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The map label for this gene is gltX [H]

Identifier: 220916458

GI number: 220916458

Start: 1512488

End: 1513912

Strand: Reverse

Name: gltX [H]

Synonym: A2cp1_1352

Alternate gene names: 220916458

Gene position: 1513912-1512488 (Counterclockwise)

Preceding gene: 220916461

Following gene: 220916457

Centisome position: 30.1

GC content: 71.23

Gene sequence:

>1425_bases
ATGGACAAGCCTCGCGTACGCTTCGCCCCCTCCCCCACCGGTTACCTCCACATCGGCGGCGCCCGCACGGCGCTCTTCAA
CTGGCTCTGGGCGCGGCGCAACGGCGGCACCTTCGTGCTGCGCATCGAGGACACCGATCGCGAGCGCTCCACCCAGCTCG
CCGTGGACGCCATCCTCGACGGGCTGCGCTGGCTCGGGCTCGACTGGGACGAGGGCCCTGGCGTCGGCGGGCCGCACCCG
CCCTACTTCCAGACCGAGCGGCTGGACCTCTACAAGGCGCACGCCGAGCGACTCATCCGGGAGGGCAAGGCGTACGCGTG
CTACTGCACGCGCGAGGAGCTGGACGCGCAGCGCAAGCAGGCCGAGGCCGAGAAGCGCCAGTTCCGGTACCCCGGCACCT
GCCGCGACAAGCCCTACGACCCCTCGCGCCCGCACGTGGTCCGCTTCCGGGTGCCGGACGCGGGCGCCACGAGCTGGAAC
GATCTCGTGAAGGGCGTCATCTCGACGCCGCACGACACGCTGCAGGACGAGGTCATCCTGCGCGGCGACGGCGTGCCGCT
CTACAACTTCGGCGCGGTGGTGGACGACATCACCATGGAGATCAACCTGGTCGGCCGCGGCGACGACCACGTGAACAACA
CCGCGCGCCAGATCCTCATGTACGAGGCGCTCGGCTACCCGGTGCCGACGTTCGCGCACTTCCCGATGATCCTGGGCGCG
GACAAGGCCCGGCTCTCCAAGCGCCACGGCGCGACCAGCGTCACCGCGTACCGCGACATGGGCTTCCTGCCCGAGGCGGT
GGTGAACTACCTCGTCCGCCTGGGCTGGTCGCACGGCGACCAGGAGCTCTTCACGCTCGACGAGCTCGTCCGCTACTTCG
ACCTGAAGGACGTGGGCGCGACCGCGGGCGTGTTCAACCCGGAGAAGATGGCCTGGGTGAACCACGAGTGGCTGAAGCGG
CGCTCGCCCGAGGAGCTGGCGAAGCTGGCGCTGCCGCACTTCCGCGCGGCCGGGCTGCCCGCGGAAGACGACGAGAAGCT
GCGGCACGTCTGCGCCGTGGCGCGGGAGCGCGCCAAGACGCTGGGCGAGTACGTGCAGCAGTTCCGCTACTTCTACGCGC
CCATCGCGCTCGATCCGAAGGCGAAGGCCAAGTTCCTGACCGCCGACACGCGGCCGGTGCTCCAGGCCGTCCGCGACGCC
ATCGCCGCGCTCCCCGCTCTCGAGACCCAGGCGGTGGAGCAGGTGTTCCACGGCGAGGCCGAGCGGCGCGGGGTGGGCCT
GGGCAAGGTGGCGCAGCCGGTCCGGGTGGCGCTCACCGGCGGCACCGCCTCCCCCGGCATGTACGACGTGGTGCAGATCC
TCGGGAAGGACGAGACGCTGAAGCGCCTCGACGAGGCCCTCCGGATCGCCGGCGAGCCGGGCTGA

Upstream 100 bases:

>100_bases
GCGGAAGGTCGCGGCCGCTGCCGGAACGGCCAGCGCGGCGAGGAGCAGGGCGAGCGCTCTGTGCACCGGACGATTGTAGC
TATGGTAGCTTGCGCCCGCC

Downstream 100 bases:

>100_bases
AATACGGGCGGGGCCCGTGCGTAGGATCCGGCACTCGCCCGGAGCCCTCCATGCGCGCCAGCCTCGCCGCCACCACCCTC
GCCGCCCTCCTGCTTCCAGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MDKPRVRFAPSPTGYLHIGGARTALFNWLWARRNGGTFVLRIEDTDRERSTQLAVDAILDGLRWLGLDWDEGPGVGGPHP
PYFQTERLDLYKAHAERLIREGKAYACYCTREELDAQRKQAEAEKRQFRYPGTCRDKPYDPSRPHVVRFRVPDAGATSWN
DLVKGVISTPHDTLQDEVILRGDGVPLYNFGAVVDDITMEINLVGRGDDHVNNTARQILMYEALGYPVPTFAHFPMILGA
DKARLSKRHGATSVTAYRDMGFLPEAVVNYLVRLGWSHGDQELFTLDELVRYFDLKDVGATAGVFNPEKMAWVNHEWLKR
RSPEELAKLALPHFRAAGLPAEDDEKLRHVCAVARERAKTLGEYVQQFRYFYAPIALDPKAKAKFLTADTRPVLQAVRDA
IAALPALETQAVEQVFHGEAERRGVGLGKVAQPVRVALTGGTASPGMYDVVQILGKDETLKRLDEALRIAGEPG

Sequences:

>Translated_474_residues
MDKPRVRFAPSPTGYLHIGGARTALFNWLWARRNGGTFVLRIEDTDRERSTQLAVDAILDGLRWLGLDWDEGPGVGGPHP
PYFQTERLDLYKAHAERLIREGKAYACYCTREELDAQRKQAEAEKRQFRYPGTCRDKPYDPSRPHVVRFRVPDAGATSWN
DLVKGVISTPHDTLQDEVILRGDGVPLYNFGAVVDDITMEINLVGRGDDHVNNTARQILMYEALGYPVPTFAHFPMILGA
DKARLSKRHGATSVTAYRDMGFLPEAVVNYLVRLGWSHGDQELFTLDELVRYFDLKDVGATAGVFNPEKMAWVNHEWLKR
RSPEELAKLALPHFRAAGLPAEDDEKLRHVCAVARERAKTLGEYVQQFRYFYAPIALDPKAKAKFLTADTRPVLQAVRDA
IAALPALETQAVEQVFHGEAERRGVGLGKVAQPVRVALTGGTASPGMYDVVQILGKDETLKRLDEALRIAGEPG
>Mature_474_residues
MDKPRVRFAPSPTGYLHIGGARTALFNWLWARRNGGTFVLRIEDTDRERSTQLAVDAILDGLRWLGLDWDEGPGVGGPHP
PYFQTERLDLYKAHAERLIREGKAYACYCTREELDAQRKQAEAEKRQFRYPGTCRDKPYDPSRPHVVRFRVPDAGATSWN
DLVKGVISTPHDTLQDEVILRGDGVPLYNFGAVVDDITMEINLVGRGDDHVNNTARQILMYEALGYPVPTFAHFPMILGA
DKARLSKRHGATSVTAYRDMGFLPEAVVNYLVRLGWSHGDQELFTLDELVRYFDLKDVGATAGVFNPEKMAWVNHEWLKR
RSPEELAKLALPHFRAAGLPAEDDEKLRHVCAVARERAKTLGEYVQQFRYFYAPIALDPKAKAKFLTADTRPVLQAVRDA
IAALPALETQAVEQVFHGEAERRGVGLGKVAQPVRVALTGGTASPGMYDVVQILGKDETLKRLDEALRIAGEPG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=490, Percent_Identity=31.4285714285714, Blast_Score=230, Evalue=3e-60,
Organism=Homo sapiens, GI4826960, Length=299, Percent_Identity=26.7558528428094, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788743, Length=473, Percent_Identity=46.3002114164905, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI226510925, Length=250, Percent_Identity=33.6, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71993394, Length=473, Percent_Identity=31.0782241014799, Blast_Score=235, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6324540, Length=497, Percent_Identity=29.7786720321932, Blast_Score=221, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6324742, Length=116, Percent_Identity=35.3448275862069, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=34.3558282208589, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53039; Mature: 53039

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKPRVRFAPSPTGYLHIGGARTALFNWLWARRNGGTFVLRIEDTDRERSTQLAVDAILD
CCCCCEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHH
GLRWLGLDWDEGPGVGGPHPPYFQTERLDLYKAHAERLIREGKAYACYCTREELDAQRKQ
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
AEAEKRQFRYPGTCRDKPYDPSRPHVVRFRVPDAGATSWNDLVKGVISTPHDTLQDEVIL
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHCCCCEEE
RGDGVPLYNFGAVVDDITMEINLVGRGDDHVNNTARQILMYEALGYPVPTFAHFPMILGA
ECCCCCEEECCHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHCCC
DKARLSKRHGATSVTAYRDMGFLPEAVVNYLVRLGWSHGDQELFTLDELVRYFDLKDVGA
CHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHCCC
TAGVFNPEKMAWVNHEWLKRRSPEELAKLALPHFRAAGLPAEDDEKLRHVCAVARERAKT
CCCCCCCHHHEECCHHHHHCCCHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LGEYVQQFRYFYAPIALDPKAKAKFLTADTRPVLQAVRDAIAALPALETQAVEQVFHGEA
HHHHHHHHHHHHCCEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCH
ERRGVGLGKVAQPVRVALTGGTASPGMYDVVQILGKDETLKRLDEALRIAGEPG
HHCCCCCCHHCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDKPRVRFAPSPTGYLHIGGARTALFNWLWARRNGGTFVLRIEDTDRERSTQLAVDAILD
CCCCCEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHH
GLRWLGLDWDEGPGVGGPHPPYFQTERLDLYKAHAERLIREGKAYACYCTREELDAQRKQ
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
AEAEKRQFRYPGTCRDKPYDPSRPHVVRFRVPDAGATSWNDLVKGVISTPHDTLQDEVIL
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHCCCCEEE
RGDGVPLYNFGAVVDDITMEINLVGRGDDHVNNTARQILMYEALGYPVPTFAHFPMILGA
ECCCCCEEECCHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHCCC
DKARLSKRHGATSVTAYRDMGFLPEAVVNYLVRLGWSHGDQELFTLDELVRYFDLKDVGA
CHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHCCC
TAGVFNPEKMAWVNHEWLKRRSPEELAKLALPHFRAAGLPAEDDEKLRHVCAVARERAKT
CCCCCCCHHHEECCHHHHHCCCHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LGEYVQQFRYFYAPIALDPKAKAKFLTADTRPVLQAVRDAIAALPALETQAVEQVFHGEA
HHHHHHHHHHHHCCEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCH
ERRGVGLGKVAQPVRVALTGGTASPGMYDVVQILGKDETLKRLDEALRIAGEPG
HHCCCCCCHHCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA