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The map label for this gene is nuoN [H]

Identifier: 220916474

GI number: 220916474

Start: 1532248

End: 1533798

Strand: Reverse

Name: nuoN [H]

Synonym: A2cp1_1368

Alternate gene names: 220916474

Gene position: 1533798-1532248 (Counterclockwise)

Preceding gene: 220916475

Following gene: 220916472

Centisome position: 30.5

GC content: 69.63

Gene sequence:

>1551_bases
ATGACGGACCTCATCCCACTGGTCGAAGAGAACCTGCGCTCCACCGCGTGGTTCCGCCCCGAGGCGGCCCTCACGGTGGG
CGCGCTGGTCCTCTTCGTGCTGGACATCACCTGGAAGAAGAGCGCGACCCGCGTGGCCTGGCTGAGCGCCGCCTCGCTGG
TGGTGTTCGCGGCCGCGGCGGCGCTGCTGGCGCAGCAGCCCGCCGACGCGCAGACGCTGTTCAACGGCATGCTCGCGAAC
GACGCGTTCGCGATCTTCTTCAAGTGGCTGTTCCTCGGCGCCGGCGCGCTGACCGTGCTCATCACCGCGCAGGGCAAGGA
CTTCGACGCCTCGCGGGTCGGCCAGTTCTACGGCCTGCTCACCGCCGTCGTGCTCGGCCTGTTCATGATGGCGAGCGCGA
CCGACCTGCTCATGATGTACATGTCGATCGAGCTCGTCTCGATCGTGAGCTACGTGCTCGCCGGCTTCCGCCGCGCGGAC
CGCAAGGCCGCCGAGGGCTCGCTGAAGTACGTCATCTACGGCGGCGTGGCCTCGGGCGTGATGCTGTTCGGCATGAGCTA
CCTGTACGGCCTGACCGGCACCACCAGCCTGCTCGAGCTGGGGCCGCGCATCCAGGCCATCCAGGCGTCGGGCCTGCCGC
TCGCGGCCACCCGCATCGCGCTGGTGGTCGGCGCCGTGTTCGTCGCCGCGGGCCTCGGCTACAAGATCGCCGCGGTCCCG
TTCCACATGTGGTGCCCGGACGTGTACGAGGGCGCGCCGACGCCGTTCACCGCGTTCCTGTCGGTCGGCCCGAAGGCGGC
CGGCTTCGCGCTGGCGCTCCGCTTCTTCCTGTCGGCGTTCGCGGGCCCGGCCTCCCCCACCACCGGCCTGGCCGAGGCGC
TGGGCGGCATCCCCTGGCCGGCGGTGATCGGCGTGGTCTCGGCCGTCACCATGACGCTCGGGAACCTGAGCGCGCTCGCG
CAGACCAACCTGAAGCGCCTGCTCGCGTACTCCTCCATCGCGCACGCCGGCTACATGCTCATGGGCCTCGCGACGGTGTC
GGCCATGGGCGTGCAGTCGGTGATGATCTACATGCTCGTGTACCTGGTGATGAACCTGGGCGCGTTCCTGGTCGTCATCT
GGGTGGCGGAGTCCACCGGCTCCGAGTCGATCCTCGACTACCGGGGCCTGTCGAAGCGGGCGCCGGTCGCGGCGGTCGCG
TTCGCCATCTTCCTGTTCTCGCTCACCGGCATCCCGCCGTTCGCCGGGTTCGCGGGCAAGTGGTACCTGTTCTACGCCGT
CTTCGAGCGGATCCCCGGCCCGGGCGGCAACTGGTACGCGCTGCTCGCGGTCATCGCGGCGCTCAACACCGCGGTGGCGC
TCTTCTATTACGTGCGGATCGTCCGCGCGATGTTCATCGACGTGCCGTACGCGGAGCCGAAGCCCATGCCGGCGAAGCCC
GGCTACCAGCTCATGCTGGGCGCGTTCTCGGTGCTGATCCTGATCTTCGGCGTCTGGTGGGCGCCGATGATCGAGTGGAC
GCGCGCGTCGCTGCCGCTGTTCCGGGGCTAG

Upstream 100 bases:

>100_bases
TACCTGGGCTTCTACCCGCAGGCGATCCTGGGCGTCATCAACCCGGCCCTGCACGCCCTCATCCAGAACATCAAGCCGCT
GTAGGCCCGAGGGGCGACGC

Downstream 100 bases:

>100_bases
TAGCGGGAACCCTCCCCCCGCGAGGCCCTCCGGCCCGACCAGCCCCCGCTGGTCGGGCTTTTCATTGCGAGGGCTGCGCC
CTCGCCCCGCCGGCGGAGCC

Product: proton-translocating NADH-quinone oxidoreductase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTDLIPLVEENLRSTAWFRPEAALTVGALVLFVLDITWKKSATRVAWLSAASLVVFAAAAALLAQQPADAQTLFNGMLAN
DAFAIFFKWLFLGAGALTVLITAQGKDFDASRVGQFYGLLTAVVLGLFMMASATDLLMMYMSIELVSIVSYVLAGFRRAD
RKAAEGSLKYVIYGGVASGVMLFGMSYLYGLTGTTSLLELGPRIQAIQASGLPLAATRIALVVGAVFVAAGLGYKIAAVP
FHMWCPDVYEGAPTPFTAFLSVGPKAAGFALALRFFLSAFAGPASPTTGLAEALGGIPWPAVIGVVSAVTMTLGNLSALA
QTNLKRLLAYSSIAHAGYMLMGLATVSAMGVQSVMIYMLVYLVMNLGAFLVVIWVAESTGSESILDYRGLSKRAPVAAVA
FAIFLFSLTGIPPFAGFAGKWYLFYAVFERIPGPGGNWYALLAVIAALNTAVALFYYVRIVRAMFIDVPYAEPKPMPAKP
GYQLMLGAFSVLILIFGVWWAPMIEWTRASLPLFRG

Sequences:

>Translated_516_residues
MTDLIPLVEENLRSTAWFRPEAALTVGALVLFVLDITWKKSATRVAWLSAASLVVFAAAAALLAQQPADAQTLFNGMLAN
DAFAIFFKWLFLGAGALTVLITAQGKDFDASRVGQFYGLLTAVVLGLFMMASATDLLMMYMSIELVSIVSYVLAGFRRAD
RKAAEGSLKYVIYGGVASGVMLFGMSYLYGLTGTTSLLELGPRIQAIQASGLPLAATRIALVVGAVFVAAGLGYKIAAVP
FHMWCPDVYEGAPTPFTAFLSVGPKAAGFALALRFFLSAFAGPASPTTGLAEALGGIPWPAVIGVVSAVTMTLGNLSALA
QTNLKRLLAYSSIAHAGYMLMGLATVSAMGVQSVMIYMLVYLVMNLGAFLVVIWVAESTGSESILDYRGLSKRAPVAAVA
FAIFLFSLTGIPPFAGFAGKWYLFYAVFERIPGPGGNWYALLAVIAALNTAVALFYYVRIVRAMFIDVPYAEPKPMPAKP
GYQLMLGAFSVLILIFGVWWAPMIEWTRASLPLFRG
>Mature_515_residues
TDLIPLVEENLRSTAWFRPEAALTVGALVLFVLDITWKKSATRVAWLSAASLVVFAAAAALLAQQPADAQTLFNGMLAND
AFAIFFKWLFLGAGALTVLITAQGKDFDASRVGQFYGLLTAVVLGLFMMASATDLLMMYMSIELVSIVSYVLAGFRRADR
KAAEGSLKYVIYGGVASGVMLFGMSYLYGLTGTTSLLELGPRIQAIQASGLPLAATRIALVVGAVFVAAGLGYKIAAVPF
HMWCPDVYEGAPTPFTAFLSVGPKAAGFALALRFFLSAFAGPASPTTGLAEALGGIPWPAVIGVVSAVTMTLGNLSALAQ
TNLKRLLAYSSIAHAGYMLMGLATVSAMGVQSVMIYMLVYLVMNLGAFLVVIWVAESTGSESILDYRGLSKRAPVAAVAF
AIFLFSLTGIPPFAGFAGKWYLFYAVFERIPGPGGNWYALLAVIAALNTAVALFYYVRIVRAMFIDVPYAEPKPMPAKPG
YQLMLGAFSVLILIFGVWWAPMIEWTRASLPLFRG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=493, Percent_Identity=30.6288032454361, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1788829, Length=333, Percent_Identity=27.027027027027, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788614, Length=318, Percent_Identity=27.0440251572327, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788827, Length=440, Percent_Identity=23.4090909090909, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788613, Length=321, Percent_Identity=26.1682242990654, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788831, Length=320, Percent_Identity=23.4375, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI2367154, Length=194, Percent_Identity=27.319587628866, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55033; Mature: 54902

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLIPLVEENLRSTAWFRPEAALTVGALVLFVLDITWKKSATRVAWLSAASLVVFAAAA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ALLAQQPADAQTLFNGMLANDAFAIFFKWLFLGAGALTVLITAQGKDFDASRVGQFYGLL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHH
TAVVLGLFMMASATDLLMMYMSIELVSIVSYVLAGFRRADRKAAEGSLKYVIYGGVASGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MLFGMSYLYGLTGTTSLLELGPRIQAIQASGLPLAATRIALVVGAVFVAAGLGYKIAAVP
HHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEECC
FHMWCPDVYEGAPTPFTAFLSVGPKAAGFALALRFFLSAFAGPASPTTGLAEALGGIPWP
HHHCCCHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHH
AVIGVVSAVTMTLGNLSALAQTNLKRLLAYSSIAHAGYMLMGLATVSAMGVQSVMIYMLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLVMNLGAFLVVIWVAESTGSESILDYRGLSKRAPVAAVAFAIFLFSLTGIPPFAGFAGK
HHHHHHHHHHHHHHEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHH
WYLFYAVFERIPGPGGNWYALLAVIAALNTAVALFYYVRIVRAMFIDVPYAEPKPMPAKP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GYQLMLGAFSVLILIFGVWWAPMIEWTRASLPLFRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDLIPLVEENLRSTAWFRPEAALTVGALVLFVLDITWKKSATRVAWLSAASLVVFAAAA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ALLAQQPADAQTLFNGMLANDAFAIFFKWLFLGAGALTVLITAQGKDFDASRVGQFYGLL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHH
TAVVLGLFMMASATDLLMMYMSIELVSIVSYVLAGFRRADRKAAEGSLKYVIYGGVASGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MLFGMSYLYGLTGTTSLLELGPRIQAIQASGLPLAATRIALVVGAVFVAAGLGYKIAAVP
HHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEECC
FHMWCPDVYEGAPTPFTAFLSVGPKAAGFALALRFFLSAFAGPASPTTGLAEALGGIPWP
HHHCCCHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHH
AVIGVVSAVTMTLGNLSALAQTNLKRLLAYSSIAHAGYMLMGLATVSAMGVQSVMIYMLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLVMNLGAFLVVIWVAESTGSESILDYRGLSKRAPVAAVAFAIFLFSLTGIPPFAGFAGK
HHHHHHHHHHHHHHEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHH
WYLFYAVFERIPGPGGNWYALLAVIAALNTAVALFYYVRIVRAMFIDVPYAEPKPMPAKP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GYQLMLGAFSVLILIFGVWWAPMIEWTRASLPLFRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA