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The map label for this gene is fumB [H]

Identifier: 220916992

GI number: 220916992

Start: 2118906

End: 2120528

Strand: Direct

Name: fumB [H]

Synonym: A2cp1_1888

Alternate gene names: 220916992

Gene position: 2118906-2120528 (Clockwise)

Preceding gene: 220916991

Following gene: 220916999

Centisome position: 42.13

GC content: 67.1

Gene sequence:

>1623_bases
ATGGCGACTCCCGCGTTCCACTACCAGGACCCGTTCCCCGTCTCGAAGGACGCGACCAAGTACCGGCTCCTCACCAGCGA
GGGCGTGTCGGTCGGGACGTTCGAGGGCAAGGAGATCCTGAAGGTCGAGCCGGCGGCGCTCACCCGCCTCGCGCGCGAGG
CGATGCGCGAGGTGTCGTTCTTCCTCCGCCCCGAGCACAACGAGCAGGTCGCGAAGATCCTGGCAGACCCCGAGGCGTCG
CAGAACGACAAGGGCGTCGCCATCGCGTTCCTCAAGAACGCCGAGATCGCGGCCCGCGGCGAGCTGCCCATCTGCCAGGA
CACCGGCACCGCCACCATCGTGGCGAAGAAGGGCCAGCAGGTCTGGACCGGCGTGAAGGACGAGCAGTACCTGTCGGAGG
GCGTGTACCTCACCTACACCGGCGAGAACCTCCGCTACTCCCAGACCTCCGCGCTCGACATGTACCGCGAGGTGAACACC
GGCACGAACCTGCCGGCGCAGATCGACGTCCTCGCGACCGAGGGCGCCGAGTACAAGTTCCTGTTCGTGGCGAAGGGCGG
CGGGTCGGCCAACAAGACCATGCTGTTCCAGGAGACCAAGGCGCTCCTCACGCCGGAGAAGCTCGAGAAGTTCCTGGTCG
ACAAGATGAAGTACCTCGGCACCGCCGCCTGCCCGCCGTACCACATCGCGGTGGTGGTGGGCGGCACGAGCGCCGAGACC
TGCCTGAAGACGGTGAAGCTCGCCTCGACGAAGTACTACGACGGCCTCCCCACCGAGGGGAACGAGCACGGCCAGGCGTT
CCGCGACGTCGCGCTGGAGCAGCGGCTGCTCGAGGCGGCCAACAAGCTCGGCATCGGCGCGCAGTTCGGCGGCAAGTACT
TCGCGCACGACATCCGCGTGGTGCGGCTGCCGCGCCACGGCGCCTCCTGCCCGGTCGGCATGGCGGTGTCCTGCTCGGCC
GACCGCAACGTGAAGGCGAAGATCAACAGGGAAGGGCTCTGGATCGAGGAGCTCGACCACGCGCCGGGCCGCCTCATCCC
GCTCGAGCTGAAGAAGGGCAAGCACGAGCACGGCGTGAAGGTGGACCTCAACAAGCCCATGAAGGAGATCCTGGCCGAGC
TGACGAAGTACCCGGTCTCGACCCCGCTGCTCCTCACCGGCACGCTGGTGGTGGCCCGCGACATCGCGCACGCGAAGTTC
AAGGAGCTGATCGACCAGGGCAAGGGCGTGCCCGAGTACCTGAAGCACCACCCGGTGTACTACGCCGGCCCGGCCAAGAC
GCCGAAGGGCAAGCCGTCCGGCTCGTTCGGCCCCACCACCGCCGGCCGCATGGACTCGTACGTGGACCTGCTGCAGTCGC
ACGGCGCGTCGATGGTGATGATCGCGAAGGGCAACCGCAGCCAGCAGGTGACCGACGCGTGCAAGAAGCACGGCGGCTTC
TACCTGGGCTCGATCGGCGGCCCGGCCGCGGTGCTGGCCGAGGAGAACATCAAGAAGGTGGAGTGCATCGACTTCGCCGA
GCTCGGCATGGAAGCCGTCTGGAAGATCGAGGTCGAGAACTTCCCCGCGTTCATCCTGGTGGACGACAAGGGCAACGACT
TCTTCAAGAAGCTGGGGATGTAG

Upstream 100 bases:

>100_bases
CGGCCGCCGTCCTGTGCTTTCCTGCGGGCCCCGGGCACCGAGAGCGCACCGGCCCGCGTTTCGACTTCCCACCCCGTTCG
CCCGCGAAGGAGAGGCACAC

Downstream 100 bases:

>100_bases
CCGGCCCCGCCTCGATCACCCGCACCCCCCGGCCGGCAGGCGCGCCCGCGAAGGCCGCCTGCCGGCCGTCCTGTTCCGGG
GCCGCCGCGGCGGCCGGGGG

Product: hydro-lyase, Fe-S type, tartrate/fumarate subfamily subunit beta

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MATPAFHYQDPFPVSKDATKYRLLTSEGVSVGTFEGKEILKVEPAALTRLAREAMREVSFFLRPEHNEQVAKILADPEAS
QNDKGVAIAFLKNAEIAARGELPICQDTGTATIVAKKGQQVWTGVKDEQYLSEGVYLTYTGENLRYSQTSALDMYREVNT
GTNLPAQIDVLATEGAEYKFLFVAKGGGSANKTMLFQETKALLTPEKLEKFLVDKMKYLGTAACPPYHIAVVVGGTSAET
CLKTVKLASTKYYDGLPTEGNEHGQAFRDVALEQRLLEAANKLGIGAQFGGKYFAHDIRVVRLPRHGASCPVGMAVSCSA
DRNVKAKINREGLWIEELDHAPGRLIPLELKKGKHEHGVKVDLNKPMKEILAELTKYPVSTPLLLTGTLVVARDIAHAKF
KELIDQGKGVPEYLKHHPVYYAGPAKTPKGKPSGSFGPTTAGRMDSYVDLLQSHGASMVMIAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAEENIKKVECIDFAELGMEAVWKIEVENFPAFILVDDKGNDFFKKLGM

Sequences:

>Translated_540_residues
MATPAFHYQDPFPVSKDATKYRLLTSEGVSVGTFEGKEILKVEPAALTRLAREAMREVSFFLRPEHNEQVAKILADPEAS
QNDKGVAIAFLKNAEIAARGELPICQDTGTATIVAKKGQQVWTGVKDEQYLSEGVYLTYTGENLRYSQTSALDMYREVNT
GTNLPAQIDVLATEGAEYKFLFVAKGGGSANKTMLFQETKALLTPEKLEKFLVDKMKYLGTAACPPYHIAVVVGGTSAET
CLKTVKLASTKYYDGLPTEGNEHGQAFRDVALEQRLLEAANKLGIGAQFGGKYFAHDIRVVRLPRHGASCPVGMAVSCSA
DRNVKAKINREGLWIEELDHAPGRLIPLELKKGKHEHGVKVDLNKPMKEILAELTKYPVSTPLLLTGTLVVARDIAHAKF
KELIDQGKGVPEYLKHHPVYYAGPAKTPKGKPSGSFGPTTAGRMDSYVDLLQSHGASMVMIAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAEENIKKVECIDFAELGMEAVWKIEVENFPAFILVDDKGNDFFKKLGM
>Mature_539_residues
ATPAFHYQDPFPVSKDATKYRLLTSEGVSVGTFEGKEILKVEPAALTRLAREAMREVSFFLRPEHNEQVAKILADPEASQ
NDKGVAIAFLKNAEIAARGELPICQDTGTATIVAKKGQQVWTGVKDEQYLSEGVYLTYTGENLRYSQTSALDMYREVNTG
TNLPAQIDVLATEGAEYKFLFVAKGGGSANKTMLFQETKALLTPEKLEKFLVDKMKYLGTAACPPYHIAVVVGGTSAETC
LKTVKLASTKYYDGLPTEGNEHGQAFRDVALEQRLLEAANKLGIGAQFGGKYFAHDIRVVRLPRHGASCPVGMAVSCSAD
RNVKAKINREGLWIEELDHAPGRLIPLELKKGKHEHGVKVDLNKPMKEILAELTKYPVSTPLLLTGTLVVARDIAHAKFK
ELIDQGKGVPEYLKHHPVYYAGPAKTPKGKPSGSFGPTTAGRMDSYVDLLQSHGASMVMIAKGNRSQQVTDACKKHGGFY
LGSIGGPAAVLAEENIKKVECIDFAELGMEAVWKIEVENFPAFILVDDKGNDFFKKLGM

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=538, Percent_Identity=74.5353159851301, Blast_Score=845, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=540, Percent_Identity=72.4074074074074, Blast_Score=831, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=180, Percent_Identity=30.5555555555556, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 58939; Mature: 58808

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPAFHYQDPFPVSKDATKYRLLTSEGVSVGTFEGKEILKVEPAALTRLAREAMREVSF
CCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHH
FLRPEHNEQVAKILADPEASQNDKGVAIAFLKNAEIAARGELPICQDTGTATIVAKKGQQ
HCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEECCCCCEECCCCCEEEEEECCCH
VWTGVKDEQYLSEGVYLTYTGENLRYSQTSALDMYREVNTGTNLPAQIDVLATEGAEYKF
HHCCCCCHHHHHCCEEEEECCCCCEECCHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEE
LFVAKGGGSANKTMLFQETKALLTPEKLEKFLVDKMKYLGTAACPPYHIAVVVGGTSAET
EEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHH
CLKTVKLASTKYYDGLPTEGNEHGQAFRDVALEQRLLEAANKLGIGAQFGGKYFAHDIRV
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECEEE
VRLPRHGASCPVGMAVSCSADRNVKAKINREGLWIEELDHAPGRLIPLELKKGKHEHGVK
EEECCCCCCCCCCEEEECCCCCCCEEEECCCCCCHHHHCCCCCCEEEEEECCCCCCCCEE
VDLNKPMKEILAELTKYPVSTPLLLTGTLVVARDIAHAKFKELIDQGKGVPEYLKHHPVY
EECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEE
YAGPAKTPKGKPSGSFGPTTAGRMDSYVDLLQSHGASMVMIAKGNRSQQVTDACKKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAEENIKKVECIDFAELGMEAVWKIEVENFPAFILVDDKGNDFFKKLGM
EEECCCCCEEEEECCCCCEEEECCHHHCCCEEEEEEEECCCCEEEEEECCCCHHHHHHCC
>Mature Secondary Structure 
ATPAFHYQDPFPVSKDATKYRLLTSEGVSVGTFEGKEILKVEPAALTRLAREAMREVSF
CCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHH
FLRPEHNEQVAKILADPEASQNDKGVAIAFLKNAEIAARGELPICQDTGTATIVAKKGQQ
HCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEECCCCCEECCCCCEEEEEECCCH
VWTGVKDEQYLSEGVYLTYTGENLRYSQTSALDMYREVNTGTNLPAQIDVLATEGAEYKF
HHCCCCCHHHHHCCEEEEECCCCCEECCHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEE
LFVAKGGGSANKTMLFQETKALLTPEKLEKFLVDKMKYLGTAACPPYHIAVVVGGTSAET
EEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHH
CLKTVKLASTKYYDGLPTEGNEHGQAFRDVALEQRLLEAANKLGIGAQFGGKYFAHDIRV
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECEEE
VRLPRHGASCPVGMAVSCSADRNVKAKINREGLWIEELDHAPGRLIPLELKKGKHEHGVK
EEECCCCCCCCCCEEEECCCCCCCEEEECCCCCCHHHHCCCCCCEEEEEECCCCCCCCEE
VDLNKPMKEILAELTKYPVSTPLLLTGTLVVARDIAHAKFKELIDQGKGVPEYLKHHPVY
EECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEE
YAGPAKTPKGKPSGSFGPTTAGRMDSYVDLLQSHGASMVMIAKGNRSQQVTDACKKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAEENIKKVECIDFAELGMEAVWKIEVENFPAFILVDDKGNDFFKKLGM
EEECCCCCEEEEECCCCCEEEECCHHHCCCEEEEEEEECCCCEEEEEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]