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The map label for this gene is ubiE [H]

Identifier: 220915522

GI number: 220915522

Start: 453272

End: 453979

Strand: Reverse

Name: ubiE [H]

Synonym: A2cp1_0403

Alternate gene names: 220915522

Gene position: 453979-453272 (Counterclockwise)

Preceding gene: 220915523

Following gene: 220915521

Centisome position: 9.03

GC content: 74.44

Gene sequence:

>708_bases
GTGAGCGTGAACGTGCCGCTGCCCGGCGAGGCGCACCGCGGCTCCGCGGTCCGGGCCATGTTCGATCGAATCGCCCCGCG
CTACGACCTGCTGAACCGGGTCATGACGCTGAAGGTGGACCAGGCCTGGCGCCGCCGCCTGCTCTCCGACCTCGCCCCGA
AGGACGGCGAGCGGATGCTGGACCTGTGCGCCGGGACCATGGACGTCGCCGACCTGGCCCGGCGCCGCGCCCCGGGCCTG
CGCGTCACCGGCGCCGACTTCTCCATGCAGATGCTCCGCCGCGGGGTGGAGAAGACCGCCCTGCCCGCGTCGCAGGCGGA
CGCGATGGCGCTGCCGTTCCTGGACGCGCGCTTCGACCTCGCCACGGTGACGTTCGGCATGCGCAACCTGGAGCGCTACG
AGGTAGGCCTGGCCGAGCTGGCGCGGGTGCTGCGTCCGGGCGGCCGCCTCGGCGTGCTCGAGTTCTTCCGCTCCGAGTCG
CGCGGCTCGCGCCTCGTGCACGGTGCGTACAACCGGCTGGCGCTGCCGGTGCTGGGCCGCATCCTCTCGCCCGACCCGGA
GGCGTACCGCTACCTGGTGGCCTCGATGGAGCGCTTCGCCTCGCGGGTCGAGTTCGAGGACGCGGCGCGCCGCGCCGGCT
TCCGCGAGGTGCGCGGCGAGACGCTGTTCCCGGGCGTGTGCGGGCTCGTCACGGCGGTGCGCGCGTGA

Upstream 100 bases:

>100_bases
TCTACGACCGGCTGGTGCCGGGCGCGGCGCCGCTCCGCCGCCCGCGCATCGCCCCGCCCCCGGCGGCGTCCTGGACGCCG
CGCCGCGGAGGGATCCTCCA

Downstream 100 bases:

>100_bases
AGCTGGTGGTCGCCGTCTCCGGCGCGTCGGGCGCCCCCTACGCGAGGCGGCTCCTCGACTTCCTGGCCGCGAACGCGGAC
CTGGGCGTGTCGGCCGACCT

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSVNVPLPGEAHRGSAVRAMFDRIAPRYDLLNRVMTLKVDQAWRRRLLSDLAPKDGERMLDLCAGTMDVADLARRRAPGL
RVTGADFSMQMLRRGVEKTALPASQADAMALPFLDARFDLATVTFGMRNLERYEVGLAELARVLRPGGRLGVLEFFRSES
RGSRLVHGAYNRLALPVLGRILSPDPEAYRYLVASMERFASRVEFEDAARRAGFREVRGETLFPGVCGLVTAVRA

Sequences:

>Translated_235_residues
MSVNVPLPGEAHRGSAVRAMFDRIAPRYDLLNRVMTLKVDQAWRRRLLSDLAPKDGERMLDLCAGTMDVADLARRRAPGL
RVTGADFSMQMLRRGVEKTALPASQADAMALPFLDARFDLATVTFGMRNLERYEVGLAELARVLRPGGRLGVLEFFRSES
RGSRLVHGAYNRLALPVLGRILSPDPEAYRYLVASMERFASRVEFEDAARRAGFREVRGETLFPGVCGLVTAVRA
>Mature_234_residues
SVNVPLPGEAHRGSAVRAMFDRIAPRYDLLNRVMTLKVDQAWRRRLLSDLAPKDGERMLDLCAGTMDVADLARRRAPGLR
VTGADFSMQMLRRGVEKTALPASQADAMALPFLDARFDLATVTFGMRNLERYEVGLAELARVLRPGGRLGVLEFFRSESR
GSRLVHGAYNRLALPVLGRILSPDPEAYRYLVASMERFASRVEFEDAARRAGFREVRGETLFPGVCGLVTAVRA

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=266, Percent_Identity=30.0751879699248, Blast_Score=135, Evalue=2e-32,
Organism=Escherichia coli, GI48994983, Length=228, Percent_Identity=32.0175438596491, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17557039, Length=234, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323526, Length=246, Percent_Identity=30.4878048780488, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24641702, Length=246, Percent_Identity=31.7073170731707, Blast_Score=130, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26139; Mature: 26008

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNVPLPGEAHRGSAVRAMFDRIAPRYDLLNRVMTLKVDQAWRRRLLSDLAPKDGERML
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DLCAGTMDVADLARRRAPGLRVTGADFSMQMLRRGVEKTALPASQADAMALPFLDARFDL
HHHHCHHHHHHHHHHCCCCCEEECCCHHHHHHHCCHHHHCCCCCCCCHHHCCHHHHHHHH
ATVTFGMRNLERYEVGLAELARVLRPGGRLGVLEFFRSESRGSRLVHGAYNRLALPVLGR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ILSPDPEAYRYLVASMERFASRVEFEDAARRAGFREVRGETLFPGVCGLVTAVRA
HCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SVNVPLPGEAHRGSAVRAMFDRIAPRYDLLNRVMTLKVDQAWRRRLLSDLAPKDGERML
CCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DLCAGTMDVADLARRRAPGLRVTGADFSMQMLRRGVEKTALPASQADAMALPFLDARFDL
HHHHCHHHHHHHHHHCCCCCEEECCCHHHHHHHCCHHHHCCCCCCCCHHHCCHHHHHHHH
ATVTFGMRNLERYEVGLAELARVLRPGGRLGVLEFFRSESRGSRLVHGAYNRLALPVLGR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ILSPDPEAYRYLVASMERFASRVEFEDAARRAGFREVRGETLFPGVCGLVTAVRA
HCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA