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The map label for this gene is gcvA [H]

Identifier: 220915674

GI number: 220915674

Start: 634302

End: 635216

Strand: Direct

Name: gcvA [H]

Synonym: A2cp1_0555

Alternate gene names: 220915674

Gene position: 634302-635216 (Clockwise)

Preceding gene: 220915673

Following gene: 220915675

Centisome position: 12.61

GC content: 80.55

Gene sequence:

>915_bases
GTGAAGGACCTCCCCCTGAACGCGCTCCGCGCCTTCGCGCTCGTGTTCGCGCACCGCGGCGTGCGCCCCGCCGCGCGCGA
GCTCGGCGTGGCCCACTCGTCGCTGAGCCGGCACATGGCCGAGCTGGAGGCCTGGGCCGGCACCCCGCTTACCCGCGGCC
CCGGCGGGCGGGCCGGGCTCTCGTTCACGCCGCAGGGCGAGGCGCTCGGCCAGGCGGCGCTCGCCGCGCTCCGCGAGCTG
GAGCGGGCCGCTGCCGCGGTGCGGGAGGCGCGCTCGGAGCGCTCGGTGGTGATCTCGACCACCGCCTCGTTCGCGAGCCG
CTGGCTCCTGCCGCGGCTCCCGGCGCTGGAGGCGCGCCACCCGGACCTGGAGGTCTCGGTGCTGGTGGACCCGCGGCCGG
TGGACCTCGGGACCGCGGGCGCCGGGGAGGTGGACCTCGCGATCCGGCTGGGGCGCGGCCCCTGGCGCGACCTGGACTGC
CAGCCGCTGCTCGACGAGGTGCTGTACCCGGTGATGAGCCCGGCCGCCTGGCGCGCGGCGGGCTCGCCGCGCCGGCCGCC
GGCGCTGGCGCGGCTGCGGCTCCTGCACGACCGCGATCCGGGCGCGACCTGGGAGCTGTGGCGACGCGCCCATGGGCCCG
CCTCGCTGGACGTCCGGCGCGGCCCGCGCCTCGCCTCGACCGACCTCGTGCTCCGCGCCGCGGCGCAGGGGCAGGGGGTG
GCGCTGGCGCGGCACCGGCTCGCCGCGGACGACCTGGCGGCCGGCCTGCTGGTCCGGCCCTTCGAGCGGTCGGTGGCGCT
CGGCCCCGCCTACTGGCTGGTGCTCCCGCCGCACGCGCGCGGCCGCGCCGCCACCGCGGGGGTGGTGGCCTGGCTGGAGC
AGGAGGCCCGCGCCTGCGGGCCGCTCCCGGCCTGA

Upstream 100 bases:

>100_bases
CGACTGCGCCCCGCCGCGCGGCGCGGACTGCGGCGGCCTGTGCGCACCGGCACCCGCCGCGGGCGCGCGCTGAGCGGGGC
TTCGGTCTATCCTCCCGACC

Downstream 100 bases:

>100_bases
ACGGTCTCGTCCCGAGACCGAACCTCGCGCCCGTCCGACCGGCCCGCGCCGCAGATCCCCGGCATGGAGGGCGCCGGCAT
GGAGACGTGGATCGCGACCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKDLPLNALRAFALVFAHRGVRPAARELGVAHSSLSRHMAELEAWAGTPLTRGPGGRAGLSFTPQGEALGQAALAALREL
ERAAAAVREARSERSVVISTTASFASRWLLPRLPALEARHPDLEVSVLVDPRPVDLGTAGAGEVDLAIRLGRGPWRDLDC
QPLLDEVLYPVMSPAAWRAAGSPRRPPALARLRLLHDRDPGATWELWRRAHGPASLDVRRGPRLASTDLVLRAAAQGQGV
ALARHRLAADDLAAGLLVRPFERSVALGPAYWLVLPPHARGRAATAGVVAWLEQEARACGPLPA

Sequences:

>Translated_304_residues
MKDLPLNALRAFALVFAHRGVRPAARELGVAHSSLSRHMAELEAWAGTPLTRGPGGRAGLSFTPQGEALGQAALAALREL
ERAAAAVREARSERSVVISTTASFASRWLLPRLPALEARHPDLEVSVLVDPRPVDLGTAGAGEVDLAIRLGRGPWRDLDC
QPLLDEVLYPVMSPAAWRAAGSPRRPPALARLRLLHDRDPGATWELWRRAHGPASLDVRRGPRLASTDLVLRAAAQGQGV
ALARHRLAADDLAAGLLVRPFERSVALGPAYWLVLPPHARGRAATAGVVAWLEQEARACGPLPA
>Mature_304_residues
MKDLPLNALRAFALVFAHRGVRPAARELGVAHSSLSRHMAELEAWAGTPLTRGPGGRAGLSFTPQGEALGQAALAALREL
ERAAAAVREARSERSVVISTTASFASRWLLPRLPALEARHPDLEVSVLVDPRPVDLGTAGAGEVDLAIRLGRGPWRDLDC
QPLLDEVLYPVMSPAAWRAAGSPRRPPALARLRLLHDRDPGATWELWRRAHGPASLDVRRGPRLASTDLVLRAAAQGQGV
ALARHRLAADDLAAGLLVRPFERSVALGPAYWLVLPPHARGRAATAGVVAWLEQEARACGPLPA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=273, Percent_Identity=32.967032967033, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=27.3333333333333, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786448, Length=298, Percent_Identity=27.5167785234899, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081978, Length=267, Percent_Identity=29.9625468164794, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32510; Mature: 32510

Theoretical pI: Translated: 11.47; Mature: 11.47

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLPLNALRAFALVFAHRGVRPAARELGVAHSSLSRHMAELEAWAGTPLTRGPGGRAGL
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
SFTPQGEALGQAALAALRELERAAAAVREARSERSVVISTTASFASRWLLPRLPALEARH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCC
PDLEVSVLVDPRPVDLGTAGAGEVDLAIRLGRGPWRDLDCQPLLDEVLYPVMSPAAWRAA
CCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHCC
GSPRRPPALARLRLLHDRDPGATWELWRRAHGPASLDVRRGPRLASTDLVLRAAAQGQGV
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHCCCCCH
ALARHRLAADDLAAGLLVRPFERSVALGPAYWLVLPPHARGRAATAGVVAWLEQEARACG
HHHHHHHHHHHHHHHHHHCCHHHCEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
PLPA
CCCC
>Mature Secondary Structure
MKDLPLNALRAFALVFAHRGVRPAARELGVAHSSLSRHMAELEAWAGTPLTRGPGGRAGL
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
SFTPQGEALGQAALAALRELERAAAAVREARSERSVVISTTASFASRWLLPRLPALEARH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCC
PDLEVSVLVDPRPVDLGTAGAGEVDLAIRLGRGPWRDLDCQPLLDEVLYPVMSPAAWRAA
CCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHCC
GSPRRPPALARLRLLHDRDPGATWELWRRAHGPASLDVRRGPRLASTDLVLRAAAQGQGV
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHCCCCCH
ALARHRLAADDLAAGLLVRPFERSVALGPAYWLVLPPHARGRAATAGVVAWLEQEARACG
HHHHHHHHHHHHHHHHHHCCHHHCEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
PLPA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]