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The map label for this gene is ushA [H]

Identifier: 220916777

GI number: 220916777

Start: 1863579

End: 1865294

Strand: Direct

Name: ushA [H]

Synonym: A2cp1_1672

Alternate gene names: 220916777

Gene position: 1863579-1865294 (Clockwise)

Preceding gene: 220916776

Following gene: 220916778

Centisome position: 37.05

GC content: 72.79

Gene sequence:

>1716_bases
ATGGATCGCAGGGAGTTCATGCGGATGCTGGCGGCGGCCGCCGCCGGCGGGATGACCCTTCGCGCGCGCGCGGCGGGTCC
GGAGGGCGCGGGGCTGTACGACCTGCCGCGCCACGGCAACGTGAGCCTGCTGCACTTCACCGACGTGCACGCGCAGCTCA
TGCCGCTCCACTACCGGGAGCCGGCGGTGAACCTGGGCGCCGGGCCGGCCCGGGGCCGCCCGCCGCACCTGGTCGGGAAG
GCGCTCCTCTCGCACTTCGGGATCCGGCCCGGGACGCGCGAGGCGCACGCGCTCACGTTCCTCGACTTCGAGGCGGCGGC
GCGGCGGTACGGCAAGGTCGGCGGCTTCGCGCACCTCGCCACGCTGGTGAAGCGCCTCCGGGCCAGCCGCCCCGGCGCGC
TGCTGCTCGACGGCGGCGACACCTGGCAGGGCTCGGCCACGGCGCTGTGGACCGACGGCCAGGACATGATCGACGCGGCC
AAGCTGCTCGGCGTGGACGTGATGACCGGCCACTGGGAGTTCACGCTCGGCGCCGACCGGGTGAAGCGCGCGGTCGAGAA
GGACCTCGCCGGCAAGATCTCGTTCGTCGCCCAGAACGTGCACACCGCCGACTTCGGCGACCCGGTGTTCGAGCCCTGGG
TGATGCGCGAGGTGAACGGCGTACCGGTGGCCGTGATCGGGCAGGCGTTCCCGTACACCCCCATCGCGAACCCGCGGTAC
CTCGTGCCCGACTGGACGTTCGGGATCGACGAGGAGAACCTCCAGCGCACCGTGGACGAGGCGCGGGCGAAGGGCGCGCG
CGTGGTGGTGCTGCTCTCGCACGACGGCATGGACGTGGACCTGAAGCTCGCGTCCCGCGTGAGCGGCATCGACGCGATCC
TGGGCGGCCACACGCACGACGGCGTCCCGCGCCCGGTCGCGGTGAAGAACCGCTCCGGCCAGACGCTGGTCACGAACGCG
GGCTCGAACGGCAAGTTCCTGGGCGTGCTCGATCTGGACGTCCGCGGTGGGAAGGTGGCGAGCCACCGCTACCGGCTGCT
CCCGGTGTTCTCGAACCTGCTCCCGGCCGACCCGGAGATGGCCGCGCACATCGAGCGCGTCCGCGCGCCGTTCCGCGAGA
AGCTCGCCGCCCCGCTGGCGGTCACCGAGGGCGTGCTCTACCGGCGCGGCAACTTCAACGGAACGTACGATCAGCTCATC
CTGGACGCGCTCATGGCCGAGAAGGACGCCGAGATCGCGTTCTCGCCCGGCTTCCGCTGGGGCGCCTCGCTCCTGCCCGG
CGACACCATCACGCAGGAGGACCTGCTCGCGGCCACCGCCATCAGCTACCCGAACGTCGGCGTCAACGACATGACCGGCG
CGACGGTGAGGGCGGTGCTCGAGGACGTCTGCGACAACATCTTCAACCCGGACCCCTACTACCAGCAGGGCGGCGACATG
GTCCGGGTCGGCGGGCTCGAGTACGCGTGCCACCCCGGCGCGCGGAGCGGGGAGCGCATCCAGGACCTGCGCCTGCGCGG
GAAGCCGCTGGAGGCGTCGAAGACGTACCGCGTCGCCACCTGGGCCTCGGTGAGCGAGGCGGCGCGCGACGCCGGCGGCG
AGCCGGTCTGGGACGTGGTGGGCCGCTGGCTGCGCGCCCAGAAGACGATCACGCCGCGCACGCCGAACCTGCCGCGCCTG
GTCGGCGTGGGCCGGGATCCGGGGATGTCCACCTGA

Upstream 100 bases:

>100_bases
GCACCACGCCATCCTCACCGAGGAGCAGATCAGGGATCTGGTCGCGCTGCTCCTCGATCCCGCCTCCCCGGTGAACCAGT
AGCGCGGAGCGAGCGAGCCC

Downstream 100 bases:

>100_bases
TGGCGCCCGCGCTCGCCACGCTGCTCGCGGCGCTCGCCGCGGCCGCCCCCGCGCCGGCCCGCGCGGCCGATCCCGTCCTC
GAGCCGGTCCGGCTCGCGCC

Product: 5'-Nucleotidase domain-containing protein

Products: NA

Alternate protein names: UDP-sugar hydrolase; UDP-sugar diphosphatase; UDP-sugar pyrophosphatase; 5'-nucleotidase; 5'-NT [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MDRREFMRMLAAAAAGGMTLRARAAGPEGAGLYDLPRHGNVSLLHFTDVHAQLMPLHYREPAVNLGAGPARGRPPHLVGK
ALLSHFGIRPGTREAHALTFLDFEAAARRYGKVGGFAHLATLVKRLRASRPGALLLDGGDTWQGSATALWTDGQDMIDAA
KLLGVDVMTGHWEFTLGADRVKRAVEKDLAGKISFVAQNVHTADFGDPVFEPWVMREVNGVPVAVIGQAFPYTPIANPRY
LVPDWTFGIDEENLQRTVDEARAKGARVVVLLSHDGMDVDLKLASRVSGIDAILGGHTHDGVPRPVAVKNRSGQTLVTNA
GSNGKFLGVLDLDVRGGKVASHRYRLLPVFSNLLPADPEMAAHIERVRAPFREKLAAPLAVTEGVLYRRGNFNGTYDQLI
LDALMAEKDAEIAFSPGFRWGASLLPGDTITQEDLLAATAISYPNVGVNDMTGATVRAVLEDVCDNIFNPDPYYQQGGDM
VRVGGLEYACHPGARSGERIQDLRLRGKPLEASKTYRVATWASVSEAARDAGGEPVWDVVGRWLRAQKTITPRTPNLPRL
VGVGRDPGMST

Sequences:

>Translated_571_residues
MDRREFMRMLAAAAAGGMTLRARAAGPEGAGLYDLPRHGNVSLLHFTDVHAQLMPLHYREPAVNLGAGPARGRPPHLVGK
ALLSHFGIRPGTREAHALTFLDFEAAARRYGKVGGFAHLATLVKRLRASRPGALLLDGGDTWQGSATALWTDGQDMIDAA
KLLGVDVMTGHWEFTLGADRVKRAVEKDLAGKISFVAQNVHTADFGDPVFEPWVMREVNGVPVAVIGQAFPYTPIANPRY
LVPDWTFGIDEENLQRTVDEARAKGARVVVLLSHDGMDVDLKLASRVSGIDAILGGHTHDGVPRPVAVKNRSGQTLVTNA
GSNGKFLGVLDLDVRGGKVASHRYRLLPVFSNLLPADPEMAAHIERVRAPFREKLAAPLAVTEGVLYRRGNFNGTYDQLI
LDALMAEKDAEIAFSPGFRWGASLLPGDTITQEDLLAATAISYPNVGVNDMTGATVRAVLEDVCDNIFNPDPYYQQGGDM
VRVGGLEYACHPGARSGERIQDLRLRGKPLEASKTYRVATWASVSEAARDAGGEPVWDVVGRWLRAQKTITPRTPNLPRL
VGVGRDPGMST
>Mature_571_residues
MDRREFMRMLAAAAAGGMTLRARAAGPEGAGLYDLPRHGNVSLLHFTDVHAQLMPLHYREPAVNLGAGPARGRPPHLVGK
ALLSHFGIRPGTREAHALTFLDFEAAARRYGKVGGFAHLATLVKRLRASRPGALLLDGGDTWQGSATALWTDGQDMIDAA
KLLGVDVMTGHWEFTLGADRVKRAVEKDLAGKISFVAQNVHTADFGDPVFEPWVMREVNGVPVAVIGQAFPYTPIANPRY
LVPDWTFGIDEENLQRTVDEARAKGARVVVLLSHDGMDVDLKLASRVSGIDAILGGHTHDGVPRPVAVKNRSGQTLVTNA
GSNGKFLGVLDLDVRGGKVASHRYRLLPVFSNLLPADPEMAAHIERVRAPFREKLAAPLAVTEGVLYRRGNFNGTYDQLI
LDALMAEKDAEIAFSPGFRWGASLLPGDTITQEDLLAATAISYPNVGVNDMTGATVRAVLEDVCDNIFNPDPYYQQGGDM
VRVGGLEYACHPGARSGERIQDLRLRGKPLEASKTYRVATWASVSEAARDAGGEPVWDVVGRWLRAQKTITPRTPNLPRL
VGVGRDPGMST

Specific function: Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm. Note=Exported from the cell, except a small proportion that is internally localized [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=504, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786687, Length=471, Percent_Identity=25.9023354564756, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790658, Length=244, Percent_Identity=28.2786885245902, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.6.1.45; =3.1.3.5 [H]

Molecular weight: Translated: 61736; Mature: 61736

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRREFMRMLAAAAAGGMTLRARAAGPEGAGLYDLPRHGNVSLLHFTDVHAQLMPLHYRE
CCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCEECCC
PAVNLGAGPARGRPPHLVGKALLSHFGIRPGTREAHALTFLDFEAAARRYGKVGGFAHLA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCHHHHH
TLVKRLRASRPGALLLDGGDTWQGSATALWTDGQDMIDAAKLLGVDVMTGHWEFTLGADR
HHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCEEEECCEEEEECHHH
VKRAVEKDLAGKISFVAQNVHTADFGDPVFEPWVMREVNGVPVAVIGQAFPYTPIANPRY
HHHHHHHHHHHHEEEHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCCCCCE
LVPDWTFGIDEENLQRTVDEARAKGARVVVLLSHDGMDVDLKLASRVSGIDAILGGHTHD
ECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEHHHHHCCHHHHHCCCCCC
GVPRPVAVKNRSGQTLVTNAGSNGKFLGVLDLDVRGGKVASHRYRLLPVFSNLLPADPEM
CCCCCEEEECCCCCEEEEECCCCCCEEEEEEEECCCCEECCCCEEEEHHHHHCCCCCHHH
AAHIERVRAPFREKLAAPLAVTEGVLYRRGNFNGTYDQLILDALMAEKDAEIAFSPGFRW
HHHHHHHHHHHHHHHCCCHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCC
GASLLPGDTITQEDLLAATAISYPNVGVNDMTGATVRAVLEDVCDNIFNPDPYYQQGGDM
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCE
VRVGGLEYACHPGARSGERIQDLRLRGKPLEASKTYRVATWASVSEAARDAGGEPVWDVV
EEECCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCHHHHH
GRWLRAQKTITPRTPNLPRLVGVGRDPGMST
HHHHHHCCCCCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MDRREFMRMLAAAAAGGMTLRARAAGPEGAGLYDLPRHGNVSLLHFTDVHAQLMPLHYRE
CCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCEECCC
PAVNLGAGPARGRPPHLVGKALLSHFGIRPGTREAHALTFLDFEAAARRYGKVGGFAHLA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCHHHHH
TLVKRLRASRPGALLLDGGDTWQGSATALWTDGQDMIDAAKLLGVDVMTGHWEFTLGADR
HHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCEEEECCEEEEECHHH
VKRAVEKDLAGKISFVAQNVHTADFGDPVFEPWVMREVNGVPVAVIGQAFPYTPIANPRY
HHHHHHHHHHHHEEEHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCCCCCE
LVPDWTFGIDEENLQRTVDEARAKGARVVVLLSHDGMDVDLKLASRVSGIDAILGGHTHD
ECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEHHHHHCCHHHHHCCCCCC
GVPRPVAVKNRSGQTLVTNAGSNGKFLGVLDLDVRGGKVASHRYRLLPVFSNLLPADPEM
CCCCCEEEECCCCCEEEEECCCCCCEEEEEEEECCCCEECCCCEEEEHHHHHCCCCCHHH
AAHIERVRAPFREKLAAPLAVTEGVLYRRGNFNGTYDQLILDALMAEKDAEIAFSPGFRW
HHHHHHHHHHHHHHHCCCHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCC
GASLLPGDTITQEDLLAATAISYPNVGVNDMTGATVRAVLEDVCDNIFNPDPYYQQGGDM
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCE
VRVGGLEYACHPGARSGERIQDLRLRGKPLEASKTYRVATWASVSEAARDAGGEPVWDVV
EEECCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCHHHHH
GRWLRAQKTITPRTPNLPRLVGVGRDPGMST
HHHHHHCCCCCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3012467; 9278503; 9298646; 10331872; 11491293; 11491294 [H]