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The map label for this gene is bar [H]

Identifier: 220915691

GI number: 220915691

Start: 648935

End: 649528

Strand: Direct

Name: bar [H]

Synonym: A2cp1_0572

Alternate gene names: 220915691

Gene position: 648935-649528 (Clockwise)

Preceding gene: 220915689

Following gene: 220915692

Centisome position: 12.9

GC content: 75.76

Gene sequence:

>594_bases
ATGAGGCAGATCCGGATGGCCACGCTCGACGACGCCGGGGACGTCGCGGAGATCTACGGCGCGGTGGTGGCGGGCACGCC
CATCTCGTTCGAGCTGGAGCCGCCCGGTCCCGGGGAGATGGCGCGCCGGATGGCGGCGGTGCTCGAGCTGGCGCCGTGGC
TGGTGTGCGAGGAGGACGGCCGGGTGGACGGGTACGTCTACGCCTCGCGCCACCACGATCGCGCTGCCTACCGCTGGAGC
GTGGACGTGACGGTGTACGTGCGCGACGGCCGCCGCCGCGGCGGCCTCGGGCGCGCGCTCTACACCGCGCTGCTCGAGCT
GCTCCGCGCGCAGGGGTTCCACGCCGCCCACGCCGGGATCACGCTGCCGAACGCCGGGAGCGTGGGGCTGCACGAGGCGC
TCGGCTTCCGGCCCATCGGCGTGTACCCGCGCGTCGGCTGGAAGATGGGCGCGTGGCACGACGTCGGCTACTGGCAGCTC
GAGCTGCGCGAGCGCACCCGCGCCCCGGGGCCGATCCTCGCGGTGGATGCGCTCCGGCGCTCGCCGGCGTGGGATCGCGC
CCTCGCCGCCGGCCAGGTGCTGCTCGCGCGGTAG

Upstream 100 bases:

>100_bases
GAGGACGTCGATGCGAACTATATGGCGCATCGGGTGTGTCAATCGAGTGCCGGCGCTATGTTGAGCGGGTCCGGGGCCGC
ACCGCCCCGCGGAGGACGCC

Downstream 100 bases:

>100_bases
CCGCCGCGCGCCGCCCGCCGGTCCCTCGGTCCGTGCCCACGCCGTCGCGCCCCGGGTGTATGGATGGCCGGTCCGGCGGC
GCGCACGCGGCGCGGCGCGC

Product: GCN5-like N-acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MRQIRMATLDDAGDVAEIYGAVVAGTPISFELEPPGPGEMARRMAAVLELAPWLVCEEDGRVDGYVYASRHHDRAAYRWS
VDVTVYVRDGRRRGGLGRALYTALLELLRAQGFHAAHAGITLPNAGSVGLHEALGFRPIGVYPRVGWKMGAWHDVGYWQL
ELRERTRAPGPILAVDALRRSPAWDRALAAGQVLLAR

Sequences:

>Translated_197_residues
MRQIRMATLDDAGDVAEIYGAVVAGTPISFELEPPGPGEMARRMAAVLELAPWLVCEEDGRVDGYVYASRHHDRAAYRWS
VDVTVYVRDGRRRGGLGRALYTALLELLRAQGFHAAHAGITLPNAGSVGLHEALGFRPIGVYPRVGWKMGAWHDVGYWQL
ELRERTRAPGPILAVDALRRSPAWDRALAAGQVLLAR
>Mature_197_residues
MRQIRMATLDDAGDVAEIYGAVVAGTPISFELEPPGPGEMARRMAAVLELAPWLVCEEDGRVDGYVYASRHHDRAAYRWS
VDVTVYVRDGRRRGGLGRALYTALLELLRAQGFHAAHAGITLPNAGSVGLHEALGFRPIGVYPRVGWKMGAWHDVGYWQL
ELRERTRAPGPILAVDALRRSPAWDRALAAGQVLLAR

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=165, Percent_Identity=36.3636363636364, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 21665; Mature: 21665

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIRMATLDDAGDVAEIYGAVVAGTPISFELEPPGPGEMARRMAAVLELAPWLVCEEDG
CCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEECCCC
RVDGYVYASRHHDRAAYRWSVDVTVYVRDGRRRGGLGRALYTALLELLRAQGFHAAHAGI
CCCEEEEECCCCCCEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCE
TLPNAGSVGLHEALGFRPIGVYPRVGWKMGAWHDVGYWQLELRERTRAPGPILAVDALRR
ECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEECCCEEEEEEEHHCCCCCCEEEEHHHHC
SPAWDRALAAGQVLLAR
CCCHHHHHHHCEEEECC
>Mature Secondary Structure
MRQIRMATLDDAGDVAEIYGAVVAGTPISFELEPPGPGEMARRMAAVLELAPWLVCEEDG
CCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEECCCC
RVDGYVYASRHHDRAAYRWSVDVTVYVRDGRRRGGLGRALYTALLELLRAQGFHAAHAGI
CCCEEEEECCCCCCEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCE
TLPNAGSVGLHEALGFRPIGVYPRVGWKMGAWHDVGYWQLELRERTRAPGPILAVDALRR
ECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEECCCEEEEEEEHHCCCCCCEEEEHHHHC
SPAWDRALAAGQVLLAR
CCCHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2315036 [H]