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The map label for this gene is nei [C]

Identifier: 220916970

GI number: 220916970

Start: 2092744

End: 2093580

Strand: Direct

Name: nei [C]

Synonym: A2cp1_1866

Alternate gene names: 220916970

Gene position: 2092744-2093580 (Clockwise)

Preceding gene: 220916969

Following gene: 220916972

Centisome position: 41.61

GC content: 79.09

Gene sequence:

>837_bases
ATGCCGGAGGGCGACACGCTGGCCCGGGCCGCGCGCGCCCTGCACCGCGCGCTCGCCGGCAAGCCGGTGGTCCGCTTCGA
GACCGTGCTGCCCCGGCTGGCACGCGTGGCCGCCGACGCGCCGGTGGTCGGCCGGACCGTGGAGCGGGCCGAGGCCGTCG
GCAAGCACCTGCTCCTCCACCTCTCCGGCGGCCTGGTGCTCCGCACGCACTTGCGCATGAACGGGAGCTGGCACCTGTAC
CGGCCCGGCGCCCCCTGGCGGCGCCCGGCCTCGGCGATGCGCGTGCTGCTGGAGGTGCCGGACGCGGTGGCGGTGGCGTT
CGATCTCCCGGTCGCGGAGTGGCTCCGCGCGGCGGACCTGGGGCGCCACCGGGCCCTGTCACGCCTCGGGCCCGACCTGC
TCTCCCCCGCGTTCGACGCGGCCGAGGCGGAGCGGCGGCTGCGGGCGCGCGGGGGGCTCGCGGTGGCGGACGCGCTGGTG
GACCAGGCGGCGCTCGCCGGCGCCGGGAACGAGCTCAAGTCGGAGATCCTGTTCGTGGCCGGGGTGAGCCCGTTCCGGCG
GGTCGAGGACCTCGACGACGGCGAGCTCCGGGCGGTGATCGCGACGGCGCGGCGGCTGATCGGCGAGAACGTGCCGCCGC
CCGGGCCGGGTGGGGTGGAGACCTGGCGCGGCGGACGGCGCACCACCCGCCGCATGAACCCGCGGGAGCGGACCTGGGTG
TACGGCCGGGGCGGGCGCCCGTGCCGCCGGTGCGGCGCGCCCATCGCGTTCGCGCGCCAGGGCCCGCACGCCCAGGGCAC
CTGGTGGTGTCCGCGGTGCCAGCCGGGGCCGGCGTAG

Upstream 100 bases:

>100_bases
TCCTGGTGCAGGCCGGGTTCTCGCCGGCGGGGACCGCGCTGCAGCTCCCGCGCCGCGCCGCCGGCGCGCTCACGCTCCCG
TCCGGCGCCGGGCTGGCCTG

Downstream 100 bases:

>100_bases
CGCGGTCAGGCGGTGGGGGGCGGGCTGGTGGTGGCGGCGCCGGCCCGGTGCGTCGCGGCGGCCTCGGGCGCGCGCGACAG
CGTCACGGTGAAGGTCGAGC

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase SCO5760; Putative AP lyase SCO5760 [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MPEGDTLARAARALHRALAGKPVVRFETVLPRLARVAADAPVVGRTVERAEAVGKHLLLHLSGGLVLRTHLRMNGSWHLY
RPGAPWRRPASAMRVLLEVPDAVAVAFDLPVAEWLRAADLGRHRALSRLGPDLLSPAFDAAEAERRLRARGGLAVADALV
DQAALAGAGNELKSEILFVAGVSPFRRVEDLDDGELRAVIATARRLIGENVPPPGPGGVETWRGGRRTTRRMNPRERTWV
YGRGGRPCRRCGAPIAFARQGPHAQGTWWCPRCQPGPA

Sequences:

>Translated_278_residues
MPEGDTLARAARALHRALAGKPVVRFETVLPRLARVAADAPVVGRTVERAEAVGKHLLLHLSGGLVLRTHLRMNGSWHLY
RPGAPWRRPASAMRVLLEVPDAVAVAFDLPVAEWLRAADLGRHRALSRLGPDLLSPAFDAAEAERRLRARGGLAVADALV
DQAALAGAGNELKSEILFVAGVSPFRRVEDLDDGELRAVIATARRLIGENVPPPGPGGVETWRGGRRTTRRMNPRERTWV
YGRGGRPCRRCGAPIAFARQGPHAQGTWWCPRCQPGPA
>Mature_277_residues
PEGDTLARAARALHRALAGKPVVRFETVLPRLARVAADAPVVGRTVERAEAVGKHLLLHLSGGLVLRTHLRMNGSWHLYR
PGAPWRRPASAMRVLLEVPDAVAVAFDLPVAEWLRAADLGRHRALSRLGPDLLSPAFDAAEAERRLRARGGLAVADALVD
QAALAGAGNELKSEILFVAGVSPFRRVEDLDDGELRAVIATARRLIGENVPPPGPGGVETWRGGRRTTRRMNPRERTWVY
GRGGRPCRRCGAPIAFARQGPHAQGTWWCPRCQPGPA

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786932, Length=277, Percent_Identity=30.6859205776173, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790066, Length=240, Percent_Identity=29.1666666666667, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 30153; Mature: 30022

Theoretical pI: Translated: 11.77; Mature: 11.77

Prosite motif: PS51066 ZF_FPG_2 ; PS51068 FPG_CAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGDTLARAARALHRALAGKPVVRFETVLPRLARVAADAPVVGRTVERAEAVGKHLLLH
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEHH
LSGGLVLRTHLRMNGSWHLYRPGAPWRRPASAMRVLLEVPDAVAVAFDLPVAEWLRAADL
CCCCEEEEEEEEECCCEEEECCCCCCCCCHHHHHHHHHCCCHHEEEECCCHHHHHHHHHH
GRHRALSRLGPDLLSPAFDAAEAERRLRARGGLAVADALVDQAALAGAGNELKSEILFVA
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHEEEEE
GVSPFRRVEDLDDGELRAVIATARRLIGENVPPPGPGGVETWRGGRRTTRRMNPRERTWV
CCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEE
YGRGGRPCRRCGAPIAFARQGPHAQGTWWCPRCQPGPA
ECCCCCHHHHHCCCEEECCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure 
PEGDTLARAARALHRALAGKPVVRFETVLPRLARVAADAPVVGRTVERAEAVGKHLLLH
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEHH
LSGGLVLRTHLRMNGSWHLYRPGAPWRRPASAMRVLLEVPDAVAVAFDLPVAEWLRAADL
CCCCEEEEEEEEECCCEEEECCCCCCCCCHHHHHHHHHCCCHHEEEECCCHHHHHHHHHH
GRHRALSRLGPDLLSPAFDAAEAERRLRARGGLAVADALVDQAALAGAGNELKSEILFVA
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHEEEEE
GVSPFRRVEDLDDGELRAVIATARRLIGENVPPPGPGGVETWRGGRRTTRRMNPRERTWV
CCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEE
YGRGGRPCRRCGAPIAFARQGPHAQGTWWCPRCQPGPA
ECCCCCHHHHHCCCEEECCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]