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The map label for this gene is zraS [H]

Identifier: 220915723

GI number: 220915723

Start: 679668

End: 681218

Strand: Reverse

Name: zraS [H]

Synonym: A2cp1_0604

Alternate gene names: 220915723

Gene position: 681218-679668 (Counterclockwise)

Preceding gene: 220915725

Following gene: 220915722

Centisome position: 13.54

GC content: 74.85

Gene sequence:

>1551_bases
ATGCGACGCGTCGCCACCAAGATCTTCCTCACCTTCGCGGTCGCCCTGGCGGCGTTCGCGGCGGTGGCCGGGTTCGGTGT
GGCGCGCCTCCACGATCTGCGGCGCGACCTGCGCACGCTGTCCTCCGGCTACATCCCGCTCACCCGGATCGCGGCGCAGC
TCGACGTGAAGGACTGGGTCACCGCCCGCGCGCTGGAGGCGCGGGTGCTCGACCCCGCCGCCCGGCGGGCCTACCTCCCG
GTGGCGAGGGCGCACTTCCCCGCGCTGGTGCGCGAGAAGATCGAGGAGGGCAAGCGCGTGGCCGCCGGCGCGCGGCTCAA
CGCCTCCGGCGACGACGCCCACTTCCTCGGCGACGTGCTCGCCCGGCTGGACGGCCTCGCCGCGCGATGGGCCGACTACG
ATCGCGAGGCGCGGGCGCTGCTCGACGCGCTGGAGGGCGGGGCGGGCCTGGGCCCGGGCGCGGCGGCGTTCGACGCGCGC
GTGGCCGCGGTGCGGCAGCTGGAGAAGGGGCTCAGCCTGGACGTGAAGCTGCTGCAGGTCGCGCTCGAGACGCAGATCGC
CGACCGCGTGCACGCCGCCGAGCGGACCGAGGGGAACACCGTCGCGCTGACGGTGATCTACTCCCTGCTCGCGCTGGCGG
TGGGCGTCGGCGCGGCGGCCATCTCGCAGCGGCTGCTCGCGCCCATCCAGACCTTGACCGAGGGCGTGAAGGCGGTCGCG
GCCGGCGACCTCACCCGCAAGGTGGAGGCGCGCGGCCGGGACGAGATCTCGGTGCTCGCGCACGAGTTCAACGCCATGGC
GGCCTCGCTCGACCGGCAGCGCGCCGACCTGCTCCGGGCGGAGCGGCTGGCCGCGGTCGGCCGCATCTCGGCGCAGATCA
CGCACGAGATCCGGAACCCGCTGAACGCCATCGGCCTGAACGCCGAGCTGCTCGCCGAGGAGATCGCCGCCCTGCCCGCG
CCGCCGGCCGAGGCGACCGGGCTGGTGTCCGCGATCTCGCGCGAGGTGGACCGGCTGAACGCGGTGACCGAGGAGTACCT
CCGCTTCGCGCGCCTCCCGCGCCCGCAGCTCGAGCGCCACGACCTGAACGAGATCCTGGGCGGCCTGCTCGACTTCCTCG
GGCCCGAGCTCGCCGCCGCGCGCGTGGCGGTGCACCGCGCCCTGGCGCCCGGCCTGCCCGGCGTGCGCTGCGACGAGGGG
CAGCTCCGCGCGGTGTTCCTGAACCTCCTGCGGAACAGCCGCGAGGCGATGCCCGGAGGCGGGACCGTGACCGTGGTGAC
CCGCCGGGCGGACTCCGGCGCGGCCGTCGAGGCCGAGCTGGGGGACACCGGGGGGGGGATCCCCCCAGGTGACCTGACGC
GGATCTTCGAGCCGTTCTACTCCACGAAGGAACGCGGCACCGGCCTGGGGCTCGCGTTCACCCAGCAGGTCGTCGAGGAG
CACGGCGGGACCATCCGGTGCGAGAGCACCGTGGGCCGGGGAACGATCTTCACCCTCCGCTTCCCGGCGGTCCAGGACAG
GGTGGAACACGAGGTGGGTGCAGCCACATGA

Upstream 100 bases:

>100_bases
CCATGCGAGCGGGCGCCCGGCGCGGCGCTTGCGGGTGGCGCGGGTTAGGAACGTTCCCGCCCGCCGCCGGGCCGGGCACG
GTAGGATCGCCGGCTCCCCC

Downstream 100 bases:

>100_bases
GGCGGTCGAGCCGGCAGGTGCGGGTGAAGCAGCGCAAGGATGCGTCGGAGAAGCGGGGCGGCGCGTTCCGCGTGGCGCTG
CCGCGCGCCCGGGTGGCGGC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MRRVATKIFLTFAVALAAFAAVAGFGVARLHDLRRDLRTLSSGYIPLTRIAAQLDVKDWVTARALEARVLDPAARRAYLP
VARAHFPALVREKIEEGKRVAAGARLNASGDDAHFLGDVLARLDGLAARWADYDREARALLDALEGGAGLGPGAAAFDAR
VAAVRQLEKGLSLDVKLLQVALETQIADRVHAAERTEGNTVALTVIYSLLALAVGVGAAAISQRLLAPIQTLTEGVKAVA
AGDLTRKVEARGRDEISVLAHEFNAMAASLDRQRADLLRAERLAAVGRISAQITHEIRNPLNAIGLNAELLAEEIAALPA
PPAEATGLVSAISREVDRLNAVTEEYLRFARLPRPQLERHDLNEILGGLLDFLGPELAAARVAVHRALAPGLPGVRCDEG
QLRAVFLNLLRNSREAMPGGGTVTVVTRRADSGAAVEAELGDTGGGIPPGDLTRIFEPFYSTKERGTGLGLAFTQQVVEE
HGGTIRCESTVGRGTIFTLRFPAVQDRVEHEVGAAT

Sequences:

>Translated_516_residues
MRRVATKIFLTFAVALAAFAAVAGFGVARLHDLRRDLRTLSSGYIPLTRIAAQLDVKDWVTARALEARVLDPAARRAYLP
VARAHFPALVREKIEEGKRVAAGARLNASGDDAHFLGDVLARLDGLAARWADYDREARALLDALEGGAGLGPGAAAFDAR
VAAVRQLEKGLSLDVKLLQVALETQIADRVHAAERTEGNTVALTVIYSLLALAVGVGAAAISQRLLAPIQTLTEGVKAVA
AGDLTRKVEARGRDEISVLAHEFNAMAASLDRQRADLLRAERLAAVGRISAQITHEIRNPLNAIGLNAELLAEEIAALPA
PPAEATGLVSAISREVDRLNAVTEEYLRFARLPRPQLERHDLNEILGGLLDFLGPELAAARVAVHRALAPGLPGVRCDEG
QLRAVFLNLLRNSREAMPGGGTVTVVTRRADSGAAVEAELGDTGGGIPPGDLTRIFEPFYSTKERGTGLGLAFTQQVVEE
HGGTIRCESTVGRGTIFTLRFPAVQDRVEHEVGAAT
>Mature_516_residues
MRRVATKIFLTFAVALAAFAAVAGFGVARLHDLRRDLRTLSSGYIPLTRIAAQLDVKDWVTARALEARVLDPAARRAYLP
VARAHFPALVREKIEEGKRVAAGARLNASGDDAHFLGDVLARLDGLAARWADYDREARALLDALEGGAGLGPGAAAFDAR
VAAVRQLEKGLSLDVKLLQVALETQIADRVHAAERTEGNTVALTVIYSLLALAVGVGAAAISQRLLAPIQTLTEGVKAVA
AGDLTRKVEARGRDEISVLAHEFNAMAASLDRQRADLLRAERLAAVGRISAQITHEIRNPLNAIGLNAELLAEEIAALPA
PPAEATGLVSAISREVDRLNAVTEEYLRFARLPRPQLERHDLNEILGGLLDFLGPELAAARVAVHRALAPGLPGVRCDEG
QLRAVFLNLLRNSREAMPGGGTVTVVTRRADSGAAVEAELGDTGGGIPPGDLTRIFEPFYSTKERGTGLGLAFTQQVVEE
HGGTIRCESTVGRGTIFTLRFPAVQDRVEHEVGAAT

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=35.6828193832599, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=34.0248962655602, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788393, Length=292, Percent_Identity=28.0821917808219, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789808, Length=330, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789149, Length=314, Percent_Identity=27.7070063694268, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI145693157, Length=227, Percent_Identity=29.0748898678414, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790300, Length=253, Percent_Identity=30.0395256916996, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI48994928, Length=236, Percent_Identity=30.5084745762712, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=27.8026905829596, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=319, Percent_Identity=26.9592476489028, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081816, Length=230, Percent_Identity=29.1304347826087, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=292, Percent_Identity=26.027397260274, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786912, Length=220, Percent_Identity=27.7272727272727, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790346, Length=290, Percent_Identity=23.448275862069, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54929; Mature: 54929

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVATKIFLTFAVALAAFAAVAGFGVARLHDLRRDLRTLSSGYIPLTRIAAQLDVKDWV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TARALEARVLDPAARRAYLPVARAHFPALVREKIEEGKRVAAGARLNASGDDAHFLGDVL
HHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHCCCEECCCCCHHHHHHHHH
ARLDGLAARWADYDREARALLDALEGGAGLGPGAAAFDARVAAVRQLEKGLSLDVKLLQV
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALETQIADRVHAAERTEGNTVALTVIYSLLALAVGVGAAAISQRLLAPIQTLTEGVKAVA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGDLTRKVEARGRDEISVLAHEFNAMAASLDRQRADLLRAERLAAVGRISAQITHEIRNP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
LNAIGLNAELLAEEIAALPAPPAEATGLVSAISREVDRLNAVTEEYLRFARLPRPQLERH
HHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHC
DLNEILGGLLDFLGPELAAARVAVHRALAPGLPGVRCDEGQLRAVFLNLLRNSREAMPGG
CHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
GTVTVVTRRADSGAAVEAELGDTGGGIPPGDLTRIFEPFYSTKERGTGLGLAFTQQVVEE
CEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
HGGTIRCESTVGRGTIFTLRFPAVQDRVEHEVGAAT
CCCEEEEECCCCCCEEEEEECCHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRVATKIFLTFAVALAAFAAVAGFGVARLHDLRRDLRTLSSGYIPLTRIAAQLDVKDWV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TARALEARVLDPAARRAYLPVARAHFPALVREKIEEGKRVAAGARLNASGDDAHFLGDVL
HHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHCCCEECCCCCHHHHHHHHH
ARLDGLAARWADYDREARALLDALEGGAGLGPGAAAFDARVAAVRQLEKGLSLDVKLLQV
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALETQIADRVHAAERTEGNTVALTVIYSLLALAVGVGAAAISQRLLAPIQTLTEGVKAVA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGDLTRKVEARGRDEISVLAHEFNAMAASLDRQRADLLRAERLAAVGRISAQITHEIRNP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
LNAIGLNAELLAEEIAALPAPPAEATGLVSAISREVDRLNAVTEEYLRFARLPRPQLERH
HHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHC
DLNEILGGLLDFLGPELAAARVAVHRALAPGLPGVRCDEGQLRAVFLNLLRNSREAMPGG
CHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
GTVTVVTRRADSGAAVEAELGDTGGGIPPGDLTRIFEPFYSTKERGTGLGLAFTQQVVEE
CEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
HGGTIRCESTVGRGTIFTLRFPAVQDRVEHEVGAAT
CCCEEEEECCCCCCEEEEEECCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]