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The map label for this gene is yfjR [H]

Identifier: 220915729

GI number: 220915729

Start: 685767

End: 686648

Strand: Reverse

Name: yfjR [H]

Synonym: A2cp1_0610

Alternate gene names: 220915729

Gene position: 686648-685767 (Counterclockwise)

Preceding gene: 220915731

Following gene: 220915725

Centisome position: 13.65

GC content: 76.3

Gene sequence:

>882_bases
ATGCGCTTGCGGATCGGCTTCATCGGCCTCGGGACGATGGGCGAGCCCATCGCCAACAACCTGCGCCGCGCTGGCCACGA
GCTCGCGGTCTGGAACCGCACGCCGGCCCGGGCGGATCACCTCGTGTCCAGGGGCGCGAAGCGCGCCGCCACGCCGCGCG
AGTGCGCGAGCGGCAGGGACGCGGTGTTCACCTGCGTGTCCGACGAGCGCGCGCTCGACGCGGTCCTCGACGGGCCCGAC
GGCGTGCTCGCCGGGCTGCGCGAGGGCGACGTGCTGGTGGACCTCACCACCGCGGGCGTCACCTCCGCCCGCGCGGTCGC
CGCCCGCGCGGACGCGCGGGGCGCGCGCTTCGTCGCCTGCCCGGTGCTCGGCTCGAAGACGGCCGCGGAGCAGGCGCAGG
TCGTGCTGGTGGCGGGCGGCCCCGGTCCGGCCCGCGAGCGGCTCCGCCCGGCGCTCCACGCGATCTCGGCCCGGCTGTTC
GAGCTTGACGACCCGGCGCAGGCCGCGCTGATGAAGCTGTGCGTGAACGCGGTCGGCGGCGCGATGATGACCGCGTTCGG
CGAGGCGGTGGCGCTCGCCTCCGCGGGCGGCGTCGAGCCGTCGCGCCTGGTGGAGGTGCTGCAGGCGTCCGCCTTCCACT
CGCCGCTCTACCTCATGAAGGGCGAGCTGGTGCAGAAGAAGGACTGGGCGCCGCGGTTCCGCATCGCGCTCGCGGAGAAG
GACCAGCGCCTCGCGCAGGAGGCCGCGGCCGAGCTGGGCGCGCAGGTCCCGGTCAGCGAGGCGGTCCGCGAGCTGATGGC
CGCCGCGACCGAGGCCGGCCGCGGGGACCAGGACGTCGCGGCGCTCGCCGACCTCTACCTGGAGTGGACGCGGAGGCGGT
GA

Upstream 100 bases:

>100_bases
CCAGAGGGCTTTTCGCCGGCCTCCCGCACGGCCGCGGGGCGCGCCCGCGCGGCGCTCCGCCGCGCTGGCCGGGGCCGTTC
GATTGATCTAGAGATCCCGG

Downstream 100 bases:

>100_bases
CGCGGGTCGCGCCTACGGCGCACCTTCCGCCAGCGCGGGTCGCGTCGGTCCGGCGGGCTCGAGCGCCTCGGCCACGGCGC
GGACCAGATCGTCGAGGTGG

Product: 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRLRIGFIGLGTMGEPIANNLRRAGHELAVWNRTPARADHLVSRGAKRAATPRECASGRDAVFTCVSDERALDAVLDGPD
GVLAGLREGDVLVDLTTAGVTSARAVAARADARGARFVACPVLGSKTAAEQAQVVLVAGGPGPARERLRPALHAISARLF
ELDDPAQAALMKLCVNAVGGAMMTAFGEAVALASAGGVEPSRLVEVLQASAFHSPLYLMKGELVQKKDWAPRFRIALAEK
DQRLAQEAAAELGAQVPVSEAVRELMAAATEAGRGDQDVAALADLYLEWTRRR

Sequences:

>Translated_293_residues
MRLRIGFIGLGTMGEPIANNLRRAGHELAVWNRTPARADHLVSRGAKRAATPRECASGRDAVFTCVSDERALDAVLDGPD
GVLAGLREGDVLVDLTTAGVTSARAVAARADARGARFVACPVLGSKTAAEQAQVVLVAGGPGPARERLRPALHAISARLF
ELDDPAQAALMKLCVNAVGGAMMTAFGEAVALASAGGVEPSRLVEVLQASAFHSPLYLMKGELVQKKDWAPRFRIALAEK
DQRLAQEAAAELGAQVPVSEAVRELMAAATEAGRGDQDVAALADLYLEWTRRR
>Mature_293_residues
MRLRIGFIGLGTMGEPIANNLRRAGHELAVWNRTPARADHLVSRGAKRAATPRECASGRDAVFTCVSDERALDAVLDGPD
GVLAGLREGDVLVDLTTAGVTSARAVAARADARGARFVACPVLGSKTAAEQAQVVLVAGGPGPARERLRPALHAISARLF
ELDDPAQAALMKLCVNAVGGAMMTAFGEAVALASAGGVEPSRLVEVLQASAFHSPLYLMKGELVQKKDWAPRFRIALAEK
DQRLAQEAAAELGAQVPVSEAVRELMAAATEAGRGDQDVAALADLYLEWTRRR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=31.5789473684211, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI23308751, Length=285, Percent_Identity=21.7543859649123, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI145693186, Length=294, Percent_Identity=27.891156462585, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1786719, Length=280, Percent_Identity=28.9285714285714, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790315, Length=279, Percent_Identity=31.1827956989247, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1789092, Length=292, Percent_Identity=25, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557316, Length=268, Percent_Identity=24.6268656716418, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=25.3571428571429, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24655230, Length=289, Percent_Identity=26.643598615917, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922568, Length=289, Percent_Identity=26.643598615917, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30847; Mature: 30847

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRIGFIGLGTMGEPIANNLRRAGHELAVWNRTPARADHLVSRGAKRAATPRECASGRD
CEEEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHCCHHHCCCCHHHHCCCC
AVFTCVSDERALDAVLDGPDGVLAGLREGDVLVDLTTAGVTSARAVAARADARGARFVAC
EEEEEECCCHHHHHHHCCCCHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEE
PVLGSKTAAEQAQVVLVAGGPGPARERLRPALHAISARLFELDDPAQAALMKLCVNAVGG
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AMMTAFGEAVALASAGGVEPSRLVEVLQASAFHSPLYLMKGELVQKKDWAPRFRIALAEK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCCEEEEEHHH
DQRLAQEAAAELGAQVPVSEAVRELMAAATEAGRGDQDVAALADLYLEWTRRR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLRIGFIGLGTMGEPIANNLRRAGHELAVWNRTPARADHLVSRGAKRAATPRECASGRD
CEEEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHCCHHHCCCCHHHHCCCC
AVFTCVSDERALDAVLDGPDGVLAGLREGDVLVDLTTAGVTSARAVAARADARGARFVAC
EEEEEECCCHHHHHHHCCCCHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEE
PVLGSKTAAEQAQVVLVAGGPGPARERLRPALHAISARLFELDDPAQAALMKLCVNAVGG
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AMMTAFGEAVALASAGGVEPSRLVEVLQASAFHSPLYLMKGELVQKKDWAPRFRIALAEK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCCEEEEEHHH
DQRLAQEAAAELGAQVPVSEAVRELMAAATEAGRGDQDVAALADLYLEWTRRR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]