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The map label for this gene is argJ [H]

Identifier: 220915733

GI number: 220915733

Start: 689849

End: 691948

Strand: Reverse

Name: argJ [H]

Synonym: A2cp1_0614

Alternate gene names: 220915733

Gene position: 691948-689849 (Counterclockwise)

Preceding gene: 220915734

Following gene: 220915732

Centisome position: 13.76

GC content: 73.19

Gene sequence:

>2100_bases
GTGAGGATCCCGAAGGGCTTCCGGTTCGGCGGGGTGGCGTGCGGGCTGAAGCCGCAGCGCCGCGACCTCGCGCTCGTCGT
CTCCGACCACCCGGCCGCCGCGGCCGGCCTGTTCACCATCAACCGCGCCGCCGCCGCGCCGGTCATCGACGCGCGCACGC
GCGTGCCGGCCGAGGGCATCCGCGCCATCGTGGTGAACTCCGGGAACGCGAACGCGCTCACCGGCCCGGCCGGCCTCGAC
GACGTCACCGTGATCCGCGCCGCGGTGGCCGACGCGCTCGGGCTCCAGAAGCGCGCGGTGCTCACCGCGTCCACCGGCGT
CATCGGCGCGCGGCTGCCCGCCATGAAGGTGGTCACCGCGCTGCCGGCGCTGGTGGACCAGCTCGGCGACCACCCGGATC
TCGCCGCCGAGGCGATCATGACCACCGACACGCGGCCGAAGATGGCCGCGCGCGAGGTGACGCTGGGCGGGAAGACCGCC
ATCGTGACCGCGATCTGCAAGGGCTCGGGCATGCTCGCGCCGCAGCTCGCGACCACGATCTGCCTGCTCGTCACCGACGC
GGCGGTGACGCCGCGCGCGCTGCAGGACGCGCTGGCACGGGCGGTGGAGCGGACGCTGCACATGGTGACGGTGGACGGCG
AGATGTCCACCAACGACACGGTCTACGCGCTCGCGAACGGGCTGGCCGGCAACCCGCGCATCTCCGACCCGGGGCCGGAC
CTGGATCGCCTGGAGACCGCGCTCACCGACCTGCTGGAGGAGATGGCCCGCGCCATGGCGGCCGACGGCGAGGGCGCCAC
CAAGCTGGTGGAGGTGGTGGTGTCCGGCGCCCCGTCGGACGAGGCGGCACGCGACTGCGCCCGCGCCGTCGCCTCCTCGC
CGCTGGTGAAGGCGGCGCTGTTCGGGGCCGATCCGAACTGGGGCCGCGTGCTCGCCACGGTGGGCGCGCGCGCCGGCGCC
ATGAACTACCCCATCGACCCGTACCGGGCGCGGGTGGTGCTGCAGGGCGTGACGGTGTTCGAGGGCGGCGCGCCGGTCGA
GCTGGATCGCGAGGGGCTCCGGACGCGCATGCGCGACAGCCGCATCGACGTGCTGGTCGAGCTCGCCGACGGGACCGCGC
GCGCGGTGGCCTGGGGCTGCGATCTCTCCTACGACTACGTCAAGATCAACGCCGACTACACCTCGCTCATCGTGCAGCGG
CCCGACGGCGGCGTCGGCAAGGACGACCGCGTCTCCAACTACAGCCCGGCGTTCAAGCGGACCCTGCTCGCCGAGGCGCT
GAAGTACATCGCCGCGTTCTCCGGGCAGGTGGCGGTCATCAAGTACGGCGGCGCCGCCATGGTGAAGGACAGCCTGAAGG
CCGCGTTCGCCGAGGACGTGACGCTGCTGAAGAAGGTCGGGCTGAAGCCGGTGGTGGTGCACGGCGGCGCGCCGGAGATC
ACCCGCACGCTCGAGAAGCTGGGCGAGCGCAGCGAGTTCGTGGACGGCATGCGCATCACCGACGCGCAGAGCCTGCCGGT
GGTGGAGATGGTGCTCACCGGCAAGGTGAACCAGGAGCTGGTGGCGCTGCTCAACGCGCGCAGCGCCGGCGCGGTGGGCC
TGTCCGGCAAGGACGGGCAGCTGCTCCGGGCGCGCAAGGCGGTGCACGAGTCCGGCCGCGACCTCGGCTACGTGGGCGAG
GTGGTGCAGGTGAACACCCAGTTCCTGCGCATGTTCCTGGACGGCGGCTACGTCCCGGTGATCTCGCCCATCGGCCTCAC
CGACGACGGCACCGGCCTGTCGATCAACGCCGACGACGTCGCCGCGGCCATCGCGGTGGCGCTCGGGGCGCCGAAGCTCA
TCTACCTGACCGACGTCGCCGGGGTGCTGGAGTCGGCGCCCGACGGGCAGCTGGTCCGCCAGCTCACGCTCGCCGACCTC
GACCGCCGCATCCAGGCGGGCGCCATCACCGGCGGGATGAAGTGGAAGGCCTGGGCCATCCAGACCGCGCTCCGCGGCGG
GGTGGGCCGGGTGCACGTCCTCGACGGCCGGCAGCCGCACACCGTCATCGCCGAGCTCTTCACCGACCGCGGGGTGGGCT
CGCTCCTCACCGCCGAATGA

Upstream 100 bases:

>100_bases
ACAACCTGGTGAAGGGCGCCGCGGGGCAGGCCGTGCAGTCCATGAACGCCGCGCTGGGCTGGCCGGAGACGGCCGGCCTC
GACCTGCTGGGAGGCTAGCG

Downstream 100 bases:

>100_bases
CCTCCGCCGACCGCCGCCGCGACGCCGTCGCCCGCATCATCCGCGCCCGCCGCATCGGGACGCAGGAGGAGCTGCTCGCC
GCGCTGGAGCGGGCCGGCTT

Product: arginine biosynthesis bifunctional protein ArgJ

Products: NA

Alternate protein names: Glutamate N-acetyltransferase; Ornithine acetyltransferase; OATase; Ornithine transacetylase; Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS [H]

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MRIPKGFRFGGVACGLKPQRRDLALVVSDHPAAAAGLFTINRAAAAPVIDARTRVPAEGIRAIVVNSGNANALTGPAGLD
DVTVIRAAVADALGLQKRAVLTASTGVIGARLPAMKVVTALPALVDQLGDHPDLAAEAIMTTDTRPKMAAREVTLGGKTA
IVTAICKGSGMLAPQLATTICLLVTDAAVTPRALQDALARAVERTLHMVTVDGEMSTNDTVYALANGLAGNPRISDPGPD
LDRLETALTDLLEEMARAMAADGEGATKLVEVVVSGAPSDEAARDCARAVASSPLVKAALFGADPNWGRVLATVGARAGA
MNYPIDPYRARVVLQGVTVFEGGAPVELDREGLRTRMRDSRIDVLVELADGTARAVAWGCDLSYDYVKINADYTSLIVQR
PDGGVGKDDRVSNYSPAFKRTLLAEALKYIAAFSGQVAVIKYGGAAMVKDSLKAAFAEDVTLLKKVGLKPVVVHGGAPEI
TRTLEKLGERSEFVDGMRITDAQSLPVVEMVLTGKVNQELVALLNARSAGAVGLSGKDGQLLRARKAVHESGRDLGYVGE
VVQVNTQFLRMFLDGGYVPVISPIGLTDDGTGLSINADDVAAAIAVALGAPKLIYLTDVAGVLESAPDGQLVRQLTLADL
DRRIQAGAITGGMKWKAWAIQTALRGGVGRVHVLDGRQPHTVIAELFTDRGVGSLLTAE

Sequences:

>Translated_699_residues
MRIPKGFRFGGVACGLKPQRRDLALVVSDHPAAAAGLFTINRAAAAPVIDARTRVPAEGIRAIVVNSGNANALTGPAGLD
DVTVIRAAVADALGLQKRAVLTASTGVIGARLPAMKVVTALPALVDQLGDHPDLAAEAIMTTDTRPKMAAREVTLGGKTA
IVTAICKGSGMLAPQLATTICLLVTDAAVTPRALQDALARAVERTLHMVTVDGEMSTNDTVYALANGLAGNPRISDPGPD
LDRLETALTDLLEEMARAMAADGEGATKLVEVVVSGAPSDEAARDCARAVASSPLVKAALFGADPNWGRVLATVGARAGA
MNYPIDPYRARVVLQGVTVFEGGAPVELDREGLRTRMRDSRIDVLVELADGTARAVAWGCDLSYDYVKINADYTSLIVQR
PDGGVGKDDRVSNYSPAFKRTLLAEALKYIAAFSGQVAVIKYGGAAMVKDSLKAAFAEDVTLLKKVGLKPVVVHGGAPEI
TRTLEKLGERSEFVDGMRITDAQSLPVVEMVLTGKVNQELVALLNARSAGAVGLSGKDGQLLRARKAVHESGRDLGYVGE
VVQVNTQFLRMFLDGGYVPVISPIGLTDDGTGLSINADDVAAAIAVALGAPKLIYLTDVAGVLESAPDGQLVRQLTLADL
DRRIQAGAITGGMKWKAWAIQTALRGGVGRVHVLDGRQPHTVIAELFTDRGVGSLLTAE
>Mature_699_residues
MRIPKGFRFGGVACGLKPQRRDLALVVSDHPAAAAGLFTINRAAAAPVIDARTRVPAEGIRAIVVNSGNANALTGPAGLD
DVTVIRAAVADALGLQKRAVLTASTGVIGARLPAMKVVTALPALVDQLGDHPDLAAEAIMTTDTRPKMAAREVTLGGKTA
IVTAICKGSGMLAPQLATTICLLVTDAAVTPRALQDALARAVERTLHMVTVDGEMSTNDTVYALANGLAGNPRISDPGPD
LDRLETALTDLLEEMARAMAADGEGATKLVEVVVSGAPSDEAARDCARAVASSPLVKAALFGADPNWGRVLATVGARAGA
MNYPIDPYRARVVLQGVTVFEGGAPVELDREGLRTRMRDSRIDVLVELADGTARAVAWGCDLSYDYVKINADYTSLIVQR
PDGGVGKDDRVSNYSPAFKRTLLAEALKYIAAFSGQVAVIKYGGAAMVKDSLKAAFAEDVTLLKKVGLKPVVVHGGAPEI
TRTLEKLGERSEFVDGMRITDAQSLPVVEMVLTGKVNQELVALLNARSAGAVGLSGKDGQLLRARKAVHESGRDLGYVGE
VVQVNTQFLRMFLDGGYVPVISPIGLTDDGTGLSINADDVAAAIAVALGAPKLIYLTDVAGVLESAPDGQLVRQLTLADL
DRRIQAGAITGGMKWKAWAIQTALRGGVGRVHVLDGRQPHTVIAELFTDRGVGSLLTAE

Specific function: Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis:the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate [H]

COG id: COG1364

COG function: function code E; N-acetylglutamate synthase (N-acetylornithine aminotransferase)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArgJ family [H]

Homologues:

Organism=Escherichia coli, GI1789181, Length=293, Percent_Identity=23.8907849829352, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693208, Length=228, Percent_Identity=31.5789473684211, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323707, Length=415, Percent_Identity=35.1807228915663, Blast_Score=236, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6320913, Length=289, Percent_Identity=27.681660899654, Blast_Score=87, Evalue=7e-18,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002813
- InterPro:   IPR016117 [H]

Pfam domain/function: PF01960 ArgJ [H]

EC number: =2.3.1.35; =2.3.1.1 [H]

Molecular weight: Translated: 72992; Mature: 72992

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPKGFRFGGVACGLKPQRRDLALVVSDHPAAAAGLFTINRAAAAPVIDARTRVPAEGI
CCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCHHHHEEEHHHHCCCEECCCCCCCCCCC
RAIVVNSGNANALTGPAGLDDVTVIRAAVADALGLQKRAVLTASTGVIGARLPAMKVVTA
EEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCHHEEEEECCCCHHHCCHHHHHHHH
LPALVDQLGDHPDLAAEAIMTTDTRPKMAAREVTLGGKTAIVTAICKGSGMLAPQLATTI
HHHHHHHHCCCCCHHHHHHEECCCCCCHHHHEEECCCCEEEEEEEECCCCCCCHHHHHHH
CLLVTDAAVTPRALQDALARAVERTLHMVTVDGEMSTNDTVYALANGLAGNPRISDPGPD
HHHHCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCC
LDRLETALTDLLEEMARAMAADGEGATKLVEVVVSGAPSDEAARDCARAVASSPLVKAAL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHE
FGADPNWGRVLATVGARAGAMNYPIDPYRARVVLQGVTVFEGGAPVELDREGLRTRMRDS
ECCCCCCCHHHHHHCCCCCCCCCCCCHHHEEEEEEEEEEECCCCCCEECHHHHHHHHCCC
RIDVLVELADGTARAVAWGCDLSYDYVKINADYTSLIVQRPDGGVGKDDRVSNYSPAFKR
CEEEEEEECCCCEEEEEEECCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCHHHHH
TLLAEALKYIAAFSGQVAVIKYGGAAMVKDSLKAAFAEDVTLLKKVGLKPVVVHGGAPEI
HHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
TRTLEKLGERSEFVDGMRITDAQSLPVVEMVLTGKVNQELVALLNARSAGAVGLSGKDGQ
HHHHHHHCCHHHHHCCCEECCCCCCCCEEHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCH
LLRARKAVHESGRDLGYVGEVVQVNTQFLRMFLDGGYVPVISPIGLTDDGTGLSINADDV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEECHHHH
AAAIAVALGAPKLIYLTDVAGVLESAPDGQLVRQLTLADLDRRIQAGAITGGMKWKAWAI
HHHHHHHHCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEECCCCCHHHHH
QTALRGGVGRVHVLDGRQPHTVIAELFTDRGVGSLLTAE
HHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRIPKGFRFGGVACGLKPQRRDLALVVSDHPAAAAGLFTINRAAAAPVIDARTRVPAEGI
CCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCHHHHEEEHHHHCCCEECCCCCCCCCCC
RAIVVNSGNANALTGPAGLDDVTVIRAAVADALGLQKRAVLTASTGVIGARLPAMKVVTA
EEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCHHEEEEECCCCHHHCCHHHHHHHH
LPALVDQLGDHPDLAAEAIMTTDTRPKMAAREVTLGGKTAIVTAICKGSGMLAPQLATTI
HHHHHHHHCCCCCHHHHHHEECCCCCCHHHHEEECCCCEEEEEEEECCCCCCCHHHHHHH
CLLVTDAAVTPRALQDALARAVERTLHMVTVDGEMSTNDTVYALANGLAGNPRISDPGPD
HHHHCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCC
LDRLETALTDLLEEMARAMAADGEGATKLVEVVVSGAPSDEAARDCARAVASSPLVKAAL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHE
FGADPNWGRVLATVGARAGAMNYPIDPYRARVVLQGVTVFEGGAPVELDREGLRTRMRDS
ECCCCCCCHHHHHHCCCCCCCCCCCCHHHEEEEEEEEEEECCCCCCEECHHHHHHHHCCC
RIDVLVELADGTARAVAWGCDLSYDYVKINADYTSLIVQRPDGGVGKDDRVSNYSPAFKR
CEEEEEEECCCCEEEEEEECCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCHHHHH
TLLAEALKYIAAFSGQVAVIKYGGAAMVKDSLKAAFAEDVTLLKKVGLKPVVVHGGAPEI
HHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
TRTLEKLGERSEFVDGMRITDAQSLPVVEMVLTGKVNQELVALLNARSAGAVGLSGKDGQ
HHHHHHHCCHHHHHCCCEECCCCCCCCEEHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCH
LLRARKAVHESGRDLGYVGEVVQVNTQFLRMFLDGGYVPVISPIGLTDDGTGLSINADDV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEECHHHH
AAAIAVALGAPKLIYLTDVAGVLESAPDGQLVRQLTLADLDRRIQAGAITGGMKWKAWAI
HHHHHHHHCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEECCCCCHHHHH
QTALRGGVGRVHVLDGRQPHTVIAELFTDRGVGSLLTAE
HHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]