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The map label for this gene is leuB [H]

Identifier: 220916990

GI number: 220916990

Start: 2116502

End: 2117563

Strand: Direct

Name: leuB [H]

Synonym: A2cp1_1886

Alternate gene names: 220916990

Gene position: 2116502-2117563 (Clockwise)

Preceding gene: 220916989

Following gene: 220916991

Centisome position: 42.08

GC content: 73.07

Gene sequence:

>1062_bases
GTGAACGCGAAGATCGTGGTGCTGCCGGGCGACGGGATCGGGCCGGAGGTGACGGCCGAGGCGGTGCGGGTGCTGGAGGC
GGTGGCGCGCCGGGGAGGGCACACGCTCTCGTTCACCGAGCGGCTGATGGGCGGGTGCTCCATCGACGCGCACGGGACCG
CGCTCACGCCCGAGGTGCTCGCCGACTGCCAGGCGGCCGACGCGGTGCTGCTCGGCGCCGTGGGCGGGCCGAAGTGGGAC
GACCCGCGCGCCAGGGTGCGCCCGGAGCAGGGGCTGCTCGGCCTGCGCAAGGGCCTGGGCGTCTTCGCGAACCTGCGCCC
GGTGCGCGTGCACCCGAGCCTGCTCGACTCCTCCCCGCTCCGCCCGGAGAAGCTCCGCGGCGTGGACATCATGGTGATCC
GCGAGCTGACCGGCGGCCTCTACTTCGGCCAGCCCAAGGGGCGCGACGTGAAGGACGGGCACGCGCGCGCGGTGGACACG
CTCGAGTACCAGGACTTCGAGGTGCGCCGGGTGGTGGAGCTCGCGTTCCGCATCGCCAAGGGGCGGAAGAAGAAGGTCAC
GAGCGTGGACAAGGCGAACGTGCTCGAGTCCTCGCGGCTCTGGCGCGAGATCGCGACCGCCATCGGGCAGGCGAACCCCG
ACGTGGCGCTCGATCACATGCTGGTGGACACCGCCGCCATGCGGCTCGTCACCAGCCCGGCCACGCTCGACGTGGTCGTC
ACCGAGAACATGTTCGGCGACATCCTCACCGACGAGGCCTCGGTGCTGGCCGGCTCGATGGGCATGCTCCCGTCCGCCTC
CATCGGCGAGAAGGGGCCGGGCCTCTACGAGCCCATCCACGGCTCCGCCCCGGACATCGCCGGCAAGGGCATCGCGAACC
CGGTGGGCACCGTGCTCTCCGCCGCGCTGCTGCTCCGCCACTCGCTCGGCCTCGAGCCGGAGGCGGCCGCCATCGAGCGC
GCCGTGGACCAGGCCATCACCGACGGCTGCCGCACCGCCGACCTGGGCGGGAAGCTCTCCACCCGCGCCATGGCCGACGA
GATCCTGAAGCGGCTCGCCTAG

Upstream 100 bases:

>100_bases
GAACATCATCGAGGCGAGCTGGATCGCGCTGGTGGACGCGGTCGAGTACGGGCTCGCCCTCGCCTAGACCTCACACCAGG
AACCCAAGGAGACACCGAGC

Downstream 100 bases:

>100_bases
CCACGCCCCGGGACGCCGCCATGCCGGCCGGCCCACGCGGCCCGCTCCCCGCGCTGGACGGGCTGCGCGGGCTGGCCATC
GGCGGCGTCCTCGCCTGCCA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNAKIVVLPGDGIGPEVTAEAVRVLEAVARRGGHTLSFTERLMGGCSIDAHGTALTPEVLADCQAADAVLLGAVGGPKWD
DPRARVRPEQGLLGLRKGLGVFANLRPVRVHPSLLDSSPLRPEKLRGVDIMVIRELTGGLYFGQPKGRDVKDGHARAVDT
LEYQDFEVRRVVELAFRIAKGRKKKVTSVDKANVLESSRLWREIATAIGQANPDVALDHMLVDTAAMRLVTSPATLDVVV
TENMFGDILTDEASVLAGSMGMLPSASIGEKGPGLYEPIHGSAPDIAGKGIANPVGTVLSAALLLRHSLGLEPEAAAIER
AVDQAITDGCRTADLGGKLSTRAMADEILKRLA

Sequences:

>Translated_353_residues
MNAKIVVLPGDGIGPEVTAEAVRVLEAVARRGGHTLSFTERLMGGCSIDAHGTALTPEVLADCQAADAVLLGAVGGPKWD
DPRARVRPEQGLLGLRKGLGVFANLRPVRVHPSLLDSSPLRPEKLRGVDIMVIRELTGGLYFGQPKGRDVKDGHARAVDT
LEYQDFEVRRVVELAFRIAKGRKKKVTSVDKANVLESSRLWREIATAIGQANPDVALDHMLVDTAAMRLVTSPATLDVVV
TENMFGDILTDEASVLAGSMGMLPSASIGEKGPGLYEPIHGSAPDIAGKGIANPVGTVLSAALLLRHSLGLEPEAAAIER
AVDQAITDGCRTADLGGKLSTRAMADEILKRLA
>Mature_353_residues
MNAKIVVLPGDGIGPEVTAEAVRVLEAVARRGGHTLSFTERLMGGCSIDAHGTALTPEVLADCQAADAVLLGAVGGPKWD
DPRARVRPEQGLLGLRKGLGVFANLRPVRVHPSLLDSSPLRPEKLRGVDIMVIRELTGGLYFGQPKGRDVKDGHARAVDT
LEYQDFEVRRVVELAFRIAKGRKKKVTSVDKANVLESSRLWREIATAIGQANPDVALDHMLVDTAAMRLVTSPATLDVVV
TENMFGDILTDEASVLAGSMGMLPSASIGEKGPGLYEPIHGSAPDIAGKGIANPVGTVLSAALLLRHSLGLEPEAAAIER
AVDQAITDGCRTADLGGKLSTRAMADEILKRLA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=351, Percent_Identity=32.7635327635328, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI4758582, Length=352, Percent_Identity=25.5681818181818, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI28178816, Length=357, Percent_Identity=26.890756302521, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI28178821, Length=357, Percent_Identity=26.890756302521, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI28178838, Length=321, Percent_Identity=26.4797507788162, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28178819, Length=188, Percent_Identity=30.3191489361702, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=50.7002801120448, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=35.0974930362117, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1787381, Length=383, Percent_Identity=25.065274151436, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=356, Percent_Identity=32.0224719101124, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=359, Percent_Identity=30.6406685236769, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17550882, Length=361, Percent_Identity=28.2548476454294, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=351, Percent_Identity=27.3504273504274, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=48.2191780821918, Blast_Score=300, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6322097, Length=371, Percent_Identity=36.1185983827493, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=30.8988764044944, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324291, Length=357, Percent_Identity=30.2521008403361, Blast_Score=126, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24643268, Length=363, Percent_Identity=32.7823691460055, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24643270, Length=363, Percent_Identity=32.7823691460055, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24661184, Length=361, Percent_Identity=31.8559556786704, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161078639, Length=265, Percent_Identity=29.0566037735849, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078633, Length=265, Percent_Identity=29.0566037735849, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24650122, Length=265, Percent_Identity=29.0566037735849, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078635, Length=265, Percent_Identity=29.0566037735849, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078637, Length=265, Percent_Identity=29.0566037735849, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=27.5568181818182, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=27.5568181818182, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI20130355, Length=173, Percent_Identity=30.0578034682081, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37315; Mature: 37315

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKIVVLPGDGIGPEVTAEAVRVLEAVARRGGHTLSFTERLMGGCSIDAHGTALTPEVL
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCCCCCCCCHHHH
ADCQAADAVLLGAVGGPKWDDPRARVRPEQGLLGLRKGLGVFANLRPVRVHPSLLDSSPL
HHHHHHCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCEEEECHHHHCCCCC
RPEKLRGVDIMVIRELTGGLYFGQPKGRDVKDGHARAVDTLEYQDFEVRRVVELAFRIAK
CHHHHCCCEEEEEEEHHCCEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHC
GRKKKVTSVDKANVLESSRLWREIATAIGQANPDVALDHMLVDTAAMRLVTSPATLDVVV
CCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
TENMFGDILTDEASVLAGSMGMLPSASIGEKGPGLYEPIHGSAPDIAGKGIANPVGTVLS
ECCHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AALLLRHSLGLEPEAAAIERAVDQAITDGCRTADLGGKLSTRAMADEILKRLA
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAKIVVLPGDGIGPEVTAEAVRVLEAVARRGGHTLSFTERLMGGCSIDAHGTALTPEVL
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCCCCCCCCHHHH
ADCQAADAVLLGAVGGPKWDDPRARVRPEQGLLGLRKGLGVFANLRPVRVHPSLLDSSPL
HHHHHHCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCEEEECHHHHCCCCC
RPEKLRGVDIMVIRELTGGLYFGQPKGRDVKDGHARAVDTLEYQDFEVRRVVELAFRIAK
CHHHHCCCEEEEEEEHHCCEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHC
GRKKKVTSVDKANVLESSRLWREIATAIGQANPDVALDHMLVDTAAMRLVTSPATLDVVV
CCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
TENMFGDILTDEASVLAGSMGMLPSASIGEKGPGLYEPIHGSAPDIAGKGIANPVGTVLS
ECCHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AALLLRHSLGLEPEAAAIERAVDQAITDGCRTADLGGKLSTRAMADEILKRLA
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA