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The map label for this gene is gcvH [H]

Identifier: 220917062

GI number: 220917062

Start: 2212639

End: 2213361

Strand: Direct

Name: gcvH [H]

Synonym: A2cp1_1958

Alternate gene names: 220917062

Gene position: 2212639-2213361 (Clockwise)

Preceding gene: 220917061

Following gene: 220917063

Centisome position: 43.99

GC content: 74.69

Gene sequence:

>723_bases
ATGGCCTACGACCTGCTCTCCGTGTATCCGGCCAAGCTCCTCGAGTACGGACTCGGGATCGCCTACCTGCTGATGTTCAT
CCCGTTCTGGCGCTACGTCCAGGGCGGCAAGCCGGCGGCGGCCGAGGTCCGCGCCCCCGCCCGCGTCCCCGCGGTGGCGC
CCGCGGCCCACCCGATCGCGGCCCGGCCCGCGGTGGCTGGCTGGTTCCAGGTGCCCGCCGGCGTGCACCTGCACCCCGGC
CACACCTGGGCGCGCCTGGAGTCCGACGGCCTGGTGGCGGTCGGCGTGGACGACTTCGCCCACAAGCTGGTGGGGCCGGC
CCGGGTCGAGCTGCCCAGCGTCGGCGCCAAGGTCGCGCAGGGCGAGCCGGCGCTCGAGCTCGGCGACGGCGAGCGCAGCG
TCCCGATGCTCTCGCCCATCGACGGGACCGTGGTGGCGGTGAACGCGGCGGCGCGCGAGCGGACGGACGGCGTCGAGGAT
CCGTACGGCGCCGGCTGGCTGTTCAAGGTGAAGGCGCCCCGCCTCGCCGCGAACCTCCGCCAGCTCTTCACCGACAGCGC
CGCCAACCGGTTCCTGGAGGACGCGTCCGAGCGCCTCGCCATGCGGATGAGCCCCGAGCTCGGCCGCGTGCTGCAGGACG
GCGGCGTGCCCATCCACGGCATCGGCCGCGCGCTGGCCGGCGACGACTGGGACGAGATGGCGCGCACCTTCTTCCTGACC
TGA

Upstream 100 bases:

>100_bases
CGTCACCATCGGCGTGCTGGCGTACCGCTGGATCGTCAACCGCATGCCCATCCTGCGTGGGGACCCGGCGTTCCCCGCTG
AGCACTGAAAGGAGGAAGCC

Downstream 100 bases:

>100_bases
GAACGGAGATCACAGCCATGACGCTCAAGCTCTCGTGGTGCGGTGCGCTGGCCGGGCTGCTGCTGGGCGGCAGCGCGCTC
GCGGGCGACCTCCCGAACCT

Product: glycine cleavage H-protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MAYDLLSVYPAKLLEYGLGIAYLLMFIPFWRYVQGGKPAAAEVRAPARVPAVAPAAHPIAARPAVAGWFQVPAGVHLHPG
HTWARLESDGLVAVGVDDFAHKLVGPARVELPSVGAKVAQGEPALELGDGERSVPMLSPIDGTVVAVNAAARERTDGVED
PYGAGWLFKVKAPRLAANLRQLFTDSAANRFLEDASERLAMRMSPELGRVLQDGGVPIHGIGRALAGDDWDEMARTFFLT

Sequences:

>Translated_240_residues
MAYDLLSVYPAKLLEYGLGIAYLLMFIPFWRYVQGGKPAAAEVRAPARVPAVAPAAHPIAARPAVAGWFQVPAGVHLHPG
HTWARLESDGLVAVGVDDFAHKLVGPARVELPSVGAKVAQGEPALELGDGERSVPMLSPIDGTVVAVNAAARERTDGVED
PYGAGWLFKVKAPRLAANLRQLFTDSAANRFLEDASERLAMRMSPELGRVLQDGGVPIHGIGRALAGDDWDEMARTFFLT
>Mature_239_residues
AYDLLSVYPAKLLEYGLGIAYLLMFIPFWRYVQGGKPAAAEVRAPARVPAVAPAAHPIAARPAVAGWFQVPAGVHLHPGH
TWARLESDGLVAVGVDDFAHKLVGPARVELPSVGAKVAQGEPALELGDGERSVPMLSPIDGTVVAVNAAARERTDGVEDP
YGAGWLFKVKAPRLAANLRQLFTDSAANRFLEDASERLAMRMSPELGRVLQDGGVPIHGIGRALAGDDWDEMARTFFLT

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=34.6456692913386, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 25638; Mature: 25506

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDLLSVYPAKLLEYGLGIAYLLMFIPFWRYVQGGKPAAAEVRAPARVPAVAPAAHPIA
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCC
ARPAVAGWFQVPAGVHLHPGHTWARLESDGLVAVGVDDFAHKLVGPARVELPSVGAKVAQ
CCCCHHHHEECCCCCEECCCCCEEEECCCCEEEECHHHHHHHHCCCCCEECCCCCCHHCC
GEPALELGDGERSVPMLSPIDGTVVAVNAAARERTDGVEDPYGAGWLFKVKAPRLAANLR
CCCCEEECCCCCCCCEECCCCCEEEEEECHHHHHCCCCCCCCCCCEEEEECCCHHHHHHH
QLFTDSAANRFLEDASERLAMRMSPELGRVLQDGGVPIHGIGRALAGDDWDEMARTFFLT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
AYDLLSVYPAKLLEYGLGIAYLLMFIPFWRYVQGGKPAAAEVRAPARVPAVAPAAHPIA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCC
ARPAVAGWFQVPAGVHLHPGHTWARLESDGLVAVGVDDFAHKLVGPARVELPSVGAKVAQ
CCCCHHHHEECCCCCEECCCCCEEEECCCCEEEECHHHHHHHHCCCCCEECCCCCCHHCC
GEPALELGDGERSVPMLSPIDGTVVAVNAAARERTDGVEDPYGAGWLFKVKAPRLAANLR
CCCCEEECCCCCCCCEECCCCCEEEEEECHHHHHCCCCCCCCCCCEEEEECCCHHHHHHH
QLFTDSAANRFLEDASERLAMRMSPELGRVLQDGGVPIHGIGRALAGDDWDEMARTFFLT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]