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The map label for this gene is priA [H]

Identifier: 220915880

GI number: 220915880

Start: 857769

End: 859979

Strand: Reverse

Name: priA [H]

Synonym: A2cp1_0764

Alternate gene names: 220915880

Gene position: 859979-857769 (Counterclockwise)

Preceding gene: 220915882

Following gene: 220915879

Centisome position: 17.1

GC content: 77.61

Gene sequence:

>2211_bases
GTGCTCGTCGAGGTCGCCGTCGCCGCCGCGGTCCGCGGCACCTTCACCTACCGCGTGCCGTCGTCGCTCGCGGCGGAGGT
GGCGCTCGGGCAGCGCGTCGCGGTGCCGTTCGGGAGGAGCCGGCGGGCCACGGGCTACGTGGTCGGCTTCCCCGCCGCGC
CGCCCGAGGGGTTCGAGCTGCGCGACGTGGTCGAGGTCCTCGATCGGTTCCCGCCGTTCACGCCGAAGCTGGTGGAGCTG
CTGCGTTGGGCCGAGGAGTACTACCTCGTGCCGCCCGGCGAGCTCCTGCGGGCCGCGCTGCCGCCCGGCCTGAACGCGCG
CCGCGGGGCGCCGGCCCCGACGCGGCGCGGGGTGGAGTACGCCGCCCCGGCGGCCGACGCCGCCGCGTCGCTCGGCGCGC
TGGGCCGCGCCGCCGCGCAGCGCGCGATCCTCGAGTACCTGCTCGCCCGCGGACGGATCCCGGTGGAGGAGCTGCGCGCC
GCGTTCCCGCGCGGGCGCCCCGCCCTCTCCGCGCTCGCGAAGCGCGGGCTGGTGACGCTCGAGACCGAGACGCCGGTCGC
GACCGCGGGCGTGCTCCCCGCGAGCGCGGCCGCGCCGGCGGCGCTCACCGCGGCCCAGGCGGCGGCGCTGGCGGAGATCG
ACGCGGCCGCCGGCACGTTCCAGGCCTTCCTGCTCCACGGCGTCACCGGCTCGGGCAAGACCGAGGTCTACCTGCAGGCC
ATCGCCCGCGCGCGCGCCGCCGGGCGCGGCGCGCTGGTGCTCGTCCCGGAGATCGCGCTCACCCCGCAGCTCTCCGGCCG
CTTCCGCGCGCGCTTCGGCGACGACGTGGCGCTGCTCCACTCCGGGCTGTCCGACGCCGAGCGCCACGCCGAGTGGCTGC
GCCTCCGCCGCGGCGAGGCGCGGATCTGCGTCGGCGTGCGCAGCGCCATCTTCGCGCCGGTGCAGGACCTGGCGGTGCTG
GTGGTGGACGAGGAGCACGACGGGAGCTTCAAGCAGGAGGACGGCCCGGCCTACCACGCCCGCGACCTCGCGGTGGTGCG
CGCGAAGCGCGAGGACGCGGTGCTGATCCTGGGCTCCGCCACCCCCTCGCTGGAGACGCTGGAGAACGCGCGCCGCGGCC
GGTACCGCAAGCTCGACCTGCCCGCCCGCATCGACGACCGCCCCATGCCCGACGTGGAGCTGGTGGACCTGTCGAAGCTG
CGGCGCGCCGGCGTGACCGGGCTGCCCGGCCTGCTCTCGCCGCGGCTTTGCGCCGCCCTGGAGGACACCCTCGCGGCCGG
CCAGCAGGCCATCGTGTTCCTCAACCGCCGCGGGTACGAGACGCTGGTGGTCTGCGAGGTCTGCGGCGCCGAGGCCCGCT
GCCCGGACTGCTCGGTGTCGCTCACCCACCACGCGCGCCGTGGCGTCCTCATGTGCCACTACTGCGGCCGGACAGAGCCG
ATGAGCGCGCGCTGCGCCGCGTGCGGCGGGGTGCGCTTCGGCGTGGGCGTCGGCACCGAGCAGGTGGAGGAGGCGGTGCG
CTCGCTGCTGCCGCGCGCGCGGGTGGCGCGGCTCGATCGCGACGTGGTCTCGAGCGCCGACGACACCGCCGCGGTGCTGG
CGCGGTTCGCGCGCCGGGACGCCGACGTGCTGGTGGGCACGCAGATGGTGACGAAGGGTCACGACTTCCCCGGCGTCACC
CTGGTCGGCGTGGTGCTCGCCGACACCGCGCTGGCGCTGCCGGACTTCCGCGCCGCCGAGCGCACCTTCCAGCTCCTCAC
CCAGGTGGCCGGGCGCGCCGGGCGCGGGGCCGAGGCCGGGCGGGTGATCGTGCAGACGTTCAACCCCGCCACCCCGGCGG
TCGCGCTCGCCGCCACCCACGACTACGCCGCGTTCGCGGAGGGTGAGCTGGAGCGGCGCCGCGCGTTCGGTTACCCGCCC
TACGGGCGGATGATGGCGGCGCGGGTGGAGGGGACCGAGGACGGCGCGCGCCGCACCGCCGAGGCGCTGGCGCGGGCGGC
CCGGCCGGCGCTGGCCGGCGAGGTGGCCATGCTCGGCCCGGCGCCGGCCGCCATCGAGCGGATCCGCGGGAAGAGCCGCT
GGCACCTGCTGTTCCGCGCCCCCTCGCCGCCGTCGCTGTTCCGGGTCCACGCCGCGCTGGCGCGGGTGGCGCAGCGGCCG
CCCGGCGGCGCCGCGATCCGCTTCGACATGGACCCGTACTCGATGTTGTAG

Upstream 100 bases:

>100_bases
CGGCGATGCGCAGATCGATGCGGGCCATGTGTTCCTCCGTGAGCGGGGGAGTGTAGCCGATGCACGGCGGAGAGGGGGGA
GTGGCTGCTAGACTCCGCGC

Downstream 100 bases:

>100_bases
GTCCGGCACGCACGCCCGCCGGCGGCGCGGCGGCGGCTTGGTCGCCCGGCGAGCCCGATCCATAATCCCCGCGCTTGCCC
CGGATCCTCCTGGTCGACGA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MLVEVAVAAAVRGTFTYRVPSSLAAEVALGQRVAVPFGRSRRATGYVVGFPAAPPEGFELRDVVEVLDRFPPFTPKLVEL
LRWAEEYYLVPPGELLRAALPPGLNARRGAPAPTRRGVEYAAPAADAAASLGALGRAAAQRAILEYLLARGRIPVEELRA
AFPRGRPALSALAKRGLVTLETETPVATAGVLPASAAAPAALTAAQAAALAEIDAAAGTFQAFLLHGVTGSGKTEVYLQA
IARARAAGRGALVLVPEIALTPQLSGRFRARFGDDVALLHSGLSDAERHAEWLRLRRGEARICVGVRSAIFAPVQDLAVL
VVDEEHDGSFKQEDGPAYHARDLAVVRAKREDAVLILGSATPSLETLENARRGRYRKLDLPARIDDRPMPDVELVDLSKL
RRAGVTGLPGLLSPRLCAALEDTLAAGQQAIVFLNRRGYETLVVCEVCGAEARCPDCSVSLTHHARRGVLMCHYCGRTEP
MSARCAACGGVRFGVGVGTEQVEEAVRSLLPRARVARLDRDVVSSADDTAAVLARFARRDADVLVGTQMVTKGHDFPGVT
LVGVVLADTALALPDFRAAERTFQLLTQVAGRAGRGAEAGRVIVQTFNPATPAVALAATHDYAAFAEGELERRRAFGYPP
YGRMMAARVEGTEDGARRTAEALARAARPALAGEVAMLGPAPAAIERIRGKSRWHLLFRAPSPPSLFRVHAALARVAQRP
PGGAAIRFDMDPYSML

Sequences:

>Translated_736_residues
MLVEVAVAAAVRGTFTYRVPSSLAAEVALGQRVAVPFGRSRRATGYVVGFPAAPPEGFELRDVVEVLDRFPPFTPKLVEL
LRWAEEYYLVPPGELLRAALPPGLNARRGAPAPTRRGVEYAAPAADAAASLGALGRAAAQRAILEYLLARGRIPVEELRA
AFPRGRPALSALAKRGLVTLETETPVATAGVLPASAAAPAALTAAQAAALAEIDAAAGTFQAFLLHGVTGSGKTEVYLQA
IARARAAGRGALVLVPEIALTPQLSGRFRARFGDDVALLHSGLSDAERHAEWLRLRRGEARICVGVRSAIFAPVQDLAVL
VVDEEHDGSFKQEDGPAYHARDLAVVRAKREDAVLILGSATPSLETLENARRGRYRKLDLPARIDDRPMPDVELVDLSKL
RRAGVTGLPGLLSPRLCAALEDTLAAGQQAIVFLNRRGYETLVVCEVCGAEARCPDCSVSLTHHARRGVLMCHYCGRTEP
MSARCAACGGVRFGVGVGTEQVEEAVRSLLPRARVARLDRDVVSSADDTAAVLARFARRDADVLVGTQMVTKGHDFPGVT
LVGVVLADTALALPDFRAAERTFQLLTQVAGRAGRGAEAGRVIVQTFNPATPAVALAATHDYAAFAEGELERRRAFGYPP
YGRMMAARVEGTEDGARRTAEALARAARPALAGEVAMLGPAPAAIERIRGKSRWHLLFRAPSPPSLFRVHAALARVAQRP
PGGAAIRFDMDPYSML
>Mature_736_residues
MLVEVAVAAAVRGTFTYRVPSSLAAEVALGQRVAVPFGRSRRATGYVVGFPAAPPEGFELRDVVEVLDRFPPFTPKLVEL
LRWAEEYYLVPPGELLRAALPPGLNARRGAPAPTRRGVEYAAPAADAAASLGALGRAAAQRAILEYLLARGRIPVEELRA
AFPRGRPALSALAKRGLVTLETETPVATAGVLPASAAAPAALTAAQAAALAEIDAAAGTFQAFLLHGVTGSGKTEVYLQA
IARARAAGRGALVLVPEIALTPQLSGRFRARFGDDVALLHSGLSDAERHAEWLRLRRGEARICVGVRSAIFAPVQDLAVL
VVDEEHDGSFKQEDGPAYHARDLAVVRAKREDAVLILGSATPSLETLENARRGRYRKLDLPARIDDRPMPDVELVDLSKL
RRAGVTGLPGLLSPRLCAALEDTLAAGQQAIVFLNRRGYETLVVCEVCGAEARCPDCSVSLTHHARRGVLMCHYCGRTEP
MSARCAACGGVRFGVGVGTEQVEEAVRSLLPRARVARLDRDVVSSADDTAAVLARFARRDADVLVGTQMVTKGHDFPGVT
LVGVVLADTALALPDFRAAERTFQLLTQVAGRAGRGAEAGRVIVQTFNPATPAVALAATHDYAAFAEGELERRRAFGYPP
YGRMMAARVEGTEDGARRTAEALARAARPALAGEVAMLGPAPAAIERIRGKSRWHLLFRAPSPPSLFRVHAALARVAQRP
PGGAAIRFDMDPYSML

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=741, Percent_Identity=39.9460188933873, Blast_Score=464, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 78267; Mature: 78267

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVEVAVAAAVRGTFTYRVPSSLAAEVALGQRVAVPFGRSRRATGYVVGFPAAPPEGFEL
CEEEHHHHHHHHCEEEEECCHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCCCCCCH
RDVVEVLDRFPPFTPKLVELLRWAEEYYLVPPGELLRAALPPGLNARRGAPAPTRRGVEY
HHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AAPAADAAASLGALGRAAAQRAILEYLLARGRIPVEELRAAFPRGRPALSALAKRGLVTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHCCCEEE
ETETPVATAGVLPASAAAPAALTAAQAAALAEIDAAAGTFQAFLLHGVTGSGKTEVYLQA
ECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IARARAAGRGALVLVPEIALTPQLSGRFRARFGDDVALLHSGLSDAERHAEWLRLRRGEA
HHHHHHCCCCEEEEECCEEECCCCCCCEECCCCCCHHHHHCCCCHHHHHHHHHHHHCCCC
RICVGVRSAIFAPVQDLAVLVVDEEHDGSFKQEDGPAYHARDLAVVRAKREDAVLILGSA
EEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHEEEECCCCEEEEEECC
TPSLETLENARRGRYRKLDLPARIDDRPMPDVELVDLSKLRRAGVTGLPGLLSPRLCAAL
CCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHH
EDTLAAGQQAIVFLNRRGYETLVVCEVCGAEARCPDCSVSLTHHARRGVLMCHYCGRTEP
HHHHHCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCEEHHHHCCCCEEEEEECCCCCC
MSARCAACGGVRFGVGVGTEQVEEAVRSLLPRARVARLDRDVVSSADDTAAVLARFARRD
CCCHHHHCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
ADVLVGTQMVTKGHDFPGVTLVGVVLADTALALPDFRAAERTFQLLTQVAGRAGRGAEAG
CCEEECHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
RVIVQTFNPATPAVALAATHDYAAFAEGELERRRAFGYPPYGRMMAARVEGTEDGARRTA
EEEEEECCCCCCEEEEEECCCHHHHHCCHHHHHHHCCCCCHHHEEEEEECCCCHHHHHHH
EALARAARPALAGEVAMLGPAPAAIERIRGKSRWHLLFRAPSPPSLFRVHAALARVAQRP
HHHHHHHCCHHHCCEEECCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
PGGAAIRFDMDPYSML
CCCEEEEECCCHHCCC
>Mature Secondary Structure
MLVEVAVAAAVRGTFTYRVPSSLAAEVALGQRVAVPFGRSRRATGYVVGFPAAPPEGFEL
CEEEHHHHHHHHCEEEEECCHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCCCCCCH
RDVVEVLDRFPPFTPKLVELLRWAEEYYLVPPGELLRAALPPGLNARRGAPAPTRRGVEY
HHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AAPAADAAASLGALGRAAAQRAILEYLLARGRIPVEELRAAFPRGRPALSALAKRGLVTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHCCCEEE
ETETPVATAGVLPASAAAPAALTAAQAAALAEIDAAAGTFQAFLLHGVTGSGKTEVYLQA
ECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IARARAAGRGALVLVPEIALTPQLSGRFRARFGDDVALLHSGLSDAERHAEWLRLRRGEA
HHHHHHCCCCEEEEECCEEECCCCCCCEECCCCCCHHHHHCCCCHHHHHHHHHHHHCCCC
RICVGVRSAIFAPVQDLAVLVVDEEHDGSFKQEDGPAYHARDLAVVRAKREDAVLILGSA
EEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHEEEECCCCEEEEEECC
TPSLETLENARRGRYRKLDLPARIDDRPMPDVELVDLSKLRRAGVTGLPGLLSPRLCAAL
CCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHH
EDTLAAGQQAIVFLNRRGYETLVVCEVCGAEARCPDCSVSLTHHARRGVLMCHYCGRTEP
HHHHHCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCEEHHHHCCCCEEEEEECCCCCC
MSARCAACGGVRFGVGVGTEQVEEAVRSLLPRARVARLDRDVVSSADDTAAVLARFARRD
CCCHHHHCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
ADVLVGTQMVTKGHDFPGVTLVGVVLADTALALPDFRAAERTFQLLTQVAGRAGRGAEAG
CCEEECHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
RVIVQTFNPATPAVALAATHDYAAFAEGELERRRAFGYPPYGRMMAARVEGTEDGARRTA
EEEEEECCCCCCEEEEEECCCHHHHHCCHHHHHHHCCCCCHHHEEEEEECCCCHHHHHHH
EALARAARPALAGEVAMLGPAPAAIERIRGKSRWHLLFRAPSPPSLFRVHAALARVAQRP
HHHHHHHCCHHHCCEEECCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
PGGAAIRFDMDPYSML
CCCEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]