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The map label for this gene is fdnH [H]

Identifier: 220915989

GI number: 220915989

Start: 987997

End: 988929

Strand: Reverse

Name: fdnH [H]

Synonym: A2cp1_0875

Alternate gene names: 220915989

Gene position: 988929-987997 (Counterclockwise)

Preceding gene: 220915990

Following gene: 220915988

Centisome position: 19.66

GC content: 73.95

Gene sequence:

>933_bases
GTGCAGGAGATGGGGTTCTTCACCGACACGACGCTCTGCATCGGCTGCAAGGCCTGCGAGGTCGCGTGCAAGCAGTGGAA
CCAGCTCCCCGACGACGGCTTCTCGCTGACCGGGATGAGCTACGACAACACCGGCACGCTGGGCGCGTCCACCTGGCGGC
ACGTCGCGTTCGTCGAGCGGACCGAGCCGCTGCCCGGCCAGGGCAGCCCGCGCGACGGGATCGAGGCGGGCGCGGGCGCG
TTCGCGGAGCTCCGGCCGCTCGGCCAGCCCGCGCCGACGTCGCTGCTCCACGACGTCGCCCCCACCCACCTCGCCGACGT
GCCGGTCGCGCTCGGCCCCTCGCAGCAGGCGCTCGGGAAGTTCTCCTGGCTGATGATGAGCGACGTCTGCAAGCACTGCG
AGCGCGCCGGCTGCCTGGAGGCCTGCCCCACCGGCGCGATCCTCCGCACCGAGTTCGGCTCGGTGTACATCCAGCCGGAC
GTCTGCAACGGCTGCGGCTACTGCGTGTCGGCCTGCCCGTTCGGCGTGGTGGACCGGCGCGAGGACGACGGGCGCGCCTG
GAAGTGCACGCTCTGCTACGACCGGCTCGAGGGCGGCATGGTGCCGGCCTGCGCCAAGGCCTGCCCCACCGCCTCGATCC
AGTTCGGCTCGCTCGCGGACCTGCGCGAGCGGGCCCGGAACCGGGTCGAGCAGCTCCGCGCGCGCGGCGTCGCCGACGCC
CGCCTCTACGGCGAGACCGCGGAGAGCCAGCCCGGGACCGAGGGGCTGCACGCGTTCTTCCTGCTCTGCGACCGGCCCGA
GGTGTACGGGCTCCCGCCGGATCCGGTGGTGCCGACCGCGAAGATCGTGGCGAGCTGGCGCGCCATGGCCACGGGGGTGG
TGTCGCTCGCGGCGCTGGCGCTCGGGGCGGTGCTCTCGGCGAGGAGGGCGTGA

Upstream 100 bases:

>100_bases
GGATCCACGCACCGCGCGACCTCCCCGGCCTGCACGCGAAGCAGCGCAGCCCGCCGGTGGAGCCGCCCGAACCCCGCTGA
TCGCCGCAAGGAGGCGTCCC

Downstream 100 bases:

>100_bases
TGATGGGCACCCGCACGGATCCCGCCCGCGAGTCGGCCCGCCCGCGCGACGGGCGCAACGTGGACCCGCGGCTGGGCGAG
CTGCTGGGAGAGGGCTCGGG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MQEMGFFTDTTLCIGCKACEVACKQWNQLPDDGFSLTGMSYDNTGTLGASTWRHVAFVERTEPLPGQGSPRDGIEAGAGA
FAELRPLGQPAPTSLLHDVAPTHLADVPVALGPSQQALGKFSWLMMSDVCKHCERAGCLEACPTGAILRTEFGSVYIQPD
VCNGCGYCVSACPFGVVDRREDDGRAWKCTLCYDRLEGGMVPACAKACPTASIQFGSLADLRERARNRVEQLRARGVADA
RLYGETAESQPGTEGLHAFFLLCDRPEVYGLPPDPVVPTAKIVASWRAMATGVVSLAALALGAVLSARRA

Sequences:

>Translated_310_residues
MQEMGFFTDTTLCIGCKACEVACKQWNQLPDDGFSLTGMSYDNTGTLGASTWRHVAFVERTEPLPGQGSPRDGIEAGAGA
FAELRPLGQPAPTSLLHDVAPTHLADVPVALGPSQQALGKFSWLMMSDVCKHCERAGCLEACPTGAILRTEFGSVYIQPD
VCNGCGYCVSACPFGVVDRREDDGRAWKCTLCYDRLEGGMVPACAKACPTASIQFGSLADLRERARNRVEQLRARGVADA
RLYGETAESQPGTEGLHAFFLLCDRPEVYGLPPDPVVPTAKIVASWRAMATGVVSLAALALGAVLSARRA
>Mature_310_residues
MQEMGFFTDTTLCIGCKACEVACKQWNQLPDDGFSLTGMSYDNTGTLGASTWRHVAFVERTEPLPGQGSPRDGIEAGAGA
FAELRPLGQPAPTSLLHDVAPTHLADVPVALGPSQQALGKFSWLMMSDVCKHCERAGCLEACPTGAILRTEFGSVYIQPD
VCNGCGYCVSACPFGVVDRREDDGRAWKCTLCYDRLEGGMVPACAKACPTASIQFGSLADLRERARNRVEQLRARGVADA
RLYGETAESQPGTEGLHAFFLLCDRPEVYGLPPDPVVPTAKIVASWRAMATGVVSLAALALGAVLSARRA

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=289, Percent_Identity=33.2179930795848, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790326, Length=311, Percent_Identity=30.8681672025724, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=40.5940594059406, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=37.6237623762376, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=125, Percent_Identity=34.4, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789370, Length=233, Percent_Identity=24.4635193133047, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI226510944, Length=148, Percent_Identity=31.7567567567568, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367245, Length=105, Percent_Identity=41.9047619047619, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787478, Length=106, Percent_Identity=34.9056603773585, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787740, Length=99, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082179, Length=124, Percent_Identity=39.5161290322581, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789067, Length=121, Percent_Identity=39.6694214876033, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788811, Length=106, Percent_Identity=36.7924528301887, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87082180, Length=122, Percent_Identity=33.6065573770492, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 33034; Mature: 33034

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEMGFFTDTTLCIGCKACEVACKQWNQLPDDGFSLTGMSYDNTGTLGASTWRHVAFVER
CCCCCCCCCCHHEECCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHHHEEHCC
TEPLPGQGSPRDGIEAGAGAFAELRPLGQPAPTSLLHDVAPTHLADVPVALGPSQQALGK
CCCCCCCCCCCCCHHHCCCCHHHHCCCCCCCHHHHHHHCCCHHHHCCCEEECCCHHHHHH
FSWLMMSDVCKHCERAGCLEACPTGAILRTEFGSVYIQPDVCNGCGYCVSACPFGVVDRR
HHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCEEECCCCCCCCHHHHHHCCCCCCCCC
EDDGRAWKCTLCYDRLEGGMVPACAKACPTASIQFGSLADLRERARNRVEQLRARGVADA
CCCCCEEEEEEEHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
RLYGETAESQPGTEGLHAFFLLCDRPEVYGLPPDPVVPTAKIVASWRAMATGVVSLAALA
HHHCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGAVLSARRA
HHHHHHHCCC
>Mature Secondary Structure
MQEMGFFTDTTLCIGCKACEVACKQWNQLPDDGFSLTGMSYDNTGTLGASTWRHVAFVER
CCCCCCCCCCHHEECCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHHHEEHCC
TEPLPGQGSPRDGIEAGAGAFAELRPLGQPAPTSLLHDVAPTHLADVPVALGPSQQALGK
CCCCCCCCCCCCCHHHCCCCHHHHCCCCCCCHHHHHHHCCCHHHHCCCEEECCCHHHHHH
FSWLMMSDVCKHCERAGCLEACPTGAILRTEFGSVYIQPDVCNGCGYCVSACPFGVVDRR
HHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCEEECCCCCCCCHHHHHHCCCCCCCCC
EDDGRAWKCTLCYDRLEGGMVPACAKACPTASIQFGSLADLRERARNRVEQLRARGVADA
CCCCCEEEEEEEHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
RLYGETAESQPGTEGLHAFFLLCDRPEVYGLPPDPVVPTAKIVASWRAMATGVVSLAALA
HHHCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGAVLSARRA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]