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The map label for this gene is hybA [C]

Identifier: 220916973

GI number: 220916973

Start: 2099444

End: 2101639

Strand: Direct

Name: hybA [C]

Synonym: A2cp1_1869

Alternate gene names: 220916973

Gene position: 2099444-2101639 (Clockwise)

Preceding gene: 220916972

Following gene: 220916974

Centisome position: 41.74

GC content: 70.63

Gene sequence:

>2196_bases
ATGAGCAAGGCCACGTTGATCGACACGACGTACTGCATCGGGTGCCGCTCCTGCCAGAGCACCTGCAAGCAATGGAACGA
CCTCCCCGCCGAGCAGACCGTCCTGCTCGGCGGTGACAAGGGGCTCCAGAACCCCAACACGCTGACCTCCAGCACGTTCG
CGGTGGTCACGTTCGACGAGGTGGAGGACGCGAGCGCGCCGGGCGGGCTCCGGTACGTCTCCACCAAGCGCCAGTGCATG
CACTGCGAGGAGCCGGCCTGCGCGGCCGCCTGCCCGGTGACGGCGCTGCACAAGACCGAGTCGGGCGCGGTCGCCTACGA
CGCCTCGAAGTGCATCGGCTGCCGCTACTGCATGTGGGCCTGCCCGTTCGGCGTGCCCACCGCGGAGTGGGACTCGCTCG
CGCCGAAGATCCAGAAGTGCGACATGTGCGTGGGCCGCCAGACGGCGGCCGTGCCCGTGGAGCGCAACGGCGTCGCGCTC
ACCGCCGAGGAGCGCACCCACCTCGCCGCGGCGTACGCGATCCCCGCCTGCGTGAAGCAGTGCCCGGCCGGCGCGCTGAA
GTACGGCGACCGGGACGAGCTGCTGAAGGAGGCGCACGCGCGCATCGCCGCCAGCCCGTCCAAGTACGTGGACCACGTCT
ACGGCGAGCACGAGGTGGGCGGGACCAACATGCTGTACCTGTCGCCGGTCCCGTTCGAGAAGCTCGGGTTCCCCATGGAC
CTCGGCACCGATCCGCTCCCGCGCCGCAGCGCGGTGGCGCTCGGGGCGGTCCCGCCCGCGGTCATCGGGGTGGGCGCGGC
GCTCGGCGGCGTGTACGCGCTGTCGAAGCGCAAGCAGGAGGTGAAGGCGAAGGAGGGGAAGGCGCACGAGCACCACCCCG
AGTTCGCCCCGGTGAAGCAGCCGTTCTGGACCACCGCGAACAAGCTGCTCGCGGCGGTGATGGCCTGGGGCGCGATCTCC
TTCGTGGCCCGGTTCGCCCTCGGCCTGGGCGGCTCCACCAACCTGAGCGACACCTACGCCTGGGGCCTCTGGATCGTCTT
CGACCTGGTGTGGATCGCGGTCGCGGCCGGCGCGTTCGCCACCGCGGGCCTCATCTACGTGCTCCAGCGGAAGGACCTGT
ACTCCATCGGACGGTCCGCCGTACTGATGGGCCTGCTGTCCTACTCCTTCGTCACGGTGACGCTGCTCGCCGACCTCGGC
CTGCCGTGGCACTTCTGGCGCCTCGGCACCGAGGCCCCGCACCACTCGGCCATGTTCGAGGTCTCCTGGTGCGTCGGCCT
GTACGTGACGGTCCTCGCGTTCGAGTTCATGCCGGTGCCGTTCGAGCGCTGGGGCATGAAGAAGGCGATGGACGCCTGGA
AGCGCTGGTCGCCCTGGTACGTGGTGGGCGCGGTGACGCTGTTCGTCTACCTGATGTCCCGCAACGTGCTGATCGCCGCC
GGCGCGGCGGCGGTGTTCTCGGTCCTGGCGTACGCGTTCCGCACGCGCCCGGGCGAGAAGCCGGTGCCCATCCTGCTCGC
CATCGCGGCGGTGACGCTGTCCACCATGCACCAGAGCTCGCTGGGCTCGCTGTTCCTGCTCATGCCCGACAAGCTCGACC
ACGCCTGGTGGTCGCCGGTCATGCCGGTCTACTTCTTCCTCTCGTCCGTCGCGGCTGGTCTGGGCCTGATGGTGCTGGTG
GAGCTGTGGATCGCGAAGGCGTTCAAGCGGCAGGTCCGCGTGGCGCAGCTCGCCGCGCTGGGCAAGGTGGCGTTCTGGGC
CCTCGCGGTGTACGAGGCGTTCCGCCTGGGCGACCTGGCCGTCCGCGGCCAGCTCGGGCACGCGTTCACCGGCCCGAAGG
CCGGCCTGTTCCTGGTCGAGGTGCTGCTGGGCGGCCTGCTCCCGCTCGTGCTCCTCGGCGCGGCGAAGCTCCGTGAGCGC
CCGGCTGTCCTGGGCCTGGCGTCGGCCCTCGCGACCGGCGGCATCGTGCTGAACCGCATGAGCGTGGTGGTGTTCGCGAT
GAACCTGAAGGGCGCCATGCCCCAGGACTCGGCGCAGCCGTACCTGCCGAGCGCGGTCGAGTGGGGCGTCTCGCTCGGCC
TCATCGCGGCGACCATCTTCCTGTTCGGCCTCGCCGTCCGTCACATGCCGGTGCTGCCGAAGGAGGAGCCCGCCCAGGCG
GCGAACGAGCCGAACGCAGAGCAGGCCAGCGCGTAA

Upstream 100 bases:

>100_bases
GAGCCGGCACCCAGGAGACGAAGGCGTGCCTCGTCGACGTCGCTCGCGTGGAGGAGGCCGTCGCCTCGCGGTAGGGGCGG
CGCGCTGAAAGGACACGACG

Downstream 100 bases:

>100_bases
CGCGCCAGCGCGTACGCGACCCCGAAGCTCCCGGTGCCGGTTCCCGGTGCCGGGAGCTTCTCTTTTCGCTACAAGGGAAG
GCCCATGGACACGGAACTCC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; NADH

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MSKATLIDTTYCIGCRSCQSTCKQWNDLPAEQTVLLGGDKGLQNPNTLTSSTFAVVTFDEVEDASAPGGLRYVSTKRQCM
HCEEPACAAACPVTALHKTESGAVAYDASKCIGCRYCMWACPFGVPTAEWDSLAPKIQKCDMCVGRQTAAVPVERNGVAL
TAEERTHLAAAYAIPACVKQCPAGALKYGDRDELLKEAHARIAASPSKYVDHVYGEHEVGGTNMLYLSPVPFEKLGFPMD
LGTDPLPRRSAVALGAVPPAVIGVGAALGGVYALSKRKQEVKAKEGKAHEHHPEFAPVKQPFWTTANKLLAAVMAWGAIS
FVARFALGLGGSTNLSDTYAWGLWIVFDLVWIAVAAGAFATAGLIYVLQRKDLYSIGRSAVLMGLLSYSFVTVTLLADLG
LPWHFWRLGTEAPHHSAMFEVSWCVGLYVTVLAFEFMPVPFERWGMKKAMDAWKRWSPWYVVGAVTLFVYLMSRNVLIAA
GAAAVFSVLAYAFRTRPGEKPVPILLAIAAVTLSTMHQSSLGSLFLLMPDKLDHAWWSPVMPVYFFLSSVAAGLGLMVLV
ELWIAKAFKRQVRVAQLAALGKVAFWALAVYEAFRLGDLAVRGQLGHAFTGPKAGLFLVEVLLGGLLPLVLLGAAKLRER
PAVLGLASALATGGIVLNRMSVVVFAMNLKGAMPQDSAQPYLPSAVEWGVSLGLIAATIFLFGLAVRHMPVLPKEEPAQA
ANEPNAEQASA

Sequences:

>Translated_731_residues
MSKATLIDTTYCIGCRSCQSTCKQWNDLPAEQTVLLGGDKGLQNPNTLTSSTFAVVTFDEVEDASAPGGLRYVSTKRQCM
HCEEPACAAACPVTALHKTESGAVAYDASKCIGCRYCMWACPFGVPTAEWDSLAPKIQKCDMCVGRQTAAVPVERNGVAL
TAEERTHLAAAYAIPACVKQCPAGALKYGDRDELLKEAHARIAASPSKYVDHVYGEHEVGGTNMLYLSPVPFEKLGFPMD
LGTDPLPRRSAVALGAVPPAVIGVGAALGGVYALSKRKQEVKAKEGKAHEHHPEFAPVKQPFWTTANKLLAAVMAWGAIS
FVARFALGLGGSTNLSDTYAWGLWIVFDLVWIAVAAGAFATAGLIYVLQRKDLYSIGRSAVLMGLLSYSFVTVTLLADLG
LPWHFWRLGTEAPHHSAMFEVSWCVGLYVTVLAFEFMPVPFERWGMKKAMDAWKRWSPWYVVGAVTLFVYLMSRNVLIAA
GAAAVFSVLAYAFRTRPGEKPVPILLAIAAVTLSTMHQSSLGSLFLLMPDKLDHAWWSPVMPVYFFLSSVAAGLGLMVLV
ELWIAKAFKRQVRVAQLAALGKVAFWALAVYEAFRLGDLAVRGQLGHAFTGPKAGLFLVEVLLGGLLPLVLLGAAKLRER
PAVLGLASALATGGIVLNRMSVVVFAMNLKGAMPQDSAQPYLPSAVEWGVSLGLIAATIFLFGLAVRHMPVLPKEEPAQA
ANEPNAEQASA
>Mature_730_residues
SKATLIDTTYCIGCRSCQSTCKQWNDLPAEQTVLLGGDKGLQNPNTLTSSTFAVVTFDEVEDASAPGGLRYVSTKRQCMH
CEEPACAAACPVTALHKTESGAVAYDASKCIGCRYCMWACPFGVPTAEWDSLAPKIQKCDMCVGRQTAAVPVERNGVALT
AEERTHLAAAYAIPACVKQCPAGALKYGDRDELLKEAHARIAASPSKYVDHVYGEHEVGGTNMLYLSPVPFEKLGFPMDL
GTDPLPRRSAVALGAVPPAVIGVGAALGGVYALSKRKQEVKAKEGKAHEHHPEFAPVKQPFWTTANKLLAAVMAWGAISF
VARFALGLGGSTNLSDTYAWGLWIVFDLVWIAVAAGAFATAGLIYVLQRKDLYSIGRSAVLMGLLSYSFVTVTLLADLGL
PWHFWRLGTEAPHHSAMFEVSWCVGLYVTVLAFEFMPVPFERWGMKKAMDAWKRWSPWYVVGAVTLFVYLMSRNVLIAAG
AAAVFSVLAYAFRTRPGEKPVPILLAIAAVTLSTMHQSSLGSLFLLMPDKLDHAWWSPVMPVYFFLSSVAAGLGLMVLVE
LWIAKAFKRQVRVAQLAALGKVAFWALAVYEAFRLGDLAVRGQLGHAFTGPKAGLFLVEVLLGGLLPLVLLGAAKLRERP
AVLGLASALATGGIVLNRMSVVVFAMNLKGAMPQDSAQPYLPSAVEWGVSLGLIAATIFLFGLAVRHMPVLPKEEPAQAA
NEPNAEQASA

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=266, Percent_Identity=33.4586466165414, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1787749, Length=299, Percent_Identity=33.1103678929766, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI1790326, Length=228, Percent_Identity=33.7719298245614, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI2367183, Length=416, Percent_Identity=27.4038461538462, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1787122, Length=204, Percent_Identity=33.8235294117647, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1787872, Length=204, Percent_Identity=32.3529411764706, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI226510944, Length=189, Percent_Identity=29.6296296296296, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI2367245, Length=205, Percent_Identity=25.8536585365854, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI87082179, Length=207, Percent_Identity=28.9855072463768, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI2367345, Length=201, Percent_Identity=25.3731343283582, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789079, Length=207, Percent_Identity=28.0193236714976, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788811, Length=194, Percent_Identity=27.319587628866, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87082180, Length=123, Percent_Identity=34.9593495934959, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: 1.2.1.2

Molecular weight: Translated: 78580; Mature: 78449

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKATLIDTTYCIGCRSCQSTCKQWNDLPAEQTVLLGGDKGLQNPNTLTSSTFAVVTFDE
CCCCEEEECHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEECC
VEDASAPGGLRYVSTKRQCMHCEEPACAAACPVTALHKTESGAVAYDASKCIGCRYCMWA
CCCCCCCCCEEEHHHHHHHHCCCCCCHHHHCCHHHHHCCCCCCEEECHHHHHHHHHHHHH
CPFGVPTAEWDSLAPKIQKCDMCVGRQTAAVPVERNGVALTAEERTHLAAAYAIPACVKQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEECEEECCCEEEEECCHHHHHHHHHHHHHHHH
CPAGALKYGDRDELLKEAHARIAASPSKYVDHVYGEHEVGGTNMLYLSPVPFEKLGFPMD
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHCCCCCC
LGTDPLPRRSAVALGAVPPAVIGVGAALGGVYALSKRKQEVKAKEGKAHEHHPEFAPVKQ
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFWTTANKLLAAVMAWGAISFVARFALGLGGSTNLSDTYAWGLWIVFDLVWIAVAAGAFA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAGLIYVLQRKDLYSIGRSAVLMGLLSYSFVTVTLLADLGLPWHFWRLGTEAPHHSAMFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHH
VSWCVGLYVTVLAFEFMPVPFERWGMKKAMDAWKRWSPWYVVGAVTLFVYLMSRNVLIAA
HHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEE
GAAAVFSVLAYAFRTRPGEKPVPILLAIAAVTLSTMHQSSLGSLFLLMPDKLDHAWWSPV
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHCHHHCCHH
MPVYFFLSSVAAGLGLMVLVELWIAKAFKRQVRVAQLAALGKVAFWALAVYEAFRLGDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VRGQLGHAFTGPKAGLFLVEVLLGGLLPLVLLGAAKLRERPAVLGLASALATGGIVLNRM
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHH
SVVVFAMNLKGAMPQDSAQPYLPSAVEWGVSLGLIAATIFLFGLAVRHMPVLPKEEPAQA
HHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
ANEPNAEQASA
CCCCCCCCCCC
>Mature Secondary Structure 
SKATLIDTTYCIGCRSCQSTCKQWNDLPAEQTVLLGGDKGLQNPNTLTSSTFAVVTFDE
CCCEEEECHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEECC
VEDASAPGGLRYVSTKRQCMHCEEPACAAACPVTALHKTESGAVAYDASKCIGCRYCMWA
CCCCCCCCCEEEHHHHHHHHCCCCCCHHHHCCHHHHHCCCCCCEEECHHHHHHHHHHHHH
CPFGVPTAEWDSLAPKIQKCDMCVGRQTAAVPVERNGVALTAEERTHLAAAYAIPACVKQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEECEEECCCEEEEECCHHHHHHHHHHHHHHHH
CPAGALKYGDRDELLKEAHARIAASPSKYVDHVYGEHEVGGTNMLYLSPVPFEKLGFPMD
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHCCCCCC
LGTDPLPRRSAVALGAVPPAVIGVGAALGGVYALSKRKQEVKAKEGKAHEHHPEFAPVKQ
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFWTTANKLLAAVMAWGAISFVARFALGLGGSTNLSDTYAWGLWIVFDLVWIAVAAGAFA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAGLIYVLQRKDLYSIGRSAVLMGLLSYSFVTVTLLADLGLPWHFWRLGTEAPHHSAMFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHH
VSWCVGLYVTVLAFEFMPVPFERWGMKKAMDAWKRWSPWYVVGAVTLFVYLMSRNVLIAA
HHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEE
GAAAVFSVLAYAFRTRPGEKPVPILLAIAAVTLSTMHQSSLGSLFLLMPDKLDHAWWSPV
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHCHHHCCHH
MPVYFFLSSVAAGLGLMVLVELWIAKAFKRQVRVAQLAALGKVAFWALAVYEAFRLGDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VRGQLGHAFTGPKAGLFLVEVLLGGLLPLVLLGAAKLRERPAVLGLASALATGGIVLNRM
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHH
SVVVFAMNLKGAMPQDSAQPYLPSAVEWGVSLGLIAATIFLFGLAVRHMPVLPKEEPAQA
HHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
ANEPNAEQASA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]