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The map label for this gene is tuf1 [H]

Identifier: 220917467

GI number: 220917467

Start: 2624207

End: 2625397

Strand: Reverse

Name: tuf1 [H]

Synonym: A2cp1_2367

Alternate gene names: 220917467

Gene position: 2625397-2624207 (Counterclockwise)

Preceding gene: 220917468

Following gene: 220917466

Centisome position: 52.2

GC content: 67.76

Gene sequence:

>1191_bases
ATGGCCAAGGAGAAGTTCGAGCGCAGCAAGCCGCACGTGAACGTCGGGACGATCGGGCACGTGGACCACGGCAAGACGAC
GCTGACGGCGGCGATCACGAAGGTGCTGGCGCAGAAGGGCGGGGCGCAGTTCCTGGCGTACGACCAGATCGACAAGGCGC
CGGAGGAGCGGGAGCGCGGCATCACGATCGCGACGGCGCACGTGGAGTACCAGACGGAGAAGCGGCACTACGCGCACGTC
GACTGCCCGGGGCACGCGGACTACGTGAAGAACATGATCACCGGGGCGGCGCAGATGGACGGCGCGATCCTGGTGGTGTC
GGCGGCGGACGGTCCGATGCCGCAGACGCGGGAGCACATCCTGCTGGCCCGTCAGGTGGGCGTGCCGTACATCGTGGTCT
TCCTGAACAAGGTGGACATGGTGGACGACAAGGAGCTCCTGGACCTGGTGGAGCTGGAGGTCCGGGAGCTGCTGAGCGAG
TACGACTTCCCGGGCAACGAGATCCCGATCGTGAAGGGGTCGGCGCTGAAGGCGCTCGAGGGCGACAAGGGCGAGCTCGG
GGAGCAGGCGATCTTCAAGCTGATGGACGCGGTGGACGCGTACATTCCGACGCCGCAGCGCGCGACGGACAAGCCGTTCC
TGATGCCGGTCGAGGACGTGTTCTCGATCTCGGGCCGCGGGACGGTGGCGACGGGCCGCGTGGAGCGCGGGATCGTGAAG
GTGGGCGAGGAGATCGAGGTCGTCGGGCTGAAGGCGACGGCGAAGACGGTGGTGACCGGCGTCGAGATGTTCCGCAAGCT
GCTGGACGAGGGGCGCGCGGGCGACAACATCGGGGCGCTGCTGCGCGGCCTGAAGCGCGAGGAGGTGGAGCGCGGTCAGG
TGCTGGCGAAGCCGGGGTCGATCACGCCGCACACGAAGTTCAAGGCCGAGGTGTACGTGCTGACGAAGGAGGAGGGTGGT
CGTCACACCCCGTTCTTCAACGGCTACCGGCCGCAGTTCTACTTCCGGACGACGGACGTGACGGGGTCGGTGCAGCTGCC
GCAGGGAGTCGAGATGGTGATGCCGGGCGACAACATCGGGATGGAGGTGGAGCTGATCACCCCCATCGCGATGGAGAAGG
AGCTCCGCTTCGCCATCCGCGAGGGCGGCCGCACGGTCGGCGCGGGCGTGGTCGCCGAGGTCATCCAGTAA

Upstream 100 bases:

>100_bases
GGACGAGGTCTCCAGTTCAATCCTGGACGAGGGCTCTCAGTCACTCCGTCGCGGTAGCCGAGTCATCCGGCGCACGCGCC
AACAGCAGGGAGCGTACCCA

Downstream 100 bases:

>100_bases
GGAAAGAAGGACGTCATGGCCGGCAATCGCAGCATCATCACGCTCGAGTGCAAGACCTGCAAAGAGCGGAACTACACGAC
CACCAAGAACAAGAAGAAGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERSKPHVNVGTIGHVDHGKTTLTAAITKVLAQKGGAQFLAYDQIDKAPEERERGITIATAHVEYQTEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDMVDDKELLDLVELEVRELLSE
YDFPGNEIPIVKGSALKALEGDKGELGEQAIFKLMDAVDAYIPTPQRATDKPFLMPVEDVFSISGRGTVATGRVERGIVK
VGEEIEVVGLKATAKTVVTGVEMFRKLLDEGRAGDNIGALLRGLKREEVERGQVLAKPGSITPHTKFKAEVYVLTKEEGG
RHTPFFNGYRPQFYFRTTDVTGSVQLPQGVEMVMPGDNIGMEVELITPIAMEKELRFAIREGGRTVGAGVVAEVIQ

Sequences:

>Translated_396_residues
MAKEKFERSKPHVNVGTIGHVDHGKTTLTAAITKVLAQKGGAQFLAYDQIDKAPEERERGITIATAHVEYQTEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDMVDDKELLDLVELEVRELLSE
YDFPGNEIPIVKGSALKALEGDKGELGEQAIFKLMDAVDAYIPTPQRATDKPFLMPVEDVFSISGRGTVATGRVERGIVK
VGEEIEVVGLKATAKTVVTGVEMFRKLLDEGRAGDNIGALLRGLKREEVERGQVLAKPGSITPHTKFKAEVYVLTKEEGG
RHTPFFNGYRPQFYFRTTDVTGSVQLPQGVEMVMPGDNIGMEVELITPIAMEKELRFAIREGGRTVGAGVVAEVIQ
>Mature_395_residues
AKEKFERSKPHVNVGTIGHVDHGKTTLTAAITKVLAQKGGAQFLAYDQIDKAPEERERGITIATAHVEYQTEKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDMVDDKELLDLVELEVRELLSEY
DFPGNEIPIVKGSALKALEGDKGELGEQAIFKLMDAVDAYIPTPQRATDKPFLMPVEDVFSISGRGTVATGRVERGIVKV
GEEIEVVGLKATAKTVVTGVEMFRKLLDEGRAGDNIGALLRGLKREEVERGQVLAKPGSITPHTKFKAEVYVLTKEEGGR
HTPFFNGYRPQFYFRTTDVTGSVQLPQGVEMVMPGDNIGMEVELITPIAMEKELRFAIREGGRTVGAGVVAEVIQ

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=59.6446700507614, Blast_Score=498, Evalue=1e-141,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=30, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=30, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=29.0178571428571, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=29.0178571428571, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=28.695652173913, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI194018520, Length=341, Percent_Identity=29.3255131964809, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI194097354, Length=341, Percent_Identity=29.3255131964809, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI194018522, Length=340, Percent_Identity=29.1176470588235, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI46094014, Length=354, Percent_Identity=28.8135593220339, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI4503507, Length=273, Percent_Identity=28.9377289377289, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI157426893, Length=256, Percent_Identity=30.078125, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=77.9746835443038, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=77.7777777777778, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI2367247, Length=378, Percent_Identity=29.1005291005291, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789108, Length=323, Percent_Identity=30.6501547987616, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788922, Length=132, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.1328320802005, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.9276485788114, Blast_Score=344, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=30.1339285714286, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=30.1339285714286, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=419, Percent_Identity=30.0715990453461, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=28.7037037037037, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=431, Percent_Identity=28.538283062645, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=453, Percent_Identity=27.8145695364238, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=29.7658862876254, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=173, Percent_Identity=36.4161849710983, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=29.7872340425532, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=68.1818181818182, Blast_Score=559, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=31.3063063063063, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=31.3063063063063, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=459, Percent_Identity=24.6187363834423, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=341, Percent_Identity=30.2052785923754, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=286, Percent_Identity=26.9230769230769, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=30.8641975308642, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=199, Percent_Identity=29.1457286432161, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.5419847328244, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.5419847328244, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=50, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=30.4824561403509, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=30.4824561403509, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=30.4824561403509, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=30.4824561403509, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=30.989010989011, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=30.989010989011, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=33.0065359477124, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=27.7404921700224, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17137380, Length=349, Percent_Identity=29.7994269340974, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24647046, Length=281, Percent_Identity=27.7580071174377, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24647048, Length=281, Percent_Identity=27.7580071174377, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI78706572, Length=134, Percent_Identity=35.0746268656716, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43331; Mature: 43200

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERSKPHVNVGTIGHVDHGKTTLTAAITKVLAQKGGAQFLAYDQIDKAPEERERG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITIATAHVEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKVDMVDDKELLDLVELEVRELLSEYDFPGNEIPIVKGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHCCC
GDKGELGEQAIFKLMDAVDAYIPTPQRATDKPFLMPVEDVFSISGRGTVATGRVERGIVK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCCCCCEEEECHHHHHHHH
VGEEIEVVGLKATAKTVVTGVEMFRKLLDEGRAGDNIGALLRGLKREEVERGQVLAKPGS
CCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
ITPHTKFKAEVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGSVQLPQGVEMVMPGDNIG
CCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCCC
MEVELITPIAMEKELRFAIREGGRTVGAGVVAEVIQ
EEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHC
>Mature Secondary Structure 
AKEKFERSKPHVNVGTIGHVDHGKTTLTAAITKVLAQKGGAQFLAYDQIDKAPEERERG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITIATAHVEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKVDMVDDKELLDLVELEVRELLSEYDFPGNEIPIVKGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHCCC
GDKGELGEQAIFKLMDAVDAYIPTPQRATDKPFLMPVEDVFSISGRGTVATGRVERGIVK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCCCCCEEEECHHHHHHHH
VGEEIEVVGLKATAKTVVTGVEMFRKLLDEGRAGDNIGALLRGLKREEVERGQVLAKPGS
CCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
ITPHTKFKAEVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGSVQLPQGVEMVMPGDNIG
CCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCCC
MEVELITPIAMEKELRFAIREGGRTVGAGVVAEVIQ
EEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA