTCGGACAACTT
CGAGACCAAGTTCCTCGCGAACGACGCCGCCACGCGCGTGAAGCGCCCGCTGGTGCACGCCGGCGTGCTCCGGACCACCG
TCCAGCTCCTCACCGTGGCCCCCTCCGGCCTGGGCGGCTGCCTGCGCTGCCTGTTCGAGGCGCCGCCGCCGCCGGGATCG
GTCGCGTCCTGCTCGCAGGCCGGGATCCTCGGCCCGGTGGCCGGGTTCGCCGGCGCGCTCCTTGCCGCCGAGGCGCTGCG
CCTGCTCGCCGGCGAGCGCGGGACGTACGAGGGGCGGCTGTTCACCTACGAGCTGCGCAGCGGGCGGTCGCGGCTCGTGC
TGGTCCGGAAGCGCCCCGGGTGCCTGGCCTGCGCCGGGCGCCAGGTGCTGGGGAGCAGCGCGCCCGCCGCCTGCGACGCA
CCGGCGGCGCCGGCGGGGGGCGCGGCGTGA

Upstream 100 bases:

>100_bases
CGCGGCGCGGGCGGGCAGGGCGCTCACCTTCTGACCGCCGCCGACGCGCCCCGGCGCTGTTGCCGGGGAGGCGGGGGCGG
GATATAGGCGAACGCGTCCC

Downstream 100 bases:

>100_bases
GCGGCCCCGAGCCCGCCCCGAGCCGGATCGACATCCGCGCCTACGCCTGCCCCATGACCTGGGTGAAGACGCGGATCGCG
CTGGAGCGGCTCGCGGAGGG

Product: UBA/THIF-type NAD/FAD binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTSLSGKRVLVIGAGGLGAPALLTLAAAGVGQLVLLEDDAVETSNLNRQPLFQEADVGRRKAAVAAARLRAIFPSVAVDA
RDERFDAGNAEALAGGADVIVDGSDNFETKFLANDAATRVKRPLVHAGVLRTTVQLLTVAPSGLGGCLRCLFEAPPPPGS
VASCSQAGILGPVAGFAGALLAAEALRLLAGERGTYEGRLFTYELRSGRSRLVLVRKRPGCLACAGRQVLGSSAPAACDA
PAAPAGGAA

Sequences:

>Translated_249_residues
MTSLSGKRVLVIGAGGLGAPALLTLAAAGVGQLVLLEDDAVETSNLNRQPLFQEADVGRRKAAVAAARLRAIFPSVAVDA
RDERFDAGNAEALAGGADVIVDGSDNFETKFLANDAATRVKRPLVHAGVLRTTVQLLTVAPSGLGGCLRCLFEAPPPPGS
VASCSQAGILGPVAGFAGALLAAEALRLLAGERGTYEGRLFTYELRSGRSRLVLVRKRPGCLACAGRQVLGSSAPAACDA
PAAPAGGAA
>Mature_248_residues
TSLSGKRVLVIGAGGLGAPALLTLAAAGVGQLVLLEDDAVETSNLNRQPLFQEADVGRRKAAVAAARLRAIFPSVAVDAR
DERFDAGNAEALAGGADVIVDGSDNFETKFLANDAATRVKRPLVHAGVLRTTVQLLTVAPSGLGGCLRCLFEAPPPPGSV
ASCSQAGILGPVAGFAGALLAAEALRLLAGERGTYEGRLFTYELRSGRSRLVLVRKRPGCLACAGRQVLGSSAPAACDAP
AAPAGGAA

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=220, Percent_Identity=41.8181818181818, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI38045942, Length=178, Percent_Identity=32.0224719101124, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI38045944, Length=178, Percent_Identity=32.0224719101124, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4885649, Length=148, Percent_Identity=30.4054054054054, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI87082356, Length=224, Percent_Identity=38.3928571428571, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787048, Length=222, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17540406, Length=223, Percent_Identity=35.4260089686099, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71984476, Length=112, Percent_Identity=36.6071428571429, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=189, Percent_Identity=39.1534391534392, Blast_Score=142, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6322639, Length=153, Percent_Identity=28.1045751633987, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582879, Length=229, Percent_Identity=37.9912663755458, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI20129973, Length=174, Percent_Identity=28.735632183908, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 25179; Mature: 25048

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLSGKRVLVIGAGGLGAPALLTLAAAGVGQLVLLEDDAVETSNLNRQPLFQEADVGRR
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCHH
KAAVAAARLRAIFPSVAVDARDERFDAGNAEALAGGADVIVDGSDNFETKFLANDAATRV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCEEEEECCCCCCEEEECCHHHHHH
KRPLVHAGVLRTTVQLLTVAPSGLGGCLRCLFEAPPPPGSVASCSQAGILGPVAGFAGAL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LAAEALRLLAGERGTYEGRLFTYELRSGRSRLVLVRKRPGCLACAGRQVLGSSAPAACDA
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEECCHHHHCCCCCCCCCC
PAAPAGGAA
CCCCCCCCC
>Mature Secondary Structure 
TSLSGKRVLVIGAGGLGAPALLTLAAAGVGQLVLLEDDAVETSNLNRQPLFQEADVGRR
CCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCHH
KAAVAAARLRAIFPSVAVDARDERFDAGNAEALAGGADVIVDGSDNFETKFLANDAATRV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCEEEEECCCCCCEEEECCHHHHHH
KRPLVHAGVLRTTVQLLTVAPSGLGGCLRCLFEAPPPPGSVASCSQAGILGPVAGFAGAL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LAAEALRLLAGERGTYEGRLFTYELRSGRSRLVLVRKRPGCLACAGRQVLGSSAPAACDA
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEECCHHHHCCCCCCCCCC
PAAPAGGAA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]