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The map label for this gene is rimK [H]

Identifier: 220917470

GI number: 220917470

Start: 2627247

End: 2628188

Strand: Reverse

Name: rimK [H]

Synonym: A2cp1_2370

Alternate gene names: 220917470

Gene position: 2628188-2627247 (Counterclockwise)

Preceding gene: 220917471

Following gene: 220917469

Centisome position: 52.26

GC content: 74.84

Gene sequence:

>942_bases
GTGCACCTCACGGTCCTGTCGCGCTCGAGCGAGATCTACACCACGCGCCGCCTGGTCGAGGCGGCGCGCGCCCGCGGGGT
CCGCTCGCGCGTGGTCGATCCGCTCGAGGTGGAGATGGGGCTGTTCGACCAGGCGCCGCAGGCCTACTGGCGCGGCAAGC
GCTTCCCCCGCACCGACGTGGTCGTGCCGCGGATCGGCCTGTCGATCCACCAGTACGGCCTCTCGGTGGTGAACCAGCTC
GAGCTGCTCGGCATCCCGGCGCTGAACGGCGCCTACGGCATCGCCGCCAGCCGCAACAAGATGCGCAGCCTCCAGATGCT
CTCCGCCGCCGGGGTGCCGGTGCCGCGCACCGTGATGGCGAGCGACCCCTCCGGCCTGAAGGACATGGCACGGCTGGTGG
GCGGCGTCCCGGTGCTGGTGAAGCTGCTCTCCACCAGCGAGAAGTCGGGCGTGATGATCTGCGAGACGCTCCAGTCGCTG
GAGGCGGCGCTCGAGGCCATCCTGGGGCTCGGCCAGAACATCGTGGTGCAGCAGTACCTGAAGGGCGCGAAGGGGCGGGA
CCTGCGCGCCCTGGTGGTGGGCGGCGAGGTCGTCGCGGCGATGCGCCGCCGGCCGCCGGTCGGCCGCTTCTCGCGCAACC
TGCGGCGCGGGGCGCAGTTCGAGCAGGTGAGCCTGCCGCCCGTCTACGCGCGCGCCGCCGCGCAGGCCGCCCGGGTGCTG
CAGCTCGAGGTCTGCGCGGTGGACATGCTCGACGTGAAGGGCACGCCGCGGGTCTTCGAGGTGAACTCCTCGCCGTCGAT
CCGCGAGGCGGAGAGCGCGTGCGGCGTGGACGCCGCGGGGCGCATCGTGGAGCGCGCCATGGCCCTGGTGCGCGGCGGGC
GCGCCCCGGCGGGCGCGGGGCGGCGGCGGCGCGACGGGGCGGCCGCCGCGGCGCGTCCCTGA

Upstream 100 bases:

>100_bases
CGGCCCGTCGTGCGTCCGGCGTCCGGCGTCCGGGCGGAGCGCCCGCGCCGAGCTCCAGCGCGCCTCGCTTTTCCGCCGCC
CCCGCCTTAATCTCGCGCCC

Downstream 100 bases:

>100_bases
ATGCGTGGCCGCCCCCGGCCGTCCCTGGTATAGAGCCCGACCATGCGCACCCTCGCACTCGCCCTCGCGCTCCTGCTCGC
CCCCGCGGCGTCGCGCGCCG

Product: RimK family alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MHLTVLSRSSEIYTTRRLVEAARARGVRSRVVDPLEVEMGLFDQAPQAYWRGKRFPRTDVVVPRIGLSIHQYGLSVVNQL
ELLGIPALNGAYGIAASRNKMRSLQMLSAAGVPVPRTVMASDPSGLKDMARLVGGVPVLVKLLSTSEKSGVMICETLQSL
EAALEAILGLGQNIVVQQYLKGAKGRDLRALVVGGEVVAAMRRRPPVGRFSRNLRRGAQFEQVSLPPVYARAAAQAARVL
QLEVCAVDMLDVKGTPRVFEVNSSPSIREAESACGVDAAGRIVERAMALVRGGRAPAGAGRRRRDGAAAAARP

Sequences:

>Translated_313_residues
MHLTVLSRSSEIYTTRRLVEAARARGVRSRVVDPLEVEMGLFDQAPQAYWRGKRFPRTDVVVPRIGLSIHQYGLSVVNQL
ELLGIPALNGAYGIAASRNKMRSLQMLSAAGVPVPRTVMASDPSGLKDMARLVGGVPVLVKLLSTSEKSGVMICETLQSL
EAALEAILGLGQNIVVQQYLKGAKGRDLRALVVGGEVVAAMRRRPPVGRFSRNLRRGAQFEQVSLPPVYARAAAQAARVL
QLEVCAVDMLDVKGTPRVFEVNSSPSIREAESACGVDAAGRIVERAMALVRGGRAPAGAGRRRRDGAAAAARP
>Mature_313_residues
MHLTVLSRSSEIYTTRRLVEAARARGVRSRVVDPLEVEMGLFDQAPQAYWRGKRFPRTDVVVPRIGLSIHQYGLSVVNQL
ELLGIPALNGAYGIAASRNKMRSLQMLSAAGVPVPRTVMASDPSGLKDMARLVGGVPVLVKLLSTSEKSGVMICETLQSL
EAALEAILGLGQNIVVQQYLKGAKGRDLRALVVGGEVVAAMRRRPPVGRFSRNLRRGAQFEQVSLPPVYARAAAQAARVL
QLEVCAVDMLDVKGTPRVFEVNSSPSIREAESACGVDAAGRIVERAMALVRGGRAPAGAGRRRRDGAAAAARP

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=223, Percent_Identity=26.9058295964126, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI134254462, Length=227, Percent_Identity=29.0748898678414, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787076, Length=283, Percent_Identity=39.9293286219081, Blast_Score=217, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33536; Mature: 33536

Theoretical pI: Translated: 11.42; Mature: 11.42

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLTVLSRSSEIYTTRRLVEAARARGVRSRVVDPLEVEMGLFDQAPQAYWRGKRFPRTDV
CEEEEECCCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHCHHCCCCHHHHCCCCCCCCCE
VVPRIGLSIHQYGLSVVNQLELLGIPALNGAYGIAASRNKMRSLQMLSAAGVPVPRTVMA
EECHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEC
SDPSGLKDMARLVGGVPVLVKLLSTSEKSGVMICETLQSLEAALEAILGLGQNIVVQQYL
CCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KGAKGRDLRALVVGGEVVAAMRRRPPVGRFSRNLRRGAQFEQVSLPPVYARAAAQAARVL
CCCCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
QLEVCAVDMLDVKGTPRVFEVNSSPSIREAESACGVDAAGRIVERAMALVRGGRAPAGAG
HHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
RRRRDGAAAAARP
CCCCCCCCCCCCC
>Mature Secondary Structure
MHLTVLSRSSEIYTTRRLVEAARARGVRSRVVDPLEVEMGLFDQAPQAYWRGKRFPRTDV
CEEEEECCCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHCHHCCCCHHHHCCCCCCCCCE
VVPRIGLSIHQYGLSVVNQLELLGIPALNGAYGIAASRNKMRSLQMLSAAGVPVPRTVMA
EECHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEC
SDPSGLKDMARLVGGVPVLVKLLSTSEKSGVMICETLQSLEAALEAILGLGQNIVVQQYL
CCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KGAKGRDLRALVVGGEVVAAMRRRPPVGRFSRNLRRGAQFEQVSLPPVYARAAAQAARVL
CCCCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
QLEVCAVDMLDVKGTPRVFEVNSSPSIREAESACGVDAAGRIVERAMALVRGGRAPAGAG
HHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
RRRRDGAAAAARP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA